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Chapter 28 : Interaction of with

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Abstract:

Several pathogens exhibit a considerable host range. , for example, can infect various protozoa species, experimentally inoculated guinea pigs, and human macrophages, as well as epithelial cells. Vegetative cells of feed on bacteria and upon starvation aggregate and differentiate into pluricellular fruiting bodies. To evaluate whether is a suitable model system for studying Legionella pathogenicity, the authors compared the intracellular growth of different species in with the established host model system . The FlaA-and the Mip-negative mutant of Corby revealed moderate growth defects and the A-negative mutant was severely impaired to grow intracellularly. To examine host functions required for growth, the authors also investigated defined mutants. Identification of spp., spp., and spp. by fluorescence-labeled rRNA probes have been provided in this chapter. Available molecular tools to manipulate the host are transformation with integrating and nonintegrating eukaryotic vectors, homologous recombination, antisense techniques, and restriction enzyme mediated integration (REMI). The application of these methods should allow the elucidation of the interaction of bacterial virulence factors with specific host targets.

Citation: Steinert M, Hagele S, Skriwan C, Crimm D, Fajardo M, Heuner K, Hentschel U, Hacker J, Schleicher M, Ludwig W, Mane R. 2002. Interaction of with , p 161-164. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch28

Key Concept Ranking

Dictyostelium discoideum
0.56349206
Legionella pneumophila
0.5377081
0.56349206
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Figures

Image of FIGURE 1
FIGURE 1

Transmission electron micrograph of within a single vacuole of after 48 h of coincubation. Bar, 1 m.

Citation: Steinert M, Hagele S, Skriwan C, Crimm D, Fajardo M, Heuner K, Hentschel U, Hacker J, Schleicher M, Ludwig W, Mane R. 2002. Interaction of with , p 161-164. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch28
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References

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1. Abu Kwaik, Y. 1998. Fatal attraction of mammalian cells to Legionella pneumophila. Mol. Microbiol. 30: 689 695.
2. Abu Kwaik, Y.,, L.-Y. Gao,, B. J. Stone, C. Venkataraman, and O. S. Harb. 1998. Invasion of protozoa by Legionella pneumophila and its role in bacterial ecology and pathogenesis. Appl. Environ. Microbiol. 64: 3127 3133.
3. Beckers, M. C.,, S. Yoshida,, K. Morgan,, E. Skamene,, and P. Gros. 1995. Natural resistance to infection with Legionella pneumophila: chromosomal localization of the lgn1 susceptibility gene. Mamm. Genome 6: 540 545.
4. Coers, J.,, C. Monahan,, and C. R. Roy. 1999. Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth. Nature Cell Biol. 1: 451 453.
5. Dietrich, C.,, K. Heuner,, B. Brand,, J. Hacker,, and M. Steinert. 2001. Flagellum of Legionella pneumophila positively affects the early phase of infection of eukaryotic host cells. Infect. Immun. 69: 2116 2122.
6. Fettes, P. S.,, M. Susa,, J. Hacker,, and R. Marre. 2000. Characterization of the Legionella pneumophila gene ligA. Int. J. Med. Microbiol. 290: 239 250.
7. Fields, B. 1996. The molecular ecology of le-gionellae. Trends Microbiol. 7: 286 290.
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10. Grimm, D.,, H. Merkert,, W. Ludwig,, K. H. Schleifer,, J. Hacker,, and B. C. Brand. 1998. Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe. Appl. Environ. Microbiol. 64: 2686 2690.
11. Hägele, S.,, R. Köhler,, H. Merkert,, M. Schleicher,, J. Hacker,, and M. Steinert. 2000. Dictyostelium discoideum: a new host model system for intracellular pathogens of the genus Legionella. Cell. Microbiol. 2: 165 171.
12. Helbig, J. H.,, P. C. Lück,, M. Steinert,, E. Jacobs,, and M. Witt. 2001. Immunlocalization of Mip protein of extracellularly and intracellu-larly grown Legionella pneumophila. Lett. Appl. Microbiol. 32: 83 88.
13. Hentschel, U.,, M. Steinert,, and J. Hacker. 2000. Common molecular mechanisms of symbiosis and pathogenesis. Trends Microbiol. 8: 226 231.
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15. Köhler, R.,, A. Bubert,, W. Goebel,, M. Steinert,, J. Hacker,, and B. Bubert. 2000. Expression and use of the green fluorescent protein as a reporter system in Legionella pneumophila. Mol. Gen. Genet. 262: 1060 1069.
16. Mutzel, R. 1995. Molecular biology, growth and development of the cellular slime mold Dictyostelium discoideum. Experientia 51: 1103 1109.
17. Steinert, M.,, U. Hentschel,, and J. Hacker. 2000. Symbiosis and pathogenesis: evolution of the microbe-host interaction. Naturwissenschaften 87: 1 11.
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21. Wintermeyer, E.,, B. Ludwig,, M. Steinert,, B. Schmidt,, G. Fischer,, and J. Hacker. 1995. Influence of site specifically altered Mip proteins on intracellular survival of Legionella pneumophila in eukaryotic cells. Infect. Immun. 63: 4576 4583.

Tables

Generic image for table
TABLE 1

IntraceUular growth of in and

Citation: Steinert M, Hagele S, Skriwan C, Crimm D, Fajardo M, Heuner K, Hentschel U, Hacker J, Schleicher M, Ludwig W, Mane R. 2002. Interaction of with , p 161-164. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch28
Generic image for table
TABLE 2

Identification of spp., spp., and spp. by fluorescence-labeled rRNA probes

Citation: Steinert M, Hagele S, Skriwan C, Crimm D, Fajardo M, Heuner K, Hentschel U, Hacker J, Schleicher M, Ludwig W, Mane R. 2002. Interaction of with , p 161-164. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch28

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