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Chapter 30 : Impact of Amoebae, Bacteria, and on Multiplication and Distribution in an Aquatic Environment

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Impact of Amoebae, Bacteria, and on Multiplication and Distribution in an Aquatic Environment, Page 1 of 2

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Abstract:

This chapter aims at defining a minimum multiplicity of infection (MOI) and determining if the presence of non- species of bacteria would influence the recovery of CFU during replication and lysis of amoebae host cells. It also aims at documenting the impact of adding the free-swimming ciliate to the amplified number of after they had lysed the population of amoebae host cells. If amoebae infection by was the result of inadvertent contact and subsequent phagocytosis, an inhibitory effect might have been expected since addition of exogenous bacteria would likewise result in increased inadvertent contact with phagocytosis, and thus diminish potential contact and uptake of virulent . As a direct result of amplification, amoebae lysed, liberating the bacteria, which were then concentrated into vesicles by the sp. A conclusion reached in this study is that can initiate infection in amoebae at an MOI considerably lower than often used in laboratory studies.

Citation: McNealy T, Newsome A, Johnson R, Berk S. 2002. Impact of Amoebae, Bacteria, and on Multiplication and Distribution in an Aquatic Environment, p 170-175. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch30

Key Concept Ranking

Legionella pneumophila
0.7258334
Gram-Positive Bacteria
0.53118485
0.7258334
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Figures

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FIGURE 1

(A) The ciliate, , next to a cluster of expelled food vacuoles (vesicles). Bar, 5.0 m. (В) Large clusters of loosely attached vesicles released from after 24 h in coculture with amoebae and . Bar, 5.0 m.(С) Vesicles containing viable as determined by reduction of INT, which resulted in a dark-colored product within respiring bacteria. Bar, 5.0 m.

Citation: McNealy T, Newsome A, Johnson R, Berk S. 2002. Impact of Amoebae, Bacteria, and on Multiplication and Distribution in an Aquatic Environment, p 170-175. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch30
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References

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1. Abu Kwaik, Y.,, L.-Y. Gao,, B. J. Stone, C. Venkataraman, and O. S. Harb. 1998. Invasion of protozoa by Legionella pneumophila and its role in bacterial ecology and pathogenesis. Appl. Environ. Microbiol. 64:31273133.
2. Berk, S. B.,, R. S. Ting,, G. W. Turner,, and R. J. Ashburn. 1998. Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp. Appl. Environ. Microbiol. 64:279286.
3. Fields, B. S., 1993. Legionella and protozoa: interactions of a pathogen and its natural host, p. 437441. In J. M. Barbaree,, R. F. Breiman,, and A. P. Dufour (ed.), Legionella: Cunent Status and Emerging Perspectives. American Society of Microbiology, Washington, D.C.
4. Fliermans, C. B. 1996. Ecology of Legionella: from data to knowledge with a little wisdom. Microb. Ecol. 32:203228.
5. Harb, O. S.,, L.-Y. Gao,, and Y. Abu Kwaik. 2000. From protozoa to mammalian cells: a new paradigm in the life cycle of intracellular bacterial pathogens. Environ. Microbiol. 2:251265.
6. Harb, O. S.,, C. Venkataraman,, B. J. Haack,, L.-Y. Gao,, and Y. Abu Kwaik. 1998. Het-erogenity in the attachment and uptake mechanisms of the Legionnaires' disease bacterium, Legionella pneumophila, by protozoan hosts. Appl. Environ. Microbiol. 64:126132.
7. Rodriguez-Zaragoza, S. 1994. Ecology of free-living amoebae. Crit. Rev. Microbiol. 20:225241.
8. Rogers, J.,, A. B. Dowsett,, P. J. Dennis,, J. V. Lee,, and C. W. Keevil. 1994. Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora. Appl. Environ. Microbiol. 60:15851592.
9. Rogers, J.,, A. P. Dowsett,, P. J. Dennis,, J. V. Lee,, and C. W. Keevil. 1994. Influence of plumbing material on biofilm formation and growth of Legionella pneumophila in potable water systems. Appl. Environ. Microbiol. 60:18421851.
10. Stone, B. J.,, and Y. Abu Kwaik. 1998. Expression of multiple pili by Legionella pneumophila: identification and characterization of a type IV pilin gene and its role in adherence to mammalian and protozoan cells. Infect. Immun. 66:17681775.
11. Wright, J. B.,, I. Ruseska,, M. A. Athar,, S. Corbett,, and J. W. Coserton. 1989. Legionella pneumophila grows adherent to surfaces in vitro and in situ. Infect. Control Hosp. Epidemiol. 10: 408415.

Tables

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TABLE 1

Multiplication of in incubated in spring water at 30°C

Citation: McNealy T, Newsome A, Johnson R, Berk S. 2002. Impact of Amoebae, Bacteria, and on Multiplication and Distribution in an Aquatic Environment, p 170-175. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch30

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