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Chapter 38 : Comparison of Non-Serogroup 1 Detection by Biotest and Binax Legionella Urinary Antigen Enzyme Immunoassays

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Comparison of Non-Serogroup 1 Detection by Biotest and Binax Legionella Urinary Antigen Enzyme Immunoassays, Page 1 of 2

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Abstract:

The Binax enzyme immunoassay (EIA) and radioimmunoassay (RIA) both showed a certain cross-reactivity with non-serogroup 1 , as is to be expected when using polyclonal rabbit antibodies for the antigen detection. The Biotest EIA is the former urinary antigen EIA from the Robert Koch Institute in Berlin developed by Fehrenbach for extended cross-reactivity with non-serogroup 1 . Benson demonstrated that only 10 of 45 non-serogroup 1 urine samples previously positive in the broad-spectrum enzyme-linked immunosorbent assay described by Tang were positive with the Binax EIA (22%), whereas 13 of 42 of these samples were positive with the Biotest EIA (31%); when using a lower cut-off, 20 (48%) were positive with Biotest EIA. 28 non-serogroup 1 samples were evaluated from different countries provided by users of the test to demonstrate reactivity of the two urinary antigen tests with these urines. Binax EIA and Biotest EIA kits were used according to the test kit instructions. The Biotest EIA therefore demonstrates a considerably broader cross-reactivity with urines from non-serogroup 1 infections than the Binax EIA. Certainly more potential exists by fully exploiting the advantages of polyclonal antibodies from rabbits, broadly cross-reactive with species in the Biotest EIA, in continuing efforts to further improve reactivity with species other than .

Citation: Horn J. 2002. Comparison of Non-Serogroup 1 Detection by Biotest and Binax Legionella Urinary Antigen Enzyme Immunoassays, p 207-210. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch38

Key Concept Ranking

Enzyme-Linked Immunosorbent Assay
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Legionella pneumophila
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References

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1. Benson, R. F.,, W. T. Tang,, and B. S. Fields. 2000. Evaluation of the Binax and Biotest urinary antigen kits for detection of Legionnaires’ disease due to multiple serogroups and species of Legionella. J. Clin. Microbiol. 38:27632765.
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4. Hackmann, B. A.,, J. F. Plouffe,, R. F. Benson,, B. S. Fields,, and R. F. Breiman. 1996. Comparison of Binax Legionella EIA kit with Binax RIA urinary antigen kit for detection of Legionella pneumophila serogroup 1 antigen. J. Clin. Microbiol. 34:15791580.
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6. Kohler, R. B.,, S. E. Zimmermann,, E. Wilson,, S. D. Allen,, P. H. Edelstein,, I. J. Wheat,, and A. White. 1981. Rapid radioimmunoassay diagnosis of Legionnaires’ disease; detection and partial characterization of urinary antigen. Ann. Intern. Med. 94:601605.
7. Plouffe, J. F.,, M. File,, R. F. Breiman,, B. A. Hackmann,, S. J. Salstrom,, B. J. Marston,, and B. S. Fields. 1995. Reevaluation of the definition of Legionnaires’ disease: use of the urinary antigen assay. Clin. Infect. Dis. 20:12861291.
8. Rigby, E. W.,, J. F. Plouffe,, B. A. Hackmann,, D. S. Hill,, R. F. Benson,, and R. F. Breiman. 1997. Stability of Legionella urinary antigens over time. Diagn. Microbiol. Infect. Dis. 28:13.
9. Slack, M. P. E., 1999. Legionella ssp., p. 8.20.118.20.13. In D. Armstrong, and I. Cohen (ed.), Infectious Diseases. Harcourt Publishers Ltd., London, United Kingdom.
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11. Tang, P.,, and C. Krishnan,. 1993. Legionella antigenuria: six-year study of broad-spectrum enzyme-linked immunosorbent assay as a routine diagnostic test, p. 1213. In J. M. Barbaree,, R. F. Breiman,, and A. P. Dufour (ed.), Legionella: Current Status and Emerging Perspectives. American Society for Microbiology, Washington, D.C.

Tables

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TABLE 1

Titration of -spiked water samples with Biotest urinary antigen EIA

Citation: Horn J. 2002. Comparison of Non-Serogroup 1 Detection by Biotest and Binax Legionella Urinary Antigen Enzyme Immunoassays, p 207-210. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch38
Generic image for table
TABLE 2

Results for Biotest EIA and Binax EIA with urines from non-serogroup 1 infections

Citation: Horn J. 2002. Comparison of Non-Serogroup 1 Detection by Biotest and Binax Legionella Urinary Antigen Enzyme Immunoassays, p 207-210. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch38

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