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Chapter 42 : Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization

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Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization, Page 1 of 2

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Abstract:

In this chapter the authors report the application of fluorescence in situ hybridization (FISH) for the direct detection and identification of spp. and from respiratory tract specimens of patients with pneumonia using published 16S rRNA-based probes specific for the genus (Leg705) and for (LegPne1). To evaluate the usefulness of FISH as a clinical diagnostic method, the authors analyzed 160 respiratory samples (bronchoalvealar lavage (BAL), pleural fluid, sputum, and tissue) from patients suspected of having pneumonia caused by spp. Probes Leg705 and LegPne1, one labeled with fluorescein and the other labeled with Cy3, were added simultaneously to each well, except for the negative-control well, where hybridization buffer alone was added. Almost two-thirds of pneumonia cases identified were caused by either or . Given this background, development of a series of probes specific for the predominant species may be of value for identification and epidemiologic purposes.

Citation: Hu J, Limaye A, Fritsche T, Horn M, Juretschko S, Gautom R. 2002. Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization, p 221-224. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch42

Key Concept Ranking

Environmental Microbiology
0.76590514
Legionella pneumophila
0.76205635
0.76590514
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Figures

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FIGURE 1

Microscopic examination of BAL specimen after hybridization with probe LegPnel that is specific for was seen as a cluster of intracellular bacilli (arrowhead).

Citation: Hu J, Limaye A, Fritsche T, Horn M, Juretschko S, Gautom R. 2002. Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization, p 221-224. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch42
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References

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12. Plouffe, J. F.,, T. M. File, Jr.,, R. F. Breiman,, B. A. Hackman,, S. J. Salstrom,, B. J. Marston,, and B. S. Fields. 1995. Re-evaluation of the definition of Legionnaires' disease: use of the urinary antigen assay. Community Based Pneumonia Incidence Study Group. Clin. Infect. Dis. 20:12861291.
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Tables

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TABLE 1

Detection limit of spp. using FISH with probe LegPnel compared with culture on BCYE agar

Citation: Hu J, Limaye A, Fritsche T, Horn M, Juretschko S, Gautom R. 2002. Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization, p 221-224. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch42
Generic image for table
TABLE 2

Comparison of culture, DFA test, and FISH in patients with legionellosis

Citation: Hu J, Limaye A, Fritsche T, Horn M, Juretschko S, Gautom R. 2002. Direct Detection of Legionellae in Respiratory Tract Specimens by Using Fluorescence In Situ Hybridization, p 221-224. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch42

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