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Chapter 46 : Molecular Typing of Legionella Strains with Pulsed-Field Gel Electrophoresis and Random Primer-Amplified Polymorphic DNA in Nosocomial Legionnaires' Disease
Molecular Typing of Legionella Strains with Pulsed-Field Gel Electrophoresis and Random Primer-Amplified Polymorphic DNA in Nosocomial Legionnaires' Disease, Page 1 of 2< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555817985/9781555812300_Chap46-1.gif /docserver/preview/fulltext/10.1128/9781555817985/9781555812300_Chap46-2.gif
This chapter investigates the epidemiological relatedness between Legionella strains, isolated from a patient with Legionnaires' disease and from the hospital water supply. Strains of L. pneumophila serogroup 1 isolated from the patient and from the hospital environment, and clinical and environmental unrelated strains were typed by pulsed-field gel electrophoresis (PFGE) and by random amplified polymorphic DNA PCR (RAPD-PCR). L. pneumophila serogroup 1 strains isolated from the patient and 11 strains isolated from hot water samples collected from different sites of the hospital water supply showed the same profile by PFGE with Notl and Sfil. These strains shared identical profiles also by RAPD-PCR with the two primers used. The profiles of L. pneumophila serogroup 1 Philadelphia 1 type strain and of the clinical and environmental unrelated strains were different from the above strains by PFGE and by RAPD-PCR. The molecular typing results demonstrated that the strains of L. pneumophila serogroup 1, isolated from the patient and from the hospital hot water, were indistinguishable and thus genetically related, showing that the hospital water was the source of infection and thus confirming the nosocomial origin.
Key Concept Ranking
- Random Amplified Polymorphic DNA