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Chapter 47 : Multilocus Sequence Typing for Characterization of Serogroup 1 Isolates

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Multilocus Sequence Typing for Characterization of Serogroup 1 Isolates, Page 1 of 2

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Abstract:

Molecular typing of pathogenic relevant strains of serogroup 1 is indispensable in determining the epidemiology of the disease. In addition to classical serotyping and subtyping using monoclonal antibodies, a variety of molecular methods have been successfully employed for epidemiological purposes including amplified fragment length polymorphism (AFLP) analysis, pulsed-field gel electrophoresis, random amplified polymorphic DNA, and many other PCR-based methods. These techniques are currently used because of their feasibility, rapidity, and great potential for discrimination. Multilocus sequence typing (MLST) is a sequence-based variation of multilocus enzyme electrophoresis (MLEE), where several genes are compared simultaneously at their sequence level. In the future, the authors plan to validate MLST method for the whole panel of strains, and also to test other target genes, to evaluate the usefulness of MLST for serogroup 1. MLST appears to be a promising typing technology, and by the selection of novel target genes this method has the potential of becoming the gold standard for population studies.

Citation: Gaia V, Peduzzi R. 2002. Multilocus Sequence Typing for Characterization of Serogroup 1 Isolates, p 251-253. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch47

Key Concept Ranking

Random Amplified Polymorphic DNA
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Pulsed-Field Gel Electrophoresis
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Figures

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FIGURE 1

Dendrogram of genetic relationships among 17 strains based on six gene fragments. ST and AFLP types are indicated at the right; genetic distance is indicated in the top scale.

Citation: Gaia V, Peduzzi R. 2002. Multilocus Sequence Typing for Characterization of Serogroup 1 Isolates, p 251-253. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch47
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References

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1. Enright M. C.,, and B. G. Spratt. 1998. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144:30493060.
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3. Fry, N. K.,, S. Alexiou-Daniel,, J. M. Bangsborg,, S. Bernander,, M. Castellani-Pastoris,, J. Etienne,, B. Forsblom,, V. Gaia,, J. H. Helbig,, D. Lindsay,, P. C. Lück,, C. Pelaz,, S. A. Uldum,, and T. J. Harrison. 1999. A multicenter evaluation of genotypic methods for epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin. Microbiol. Infect. 5:462477.
4. Fry, N. K.,, J. M. Bangsborg,, S. Bernander,, J. Etienne,, B. Forsblom,, V. Gaia,, P. Hasenberger,, D. Lindsay,, A. Papoutsi,, C. Pelaz,, M. Streuelens,, S. A. Uldum,, P. Visca,, and T. J. Harrison. 2000. Assessment of intercentre reproducibility and concordance of Legionella pneumophila serogroup 1 genotyping by amplified fragment length polymorphism analysis. Eur. J. Clin. Microbiol. Infect. Dis. 19:773780.
5. Maiden, M. C. J.,, J. A. Bygraves,, E. Feil,, G. Morelli,, J. E. Russell,, R. Urwin,, Q. Zhang,, J. Zhou,, K. Zurth,, D. A. Caugant,, I. M. Feavers,, M. Achtman,, and B. G. Spratt. 1998. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. USA 95:31403145.
6. Valsangiacomo, C.,, F. Baggi,, V. Gaia,, T. Balmelli,, R. Peduzzi,, and J. C. Piffaretti. 1995. Use of amplified fragment length polymorphism in molecular typing of Legionella pneumophila and application to epidemiological studies. J. Clin. Microbiol. 33:17161719.

Tables

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TABLE 1

MLST typing of 17 strains of serogroup 1

Citation: Gaia V, Peduzzi R. 2002. Multilocus Sequence Typing for Characterization of Serogroup 1 Isolates, p 251-253. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch47

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