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Chapter 48 : Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples

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Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples, Page 1 of 2

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Abstract:

is a naturally occurring aquatic bacterium found in lakes, rivers, hot springs, ponds, and moist soils. It causes Legionnaires' disease, a respiratory illness characterized by pneumonia. The disease is predominantly acquired by inhaling mist from contaminated water sources, and it is associated with considerable morbidity and mortality in immunocompromised hosts. Serological subtyping is insufficiently discriminatory when a given serogroup comprises only a few antigenically distinct subtypes, as for serogroup 6. Furthermore, genotypic differences have been reported in phenotypically similar organisms. Discriminatory molecular subtyping methods may be more informative to establish the environmental source of the illness. This chapter establishes and evaluates PCR and random amplified polymorphic DNA (RAPD) for detection and typing of from environmental sources. The authors' data show that RAPD analysis can be a better tool to discriminate between isolates compared with serotyping. Thus, it could be more informative in epidemiological studies to determine the source of infection.

Citation: Qasem J, Khan Z, Mustafa A. 2002. Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples, p 254-256. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch48

Key Concept Ranking

Random Amplified Polymorphic DNA
0.54079694
Legionella pneumophila
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Figures

Image of FIGURE 1
FIGURE 1

RAPD patterns of environmental isolates of serogroup 1. Lanes marked 25, 27, 28, 29, 32, 33, 41, and 24 are RAPD patterns of respective isolates. Lanes marked kb are 100-bp molecular size markers.

Citation: Qasem J, Khan Z, Mustafa A. 2002. Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples, p 254-256. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch48
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Image of FIGURE 2
FIGURE 2

RAPD bands of 34 environmental isolates of belonging to serogroup 3. Lanes 1 through 42 are RAPD patterns of respective isolates, kb, molecular weight markers (100-bp DNA ladder).

Citation: Qasem J, Khan Z, Mustafa A. 2002. Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples, p 254-256. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch48
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Download as Powerpoint

References

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1. Brown, A.,, V. L. Yu,, M. H. Magnussen,, G. M. Vickers,, R. M. Garrity,, and E. M. Elder. 1982. Isolation of Pittsburgh pneumonia agent from a hospital shower. Appl. Environ. Microbiol. 43:725726.
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14. Visca, P.,, P. Goldoni,, P. C. Luck,, J. H. Helbig,, L. Cattani,, G. Giltri,, S. Bramati,, and M. Castellani Pastoris. 1999. Multiple types of Legionella pneumophila serogroup 6 in a hospital heated-water system associated with sporadic infections. J. Clin. Microbiol 37:21892196.
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Tables

Generic image for table
TABLE 1

Comparison of culture, genus-specific, and species-specific PCR for detection of in environmental samples

Citation: Qasem J, Khan Z, Mustafa A. 2002. Evaluation of PCR and Random Amplification of Polymorphic DNA for Detection and Typing of in Environmental Water Samples, p 254-256. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch48

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