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Chapter 52 : Typing of Strains Isolated from Patients and Environmental Sources in Germany, 1999–2000

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Typing of Strains Isolated from Patients and Environmental Sources in Germany, 1999–2000, Page 1 of 2

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Abstract:

spp. are gram-negative bacteria responsible for epidemic and sporadic cases of pneumonia after inhalation of contaminated water droplets from a variety of water sources. This chapter presents the distribution of species, serogroups, and monoclonal subtypes of strains isolated in Germany and typed at the National Reference Laboratory for . Environmental strains were isolated on selective buffered charcoal-yeast extract agar from water samples collected at various locations in Germany. The monoclonal subtypes were named according to the reactivity with reference strains. For macrorestriction analysis, chromosomal DNAs were digested overnight with I, I, and l (New England Biolabs, Schwahlbach, Germany) and separated with the CHEF III System (BioRad Laboratories, Munich, Germany). In this study, all patients had a radiographically confirmed pneumonia confirmed as legionellosis by isolating a strain from clinical specimens. Cases were classified as nosocomial if the patient had been hospitalized continuously for a minimum of 3 days at the time of disease onset or had been discharged from the hospital less than 10 days before the onset of symptoms. Of the 133 strains grown from clinical samples, 88 (66%) belonged to serogroup 1, 38 (29%) belonged to serogroups 2 to 15, and 7 (5%) were typed as other species. These data are in good agreement with results from Europe and the United States.

Citation: Lück P, Helbig J. 2002. Typing of Strains Isolated from Patients and Environmental Sources in Germany, 1999–2000, p 267-270. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch52

Key Concept Ranking

Gram-Negative Bacteria
0.6293871
Chromosomal DNA
0.50643384
Legionella
0.45154488
Sequence Analysis
0.44740486
0.6293871
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References

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1. Benson, R. F.,, and B. S. Fields. 1998. Classification of the genus Legionella. Semin. Respir. Infect. 13:9099.
2. Breiman, R. F.,, and J. C. Butler. 1998. Legionnaires' disease: clinical, epidemiological, and public health perspectives. Semin. Respir. Infect. 13:8489.
3. Dournon, E.,, W. F. Bibb,, P. Rjagopalan,, N. Desplaces,, and R. M. McKinney. 1988. Monoclonal antibody reactivity as a virulence marker for Legionella pneumophila serogroup 1 strains. J. Infect. Dis. 157:496501.
4. European Working Group on Legionella Infections. 1999. Legionnaires disease, Europe 1997. Wkly. Epidemiol. Rec. 73:257264.
5. European Working Groupon Legionella Infections. 1998. Legionnaires disease, Europe 1998. Wkly. Epidemiol. Rec. 74:273280.
6. Helbig, J. H.,, J. B. Kurtz,, M. Castellani Pastoris,, C. Pelaz,, and P. C. Luck. 1997. Antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species and serogroups. J. Clin. Miaobiol. 35: 28412845.
7. Joly, J. R.,, R. M. McKinney,, J. H. Tobin,, W. F. Bibb,, I. D. Watkins,, and D. Ramsay. 1986. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23:768771.
8. Joly, J. R.,, Y. Y. Chen,, and D. Ramsay. 1993. Serogrouping and subtyping of Legionella pneumophila with monoclonal antibodies. J. Clin. Miaobiol. 18:10401046.
9. Lück, P. C.,, I. Leupold,, M. Hlawitschka,, J. H. Helbig,, I. Carmienke,, L. Jatzwauk,, and T. Guderitz. 1993. Prevalence of Legionella species, serogroups, and monoclonal subgroups in hot water systems in south-eastern Germany. Zentralbl. Hyg. 193:450460.
10. Lück, P. C.,, H. M. Wenchel,, and J. H. Helbig. 1998. Nosocomial pneumonia caused by three genetically different strains of Legionella pneumophila and detection of these strains in the hospital water supply. J. Clin. Miaobiol. 36:11601163.
11. Marston, B. J.,, H. B. Lippman,, and R. F. Breiman. 1994. Surveillance for Legionnaires' disease. Arch. Mem. Med. 154:24172422.
12. Ratcliflf, R. M.,, J. A. Lanser,, P. A. Manning,, and M. W. Heuzenroeder. 1998. Sequence-based classification scheme for the genus Legionella targeting the mip gene. J. Clin. Microbiol. 36:15601567.
13. Slaymaker, E.,, C. A. Joseph, and, C. L. R. Bartlett on behalf of the Europe an Working Groupon Legionella Infections. 1999. Travel associated legionnaires' disease in Europe 1997 and 1998. Eurosurveillance 4:120124.
14. Winn, W. C., 1995. Legionella, p. 533544. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, D.C.

Tables

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TABLE 1

Distribution of Legionella species, serogroups, and monoclonal subgroups among clinical and environmental isolates

Citation: Lück P, Helbig J. 2002. Typing of Strains Isolated from Patients and Environmental Sources in Germany, 1999–2000, p 267-270. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch52

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