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Chapter 53 : Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens

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Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens, Page 1 of 2

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Abstract:

The isolation of species from environmental specimens has multiple steps subject to many potential errors, which have a cumulative effect. The growth medium is relatively complex and contains heat-sensitive ingredients, and the preparation involves multiple aseptic additions after autoclaving. The selective media and pretreatments do not suppress all background flora, and the recognition of colonies of , particularly non- species, requires experience. In addition background bacterial flora can sometimes suppress the growth of colonies of completely. It is important that all laboratories in different countries produce equivalent reliable results. To achieve this, each laboratory needs a comprehensive quality assurance program including good internal quality control and participation in external quality assurance (EQA) schemes. The beneficial effects of belonging to an EQA scheme have been demonstrated by the gradual improvement in the consistency of the laboratories. This in turn is leading to more reliable quantification, which is important for monitoring the effectiveness of control measures throughout the world.

Citation: Lee J, Surman S, Hall M, Cuthbert L. 2002. Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens, p 271-274. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch53

Key Concept Ranking

Legionella pneumophila
0.8055556
Selective Media
0.5290609
0.8055556
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Figures

Image of FIGURE 1
FIGURE 1

Distribution of counts of reported by participants from direct inoculation of sample C from distribution L 29, April 2000. The numbers above each bar are the number of non-United Kingdom laboratories included within the group represented by the bar.

Citation: Lee J, Surman S, Hall M, Cuthbert L. 2002. Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens, p 271-274. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch53
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Image of FIGURE 2
FIGURE 2

Distribution of counts of reported by participants after diluting and processing sample C from distribution L 29, April 2000. The numbers above each bar are the number of non-United Kingdom laboratories included within the group represented by the bar. UC, uncountable.

Citation: Lee J, Surman S, Hall M, Cuthbert L. 2002. Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens, p 271-274. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch53
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Download as Powerpoint

References

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Tables

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TABLE 1

Proportion of laboratories detecting species other than

Citation: Lee J, Surman S, Hall M, Cuthbert L. 2002. Development of an International External Quality Assurance Scheme for Isolation of Species from Environmental Specimens, p 271-274. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch53

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