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Chapter 55 : Proposed Method for Optimum Recovery of from Cooling Water in South Africa

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Proposed Method for Optimum Recovery of from Cooling Water in South Africa, Page 1 of 2

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Abstract:

This chapter evaluates the efficacy of international detection methods compared with an in house-developed method for the optimum recovery of from cooling water, determines the effect of heat and acid pretreatment procedures as well as the use of selective supplement on recovery, investigates possible antibody cross-reactions with nonlegionellae using a polyvalent direct fluorescent antibody conjugate specific for species known to cause most outbreaks of legionellosis worldwide, and formulates guidelines to assist industry in the management and control of levels in cooling water. The results of a preliminary study showed that in 50 spiked tap, source, and cooling water samples, acid treatment had the most damaging effect on the recovery of from tap water, followed by source water and then cooling water. Heat-treated spiked cooling water gave poor recovery rates, followed by source water and tap water. The most probable number (MPN) method is proposed as the national standard procedure for the detection of from cooling water in South Africa. In the case of the ISO method, all representative colonies need to be confirmed using blood agar, buffered charcoal-yeast extract (BCYE) (without cysteine), latex agglutination, and/or direct fluorescent antibodies.

Citation: Coubrough P, Modisenyane T, Genthe B. 2002. Proposed Method for Optimum Recovery of from Cooling Water in South Africa, p 280-283. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch55
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FIGURE 1

Methods of detection and treatment procedures. Abbreviations: NT, no treatment; AT, acid treatment; HT( ), heat treatment (AS: BCYE + MWY); HT( ), heat treatment (AS: BCYE + BMPA); MPN, most probable number; ISO, international standard; AS, Australian standard.

Citation: Coubrough P, Modisenyane T, Genthe B. 2002. Proposed Method for Optimum Recovery of from Cooling Water in South Africa, p 280-283. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch55
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References

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1. Grabow, N. A.,, R. Kfir,, and W. O. K. Grabow. 1991. A most probable number method on the enumeration of Legionella bacteria in water. Water Set. Technol. 24:143147.
2. International Standards Organization. 1998. Report: International Standard: Water quality—Detection and Enumeration of Legionella, ISO 11731, p. 116.
3. Australian Standard. 1991. Report: Australian Standard: Water—Examination for Legionellae, AS 3896.

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