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Chapter 60 : Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies

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Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, Page 1 of 2

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Abstract:

serogroup 1 is the serogroup of species most frequently isolated from infected patients in Japan and all over the world. The recent findings suggest that one of the most discriminative epidemiological methods is pulsed-field gel electrophoresis (PFGE). To develop the method of epidemiological surveillance, PFGE and monoclonal antibodies were used for the analysis of 27 clinical and 20 environmental serogroup 1 isolates in Japan. Researchers reported five anti- serogroup 1 monoclonal antibodies that were specific for serogroup 1 but showed different reactivities, previously. Monoclonal antibody-sensitized latex was prepared and the latex agglutination test on slide plate was performed. There was a close correlation between monoclonal antibody subtyping and PFGE analysis. The result by the two analyses suggested that the clinical isolates, the isolates from cooling towers, and the isolates from hot springs in Japan formed the distinctive genetically or antigenic clusters.

Citation: Amemura-Maekawa J, Kura F, Watanabe H, Gondaira F, Sugiyama J. 2002. Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, p 302-304. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch60

Key Concept Ranking

Pulsed-Field Gel Electrophoresis
0.6759961
Latex Agglutination Test
0.60839653
Legionella pneumophila
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Figures

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FIGURE 1

Cluster dendrogram of Japanese L. pneumophila serogroup 1 isolates generated from Sfil restriction fragments separated by PFGE and analyzed with the program Molecular Analyst (Bio-Rad). Designations of strains were indicated along with original ones by providers. Source is as follows: E, environmental; HS, hot spring; CT, cooling tower; C, clinical.

Citation: Amemura-Maekawa J, Kura F, Watanabe H, Gondaira F, Sugiyama J. 2002. Analysis of Serogroup 1 Isolates in Japan by Using Pulsed-Field Gel Electrophoresis and Monoclonal Antibodies, p 302-304. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch60
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References

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