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Chapter 75 : Epidemiological Typing of pneumophtla Serogroup 5 Strains

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Epidemiological Typing of pneumophtla Serogroup 5 Strains, Page 1 of 2

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Abstract:

Following the discovery of as the etiological agent of Legionnaires' disease, many different serogroups and related species of this bacterium have been detected. A newly developed PCR method was used to generate strain-specific DNA fingerprinting profiles. This method is compared with the results of typing the same isolates with a set of monoclonal antibodies (MAbs). To identify different strains of , the authors used different primers to amplify DNA fragments in crude bacterial lysates to generate banding profiles. The complete Dresden MAb panel contains 98 MAbs related to lipopolysaccharide characteristics. Testing of the isolates was made with these MAbs utilized in the enzyme-linked immunosorbent assay method. The authors conclude that a combination of Göttingen direct fluorescent antibody technique and PCR methods can be a useful tool for subtyping serogroup 5 strains.

Citation: Zietz B, Wiese J, Dunkelberg H, Lück P, Helbig J. 2002. Epidemiological Typing of pneumophtla Serogroup 5 Strains, p 364-368. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch75

Key Concept Ranking

Fluorescent Antibody Technique
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Enzyme-Linked Immunosorbent Assay
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DNA Fingerprinting
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0.5613192
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Figures

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FIGURE 1

PCR products of serogroup 5 isolates tested with primer Eric2 and a combination of Lpm-1 + Lpm-2: (lanes left to right, fit together from separate gels) molecular weight marker (Μ), Ζ 79 (lane 1), Ζ 132b, Ζ 290, WE 02 XI 1, KS E4 XII 1 ( ), WL E4 XV 2, Va03335, Gö-26, Gö-215, Gö-262 (10), ATCC 33216, ATCC 33737, Denmark 6, Denmark 9, Italy 31 (15), Italy 43, Scotland 6, RingL 12A, WL 702, WL 582-4 (20), molecular weight marker (M).

Citation: Zietz B, Wiese J, Dunkelberg H, Lück P, Helbig J. 2002. Epidemiological Typing of pneumophtla Serogroup 5 Strains, p 364-368. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch75
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References

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1. Benson, R. F.,, and B. S. Fields. 1998. Classification of the genus Legionella. Semin, Respir. Infect. 13:9099.
2. Chang, F. Y.,, S. L. Jacobs,, S. M. Colodny,, J. E. Stout,, and V. L. Yu. 1996. Nosocomial Legionnaires' disease caused by Legionella pneumophila serogroup 5: laboratory and epidemiologic implications. J. Infect. Dis. 174:11161119.
3. Helbig, J. H.,, J. B. Kurtz,, M. C. Pastoris,, C. Pelaz,, and P. C. Luck. 1997. Antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups. J. Clin. Microbiol. 35:28412845.
4. Jaulhac, B.,, M. Nowicki,, N. Bornstein,, O. Meunier,, G. Prevost,, Y. Piemont,, J. Fleurette,, and H. Monteil. 1992. Detection of Legionella spp. in bronchoalveolar lavage fluids by DNA amplification. J. Clin. Microbiol. 30:920924.
5. van Belkum, A.,, M. Struelens,, and W. Quint. 1993. Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting. J. Clin. Microbiol. 31:21982200.
6. Zietz, B.,, J. Wiese,, F. Brengelmann,, and H. Dunkelberg. 2001. Presence of Legionellaceae in warm water supplies and typing of strains by polymerase chain reaction. Epidemiol. Infect. 126: 147152.

Tables

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TABLE 1

Results of typing serogroup 5 strains

Citation: Zietz B, Wiese J, Dunkelberg H, Lück P, Helbig J. 2002. Epidemiological Typing of pneumophtla Serogroup 5 Strains, p 364-368. In Marre R, Abu Kwaik Y, Bartlett C, Cianciotto N, Fields B, Frosch M, Hacker J, Lück P (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555817985.ch75

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