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Chapter 19 : Protein Export

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Abstract:

This chapter describes what is known about protein export in from the perspective of the general mechanisms for protein export in gram-negative bacteria. Autotransporters constitute one terminal branch of the general secretory pathway (GSP), frequently employed to export virulence factors. A new Sec-independent system for translocation of proteins across the inner membrane (IM) has recently been identified, and orthologs of the essential genes of this system have been found in the genome. Sec-dependent translocation across the IM requires the substrate protein to be unfolded. Most ATP-binding cassette (ABC) transporters are, however, not involved in protein export, but in the efflux and influx of a diverse group of substrates. To date, none of the potential ABC transporters in has been proposed to be involved in protein export. Motility is mediated by the sheathed flagella in and is necessary for colonization in animal models. In the context of the chapter the flagellar export apparatus potentially is interesting owing to its similarity to type III export systems. Recently, in a different system, it has been demonstrated that the flagellar transport apparatus of also functions as a protein export system for a pathogenesis-related phospholipase, placing the flagellar export/assembly system under a new light. The proteins discussed in a section, urease, heat shock proteins, and superoxide dismutase, are found only in the cytoplasm of most bacterial species.

Citation: Ilver D, Rappoulli R, Telford J. 2001. Protein Export, p 219-226. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch19
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1. Akopyants, N. S.,, S. W. Clifton,, D. Kersulyte,, J. E. Crabtree,, B. E. Youree,, C. A. Reece,, N. O. Bukanov,, E. S. Drazek,, B. A. Roe,, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol. Microbiol. 28: 37 53.
2. Alm, R. A.,, L. S. Ling,, D. T. Molr,, B. L. King,, E. D. Brown,, P. C. Doig,, D. R. Smith,, B. Noonan,, B. C. Guild,, B. L. deJonge,, G. Carmel,, P. J. Tummino,, A. Caruso,, M. Uria-Nickelsen,, D. M. Mills,, C. Ives,, R. Gibson,, D. Merberg,, S. D. Mills,, Q. Jiang,, D. E. Taylor,, G. F. Vovis,, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397: 176 180. ( Erratum, 397:719.)
3. Asahi, M.,, T. Azuma,, S. Ito,, Y. Ito,, H. Suto,, Y. Nagai,, M. Tsubokawa,, Y. Tohyama,, S. Maeda,, M. Omata,, T. Suzuki,, and C. Sasakawa. 2000. Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. J. Exp. Med. 191: 593 602.
4. Berry, A. M.,, J. C. Paton,, and D. Hansman. 1992. Effect of insertional inactivation of the genes encoding pneumolysin and autolysin on the virulence of Streptococcus pneumoniae type 3. Microb. Pathog. 12: 87 93.
5. Beveridge, T. J. 1999. Structures of gram-negative cell walls and their derived membrane vesicles. J. Bacteriol. 181: 4725 4733.
6. Binet, R.,, S. Letoffe,, J. M. Ghigo,, P. Delepelaire,, and C. Wandersman. 1997. Protein secretion by gram-negative bacterial ABC exporters—a review. Gene 192: 7 11.
7. Blaser, M. J.,, and J. Parsonnet. 1994. Parasitism by the "slow" bacterium Helicobacter pylori leads to altered gastric homeostasis and neoplasia. J. Clin. Invest. 94: 4 8.
9. Censini, S.,, C. Lange,, Z. Xiang,, J. E. Crabtree,, P. Ghiara,, M. Borodovsky,, R. Rappuoli,, and A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA 93: 14648 14653.
10. Christie, P. J.,, and J. P. Vogel. 2000. Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells. Trends Microbiol. 8: 354 360.
11. Covacci, A.,, S. Censini,, M. Bugnoli,, R. Petracca,, D. Burroni,, G. Macchia,, A. Massone,, E. Papini,, Z. Xiang,, N. Figura,, and R. Rappuoli. 1993. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA 90: 5791 5795.
12. Cover, T. L.,, M. K. Tummuru,, P. Cao,, S. A. Thompson,, and M. J. Blaser. 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269: 10566 10573.
13. Dalbey, R. E.,, and C. Robinson. 1999. Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane. Trends Biochem. Sci. 24: 17 22.
14. Dunn, B. E.,, N. B. Vakil,, B. G. Schneider,, M. M. Miller,, J. B. Zitzer,, T. Peutz,, and S. H. Phadnis. 1997. Localization of Helicobacter pylori urease and heat shock protein in human gastric biopsies. Infect. Immun. 65: 1181 1188.
15. Eaton, K. A.,, C. L. Brooks,, D. R. Morgan,, and S. Krakowka. 1991. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect. Immun. 59: 2470 2475.
16. Eaton, K. A.,, and S. Krakowka. 1994. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect. Immun. 62: 3604 3607.
17. Eaton, K. A.,, S. Suerbaum,, C. Josenhans,, and S. Krakowka. 1996. Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes. Infect. Immun. 64: 2445 2448.
18. Economou, A. 1999. Following the leader: bacterial protein export through the Sec pathway. Trends Microbiol. 7: 315 320.
19. Eschweiler, B.,, B. Bohrmann,, B. Gerstenecker,, E. Schiltz,, and M. Kist. 1993. In situ localization of the 60 k protein of Helicobacter pylori, which belongs to the family of heat shock proteins, by immuno-electron microscopy. Zentralbl. Bakteriol. 280: 73 85.
20. Fiocca, R.,, V. Necchi,, P. Sommi,, V. Ricci,, J. Telford,, T. L. Cover,, and E. Solcia. 1999. Release of Helicobacter pylori vacuolating cytotoxin by both a specific secretion pathway and budding of outer membrane vesicles. Uptake of released toxin and vesicles by gastric epithelium. J. Pathol. 188: 220 226.
21. Fisher, W.,, and R. Haas. Personal communication.
22. Geis, G.,, H. Leying,, S. Suerbaum,, U. Mai,, and W. Opferkuch. 1989. Ultrastructure and chemical analysis of Campylobacter pylori flagella. J. Clin. Microbiol. 27: 436 441.
23. Hawtin, P. R.,, A. R. Stacey,, and D. G. Newell. 1990. Investigation of the structure and localization of the urease of Helicobacter pylori using monoclonal antibodies. J. Gen. Microbiol. 136( Pt 10): 1995 2000.
24. Horstman, A. L.,, and M. J. Kuehn. 2000. Enterotoxigenic Escherichia coli secretes active heat-labile enterotoxin via outer membrane vesicles. J. Biol. Chem. 275: 12489 12496.
25. Hueck, C. J. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol. Mol. Biol. Rev. 62: 379 433.
26. Jenks, P. J.,, S. Foynes,, S. J. Ward,, C. Constantinidou,, C. W. Penn,, and B. W. Wren. 1997. A flagellar-specific ATPase (Flil) is necessary for flagellar export in Helicobacter pylori. FEMS Microbiol. Lett. 152: 205 211.
27. Jonsson, A. B.,, G. Nyberg,, and S. Normark. 1991. Phase variation of gonococcal pili by frameshift mutation in pilC, a novel gene for pilus assembly. EMBO J. 10: 477 488.
28. Kadurugamuwa, J. L.,, and T. J. Beveridge. 1996. Bacteriolytic effect of membrane vesicles from Pseudomonas aeruginosa on other bacteria including pathogens: conceptually new antibiotics. J. Bacteriol. 178: 2767 2774.
29. Kadurugamuwa, J. L.,, and T. J. Beveridge. 1998. Delivery of the non-membrane-permeative antibiotic gentamicin into mammalian cells by using Shigella flexneri membrane vesicles. Antimicrob. Agents Chemother. 42: 1476 1483.
30. Kadurugamuwa, J. L.,, and T. J. Beveridge. 1995. Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion. J. Bacteriol. 177: 3998 4008.
31. Keenan, J.,, T. Day,, S. Neal,, B. Cook,, G. Perez-Perez,, R. Allardyce,, and P. Bagshaw. 2000. A role for the bacterial outer membrane in the pathogenesis of Helicobacter pylori infection. FEMS Microbiol. Lett. 182: 259 264.
32. Klauser, T.,, J. Pohlner,, and T. F. Meyer. 1990. Extracellular transport of cholera toxin B subunit using Neisseria IgA protease beta-domain: conformation-dependent outer membrane translocation. EMBO J. 9: 1991 1999.
33. Krishnamurthy, P.,, M. Parlow,, J. B. Zitzer,, N. B. Vakil,, H. L. Mobley,, M. Levy,, S. H. Phadnis,, and B. E. Dunn. 1998. Helicobacter pylori containing only cytoplasmic urease is susceptible to acid. Infect. Immun. 66: 5060 5066.
34. Kubori, T.,, Y. Matsushima,, D. Nakamura,, J. Uralil,, M. Lara-Tejero,, A. Sukhan,, J. E. Galan,, and S. I. Aizawa. 1998. Supra-molecular structure of the Salmonella typhimurium type III protein secretion system. Science 280: 602 605.
35. Leying, H.,, S. Suerbaum,, G. Geis,, and R. Haas. 1992. Cloning and genetic characterization of a Helicobacter pylori flagellin gene. Mol. Microbiol. 6: 2863 2874.
36. Maurer, J.,, J. Jose,, and T. F. Meyer. 1999. Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions. J. Bacteriol. 181: 7014 7020.
37. Nguyen, L.,, I. T. Paulsen,, J. Tchieu,, C. J. Hueck,, and M. H. Saier, Jr. 2000. Phylogenetic analyses of the constituents of type III protein secretion systems. J. Mol. Microbiol Biotechnol. 2: 125 144.
38. O'Toole, P. W.,, M. Kostrzynska,, and T. J. Trust. 1994. Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production. Mol. Microbiol. 14: 691 703.
39. Odenbreit, S.,, J. Puis,, B. Sedlmaier,, E. Gerland,, W. Fischer,, and R. Haas. 2000. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 287: 1497 1500.
40. Paton, J. C.,, P. W. Andrew,, G. J. Boulnois,, and T. J. Mitchell. 1993. Molecular analysis of the pathogenicity of Streptococcus pneumoniae: the role of pneumococcal proteins. Annu. Rev. Microbiol. 47: 89 115.
41. Paulsen, I. T.,, L. Nguyen,, M. K. Sliwinski,, R. Rabus,, and M. H. Saier, Jr. 2000. Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes. J. Mol. Biol. 301: 75 100.
42. Phadnis, S. H.,, D. liver,, L. Janzon,, S. Normark,, and T. U. Westblom. 1994. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect. Immun. 62: 1557 1565.
43. Phadnis, S. H.,, M. H. Parlow,, M. Levy,, D. liver,, C. M. Caulkins,, J. B. Connors,, and B. E. Dunn. 1996. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. Infect. Immun. 64: 905 912.
44. Pohlner, J.,, R. Halter,, K. Beyreuther,, and T. F. Meyer. 1987. Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease. Nature 325: 458 462.
45. Porwollik, S.,, B. Noonan,, and P. W. O'Toole. 1999. Molecular characterization of a flagellar export locus of Helicobacter pylori. Infect. Immun. 67: 2060 2070.
46. Russel, M. 1998. Macromolecular assembly and secretion across the bacterial cell envelope: type II protein secretion systems. J. Mol. Biol. 279: 485 499.
47. Santini, C. L.,, B. Ize,, A. Chanal,, M. Muller,, G. Giordano,, and L. F. Wu. 1998. A novel sec-independent periplasmic protein translocation pathway in Escherichia coli. EMBO J. 17: 101 112.
48. Sargent, F.,, N. R. Stanley,, B. C. Berks,, and T. Palmer. 1999. Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein. J. Biol. Chem. 274: 36073 36082.
49. Schmitt, W.,, and R. Haas. 1994. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol. 12: 307 319.
50. Schraw, W.,, M. S. McClain,, and T. L. Cover. 1999. Kinetics and mechanisms of extracellular protein release by Helicobacter pylori. Infect. Immun. 67: 5247 5252.
51. Segal, E. D.,, J. Cha,, J. Lo,, S. Falkow,, and L. S. Tompkins. 1999. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 96: 14559 14564.
52. Segal, E. D.,, C. Lange,, A. Covacci,, L. S. Tompkins,, and S. Falkow. 1997. Induction of host signal transduction pathways by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 94: 7595 7599.
53. Seifert, H. S.,, R. S. Ajioka,, C. Marchal,, P. F. Sparling,, and M. So. 1988. DNA transformation leads to pilin antigenic variation in Neisseria gonorrhoeae. Nature 336: 392 395.
54. Settles, A. M.,, and R. Martienssen. 1998. Old and new pathways of protein export in chloroplasts and bacteria. Trends Cell. Biol. 8: 494 501.
55. Sommi, P.,, V. Ricci,, R. Fiocca,, V. Necchi,, M. Romano,, J. L. Telford,, E. Solcia,, and U. Ventura. 1998. Persistence of Helicobacter pylori VacA toxin and vacuolating potential in cultured gastric epithelial cells. Am. J. Physiol. 275( 4 Pt 1): G681 G688.
56. Spiegelhalder, C.,, B. Gerstenecker,, A. Kersten,, E. Schiltz,, and M. Kist. 1993. Purification of Helicobacter pylori superoxide dismutase and cloning and sequencing of the gene. Infect. Immun. 61: 5315 5325.
57. Stein, M.,, R. Rappuoli,, and A. Covacci. 2000. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc. Natl. Acad. Sci. USA 97: 1263 1268.
58. Suzuki, T.,, M. C. Lett,, and C. Sasakawa. 1995. Extracellular transport of VirG protein in Shigella. J. Biol. Chem. 270: 30874 30880.
59. Telford, J. L.,, P. Ghiara,, M. Dell'Orco,, M. Comanducci,, D. Burroni,, M. Bugnoli,, M. F. Tecce,, S. Censini,, A. Covacci,, Z. Xiang,, E. Papini,, C. Montecucco,, L. Parente,, and R. Rappuoli. 1994. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med. 179: 1653 1658.
60. Thanassi, D. G.,, E. T. Saulino,, and S.J. Hultgren. 1998. The chaperone/usher pathway: a major terminal branch of the general secretory pathway. Curr. Opin. Microbiol. 1: 223 231.
61. The Institute for Genomic Research, http://www.tigr.org/.
62. Tomb, J.-F.,, O. White,, A. R. Kerlavage,, R. A. Clayton,, G. G. Sutton,, R. D. Fleischmann,, K. A. Ketchum,, H. P. Klenk,, S. Gill,, B. A. Dougherty,, K. Nelson,, J. Quackenbush,, L. Zhou,, E. F. Kirkness,, S. Peterson,, B. Loftus,, D. Richardson,, R. Dodson,, H. G. Khalak,, A. Glodek,, K. McKenney,, L. M. Fitzgerald,, N. Lee,, M. D. Adams,, E. K. Hickey,, D. E. Berg,, J. D. Gocayne,, T. R. Utterback,, J. D. Peterson,, J. M. Kelley,, M. D. Cotton,, J. M. Weidman,, C. Fujii,, C. Bowman,, L. Watthey,, E. Wallin,, W. S. Hayes,, M. Borodovsky,, P. D. Karp,, H. O. Smith,, C. M. Fraser,, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539 547. ( Erratum, 389:412.)
63. Vanet, A.,, and A. Labigne. 1998. Evidence for specific secretion rather than autolysis in the release of some Helicobacter pylori proteins. Infect. Immun. 66: 1023 1027.
64. Veiga, E.,, V. de Lorenzo,, and L. A. Fernandez. 1999. Probing secretion and translocation of a beta-autotransporter using a reporter single-chain Fv as a cognate passenger domain. Mol. Microbiol. 33: 1232 1243.
65. Xiang, Z.,, S. Censini,, P. F. Bayeli,, J. L. Telford,, N. Figura,, R. Rappuoli,, and A. Covacci. 1995. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect. Immun. 63: 94 98.
66. Young, G. M.,, D. H. Schmiel,, and V. L. Miller. 1999. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc. Natl. Acad. Sci. USA 96: 6456 6461.

Tables

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Table 1

Summary of protein export in

Citation: Ilver D, Rappoulli R, Telford J. 2001. Protein Export, p 219-226. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch19

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