Chapter 26 : Transcription and Translation

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Regulation of the transcription and translation mechanisms of is presumed to be similar to those of the well-studied , because both are gram-negative bacteria. Complete DNA sequencing of the genomes of two different strains of has allowed the application of powerful sequence homology searches to assign putative functions to various open reading frames (ORFs), leading to hypotheses regarding transcriptional and translational processes in genomes. This chapter focuses on experimental results dealing with transcription and translation obtained in the past few years. DNA-dependent RNA polymerase mediates synthesis of RNA from a DNA template. The discovery that undergoes spontaneous autolysis toward the end of log-phase growth and the conversion of bacteria into coccoid forms is consistent with these explanations. The lack of an identifiable ortholog of has been interpreted as suggesting that may respond to stress differently than other bacteria. promoters are classified by the sigma factors involved (or presumed to be involved) to direct their transcription, i.e., the σ-dependent, σ-dependent, and σ-dependent promoters. The translational apparatus is required for expression of the genetic information encoded in cells. Components include ribosomes, tRNA, mRNA, numerous ligands, ions, nucleotides, and several proteins, including tRNA-modifying enzymes, aminoacyl- tRNA synthetases, and the proteins transiently associated with ribosomes. The genome is unique in the absence of an asparaginyl-tRNA synthetase gene. Complete DNA sequencing of the genome has allowed sequence-similarity comparisons with other gram-negative bacteria.

Citation: Bhattacharyya S, Dunn B, Phadnis S, Go M. 2001. Transcription and Translation, p 285-291. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch26

Key Concept Ranking

Gene Expression and Regulation
RNA Polymerase
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Table 1

Known properties of various promoters

Citation: Bhattacharyya S, Dunn B, Phadnis S, Go M. 2001. Transcription and Translation, p 285-291. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch26
Generic image for table
Table 2

Putative tRNA-modifying enzymes, related genes, and ORFs

Citation: Bhattacharyya S, Dunn B, Phadnis S, Go M. 2001. Transcription and Translation, p 285-291. In Mobley H, Mendz G, Hazell S (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555818005.ch26

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