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Chapter 14 : Lyme Borreliosis

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Lyme Borreliosis, Page 1 of 2

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Abstract:

Lyme borreliosis (Lyme disease) is unequivocally a persistent infectious disease. , the agent of Lyme disease, persists in both humans and animals, despite a vigorous and effective immune response by the infected host. The agent of Lyme disease also owes its survival to its propensity to infect a wide variety of mammalian and avian hosts, thereby allowing geographic spread and adaptation to new niches. The majority (60 to 80%) of patients with Lyme disease develop an expanding annular skin lesion (erythema migrans) at the site of the tick bite. Borrelia plasmids are fundamentally different from plasmids of other bacteria in that they contain essential, rather than nonessential, extrachromosomal elements of the genome. The phenomenon of lateral gene transfer and recombination may have selective advantages in mixed populations of spirochetes but does not provide an explanation for the immune evasion of clonal populations of spirochetes during persistent infection. The proof of the pudding for antigenic variation is whether antigenic changes in spirochetes provide selective advantages for the variant subpopulation in evading immune clearance. No evidence for this phenomenon has been observed with . Analysis of the outer and inner membranes of spirochetes indicates that the majority of protein is associated with the inner membrane and that proteins associated with the outer membrane, including OspC, may have only limited surface exposure.

Citation: Barthold S. 2000. Lyme Borreliosis, p 281-304. In Nataro J, Blaser M, Cunningham-Rundles S (ed), Persistent Bacterial Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818104.ch14

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Enzyme-Linked Immunosorbent Assay
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Outer Membrane Proteins
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Human Lyme Disease
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Immune Systems
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Figures

Image of FIGURE 1
FIGURE 1

Indirect immunohistochemistry of joint tissue from an infected SCID mouse probed with immune serum from a i-infected immunocompetent mouse. Immune serum contains antibodies that are reactive to spirochetes in joint tissue, but the target antigen(s) has not been implicated.

Citation: Barthold S. 2000. Lyme Borreliosis, p 281-304. In Nataro J, Blaser M, Cunningham-Rundles S (ed), Persistent Bacterial Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818104.ch14
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Image of FIGURE 2
FIGURE 2

Indirect immunofluorescence of the midgut of a flat (resting) tick labeled with antiserum to OspA. Spirochetes within the midgut of the tick are strongly reactive with OspA antibody. (Reprinted from reference .)

Citation: Barthold S. 2000. Lyme Borreliosis, p 281-304. In Nataro J, Blaser M, Cunningham-Rundles S (ed), Persistent Bacterial Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818104.ch14
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Image of FIGURE 3
FIGURE 3

Indirect immunofluorescence of the midgut of a tick that has fed upon a mouse immunized with OspA. Ingestion of OspA antibody has killed organisms, with no viable spirochetes remaining in the midgut. (Reprinted from reference .)

Citation: Barthold S. 2000. Lyme Borreliosis, p 281-304. In Nataro J, Blaser M, Cunningham-Rundles S (ed), Persistent Bacterial Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818104.ch14
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Image of FIGURE 4
FIGURE 4

Indirect immunofluorescence and confocal microscopy of a tick salivary gland following onset of feeding, demonstrating loss of OspA following migration to salivary glands during feeding. Spirochetes are labeled with antiserum to (A) but are not labeled with antiserum to OspA (B). (With permission from E. Fikrig.)

Citation: Barthold S. 2000. Lyme Borreliosis, p 281-304. In Nataro J, Blaser M, Cunningham-Rundles S (ed), Persistent Bacterial Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818104.ch14
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References

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1. Aguero-Rosenfeld , M. E.,, J. Nowakowski,, D. F. McKenna,, C. A. Carbonaro,, and G. P. Wormser. 1993. Serodiagnosis in early Lyme disease. J. Clin. Microbiol. 31:30903095.
2. Akins, D. R.,, S. F. Porcella,, T. G. Popova,, D. Shevchenko,, S. I. Baker,, M. Li,, M. V. Norgard,, and J. D. Radolf. 1995. Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologue. Mol. Microbiol. 18:507520.
3. Anderson, J. F.,, and L. A. Magnarelli. 1993. Epizootiology of Lyme disease-causing borreliae. Clin. Dermatol. 11:339351.
4. Armstrong, A. L.,, S. W. Barthold,, D. H. Persing,, and D. S. Beck. 1992. Carditis in Lyme disease susceptible and resistant strains o f laboratory mice infected with Borrelia burgdorferi. Am. J. Trop. Med. Hyg. 47:249258.
5. Aron, L.,, M. Alekshun,, L. Perlee,, I. Schwartz,, H. Godfrey,, and F. Cabello. 1994. Cloning and DNA sequence analysis of bmpC, a gene encoding a potential membrane lipoprotein of Borrelia burgdorferi. FEMS Microbiol. Lett. 123:7582.
6. Asbrink, E.,, and A. Hovmark. 1985. Successful cultivation of spirochetes from skin lesions of patients with erythema chronica migrans afzelius and acrodermatitis chonica atrophicans. Acta Pathol. Microbiol. Immunol. Scand. 93:161163.
7. Backenson, P. B.,, J. L. Coleman ,, and J. L. Benach. 1995. Borrelia burgdorferi shows specificity of binding to glycosphingolipids. Infect. Immun. 63:28112817.
8. Balmelli, T.,, and J. C. Piffaretti. 1995. Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato. Res. Microbiol. 146:329340.
9. Barbour, A. G.,, S. L. Tessier,, and W. J. T odd. 1983. Lyme disease spirochetes and Ixodid tick spirochetes share a common surface antigenic determinant defined by monoclonal antibody. Infect. Immun. 41:795804.
10. Barthold, S. W. 1993. Antigenic stability of Borrelia burgdorferi during chronic infections of immunocompetent mice. Infect. Immun. 61:49554961.
11. Barthold, S. W. 1996. The globilisation of Lyme borreliosis. Lancet 348:16031604.
12. Barthold, S. W. 1991. Infectivity of Borrelia burgdorferi relative to route of inoculation and genotype in laboratory mice. J. Infect. Dis. 163: 419420.
13. Barthold, S. W. 1999. Specificity of infectioninduced immunity among Borrelia burgdorferi sensu lato species. Infect. Immun. 67:3642.
14. Barthold, S. W. 1998. Spirochetes, ticks, and DNA. Parasitol. Today 14:444.
15. Barthold, S. W.,, and L. K. Bockenstedt. 1993. Passive immunizing activity of sera from mice infected with Borrelia burgdorferi. Infect. Immun. 61:46964702.
16. Barthold, S. W.,, M. de Souza ,, and S. Feng. 1996. Serum-mediated resolution of Lyme arthritis in mice. Lab. Investig. 74:5767.
17. Barthold, S. W.,, M. S. deSouza,, J. L. Janotka,, A. L. Smith,, and D. H. Persing. 1993. Chronic Lyme borreliosis in the laboratory mouse. Am. J. Pathol. 143:951971.
18. Barthold, S. W.,, S. Feng ,, L. K. Bockenstedt,, E. Fikrig,, and K. Feen. 1997. Protective and arthritis-resolving activity in serum from mice infected with Borrelia burgdorferi. Clin. Infect. Dis. 25:S9S17.
19. Barthold, S. W.,, E. Fikrig,, L. K. Bockenstedt,, and D. H. Persing. 1995. Circumvention of outer surface protein A immunity by host-adapted Borrelia burgdorferi. Infect. Immun. 63: 22552261.
20. Barthold, S. W.,, S. A. Levy,, E. Fikrig,, L. K. Bockenstedt ,, and A. L. Smith. 1995. Serologic response of naturally exposed or vaccinated dogs to Borrelia burgdorferi, the agent o f Lyme borreliosis. J. Am. Vet. Med. Assoc. 207:14351440.
21. Barthold, S. W.,, D. H. Persing,, A. L. Armstrong,, and R. A. Peeples. 1991. Kinetics of Borrelia burgdorferi dissemination and evolution of disease following intradermal inoculation of mice. Am. J. Pathol. 139:263273.
22. Barthold, S. W.,, C. L. Sidman,, and A. L. Smith. 1992. Lyme borreliosis in genetically resistant and susceptible mice with severe combined immunodeficiency. Am. J. Trop. Med. Hyg. 47: 605613.
23. Bergstrom, S.,, C. F. Garon,, and A. G. Barbour. 1992. Extrachromosomal elements of spirochetes. Res. Microbiol. 143:623628.
24. Bledsoe, H. A.,, J. A. Carroll,, T. R. Whelchel,, M. A. Farmer,, D. W. Dorward,, and F. C. Gherardini. 1994. Isolation and partial characterization of Borrelia burgdorferi inner and outer membranes by using isopycnic centrifugation. J. Bacteriol. 176:74477455.
25. Bockenstedt, L. K.,, E. Hodzic ,, S. Feng,, K. W. Bourell,, A. deSilva,, R. Montgomery ,, J. D. Radolf,, and S. W. Barthold. 1997. Borrelia burgdorferi strain-specific OspC-mediated immunity in mice. Infect. Immun. 65:46614667.
26. Brorson, O.,, and S. H. Brorson. 1998. In vitro conversion of Borrelia burgdorferi to cystic forms in spinal fluid, and transformation to mobile spirochetes by incubation in BSK-H medium. Infection 26:144150.
27. Brorson, O.,, and S. H. Brorson. 1997. Transformation of cystic forms of Borrelia burgdorferi to normal, mobile spirochetes. Infection 25: 240246.
28. Brouqui, P.,, S. Badiaga,, and D. Raoult. 1996. Eukaryotic cells protect Borrelia burgdorferi from the action of penicillin and ceftriaxone but not from the action of doxycline and erythromycin. Antimicrob. Agents Chemother. 40:15521554.
29. Bruck, D. K.,, M. L. Talbor,, R. G. Cluss,, and J. T. Booth by. 1995. Ultrastructural characterization of the stages of spheroplast preparation of Borrelia burgdorferi. J. Microbiol. Methods 23:219.
30. Brunet, L. R.,, A. Spielman,, E. Fikrig,, and S. R. Telford III. 1997. Heterogeneity of Lyme disease spirochaetes within individual vector ticks. Res. Microbiol. 148:437445.
31. Burgdorfer, W.,, S. Hayes,, and J. Benach. 1988. Development of Borrelia burgdorferi in ixodid tick vectors. Ann. N. Y. Acad. Sci. 539:172179.
32. Burgdorfer, W.,, and S. F. Hayes. 1989. Vector- spirochete relationships in louse-borne and tick-borne borreliosis with emphasis on Lyme disease. Adv. Dis. Vector Res. 6:127150.
33. Burgdorfer, W.,, S. F. Hayes,, and D. Corwin. 1989. The pathophysiology of the Lyme disease spirochete, Borrelia burgdorferi, in ixodid ticks. Rev. Infect. Dis. 110:S1442S1450.
34. Burkot, T. R.,, J. Piesman,, and R. A. Wirtz,. 1994. Kinetics of the Borrelia burgdorferi outer surface protein A (OspA) in the tick, Ixodes scapularis, p. 224227. In R. Cevinini,, V. Sambri,, and M. LaPlaca (ed.), Advances in Lyme Borreliosis Research. Proceedings of the Vlth International Conference on Lyme Borreliosis. University of Bologna, Bologna, Italy.
35. Carter, C. J.,, S. Bergstrom,, S.J. Norris,, and A. G. Barbour. 1994. A family of surface-exposed proteins of 20 kilodaltons in the genus Borrelia. Infect. Immun. 62:27922799.
36. Casjens, S.,, G. Hatfull,, and R. Hendrix. 1992. Evolution of dsDNA tailed-bacteriophage genomes. Semin. Virol. 3:383397.
37. Casjens, S.,, R. van Vugt,, K. Tilly,, P. A. Rosa,, and B. Stevenson. 1997. Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes. J. Bacteriol. 179:217227.
38. Champion, C. I.,, D. R. Blanco,, J. T. Skare,, D. A. Haake,, M. Giladi,, D. Foley,, J. N. Miller,, and M. A. Lovett. 1994. A 9.0 kilobasepair circular plasmid of Borrelia burgdorferi encodes an exported protein: evidence for expression only during infection. Infect. Immun. 62:26532661.
39. Chang, Y. F.,, M. J. G. Appel,, R. H. Jacobson,, S. J. Shin,, P. Harpending,, R. Straubinger,, L. A. Patrician,, H. Mohammed ,, and B. A. Summers. 1995. Recombinant OspA protects dogs against infection and disease caused by Borrelia burgdorferi. Infect. Immun. 63:35433549.
40. Cheetham, B.,, and M. Katz. 1995. A role for bacteriophages in the evolution and transfer of bacterial virulence determinants. Mol. Microbiol. 18:201208.
41. Cinco, M.,, E. Banfi,, D. Balanzin,, C. Godeas,, and E. Panfili. 1991. Evidence for (Hpo) oligosaccharides in Borrelia burgdorferi and their surrounding serological specificity. FEMS Microbiol. Lett. 76:3338.
42. Coburn, J.,, L. Magoun,, S. C. Bodary,, and J. M. Leong. 1998. Integrins alpha(v)beta and alpha5betal mediate attachment of Lyme disease spirochetes to human cells. Infect. Immun. 66:19461952.
43. Coleman, J. L.,, and J. L. Benach. 1987. Isolation of antigenic components from the Lyme disease spirochete: their role in early diagnosis. J. Infect. Dis. 155:756765.
44. Coleman, J. L.,, J. A. Gebbia,, J. Piesman,, J. L. Degen,, T. H. Bugge,, and J. L. Benach. 1997. Plasminogen is required for efficient dissemination of Borrelia burgdorferi in ticks and for enhancement of spirochetemia in mice. Cell 89: 11111119.
45. Comstock, L. E.,, and D. D. Thomas. 1991. Characterization of Borrelia burgdorferi invasion of cultured endothelial cells. Microb. Pathog. 10: 137148.
46. Coughlin, R. T.,, D. Fish,, T. N. Mather,, J. N. Ma,, C. Pavia,, and P. Bulger. 1995. Protection of dogs from Lyme disease with a vaccine containing outer surface protein (Osp) A, OspB and the saponin adjuvant QS21. J. Infect. Dis. 171: 10491052.
47. Cox, C. L.,, D. R. Akins,, K. W. Bourell,, P. Lahdenne,, M. V. Norgard,, and J. D. Radolf. 1996. Limited surface exposure of Borrelia burgdorferi outer surface lipoproteins. Proc. Natl. Acad. Sci. USA 93:79737978.
48. Das, S.,, S. W. Barthold,, S. S. Giles,, R. R. Montgomery ,, S. R. Telford III,, and E. Fikrig. 1997. Temporal pattern of Borrelia burgdorferi p21 gene expression in ticks and the mammalian host. J. Clin. Investig. 99:987995.
49. Defosse, D. L.,, P. H. Duray,, and R. C. Johnson. 1992. The N I H-3 immunodeficient mouse is a model for Lyme borreliosis myositis and carditis. Am. J. Pathol. 141:310.
50. Demaerschalck, I.,, A. BenMessaoud,, M. DeKesel,, B. Hyois,, Y. Lobet,, P. Hoet,, G. Bigaignon,, A. Bollen,, and E. Godfroid. 1995. Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids o f Lyme disease patients. J. Clin. Microbiol. 33:602608.
51. deSilva, A.,, and E. Fikrig. 1995. Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding. Am. J. Trop. Med. Hyg. 53: 397404.
52. deSilva, A.,, E. Fikrig,, E. Hodzic,, S. R. Telford III,, and S. W. Barthold. 1998. Immune evasion by tick-borne and host-adapted Borrelia burgdorferi. J. Infect. Dis. 177:395400.
53. deSilva, A.,, S. R. Telford,, L. R. Brunet,, S. W. Barthold,, and E. Fikrig. 1996. Borrelia burgdorferi OspA arthropod-specific transmission-blocking Lyme disease vaccine. J. Exp. Med. 183: 271275.
54. deSilva, A. M.,, and E. Fikrig. 1997. Arthropod- and host-specific gene expression by Borrelia burgdorferi. J. Clin. Investig. 99:377379.
55. deSilva, A. M.,, D. Fish,, T. R. Burkot,, Y. Zhang,, and E. Fikrig. 1997. OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks. Infect. Immun. 65:31463150.
56. deSouza, M. S.,, E. Fikrig,, A. L. Smith,, R. A. Flavell,, and S. W. Barthold. 1992. Nonspecific proliferative responses of murine lymphocytes to Borrelia burgdorferi antigens. J. Infect. Dis. 165: 471478.
57. deSouza, M. S.,, A. L. Smith ,, D. S. Beck,, G. A. Terwilliger,, E. Fikrig,, and S. W. Barthold. 1993. Long-term study of cell-mediated responses to Borrelia burgdorferi in the laboratory mouse. Infect. Immun. 61:18141822.
58. Dorward, D. W. 1998. Immune ultrastructural evidence of lymphocytic antigen binding by Lyme disease spirochetes. Scanning 20:197199.
59. Dorward, D. W.,, E. R. Fischer,, and D. M. Brooks. 1997. Invasion and cytopathic killing of human lymphocytes by spirochetes causing Lyme disease. Clin. Infect. Dis. 25:S22S8.
60. Dorward, D. W.,, E. D. Huegenel,, G. Davis,, and C. F. Garon. 1992. Interactions between extracellular Borrelia burgdorferi proteins and non-Borrelia-directed immunoglobulin M antibodies. Infect. Immun. 60:838844.
61. Dressier, F.,, J. A. Whalen,, B. N. Reinhardt,, and A. C. Steere. 1993. Western blotting in the serodiagnosis of Lyme disease. J. Infect. Dis. 167: 392400.
62. Ebnet, K.,, K. D. Brown,, U. K. Siebenlist,, M. M. Simon,, and S. Shaw. 1997. Borrelia burgdorferi activates nuclear factor-KB and is a potent inducer of chemokine and adhesion molecule gene expression in endothelial cells and fibroblasts. J. Immunol. 158:32853292.
63. Erdile, L. F.,, M. A. Brandt,, D. J. Warakomski,, G. J. Westrack,, A. Sadziene,, A. G. Barbour,, and J. P. Mays. 1993. Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA. Infect. Immun. 61:8190.
64. Feng, S.,, E. Hodzic,, B. Stevenson,, and S. W. Barthold. 1998. Humoral Immunity to Borrelia burgdorferi N 4 0 decorin binding proteins during infection of laboratory mice. Infect. Immun. s66: 28272835.
65. Fikrig, E.,, S. W. Barthold,, F. S. Kantor,, and R. A. Flavell. 1990. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science 250:553556.
66. Fikrig, E.,, S. W. Barthold,, W. Sun,, W. Feng,, S.R.I. Telford ,, and R. A. Flavell. 1997. Borrelia burgdorferi P35 and P37 proteins, expressed in vivo, elicit protective immunity. Immunity 6: 531539.
67. Fikrig, E.,, L. K. Bockenstedt ,, S. W. Barthold ,, M. Chen,, H. Tao,, P. Ali-Salaam,, S. R. Telford,, and R. A. Flavell. 1994. Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis. J. Infect. Dis. 169:568574.
68. Fikrig, E.,, M. Chen,, S. W. Barthold,, J. Anguita,, W. Feng,, S. R. Telford III,, and R. A. Flavell. 1999. Borrelia burgdorferi erpT expression in the arthropod vector and murine host. Mol. Microbiol. 31:281290.
69. Fikrig, E.,, S. R. Telford III,, S. W. Barthold,, F. S. Kantor,, A. Spielman,, and R. A. Flavell. 1992. Elimination of Borrelia burgdorferi from vector ticks feeding on OspA-immunized mice. Proc. Natl. Acad. Sci. USA 89:54185421.
70. Fikrig, E.,, S. R. Telford in,, R. Wallich,, M. Chen,, Y. Lobet,, F. R. Matuschka,, R. B. Kimsey,, F. S. Kantor,, S. W. Barthold,, A. Spielman,, and R. A. Flavell. 1995. Vaccination against Lyme disease caused by diverse Borrelia burgdorferi. J. Exp. Med. 181:215221.
71. Fingerle, V.,, U. Hauser,, G. Liegl,, B. Petko,, V. Preac-Mursic,, and B. Wilske. 1995. Expression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus. J. Clin. Microbiol. 33:18671869.
72. Fraser, C. M.,, S. Casjens,, W. M. Huang ,, G. G. Sutton,, R. Clayton,, R. Lathigra,, O. White,, K. A. Ketchum,, R. Dodson ,, E. K. Hickey ,, M. Gwinn,, B. Dougherty,, J. F. Tomb,, R. D. Fleischmann,, D. Richardson,, J. Peterson,, A. R. Kerlavage,, J. Quackenbush,, S. Salzberg,, M. Hanson ,, R. van Vugt,, N. Palmer,, M. D. Adams,, J. Gocayne,, J. Weidman,, T. Utterback,, L. Watthey,, L. Mc Donald,, P. Artiach,, C. Bowman,, S. Garland,, C. Fujii,, M. D. Cotton,, K. Horst,, K. Roberts,, B. Hatch,, H. O. Smith,, and J. C. Venter. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390:580586.
73. Garon, C. F.,, D. W. Dorward,, and M. D. Corwin. 1989. Structural features of Borrelia burgdorferi—the Lyme disease spirochete: silver staining for nucleic acids. Scanning Microsc. 3:109115.
74. Gern, L.,, O. Rais,, C. Capiau,, P. Hauser,, Y. Lobet,, E. Simoen,, P. Voet,, and J. Petre. 1994. Immunization of mice by recombinant OspA preparations and protection against Borrelia burgdorferi infection induced by Ixodes ricinus ticks. Immunol. Lett. 39:249258.
75. Gern, L.,, U. E. Schaible,, and M. M. Simon. 1993. Mode of inoculation of the Lyme disease agent Borrelia burgdorferi influences infection and immune responses in inbred strains of mice. J. Infect. Dis. 167:971975.
76. Giambartolomei, G. H.,, V. A. Dennis,, B. L. Lasater,, and M. T. Philipp. 1999. Induction of pro- and anti-inflammatory cytokines by Borrelia burgdorferi lipoproteins in monocytes is mediated by CD14. Infect. Immun. 67:140147.
77. Gilmore, R. D., Jr.,, and M. L. Mbow. 1998. A monoclonal antibody generated by antigen inoculation via tick bite is reactive to Borrelia burgdorferi Rev protein, a member of the 2.9 gene family locus. Infect. Immun. 66:980986.
78. Girschick, H. J.,, H. I. Huppertz,, H. Russmann,, V. Krenn,, and H. Karch. 1996. Intracellular persistence of Borrelia burgdorferi in human synovial cells. Rheumatol. Int. 16:125132.
79. Golde, W. T.,, K. J. Kappel,, G. Dequesne,, C. Feron,, D. Plainchamp,, C. Capiau,, and Y. Lobet. 1994. Tick transmission of Borrelia burgdorferi to inbred strains o f mice induces an antibody response to P39 but not to outer surface protein A. Infect. Immun. 62:26252627.
80. Goldstein, S. F.,, N. W. Charon,, and J. A. Kreiling. 1994. Borrelia burgdorferi swims with a planar waveform similar to that of eukaryotic flagella. Proc. Natl. Acad. Sci. USA 91:34333437.
81. Goodman, J. L.,, P. Jurkovich,, C. Kodner,, and R. C. Johnson. 1991. Persistent cardiac and urinary tract infections with Borrelia burgdorferi in experimentally infected Syrian hamsters. J. Clin. Microbiol. 29:894896.
82. Grab, D. J.,, C. Givens,, and R. Kennedy. 1998. Fibronectin-binding activity in Borrelia burgdorferi. Biochim. Biophys. Acta 1407:135145.
83. Guttman, D. S.,, P. W. Wang,, I. N. Wang,, E. M. Bosler,, B. J. Luft,, and D. E. Dykhuizen. 1996. Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by singlestrand conformation polymorphism analysis. J. Clin. Microbiol. 34:652656.
84. Hanson, M. S.,, D. R. Cassatt,, B. P. Guo,, N. K. Patel,, M. P. McCarthy,, D. W. Dorward,, and M. Hook. 1998. Active and passive immunity against Borrelia burgdorferi decorin binding protein A (DbpA) protects against infection. Infect. Immun. 66:21432153.
85. Hofmeister, E. K.,, and J. E. Childs. 1995. Analysis of Borrelia burgdorferi sequentially isolated from Peromyscus leucopus captured at a Lyme disease enzootic site. J. Infect. Dis. 172:462469.
86. Hu, L. T.,, S. D. Pratt,, G. Perides,, L. Katz,, R. A. Rogers,, and M. S. Klempner. 1997. Isolation, cloning, and expression of a 70-kilodalton plasminogen binding protein of Borrelia burgdorferi. Infect. Immun. 65:49894995.
87. Hulinska, D.,, P. Bartak,, J. Hercogova ,, J. Hancil,, J. Basta,, and J. Schramlova. 1994. Electron microscopy of Langerhans cells and Borrelia burgdorferi in Lyme disease patients. Zentbl. Bakteriol. 280:348359.
88. Indest, K. J.,, R. Ramamoorthy,, M. Sole,, R. D. Gilmore ,, B. J. B. Johnson ,, and M. T. Philip p. 1997. Cell-density-dependent expression of Borrelia burgdorferi lipoproteins in vitro. Infect. Immun. 65:11651171.
89. Jauris-Heipke, S.,, G. Liegl,, V. Preac-Mursic,, D. Rossler,, E. Schwab,, E. Spitscjel,, G. Will,, and B. Wilske. 1995. Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to OspA genotype and evidence of lateral gene exchange of OspC. J. Clin. Microbiol. 33:18601866.
90. Johnson, B. J. B.,, S. L. Sviat,, C. M. Happ,, J. J. Dunn,, J. C. Frantz,, L. W. Mayer,, and J. Piesman. 1995. Incomplete protection of hamsters vaccinated with unlipidated OspA from Borrelia burgdorferi infection is associated with low levels o f antibody to an epitope defined by mAb La- 2. Vaccine 13:10861094.
91. Kalish, R. A.,, J. M. Leong,, and A. C. Steere. 1995. Early and late antibody responses to full length and truncated constructs of outer surface protein A of Borrelia burgdorferi in Lyme disease. Infect. Immun. 63:22282235.
92. Kaneda, K.,, T. Masuzawa,, M. M. Simon,, E. Isogai,, H. Isogai,, K. Yasugarni,, T. Suzuki,, Y. Suzuki,, and Y. Yanagihara. 1998. Infectivity and arthritis induction of Borrelia japonica on SCID mice and immune competent mice: possible role of galactosylceramide binding activity on initiation of infection. Microbiol. Immunol. 42:171175.
93. Kersten, A.,, C. Poitschek,, S. Rauch ,, and E. Aberer. 1995. Effects of penicillin, ceftriaxone, and doxycycline on morphology of Borrelia burgdorferi. Antimicrob. Agents Chemother. 39:11271133.
94. Kimsey, R. B.,, and A. Spielman. 1990. M o tility of Lyme disease spirochetes in fluid as viscous as the extracellular matrix. J. Infect. Dis. 162: 12051208.
95. Kitten, T.,, and A. Barbour. 1990. Juxtaposition of expressed variable antigen genes with a conserved telomere in the bacterium Borrelia hermsii. Proc. Natl. Acad. Sci. USA 87:60776081.
96. Klempner, M. S.,, R. Noring,, M. P. Epstein,, B. Mc Cloud,, and R. A. Rogers. 1996. Binding of human urokinase type plasminogen activator and plasminogen to Borrelia species. J. Infect. Dis. 174:97104.
97. Klempner, M. S.,, R. Noring,, and R. A. Rogers. 1993. Invasion of human skin fibroblasts by the Lyme disease spirochete, Borrelia burgdorferi. J. Infect. Dis. 167:10741081.
98. Kuiper, H.,, A. P. van Dam,, L. Spanjaard,, B. M. de Jongh,, A. Widjojokusumo,, T. C. P. Ramselaar,, I. Cairo,, K. Vos,, and J. Dankert. 1994. Isolation of Borrelia burgdorferi from biopsy specimens taken from healthy-looking skin of patients with Lyme borreliosis. J. Clin. Microbiol. 32:715720.
99. Lahdenne, P.,, S. F. Porcella,, K. E. Hagman,, D. R. Akins,, T. G. Popova,, D. D. Cox,, L. I. Katona,, J. D. Radolf,, and M. V. Norgard. 1997. Molecular characterization of 6.6-kilodalton Borrelia burgdorferi outer membrane-associated lipoprotein (lp6.6) which appears to be downregulated during mammalian infection. Infect. Immun. 65:412421.
100. Lam, T. T.,, T. K. Nguyen,, R. R. Montgomery,, F. S. Kantor,, E. Fikrig,, and R. A. Flavell. 1994. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infect. Immun. 62:290298.
101. LeFleche, A.,, D. Postic,, K. Girardet,, O. Peter,, and G. Baranton. 1997. Characterization of Borrelia lusitaniae sp. nov. by 16S ribosomal DNA sequence analysis. Int. J. Syst. Bacteriol. 47:921925.
102. Leong, J. M.,, H. Wang,, L. Magoun,, J. A. Field,, P. E. Morrissey,, D. Robbins,, J. B. Tatro J. Coburn, and N. Parveen. 1998. Different classes of proteoglycan contribute to the attachment of Borrelia burgdorferi to cultured endothelial and brain cells. Infect. Immun. 66: 994999.
103. Liveris, D.,, G. P. Wormser,, J. Nowakowski,, R. Nadelman,, S. Bittker,, D. Cooper,, S. Varde,, F. H. Moy,, G. Forsetter,, C. S. Pavia,, and I. Schwartz. 1996. Molecular typing of Borrelia burgdorferi from Lyme disease patients by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 34:13061309.
104. Livey, I.,, C. P. Gibbs,, R. Schuster,, and F. Dormer. 1995. The role of lateral transfer and recombination in OspC variation in Lyme disease Borrelia. Mol. Microbiol. 18:257269.
105. Lovrich, S. D.,, S. M. Callister,, B. K. Du-Chateau,, L. C. L. Lim,, J. Winfrey,, S. P. Day,, and R. F. Schell. 1995. Abilities of OspA proteins from different seroprotective groups of Borrelia burgdorferi to protect hamsters from infection. Infect. Immun. 63:21132119.
106. Ma, Y.,, K. P. Seiler,, K.-F. Tai,, L. Yang,, M. Woods,, and J. J. Weis. 1994. Outer surface lipoproteins of Borrelia burgdorferi stimulate nitric oxide production by the cytokine-inducible pathway. Infect. Immun. 62:36633671.
107. MacDonald, A. B. 1998. Concurrent neocortical borreliosis and Alzheimer's disease. Ann. N. Y. Acad. Sci. 539:468470.
108. Malawista, S. M.,, S. W. Barthold,, and D. H. Persing. 1994. Fate of Borrelia burgdorferi DNA in tissues of infected mice after antibiotic treatment. J. Infect. Dis. 170:13121316.
109. Marconi, R. T.,, D. S. Samuels,, R. K. Landry,, and C. F. Garon. 1994. Analysis of the distribution and molecular heterogeneity of the ospD gene among the Lyme disease spirochetes: evidence for lateral gene exchange. J. Bacteriol. 176:45724582.
110. Marti Ras, N.,, D. Postic,, M. Foretz,, and G. Baranton. 1997. Borrelia burgdorferi sensu stricto, a bacterial species "made in the U.S.A."? Int. J. Syst. Bacteriol. 47:11121117.
111. Mitchell, P. D.,, K. D. Reed,, and J. M. Hofkes. 1996. Immunoserologic evidence of coinfection with Borrelia burgdorferi, Babesia microti, and human granulocytic Ehrlichia species in residents of Wisconsin and Minnesota. J. Clin. Microbiol. 34:724727.
112. Modolell, M.,, U. E. Schaible,, M. Rittig,, and M. M. Simon. 1994. Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete. Immunol. Lett. 40:139146.
113. Montgomery, R. R.,, and S. E. Malawista. 1996. Entry of Borrelia burgdorferi into macrophages is end-on and leads to degradation in lysosomes. Infect. Immun. 64:28672872.
114. Montgomery, R. R.,, M. H. Nathanson,, and S. E. Malawista. 1993. The fate of Borrelia burgdorferi, the agent for Lyme disease, in mouse macrophages: destruction, survival, recovery. J. Immunol. 150:909915.
115. Moody, K. D.,, S. W. Barthold,, G. A. Terwilliger,, D. S. Beck,, G. M. Hansen,, and R. O. Jacoby. 1990. Experimental chronic Lyme borreliosis in Lewis rats. Am. J. Trop. Med. Hyg. 4 2: 6574.
116. Morrison, T. B.,, J. H. Weis,, and J. J. Weis. 1997. Borrelia burgdorferi outer surface protein A (OspA) activates and primes human neutrophils. J. Immunol. 158:48384845.
117. Nakao, M.,, and K. Miyamoto. 1995. Mixed infection of different Borrelia species among Apodemus speciosus mice in Hokkaido, Japan. J. Clin. Microbiol. 33:490492.
118. Nguyen, T. K.,, T. T. Lam,, S. W. Barthold,, S. R. Telford III,, R. A. Flavell,, and E. Fikrig. 1994. Partial destruction of Borrelia burgdorferi within ticks engorged on OspE- or OspF-immunized mice. Infect. Immun. 62:20792084.
119. Nocton, J. J.,, F. Dressier,, R. J. Rutledge,, P. N. Rys,, D. H. Persing,, and A. C. Steere. 1994. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis. N. Engl. J. Med. 330:229234.
120. Norgard, M.,, B. Riley,, J. Richardson,, and J. Radolf. 1995. Dermal inflammation elicited by synthetic analogs of Treponema pallidum and Borrelia burgdorferi lipoproteins. Infect. Immun. 63: 15071515.
121. Norgard, M. V.,, L. L. Arndt,, D. R. Akins,, L. L. Curetty,, D. A. Harrich,, and J. D. Radolf. 1996. Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides via a pathway distinct from that of lipopolysaccharide involves the transcriptional activator of N F-K B. Infect. Immun. 64:38453852.
122. Norris, S. J.,, C.J. Carter,, J. K. Howell,, and A. G. Barbour. 1992. Low-passage associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface exposed, plasmid-encoded lipoprotein. Infect. Immun. 60:46624672.
123. Oksi, J.,, M. Marjamaki,, K. Koski,, J. Ni-koskelainen,, and M. Viljanen. 1995. Bilateral facial palsy and meningitis caused by borrelia double infection. Lancet 345:15831584.
124. Pachner, A. R.,, J. Basta,, E. Delaney,, and D. Hulinska. 1995. Localization of Borrelia burgdorferi in murine Lyme borreliosis by electron microscopy. Am. J. Trop. Med. Hyg. 52:128133.
125. Pachner, A. R.,, E. Delaney,, and T. O Neill. 1995. Neuroborreliosis in the nonhuman primate: Borrelia burgdorferi persists in the central nervous system. Ann. Neurol. 38:667669.
126. Padula, S. J.,, F. Dias,, A. Sampieri,, R. B. Craven,, and R. W. Ryan. 1994. Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease. J. Clin. Microbiol. 32:17331738.
127. Persing, D. H.,, D. Mathiesen,, D. Podzorski,, and S. W. Barthold. 1994. Genetic stability of Borrelia burgdorferi recovered from chronically infected immunocompetent mice. Infect. Immun. 62:35213527.
128. Persing, D. H.,, B. J. Rutledge,, P. N. Rys,, D. S. Podzorski,, P. D. Mitchell,, K. D. Reed,, B. Liu,, E. Fikrig,, and S. E. Malawista. 1994. Target imbalance: disparity of Borrelia burgdorferi genetic material in synovial fluid from Lyme arthritis patients. J. Infect. Dis. 169:668672.
129. Philipp, M. T.,, M. K. Aydintug,, R. P. Bohm, Jr.,, F. B. Cogswell,, V. A. Dennis,, H. N. Lanners,, R. C. Lowrie, Jr.,, E. D. Roberts,, M. D. Conway,, M. Karacorlu,, G. A. Peyman,, D. J. Gubler,, B. J. B. Johnson,, J. Piesman,, and Y. Gu. 1993. Early and early disseminated phases o f Lyme disease in the rhesus monkey: a model for infection in humans. Infect. Immun. 61:30473059.
130. Philipp, M. T.,, Y. Lobet,, R. P. Bohm, Jr.,, E. D. Roberts,, V. A. Dennis,, Y. Gu,, R. C. Lowrie, Jr.,, P. Desmons,, P. H. Duray,, J. D. Englands,, P. Hauser,, J. Piesman,, and K. Xu. 1997. The outer surface protein A (OspA) vaccine against Lyme disease: efficacy in the rhesus monkey. Vaccine 15:18721887.
131. Phillips, S. E.,, L. H. Mattman,, D. Hulinska,, and H. Moayad. 1998. A proposal for the reliable culture of Borrelia burgdorferi from patients with chronic Lyme disease, even from those previously aggressively treated. Infection 26:364367.
132. Piesman, J.,, M. C. Dolan,, C. M. Happ,, B. J. Luft,, S. E. Rooney,, T. N. Mather,, and W. T. Golde. 1997. Duration of immunity to reinfection with tick-transmitted Borrelia burgdorferi in naturally infected mice. Infect. Immun. 65:40434047.
133. Porcella, S. F.,, T. G. Popova,, D. R. Akins,, M. Y. Li,, J. D. Radolf,, and M. V. Norgard. 1996. Borrelia burgdorferi supercoiled plasmids encode multicopy tandem open reading frames and a lipoprotein gene family. J. Bacteriol. 178:32933307.
134. Preac-Mursic, V.,, H. W. Pfister,, B. Wilske,, B. Gross,, A. Baumann,, and J. Prokop. 1989. Survival of Borrelia burgdorferi in antibiotically treated patients with Lyme borreliosis. Infection 17:355359.
135. Preac-Mursic, V.,, G. Wanner,, S. Reinhardt,, B. Wilske,, U. Busch,, and W. Marget. 1996. Formation and cultivation of Borrelia burgdorferi spheroplast-L-form variants. Infection 24: 218226.
136. Probert, W. S.,, M. Crawford,, R. B. Cadiz,, and R. B. LeFebvre. 1997. Immunization with outer surface protein (Osp) A, but not OspC, provides cross-protection of mice challenged with North American isolates of Borrelia burgdorferi. J Infect. Dis. 175:400405.
137. Radolf, J. D.,, L. L. Arndt,, D. R. Akins,, L. L. Curetty,, M. E. Levi,, Y. N. Shen,, L. S. Davis,, and M. V. Norgard. 1995. Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides activate monocytes/macrophages. J. Immunol. 154:28662877.
138. Ramamoorthy , R.,, and M. T. Philipp. 1998. Differential expression of Borrelia burgdorferi proteins during growth in vitro. Infect. Immun. 66: 51195124.
139. Ramamoorthy, R.,, L. Povinelli,, and M. T. Philipp. 1996. Molecular characterization, genomic arrangement, and expression of bmpD, a new member of the bmp class of genes encoding membrane proteins of Borrelia burgdorferi. Infect. Immun. 64:12591264.
140. Ribeiro, J. M. C.,, T. N. Mather,, J. Piesman,, and A. Spielman. 1987. Dissemination and salivary delivery of Lyme disease spirochetes in vector ticks (Acari: Ixodidae). J. Med. Entomol. 24: 201205.
141. Rijpkema, S. G. T.,, M. J. C. H. Molkenboer,, L. M. Schouls,, F. Jongejan,, and J. F. P. Schellekens. 1995. Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33:30913095.
142. Roberts, E. D.,, R. F. Bohm,, F. B. Cogswell,, H. Norbert-Lanners,, R. C. Lowrie,, L. Povinelli,, J. Piesman,, and M. T. Philipp. 1995. Chronic Lyme disease in the rhesus monkey. Lab. Investig. 72:146160.
143. Roberts, W. C.,, B. A. Mullikin,, R. Lathigra,, and M. S. Hanson. 1998. Molecular analysis of sequence heterogeneity among genes encoding decorin binding proteins A and B of Borrelia burgdorferi sensu lato. Infect. Immun. 66:52755285.
144. Roehrig, J. T.,, J. Piesman,, A. R. Hunt,, M. G. Keen,, C. M. Happ,, and B. J. B. Johnson. dorferi in murine Lyme borreliosis by electron microscopy. Am. J. Trop. Med. Hyg. 52:128133.
145. Rosa, P. A.,, T. Schwan,, and D. Hogan. 1992. Recombination between genes encoding major outer surface proteins A and B of Borrelia burgdorferi. Mol. Microbiol 6:30313040.
146. Sadziene, A.,, P. Rosa,, D. Hogan,, and A. Barbour. 1992. Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization. J. Exp. Med. 176:799809.
147. Schaible, U. E.,, M. D. Kramer,, K. Eichmann,, M. Modolell,, C. Museteanu,, and M. M. Simon. 1990. Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proc. Natl. Acad. Sci. USA 87:37683772.
148. Schaible, U. E.,, M. D. Kramer,, C. Museteanu,, G. Zimmer,, H. Mossmann,, and M. Simon. 1990. Lyme borreliosis in the severe combined immunodeficiency (scid) mouse manifests predominantly in the joints, heart and liver. Am. J. Pathol 137:811820.
149. Schaible, U. E.,, R. Wallich,, M. D. Kramer,, G. Nerz,, T. Stehle,, C. Museteanu,, and M. M. Simon. 1994. Protection against Borrelia burgdorferi infection in SCID mice is conferred by presensitized spleen cells and partially by B- but not T-cells alone. Intern. Immun. 6:671681.
150. Schaller, M.,, and U. Neubert. 1994. Ultrastructure of Borrelia burgdorferi after exposure to benzylpenicillin. Infection 22:401406.
151. Schmidli, J.,, T. Hunziker,, P. Moesli,, and U. B. Schaad. 1988. Cultivation of Borrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J. Infect. Dis. 158:905906.
152. Schwan, T. G. 1996. Ticks and Borrelia: Model systems for investigating pathogen-arthropod interactions. Infect. Agents Dis. 5:167181.
153. Schwan, T. G.,, W. Burgdorfer,, and C. F. Garon. 1988. Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect. Immun. 56:18311836.
154. Schwan, T. G.,, and B. J. Hinnebusch. 1998. Bloodstream-versus tick-associated variants of a relapsing fever bacterium. Science 280:19381940.
155. Schwan, T. G.,, J. Piesman,, W. T. Golde,, M. C. Dolan,, and P. A. Rosa. 1995. Induction of outer surface protein on Borrelia burgdorferi during tick-feeding. Proc. Natl. Acad. Sci. USA 92:29092913.
156. Seifert, H. S.,, and M. So. 1988. Genetic mechanisms of bacterial antigenic variation. Microbiol Rev. 52:327336.
157. Shih, C.-M.,, L.-P. Liu,, and A. Spielman. 1995. Differential spirochetal infectivities to vector ticks of mice chronically infected by the agent of Lyme disease. J. Clin. Microbiol. 33: 31643168.
158. Shoberg, R. J.,, and D. D. Thomas. 1995. Borrelia burgdorferi vesicle production occurs via a mechanism independent of immunoglobulin M. Infect. Immun. 63:48574861.
159. Simpson, W. J.,, W. Cieplak,, M. E. Schrumpf,, A. G. Barbour,, and T. G. Schwan. 1994. Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen. FEMS Microbiol Lett. 119:381388.
160. Simpson, W. J.,, C. R. Garon,, and T. G. Schwan. 1990. Borrelia burgdorferi contains repeated DNA sequences that are species specific and plasmid associated. Infect. Immun. 58:847853.
161. Steere, A. C. 1989. Lyme disease. N. Engl J. Med. 321:586596.
162. Stevenson, B.,, L. K. Bockenstedt,, and S. W. Barthold. 1994. Expression and gene sequence of outer surface protein C of Borrelia burgdorferi reisolated from chronically infected mice. Infect. Immun. 62:35683571.
163. Stevenson, B.,, J. L. Bono ,, A. Elias,, K. Tilly,, and P. Rosa. 1998. Transformation of the Lyme disease spirochete Borrelia burgdorferi with heterologous DNA. J. Bacteriol 180:48504855.
164. Stevenson, B.,, J. L. Bono,, T. G. Schwan,, and P. Rosa. 1998. Borrelia burgdorferi Erp proteins are immunogenic in mammals infected by tick bite, and their synthesis is inducible in cultured bacteria. Infect. Immun. 66:26482654.
165. Stevenson, B.,, S. Casjens,, and P. Rosa. 1998. Evidence of past recombination events among the genes encoding the Erp antigens of Borrelia burgdorferi. Microbiology 144:18691879.
166. Stevenson, B.,, T. G. Schwan,, and P. A. Rosa. 1995. Temperature-related differential expression of antigens in the Lyme disease spirochete, Borrelia burgdorferi. Infect. Immun. 63: 45354539.
167. Stevenson, B.,, K. Tilly,, and P. A. Rosa. 1996. A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31.J. Bacteriol 178:35083516.
168. Straubinger, R. K.,, B. A. Summers,, Y. F. Chang,, and M. J. G. Appel. 1997. Persistence of Borrelia burgdorferi in experimentally infected dogs after antibiotic treatment. J. Clin. Microbiol. 35:111116.
169. Suk, K.,, S. Das,, W. Sun,, B. Jwang,, S. W. Barthold,, R. A. Flavell,, and E. Fikrig. 1995. Borrelia burgdorferi genes selectively expressed in the infected host. Proc. Natl. Acad. Sci. USA 92:42694273.
170. Sung, S.-Y.,, C. L. Lavoie,, J. A. Carlyon,, and R. T. Marconi. 1998. Genetic divergence and evolutionary instability of ospE-related members of the upstream homology box gene family in Borrelia burgdorferi sensu lato complex isolates. Infect. Immun. 66:46564668.
171. Tai, K. F.,, Y. Ma,, and J. J. Weis. 1994. Normal human B lymphocytes and mononuclear cells respond to the mitogenic and cytokinestimulatory activities of Borrelia burgdorferi and its lipoprotein OspA. Infect. Immun. 62:520528.
172. Telford, S. R., III,, P. M. Armstrong,, P. Katavolos,, I. Foppa,, A. S. Olmeda-Garcia,, M. L. Wilson,, and A. Spielman. 1997. A new tick-borne encephalitis-like virus infecting New England deer ticks, Ixodes dammini. Emerg. Infect. Dis. 3:165170.
173. Theisen, M.,, B. Frederiksen,, A.-M. Lebech,, J. Vuust,, and K. Hansen. 1993. Polymorphism in the ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: implications for taxonomy and for use o f OspC protein as a diagnostic antigen. J. Clin. Microbiol. 31:25702576.
174. Theisen, M.,, M. Borre,, M. J. Mathiesen,, B. Mikkelsen,, A. M. Lebech,, and K. Hansen. 1995. Evolution of the Borrelia burgdorferi outer surface protein C. J. Bacteriol. 177:30363044.
175. van Dam, A. P.,, H. Kuiper,, K. Vos,, A. Widojojokusumo,, L. Spanjaard,, B. M. de Jongh,, A. C. P. Bamselaar,, M. D. Kramer,, and J. Dankert. 1993. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin. Infect. Dis. 17:708717.
176. Wallich, R.,, C. Brenner,, M. D. Kramer,, and M. M. Simon. 1995. Molecular cloning and immunological characterization of a novel linear-plasmid-encoded gene, pG, of Borrelia burgdorferi expressed only in vivo. Infect. Immun. 63: 33273335.
177. Wang, G.,, A. P. vandam,, A. LeFleche,, D . Postic,, O. Peter,, G. Baranton,, R. deBoer,, L. Spanjaard,, and J. Dankert. 1997. Genetic and phenotypic analysis of Borrelia valaisiana sp. nov. (Borrelia genomic groups VS116 and M l 9 J. Int. J. Syst. Bacteriol 47:926932.
178. Weis, J. J.,, Y. Ma,, and L. F. Erdile. 1994. Biological activities of native and recombinant Borrelia burgdorferi outer surface protein A: dependence on lipid modification. Infect. Immun. 62: 46324636.
179. Wheeler, C. M.,, J. C. Garcia-Monco,, M. G. Golightly,, G. S. Habicht,, and A. C. Steere. 1993. Nonprotein antigens of Borrelia burgdorferi. J. Infect. Dis. 167:665674.
180. Wilske, B.,, A. G. Barbour,, S. Bergstrom,, N. Burman,, B. I. Restrepo,, P. A. Rosa,, T. Schwan,, E. Soutschek,, and R. Wallich. 1992. Antigenic variation and strain heterogeneity in Borrelia spp. Res. Microbiol 143:583596.
181. Wilske, B.,, V. Preac-Mursic,, S. Jauris,, A. Hofman,, I. Pradel,, E. Soutschek,, E. Schwab,, E. Will,, and G. Wanner. 1993. Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi. Infect. Immun. 61: 21822191.
182. Wooten, R. M.,, V. R. Modur,, T. M. Mclntyre,, and J. J. Weis. 1996. Borrelia burgdorferi outer membrane protein A induces nuclear translocation of nuclear factor-KB and inflammatory activation in human endothelial cells. J. Bacteriol. 178:45844590.
183. Zhang, J.-R.,, J. M. Hardham,, A. G. Barbour,, and S.J. Norris. 1997. Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes. Cell 89:120.
184. Zhang, J. R.,, and S.J. Norris. 1998. Kinetics and in vivo induction of genetic variation of vlsE in Borrelia burgdorferi. Infect. Immun. 66: 36893697.
185. Zhong, W.,, T. Stehle,, C. Museteanu,, A. Siebers,, L. Gern,, M. Kramer,, R. Wallich,, and M. M. Simon. 1997. Therapeutic passive vaccination against chronic Lyme disease in mice. Proc. Natl Acad. Sci. USA 94: 1253312538.
186. Zung, J.,, S. Lewengrub,, M. Rudzinska,, A. Spielman,, S. Telford,, and J. Piesman. 1989. Fine structural evidence for the penetration of the Lyme disease spirochete Borrelia burgdorferi through the gut and salivary tissues o f Ixodes dammini. Can. J. Zool 67:17171748.

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