1887

Chapter 31 : A Love Affair with Streptomyces Genetics

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $7.00

Preview this chapter:
Zoom in
Zoomout

A Love Affair with Streptomyces Genetics, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818128/9781555811907_Chap31-1.gif /docserver/preview/fulltext/10.1128/9781555818128/9781555811907_Chap31-2.gif

Abstract:

One of the Ph.D. topics on offer for the author at the Cambridge University was the actinomycete . In the pneumococcus, and , incomplete genomes were transferred from donor to recipient strains by one of three bizarre processes (transformation, conjugation, and transduction) to yield incomplete zygotes, whereas fungi and higher organisms had life cycles, including a complete diploid stage and meiosis. Lewis Frost gave the author some cultures. The author streaked them out and chose one that produced a striking blue pigment. He set about isolating auxotrophic mutants from this strain in order to look for genetic recombination in the way it was done for . By the early 1950s, few microbiologists regarded the actinomycetes as fungi, but many still thought of them as intermediate between fungi and bacteria. For there were biochemical pointers to a bacterial affinity, but their cellular architecture was unclear. In the 1970s, the author's research group added artificial protoplast fusion to natural, plasmid-mediated conjugation as a means of doing genetics and then, much more important, the ability to transform protoplasts with plasmid or bacteriophage DNA, thus ushering in the in vitro years of gene cloning, which really put genetics on the map. Soon, numerous antibiotic resistance genes had been isolated, and basic aspects of gene expression worked out. They could manipulate the production of antibiotics by streptomycetes, which had enormous potential importance for the emergence of biotechnology in the pharmaceutical industry.

Citation: Hopwood D. 2000. A Love Affair with Streptomyces Genetics, p 243-250. In Atlas R (ed), Many Faces, Many Microbes. ASM Press, Washington, DC. doi: 10.1128/9781555818128.ch31

Key Concept Ranking

Bacterial Genetics
0.5108802
Streptomyces coelicolor
0.45116094
Pulsed-Field Gel Electrophoresis
0.42157152
0.5108802
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Figure 1
Figure 1

Teaching a UNIDO-sponsored course on Streptomyces genetics in Wuhan, China, April 1989.

Citation: Hopwood D. 2000. A Love Affair with Streptomyces Genetics, p 243-250. In Atlas R (ed), Many Faces, Many Microbes. ASM Press, Washington, DC. doi: 10.1128/9781555818128.ch31
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 2
Figure 2

Citation: Hopwood D. 2000. A Love Affair with Streptomyces Genetics, p 243-250. In Atlas R (ed), Many Faces, Many Microbes. ASM Press, Washington, DC. doi: 10.1128/9781555818128.ch31
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555818128.chap31
1. Redenbach, M.,, H. M. Kieser,, D. Denapaite,, A. Eichner,, J. Cullum,, H. Kinashi,, and D. A. Hopwood. 1996. A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol. Microbiol. 21:7796.
2. McDaniel, R.,, S. Ebert-Khosla,, D. A. Hopwood,, and C. Khosla. 1995. Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits. Nature 375:549554.
3. Lin, Y. S.,, H. M. Kieser,, D. A. Hopwood,, and C. W. Chen. 1993. The chromosomal DNA of Streptomyces lividans 66 is linear. Mol. Microbiol. 10:923933.
4. Hopwood, D. A.,, F. Malpartida,, H. M. Kieser,, H. Ikeda,, J. Duncan,, I. Fujii,, B. A. Rudd,, H. G. Floss,, and S. Omura. 1985. Production of "hybrid" antibiotics by genetic engineering. Nature 314:642644.
5. Malpartida, F.,, and D. A. Hopwood. 1984. Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature 309:462464.
6. Hopwood, D. A.,, H. M. Wright,, M. J. Bibb,, and S. N. Cohen. 1977. Genetic recombination through protoplast fusion in Streptomyces. Nature 268:171174.
7. Hopwood, D. A. 1959. Linkage and the mechanism of recombination in Streptomyces coelicolor. N.Y. Acad. Sci. 81:887898.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error