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Chapter 33 : It Came from Chicken Water
Cathy Squires worked on the regulation of ribosomal RNA (rRNA) synthesis and felt very fortunate to have had excellent students, postdoctoral fellows, and associates to share this project. They applied genetics, biochemistry, molecular biology, and physiology-the patchwork of his training and interests-to their studies of ribosome synthesis. Transcription regulation was studied using gene fusions to characterize a special feature of rRNA synthesis-transcription antitermination. Cell physiology and genetics were also studied as they relate to copies of ribosomal genes. A strain of Escherichia coli with no intact chromosomal rRNA operons was successfully constructed. The cell's only source of ribosomal genes is a plasmid. By exchanging coli sequences on the plasmid with those of other microbes, the author and his team were able to manipulate a cell's ribosome content in many unexpected ways and thereby pose novel questions about the cell's translation machinery.