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Chapter 12 : Diagnosis and Laboratory Techniques

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Abstract:

The India ink examination is a particularly useful and rapid diagnostic test for cryptococcal meningitis. Although can become fragile, collapse, or be crescentic when dried or fixed, the encapsulated cells are rapidly distinguished in a colored colloidal medium such as India ink when mixed with fluids such as cerebrospinal fluid (CSF). There are two clinical situations in which cultures of the CSF for identification of viable may be difficult. First, in some cases of chronic cryptococcal meningitis, the lumbar fluid is found to be culture negative. Second, some patients who receive an antifungal treatment course will have positive India ink examinations at the end of therapy despite clinical improvement. Serological tests for the diagnosis of cryptococcosis are one of the true success stories for rapid and accurate diagnosis of fungal infections in the last 30 to 40 years. The popularity of the diagnostic test for cryptococcal polysaccharide antigen is readily apparent when it is noted that there are at least five commercial latex agglutination kits for cryptococcal polysaccharide. DNA and RNA isolation, karyotyping, and DNA transformation techniques are discussed because they are more -specific; most other molecular techniques for working with are standard molecular biology procedures.

Citation: Casadevall A, Perfect J. 1998. Diagnosis and Laboratory Techniques, p 381-405. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch12

Key Concept Ranking

Latex Agglutination Test
0.5521935
Cryptococcal Antigen Test
0.45558837
Sodium Dodecyl Sulfate
0.4514696
Viruses
0.44568157
Pulsed-Field Gel Electrophoresis
0.41631284
0.5521935
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References

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1. Alspaugh, J. A.,, J. R. Perfect,, and J. Heitman. 1997. Cryptococcus neoformans mating and virulence are regulated by the G-protein gamma subunit GPA1 and cAMP. Genes Dev. 11:32063217.
2. Baughman, R. P.,, J. C. Rhodes,, M. N. Dohn,, H. Henderson,, and P. T. Frame. 1992. Detection of cryptococcal antigen in bronchoalveolar lavage fluid: a prospective study of diagnostic utility. Am. Rev. Respir. Dis. 145:12261229.
3. Bava, A. J.,, R. Negroni,, and M. Bianchi. 1993. Cryptococcosis produced by a urease negative strain of Cryptococcus neoformans. J. Med. Vet. Mycol. 31:8789.
4. Belay, T.,, R. Chemiak,, E. B. O'Neill,, and T. R. Kozel. 1996. Serotyping of Cryptococcus neoformans by dot enzyme assay. J. Clin. Microbiol. 34:466470.
5. Benham, R. W. 1950. Cryptococcosis and blastomycosis. Ann. N.Y. Acad. Sci. 50:12991314.
6. Bennett, J. E.,, H. F. Hasenclever,, and B. S. Tynes. 1964. Detection of cryptococcal polysaccharide in serum and spinal fluid: value in diagnosis and prognosis. Trans. Assoc. Am. Physicians 77:145150.
7. Berlin, L.,, and J. H. Pincus. 1989. Cryptococcal meningitis: false negative antigen test results and cultures in noninununosuppressed patients. Arch. Neurol. 46:13121316.
8. Bindschadler, D. D.,, and J. E. Bennett. 1968. Serology of human cryptococcosis. Ann. Intern. Med. 69:4552.
9. Blevins, L. B.,, J. Fenn,, H. Segal,, P. Newcomb-Gayman,, and K. C. Carroll. 1995. False-positive cryptococcal antigen latex agglutination caused by disinfectants and soaps. J. Clin. Microbiol 33:16741675.
10. Bloomfield, N.,, M. A. Gordon,, and D. F. Elmendorf, Jr. 1963. Detection of Cryptococcus neoformans antigen in body fluids by latex particle agglutination. Proc. Soc. Exp. Biol. Med. 114:6467.
11. Boom, W. H.,, D. J. Piper,, K. L. Ruoff,, and M. J. Ferraro. 1985. New cause for false-positive results with cryptococcal antigen test by latex agglutination. J. Clin. Microbiol. 22:856857.
12. Bottone, E. J.,, B. E. Johnasson,, M. Toma,, and G. P. Wormser. 1986. Poorly encapsulated Cryptococcus neoformans from patients with AIDS. I. Preliminary observations. AIDS Res. Hum. Retroviruses 2:221.
13. Brannon, P.,, and T. E. Kiehn. 1985. Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture. J. Clin. Microbiol. 22:951954.
14. Bruatto, M.,, V. Vidotto,, and A. M. Maine. 1992. Growth of Cryptococcus neoformans in tMamine-free medium. Mycopathologia 119:129132.
15. Buesching, W. J.,, K. Kurek,, and G. D. Roberts. 1979. Evaluation of the modified API 20C system for identification of clinically important yeasts. J. Clin. Microbiol 9:565569.
16. Campbell, C. K.,, A. L. Payne,, A. J. Teall,, A. Brownell,, and D. W. R. Mackenzie. 1985. Cryptococcal latex antigen test positive in patient with Trichosporon beigelii infection. Lancet ii:4344.
17. Campbell, G. D. 1966. Primary pulmonary cryptococcosis. Am. Rev. Respir. Dis. 94:236243.
18. Canteros, C. E.,, L. Rodero,, M. C. Rivas,, and G. Davel. 1996. A rapid urease test for presumptive identification of Cryptococcus neoformans. Mycopathologia 136:2123.
19. Chanock, S. J.,, P. Toltzis,, and C. Wilson. 1993. Cross-reactivity between Stomato-coccus mucilaginosus and latex agglutination for cryptococcal antigen. Lancet 342:11191120.
20. Chapin-Robertson, K.,, C. Bechtel,, S. Waycott,, C. Kdontnick,, and S. C. Edbeig. 1993. Cryptococcal antigen detection from urine of AIDS patients. Diagn. Microbiol Infect. Dis. 17:197201.
21. Chuck, S. L.,, and M. A. Sande. 1989. Infections with Cryptococcus neoformans in the acquired immunodeficiency syndrome. N. Engl. J. Med. 321:794799.
22. Clark, R. A.,, D. Greer,, W. Atkinson,, G. T. Valainis,, and N. Hyslop. 1990. Spectrum of Cryptococcus neoformans infection in 68 patients infected with acquired immunodeficiency virus. Rev. Infect. Dis. 12:768777.
23. Currie, B. P.,, L. F. Freundlich,, M. A. Soto,, and A. Casadevall. 1993. False-negative cerebrospinal fluid cryptococcal latex agglutination tests for patients with culture-positive cryptococcal meningitis, J. Clin. Microbiol. 31:25192522.
24. Davies, S. F.,, B. J. Gormus,, and R. Yarchoan. 1978. Cryptococcal meningitis with false-positive cytology in the CSF. JAMA 239:2369.
25. Denning, D. W.,, D. A. Stevens,, and J. R. Hamilton. 1990. Comparison of Guizotia abyssinica seed extract (birdseed) agar with conventional media for selective identification of Cryptococcus neoformans in patients with acquired immunodeficiency syndrome. J. Clin. Microbiol. 28:25652567.
26. Desmet, P.,, K. D. Kayembe,, and C. DeViroey. 1989. The value of cryptococcal serum antigen screening among HIV positive AIDS patients in Kinshasa, Zaire. AIDS 3:7778.
27. Diamond, R.D.,, and J. E. Bennett1974. Prognostic factors in cryptococcal meningitis. A studyof 111 cases. Ann. Intern. Med. 80:176181.
28. Dolan, C. T. 1972. Specificity of the latex cryptococcal antigen test. Am. J. Clin. Pathol. 58:358.
29. Drouhet, E.,, and M. Couteau. 1951. Sur les variations sectorielles des colonies de Torulopsis neoformans. Ann Inst. Pasteur 80:456457.
30. Dufait, R.,, R. Velho,, and C. de Vroey. 1987. Rapid identification of the two varieties of Cryptococcus neoformans by D-proline assimilation. Mykosen 30:483.
31. Dunn, W. M., Jr.,, G. Bhandarkar,, and D. Nafziger. 1995. Isolation of a nutritionally aberrant strain of Cryptococcus neoformans from a patient with AIDS. Clin. Infect. Dis. 21:15121513.
32. Dykstra, M. A.,, L. Friedman,, and J. W. Murphy. 1977. Capsule size of Cryptococcus neoformans: control and relationship to virulence. Infect. Immun. 16:129135.
33. Eng, R. H. K.,, E. Bishburg,, S. M. Smith,, and R. Kapila. 1986. Cryptococcal infections in patients with acquired immune deficiency syndrome. Am. J. Med. 81:1923.
34. Engler, H. D.,, and Y. R. Shen. 1994. Effect of potential interference factors on performance of enzyme immunoassay and latex agglutination assay for cryptococcal antigen. J. Clin. Microbiol. 32:23072308.
35. Erke, K. H.,, and J. D. Schneidau. 1973. Relationship of some Cryptococcus neoformans hypha-forming strains to standard strains and to other species of yeasts as determined by deoxyribonucleic acid base ratios and homologies. Infect. Immun. 7:941948.
36. Farmer, S. G.,, and R. A. Komorowski. 1973. Histologic response to capsule-deficient Cryptococcus neoformans. Arch. Pathol. 96:383387.
37. Feldmesser, M.,, C. Hams,, S. Reichberg,, S. Khan,, and A. Casadevall. 1996. Serum cryptococcal antigen in patients with AIDS. Clin. Infect. Dis. 23:827830.
38. Frank, U. K.,, S. L. Nishimura,, N. C. Li,, K. Sugai,, D. M. Yajko,, W. K. Hadley,, and V. L. Ng. 1993. Evaluation of an enzyme immunoassay for detection of cryptococcal capsular polysaccharide antigen in serum and cerebrospinal fluid. J. Clin. Microbiol. 31:97101.
39. Gade, W.,, S. W. Hinnefeld,, L. S. Babcock,, P. Gilligan,, W. Kelly,, K. Wait,, D. L. Greer,, M. Pinilla,, and R. L. Kaplan. 1991. Comparison of the premier cryptococcal antigen enzyme immunoassay and the latex agglutination assay for detection of cryptococcal antigens. J. Clin. Microbiol. 29:16161619.
41. Goldman, D.,, S. C. Lee,, and A. Casadevall. 1994. Pathogenesis of pulmonary Cryptococcus neoformans infection in the rat. Infect. Immun. 62:47554761.
42. Goldman, D. L.,, S. P. Franzot,, B. Fries,, and A. Casadevall. 1997. Phenotypic switching in the human pathogenic fungus Cryptococcus neoformans, abstr. F-44. 97th Annu. Meet. Am. Soc. Microbiol. 1997. American Society for Microbiology, Washington, D.C.
43. Gonyea, E. F. 1973. Cisternal puncture and cryptococcal meningitis. Arch. Neurol. 28:200201.
44. Goodman, J. S.,, L. Kaufman,, and M. G. Loening. 1971. Diagnosis of cryptococcal meningitis: detection of cryptococcal antigen. N. Engl. J. Med. 285:434436.
45. Gordon, M. A.,, and E. Lapa. 1971. Charcoal particle agglutination test for detection of antibody to Cryptococcus neoformans: a preliminary report. Am. J. Clin. Pathol. 56:354359.
46. Gordon, M. A.,, and E. W. Lapa. 1974. Elimination of rheumatoid factor in the latex test for cryptococcosis. Am. J. Clin. Pathol. 60:488494.
47. Gordon, M. A.,, and D. K. Vedder. 1966. Serologic tests in the diagnosis and prognosis of cryptococcosis. JAMA 197:961967.
48. Granger, D. L.,, J. R. Perfect,, and D. T. Durack. 1985. Virulence of Cryptococcus neoformans: regulation of capsule synthesis by carbon dioxide. J. Clin. Invest. 76:508516.
49. Gray, L. D.,, and G. D. Roberts. 1988. Experience with the use of pronase to eliminate interference factors in the latex agglutination test for cryptococcal antigen. J. Clin. Microbiol. 26:24502451.
50. Gutierrez, E.,, Y. S. Fu,, and H. Lurie. 1975. Cryptococcosis histologically resembling histoplasmosis: a light and electron microscopic study. Arch. Pathol. Lab. Med. 99:347.
51. Hamilton, J. R.,, A. Noble,, D. W. Denning,, and D. A. Stevens. 1991. Performance of cryptococcus antigen latex agglutination kits on serum and cerobrospinal fluid specimens of AIDS patients before and after pronase treatment. Clin. Microbiol. Rev. 29:333339.
52. Harding, S. A.,, W. M. Scheld,, P. S. Feldman,, and M. A. Sande. 1979. Pulmonary infection with capsule-deficient Cryptococcus neoformans. Virchows Arch. (A) 182:113118.
53. Hay, R. J.,, and D. W. R. Mackenzie. 1982. False-positive latex tests for cryptococcal antigen in cerebrospinal fluid. J. Clin. Pathol 35:244245.
54. Heelan, J. S.,, L. Corpus,, and N. Kessimian. 1991. False-positive reactions in the latex agglutination test for Cryptococcus neoformans antigen, J. Clin. Microbiol 29:12601261.
55. Hoffmann, S.,, J. Stenderup,, and L. R. Mathiesen. 1991. Low yield of screening for cryptococcal antigen by latex agglutination assay on serum and cerebrospinal fluid from Danish patients with AIDS on ARC. Scand. J. Infect. Dis. 23:697702.
56. Hopfer, R. L.,, P. Walden,, S. Setterquist,, and W. E. Highsmith. 1993. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis. J. Med. Vet. Mycol. 31:6575.
57. Hsu, C. Y. 1993. Cytologic diagnosis of pulmonary cryptococcosis in immunocompetent hosts. Acta Cytol. 37:667672.
58. Huahua, T.,, J. Rudy,, and C. M. Kunin. 1991. Effect of hydrogen peroxide on growth of Candida, Cryptococcus, and other yeasts in simulated blood culture bottles, J. Clin. Microbiol. 29:328332.
59. Huffnagle, K. E.,, and R. M. Gander. 1993. Evaluation of Gen-Probe's Histoplasma capsulatum and Cryptococcus neoformans AccuProbes. J. Clin. Microbiol. 31:419421.
60. Ikeda, R.,, R. Shinoda,, Y. Fukazawa,, and L. Kaufman. 1982. Antigenic characterization of Cryptococcus neoformans serotypes and its application of serotyping of clinical isolates, J. Clin. Microbiol. 16:2229.
62. Jacobson, E. S.,, and M. J. Petro. 1987. Extracellular iron chelation in Cryptococcus neoformans. J. Med. Vet. Mycol. 25:415418.
63. Jacobson, E. S.,, and S. E. Vartivarian. 1992. Iron assimilation in Cryptococcus neoformans. J. Med. Vet. Mycol. 30:443450.
64. Kabasawa, K.,, H. Itagaki,, R. Ikeda,, T. Shinoda,, K. Kagaya,, and Y. Fukazawa. 1991. Evaluation of a new method for identification of Cryptococcus neoformans which uses serologic tests aided by selected biological tests, J. Clin. Microbiol. 29:28732876.
65. Kanjanavirojkul, N.,, C. Sripa,, and A. Puapairoj. 1997. Cytologic diagnosis of Cryptococcus neoformans in HIV-positive patients. Acta Cytol. 41:493496.
66. Kaplan, W.,, S. L. Bragg,, S. Crane,, and D. G. Ahearn. 1981. Serotyping Cryptococcus neoformans by immunofluorescence. J. Clin. Microbiol. 14:313317.
67. Kauffman, L.,, and S. Blumen 1968. Value and interpretation of serologicaltests for the diagnosis of cryptococcosis. Appl. Microbiol. 16:19071912.
68. Kornfeld, S. L.,, and M. Worthington. 1980. False-positive CSF cryptococcal antigen tests. Arch. Neurol. 37:603605.
69. Kovacs, J. A.,, A. A. Kovacs,, M. Polis,, W. C. Wright,, V. J. Gill,, C. U. Tuazon,, E. P. Gelmann,, H. C. Lane,, R. Longfield,, G. Overturf,, A. M. Mâcher,, A. S. Fauci,, J. E. Parrillo,, J. E. Bennett,, and H. Masur. 1985. Cryptococcosis in the acquired immunodeficiency syndrome. Ann. Intern. Med. 103:533538.
70. Kwon-Chung, K.J.,, W. B. Hill,, and J. E. Bennett 1981. New special stain forhis-topathological diagnosis of cryptococcosis, J. Clin. Microbiol. 13:383387.
71. Kwon-Chung, K.J.,, I. Polacheck,, and J. E. Bennett 1982. Improved diagnostic medium forseparation of Cryptococcus neoformans var. neoformans (serotype Aand D) and Cryptococcus neoformans var. gattii (serotype B andC). J. Clin. Microbiol. 15:535537.
72. Lacaz, C. S.,, E. M. Heins-Vaccari,, N. T. Melo,, O. A. Moreno-Carvalho,, M. L. Sam-paio,, L. S. Nogueira,, R. J. Badaro,, and J. A. Livramento. 1993. Neurocryptococcosis caused by nonencapsulated Cryptococcus neoformans. Arq. Neuropsiquiatr. 51:395398.
73. Levinson, D. J.,, D. C. Silcox,, J. W. Rippon,, and S. Thomsen. 1974. Septic arthritis due to nonencapsulated Cryptococcus neoformans with co-existing sarcoidosis. Arthritis Rheum. 17:10371047.
74. Li, A.,, N. Guo,, and S. Wu. 1993. A strain of urease negative Cryptococcus neoformans isolated from the environment in China. Clin. Med. Set. J. 8:5254.
75. Liaw, Y.-S.,, P. -C Yang,, C.-J. Yu,, D.-B. Chang,, H.-J. Wang,, L.-N. Lee,, S.-H. Kuo,, and K.-T. Luh. 1995. Direct determination of cryptococcal antigen in transthoracic needle aspirate for diagnosis of pulmonary cryptococcosis. J. Clin. Microbiol. 33:15881591.
76. Littman, M. L. 1958. Capsule synthesis by Cryptococcus neoformans. Trans. N.Y. Acad. Sci. 20:623648.
77. Littman, M. L. 1959. Cryptococcosis (torulosis). Am. J. Med. 27:976998.
78. Louria, D. B.,, T. Kaminski,, M. Grieco,, and J. Singer. 1969. Aberrant forms of bacteria and fungi found in blood or cerebrospinal fluid. Arch. Intern. Med. 124:3948.
79. MacKinnon, S.,, J. G. Kane,, and R. H. Parken 1978. False positive cryptococcal antigentest and cervical prevertebral abscess. JAMA 240:19821983.
80. Maesaki, S.,, S. Kohno,, H. Mashinoto,, J. Araki,, S. Asai, and K. Hara. 1995. Detection of Cryptococcus neoformans in bronchial lavage cytology: report of four cases. Intern. Med. 34:5457.
80a. Malik, R.,, R. McPetrie,, D. I. Wigney,, A. J. Craig,, and D. N. Love. 1996. A latex cryptococcal antigen agglutination test for diagnosis and monitoring of therapy for cryptococcosis. Aust. Vet. J. 74:358364.
81. Manfredi, R.,, A. Moroni,, A. Mazzoni,, A. Nanetti,, M. Donati,, A. Mastroiannis,, O. V. Coronado,, and F. Chiodo. 1996. Isolated detection of Cryptococcal polysaccloride antigen in cerebrospinal fluid samples from patients with AIDS. Clin. Infect. Dis. 23:849850.
82. McManus, E. J.,, M. J. Bozdeck,, and J. M. Jones. 1985. Role of the latex agglutination test for cryptococcal antigen in diagnosing disseminated infections with Trichosporon beigelii. J. Infect. Dis. 151:11671169.
83. McManus, E. J.,, and J. M. Jones. 1985. Detection of a Trichosporon beigelii antigen cross-reactive with Cryptococcus neoformans capsular polysaccharide in serum from a patient with disseminated Trichosporon infection. J. Clin. Microbiol. 21:681685.
84. Mess, T.,, and E. S. Daar. 1997. Utility of fungal blood cultures for patients with AIDS. Clin. Infect. Dis. 25:13501353.
85. Milchgrub, S.,, E. Visconti,, and J. Avellini. 1990. Granulomatous prostatitis induced by capsule-deficient cryptococcal infection, J. Urol. 143:365366.
86. Millón, L.,, T. Barale,, M. C. Julliot,, J. Martinez,, and G. Mantón. 1995. Interference byhydroxyl starch used for vascular filling in latex agglutination test for cryptococcal antigen, J. Clin. Microbiol. 33:19171919.
87. Mitchell, T. G.,, E. Z. Freedman,, T. J. White,, and J. W. Taylor. 1994. Unique oligonucleotide primers in PCR for identification of Cryptococcus neoformans.J. Clin. Microbiol. 32:253255.
88. Moore, T.D.,, and J. C. Edman. 1993. The alpha-mating typelocus of Cryptococcus neoformans contains a peptide pheromone gene. Mol. Cell. Biol. 13:19621970.
89. Negroni, R.,, C. Cendoya,, A. I. Arechravala,, A. M. Robles,, M. Bianchi,, A. J. Bava,, and S. Helou. 1995. Detection of Cryptococcus neoformans capsular polysaccharide antigen in asymptomatic HIV-infected patients. Rev. Inst. Med. Trop. Sao Paulo 37:385389.
90. Nelson, M. R.,, M. Bower,, D. Smith,, C. Reed,, D. Shanson,, and B. Gazzard. 1990. The value of serum cryptococcal antigen in the diagnosis of cryptococcal infection in patients infected with the human immunodeficiency virus, J. Infect. Dis. 21:175181.
91. Nishikawa, M. M.,, O. D. SanfAnna,, M. S. Lazera,, and B. Wanke. 1996. Use of D-proline assimilation and CGB medium for screening Brazilian Cryptococcus neoformans isolates, J. Med. Vet. Mycol. 34:365366.
92. Odds, F. C.,, T. DeBacker,, G. Dams,, X. Yranck,, and F. Woestenboroghs. 1995. Oxygen as a limiting nutrient for growth of Cryptococcal neoformans. J. Clin. Microbiol. 33:995997.
93. Perfect, J. R.,, D. T. Durack,, and H. A. Gallis. 1983. Cryptococcemia. Medicine 62:98109.
94. Polacheck, I.,, and K. J. Kwon-Chung. 1986. Canavanine resistance in Cryptococcus neoformans. Antimicrob. Agents Chemother. 29:468473.
95. Powderly, W. G.,, G. A. Cloud,, W. E. Dismukes,, and M. S. Saag. 1994. Measurement of cryptococcal antigen in serum and cerebrospinal fluid: value in the management of AIDS-associated cryptococcal meningitis. Clin. Infect. Dis. 18:789792.
96. Prevost-Smith, E.,, and N. Hutton. 1994. Improved detection of Cryptococcus neoformans in the BACTEC NR 660 blood culture system. Microbiol. Infect. Dis. 102:741745.
97. Ro, J. Y.,, S. S. Lee,, and A. G. Ayala. 1987. Advantage of Fontana-Masson stain in capsule-deficient cryptococcal infection. Arch. Pathol. Lab. Med. 111:5357.
98. Roberts, G. D.,, and J. A. Washington II. 1975. Detection of fungi in blood cultures. J. Clin. Microbiol. 1:309310.
99. Robinson, R. G.,, M. J. Sulita,, E. K. Matthews,, and J. R. Warren. 1987. Failure of the BACTEC 460 radiometer to detect Cryptococcus neoformans in an AIDS patient. Am. J. Clin. Pathol. 87:783786.
100. Sekhon, A. S.,, A. K. Garg,, L. Kaufman,, G. S. Kobayashi,, Z. Hamir,, M. Jalbert,, and N. Moledina. 1993. Evaluation of a commercial enzyme immunoassay for the detection of a cryptococcal antigen. Mycoses 36:3134.
101. Shaunak, S.,, W. A. Schell,, and J. R. Perfect. 1989. Cryptococcal meningitis with normal cerebrospinal fluid. J. Infect. Dis. 160:912. (Letter.)
103. Snow, R. M.,, and W. E. Dismukes. 1975. Cryptococcal meningitis: diagnostic value of cryptococcal antigen in cerebrospinal fluid. Arch. Intern. Med. 135:11551157.
104. Speed, B. R.,, J. Kaldor,, B. Cairns,, and M. Pegorer. 1997. Serum antibody response to active infection with Cryptococcus neoformans and its varieties in immunocompetent subjects. J. Med. Vet. Mycol. 34:187193.
105. Staib, F.,, M. Seibold,, E. Antweiler,, and B. Frohlich. 1989. Staib agar supplemented with a triple antibiotic combination for the detection of Cryptococcus neoformans in clinical specimens. Mycoses 32:448454.
106. Staib, F.,, M. Seibold,, E. Antweiler,, B. Frohlich,, S. Weber,, and A. Blisse. 1987. The brown color effect (BCE) of Cryptococcus neoformans in the diagnosis, control and epidemiology of C. neoformans infections in AIDS patients. Zentralbl. Bakteriol. Hyg. A 266:167177.
107. Stamm, A. M.,, and S. S. Polt. 1980. False-negative cryptococcal antigen test. JAMA 244:1359.
107a. St.-Germain, G.,, and D. Beauchesne. 1991. Evaluation of the MicroScan Rapid Yeast Identification panel. J. Clin. Microbiol. 29:22962299.
108. Taelman, H.,, J. Bogaerts,, J. Batungwanayo,, P. Van de Perre,, S. Lucas,, and S. Allen. 1994. Failure of the cryptococcal serum antigen test to detect primary pulmonary cryptococcosis in patients infected with human immunodeficiency virus. Clin. Infect. Dis. 18:119120.
109. Tanner, D. C.,, M. P. Weinstein,, B. Fedorciw,, K. L. Joho,, J. J. Thorpe,, and L. B. Relier. 1994. Comparison of commercial kits for detection of cryptococcal antigen. J. Clin. Microbiol. 32:16801684.
110. Vartivarian, S. E.,, E. J. Anaissie,, R. E. Cowart,, H. A. Sprigg,, M. J. Tingler,, and E. S. Jacobson. 1993. Regulation of cryptococcal capsular polysaccharide by iron. J. Infect. Dis. 167:186190.
111. Vartivarian, S. E.,, R. E. Cowart,, E. J. Anaissie,, T. Tashiro,, and H. A. Sprigg. 1995. Iron acquisition by Cryptococcus neoformans. J. Med. Vet. Mycol. 33:151156.
112. Vilgalys, R.,, and M. Hester. 1990. Rapid genetic identification and mapping of enzy-matically amplified ribosomal DNA from several Cryptococcus species, J. Bacteriol. 172:42384246.
113. Vogel, R. A. 1966. The indirect fluorescent antibody test for the detection of antibody in human cryptococcal disease. J. Infect. Dis. 116:573580.
114. Walter, J. E.,, and R. D. Jones. 1968. Serodiagnosis of clinical cryptococcosis. Am. Reo. Respir. Dis. 97:275282.
115. Whittier, S.,, R. L. Hopfer,, and P. H. Gilligan. 1994. Elimination of false-positive serum reactivity in latex agglutination test for cryptococcal antigen in human immunodeficiency virus-infected population. J. Clin. Microbiol. 32:21582161.
116. Wickes, B. L.,, M. E. Mayorga,, U. Edman,, and J. C. Edman. 1996. Dimorphism and haploid fruiting in Cryptococcus neoformans association with the alpha-mating type. Proc. Natl. Acad. Sci. USA 93:73277331.
117. Wu, T.,, and S. Y. Koo. 1983. Comparison of three commercial cryptococcal latex kits for detection of cryptococcal antigen. J. Clin. Microbiol. 18:11271130.
118. Yagupsky, P.,, and M. A. Menegus. 1990. Cumulative positivity rates of multiple blood cultures for Mycobacterium avium intracellular and Cryptococcus neoformans in patients with the acquired immunodeficiency syndrome. Arch. Pathol. Lab. Med. 114:923925.
119. Yuen, C.,, A. Graziani,, N. Pietroski,, R. MacGregor,, and M. Schuster. 1994. Cryptococcal antigenemia in HIV-infected patients. Clin. Infect. Dis. 19:579.
120. Zerpa, R.,, L. Huicho,, and A. Guillen. 1996. Modified India ink preparation for Cryptococcus neoformans in cerebrospinal fluid specimens, J. Clin. Microbiol. 34:22902291.
121. Zimmer, B. L.,, and G. D. Roberts. 1979. Rapid selective urease test for presumptive identification of Cryptococcus neoformans. J. Clin. Microbiol. 10:380381.

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