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Chapter 5 : Molecular Biology

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Molecular Biology, Page 1 of 2

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Abstract:

The understanding of the epidemiology and pathogenic features for infections can now be aided by the use of molecular biological techniques. In the last three decades, model systems for eukaryotic molecular biology have developed around certain fungi, such as , , , and . In the late 1980s, studies for the present foundation of molecular biology in began. The early development of molecular biological techniques for studying a pathogen generally involves questions of epidemiology in relationship to pathogenicity. In fact, little work has yet been performed on the genetics and molecular biology of mitochondrial DNA in . The use of polymerase chain reaction (PCR) technology combined with repetitive primers (arbitrary primed PCR) has allowed investigators to identify the DNA fingerprints of different fungal strains rapidly, specifically, and with a minimal number of DNA purifications. Recent work in molecular phylogeny of has focused on other genes and their ancestral lineages. Investigations into gene structures and the understanding of gene regulation in this yeast are in their infancy.

Citation: Casadevall A, Perfect J. 1998. Molecular Biology, p 115-144. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch5

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Random Amplified Polymorphic DNA
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Restriction Fragment Length Polymorphism
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Figure 1

Fitch-Margoliash distance tree inferred from the more conservative 16S-like rRNA sequence matrix. (From reference with permission.)

Citation: Casadevall A, Perfect J. 1998. Molecular Biology, p 115-144. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch5
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Image of Figure 2
Figure 2

Fitch-Margoliash distance tree inferred from the more conservative 28S-like rRNA sequence matrix. (From reference with permission.)

Citation: Casadevall A, Perfect J. 1998. Molecular Biology, p 115-144. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch5
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References

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Tables

Generic image for table
Table 1

Cloned and identified genes of

Citation: Casadevall A, Perfect J. 1998. Molecular Biology, p 115-144. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch5
Generic image for table
Table 2

Strategies for gene isolations in

Citation: Casadevall A, Perfect J. 1998. Molecular Biology, p 115-144. In . ASM Press, Washington, DC. doi: 10.1128/9781555818241.ch5

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