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Chapter 7 : Koch's Role in the Microscope Revolution

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Abstract:

One of Robert Koch's main contributions was the successful adaptation of the light microscope to the study of bacteria, especially those found in diseased tissues. He was the first to use oil immersion lenses and the Abbe condenser, and he was the first to publish photomicrographs of bacteria. His research on the staining of bacteria for microscopy provided the foundation for this important topic. Much of his work was motivated by a desire to photograph bacteria through the microscope, as he realized that hand drawings were unsatisfactory for communicating the results of bacteriological investigations. His work on photomicroscopy not only forced him to improve his microscopy but also to perfect better methods of preparing specimens for microscopy. Initially, Koch used a vertical camera-microscope arrangement, as had been described by Reichardt and Sturenburg but this arrangement only permitted a magnification through the microscope of 300 X. Further magnification had to be made by enlargement of the negative, an unsatisfactory procedure. Later, Koch acquired a horizontal microscope-camera setup in which the camera, microscope, and mirror lighting arrangement were carefully aligned on an optical bench.

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7

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Figure 7.1

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.2

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.3

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.4

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.5

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.6

Spirillum UndulaSpirochaete ObermeieriBacillus anthracis

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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Figure 7.7

Citation: Brock T. 1999. Koch's Role in the Microscope Revolution, p 54-69. In Robert Koch. ASM Press, Washington, DC. doi: 10.1128/9781555818272.ch7
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