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Chapter 1 : Reprint of Temin and Rubin's 1958 Paper Describing the Focus Assay for Transformation by Rous Sarcoma Virus
Reprint of Temin and Rubin's 1958 Paper Describing the Focus Assay for Transformation by Rous Sarcoma Virus, Page 1 of 2< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555818302/9781555810986_Chap01-1.gif /docserver/preview/fulltext/10.1128/9781555818302/9781555810986_Chap01-2.gif
In 1958, Howard Temin and Harry Rubin developed the first quantitative assay for the transformation of cultured cells by tumor viruses. Prior to their work, Rous sarcoma virus had become the first generally accepted tumor virus because of its ability to cause sarcomas following inoculation of chickens. In order to address the fundamental questions of tumor virology, an in vitro assay that allowed virus-induced transformation to be analyzed and quantitated under controlled and reproducible experimental conditions was needed. This was provided by Temin and Rubin, whose assay for cell transformation by Rous sarcoma virus remains the standard assay used in studies of transformation induced not only by tumor viruses but also by chemicals, radiation, and isolated oncogenes of both viral and cellular origins. By permitting quantitative studies of virus replication, the focus assay provided the fundamental tool needed for analysis of the virus life cycle, including Temin’s subsequent experiments from which the provirus hypothesis was derived. The availability of the focus assay also allowed other workers to isolate Rous sarcoma mutants defective in transformation, leading to the identification of the src oncogene. The focus assay has similarly been applicable to studies not only of other tumor viruses but also of cellular oncogenes. Developed over 35 years ago, Howard Temin’s assay for Rous sarcoma virus thus remains a standard tool in virology and molecular oncology.
Key Concept Ranking
- Rous sarcoma virus