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Chapter 15 : Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation

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Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, Page 1 of 2

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Abstract:

For two decades, Howard Temin analyzed retroviral replication in detail in order to elucidate the high rate of genetic variation inherent to this process. In "Retrovirus Variation and Reverse Transcription: Abnormal Strand Transfers Result in Retrovirus Genetic Variation," he summarized his understanding of the kinds of retroviral variation and proposed a mechanism by which several of them might occur. Virologists have appreciated for many years that viruses with RNA genomes vary rapidly and that retroviruses do, too. One contribution to this high rate is thought to be inherent in retroviral RNA replicases: they lack the editing activities of DNA replicases, which serve to limit some types of errors potentially made by nucleic acid replicases. One elegant contribution to the resolution of the problem of multiple rounds of viral replication was the development and application of helper cells by Howard Temin and his colleagues. They developed cells that constitutively express the structural genes of spleen necrosis virus, a simple retrovirus. Howard, his students, and his postdoctoral researchers developed several protocols for selecting and enumerating the frequency of mutations generated in these viral vectors after one round of their replication. The identities of the mutants were revealed by sequencing their DNA after its rescue from the infected cells. Howard's hypothesis is appealing because it provides a single explanation for a variety of both simple and complex mutations known to arise in retroviruses. It also helps explain how this family of viruses can in general acquire cellular genes and in particular transduce oncogenes.

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, p 215-220. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch15

Key Concept Ranking

Genetic Elements
0.84199107
Genetic Variation
0.66697776
Reverse Transcription
0.56485945
Reverse Transcriptase
0.5439387
DNA
0.5084312
RNA Polymerase
0.49687502
0.84199107
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, p 215-220. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch15
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, p 215-220. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch15
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Figure 3

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, p 215-220. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint
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Figure 4

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1993 Paper on the Inherent Contributions of Reverse Transcription to Retroviral Variation, p 215-220. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint

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