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Chapter 2 : Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis

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Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, Page 1 of 2

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Abstract:

The development of the focus assay provided the means for studying both cell transformation and the replication of Rous sarcoma virus (RSV). As Howard Temin proceeded with these studies, he made a series of unexpected observations indicating that the replication of RSV was fundamentally different from that of other RNA-containing viruses. On the basis of these findings, he proposed the DNA provirus hypothesis, which stated that the viral RNA was copied into DNA in infected cells. The resulting DNA provirus was then replicated and stably inherited as part of the infected cell's genome. The DNA provirus hypothesis was based on several different types of experimental evidence, which are presented in the 1964 paper. Studies of cell transformation using mutants of RSV indicated that the morphology of transformed cells was determined by genetic information from the virus. On the basis of the observations, he proposed that the viral genome was present in infected cells in a stably inherited form, which he called a provirus. Evidence that the provirus is DNA was then derived from experiments with metabolic inhibitors. Temin sought further evidence for the proposal that the provirus was a DNA copy of the viral RNA genome, by using nucleic acid hybridization to detect viral sequences in infected-cell DNA, but the sensitivities of the available techniques were limited, and the data were unconvincing. The DNA provirus hypothesis was thus proposed on the basis of genetic experiments and the effects of metabolic inhibitors.

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2

Key Concept Ranking

Rous sarcoma virus
0.5555556
Viral RNA
0.46296296
DNA Synthesis
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0.5555556
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Figure 7

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Figure 10

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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Figure 14

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Temin's 1964 Paper Proposing the DNA Provirus Hypothesis, p 25-40. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch2
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