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Chapter 3 : Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light

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Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light, Page 1 of 2

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Abstract:

During the 1960s, Howard Temin pioneered different routes for testing his hypothesis of the DNA provirus. In the latter part of the decade, he and David Boettiger perfected an experimental protocol to test whether or not 5'-bromodeoxyuridine (5BUdR) was incorporated into retroviral replicative intermediates. This analog of thymidine had previously been shown to be incorporated into DNA, and being brominated, it rendered the DNA that contained it more sensitive to visible light than unhalogenated DNA would be. Boettiger and Temin induced chicken cells to become stationary by withdrawing serum from their cultures. These nonproliferating cells did not efficiently incorporate 5BUdR into their chromosomal DNA and thus were not sensitive to exposure to visible light. Temin and Boettiger infected the stationary cells, treated them with 5BUdR, exposed them to increasing doses of visible light, plated the treated chicken cells on a feeder layer of rat cells resistant to infection, and provided serum. This experiment, along with its described controls, indicated that in stationary cells, infecting retroviruses do synthesize DNA intermediates that can be tagged by the 5BUdR they incorporate. This finding was the most direct proof of the existence of a DNA provirus until that proviral DNA was isolated and detected 5 years later. This finding formed the basis for later studies in which Howard Temin and his colleagues explored the structure of the replicative intermediate and found that newly infected cells must pass through mitosis for infection to be completed.

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light, p 41-45. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch3

Key Concept Ranking

Chromosomal DNA
0.77072906
DNA Synthesis
0.5320089
Viral RNA
0.5263158
0.77072906
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Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light, p 41-45. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch3
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Image of Figure 2
Figure 2

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light, p 41-45. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch3
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Image of Figure 3
Figure 3

Citation: Cooper G, Temin R, Sugden B. 1995. Reprint of Boettiger and Temin's 1970 Paper on Inactivation of Bromodeoxyuridine-Containing Proviral DNA by Visible Light, p 41-45. In The DNA Provirus. ASM Press, Washington, DC. doi: 10.1128/9781555818302.ch3
Permissions and Reprints Request Permissions
Download as Powerpoint

References

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