Chapter 16 : Expression of the Uhp Sugar-Phosphate Transport System of

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $15.00

Preview this chapter:
Zoom in

Expression of the Uhp Sugar-Phosphate Transport System of , Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818319/9781555810894_Chap16-1.gif /docserver/preview/fulltext/10.1128/9781555818319/9781555810894_Chap16-2.gif


This chapter reviews the regulation of the Uhp system, which is a sugar-phosphate transport protein whose expression is induced by external glucose 6-phosphate (Glu6P). The presence of organophosphate transport systems in many gram-positive and gram-negative bacteria suggests that their organophosphate substrates are widely available. Several organophosphate transport systems have been analyzed in or . Expression of UhpT also occurs as part of the OxyR peroxide response system. As expected, loss of resulted in complete loss of detectable expression. The dominant negative behavior is explained if the truncated variants retain repression of but lack the ability to activate transcription of , analogous to the situation in LuxR. Linker substitution mutations in which the native sequences in the -64 region are converted to an I restriction site reduced or eliminated promoter function, depending on the location of the substitution and the number of base pair residues that were changed. Several surface-exposed regions of the catabolite gene activator protein (CAP) have been found to be necessary for transcription activation at CAP-dependent promoters that do not require the action of other transcription activator proteins. Sugar phosphates that are not directly metabolized by glycolysis also inhibit growth of those cells but do not elicit cell killing or methylglyoxal production.

Citation: Kadner R. 1995. Expression of the Uhp Sugar-Phosphate Transport System of , p 263-274. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch16

Key Concept Ranking

Transcription Start Site
Highlighted Text: Show | Hide
Loading full text...

Full text loading...


Image of FIGURE 1

Schematic model of Uhp components. The membrane-associated proteins are indicated with their predicted transmembrane topology. My results suggest that UhpB and UhpC act as a signaling complex, in which the binding o f Glu6P to UhpC affects the accessibility or activity of the kinase portion in the C-terminal half o f UhpB. We presume that phosphate transfer occurs from UhpB to Asp-54 in UhpA and that this phosphorylation allows UhpA to activate transcription at the promoter, in conjunction with RNA polymerase holoenzyme and the cyclic AMP-CAP complex. From , with permission.

Citation: Kadner R. 1995. Expression of the Uhp Sugar-Phosphate Transport System of , p 263-274. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch16
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 2

Schematic model of the location and phenotype of mutants in UhpB. The location of 12-bp linker insertions is indicated in rectangles, relative to the predicted transmembrane topology of the protein. Insertions conferred a range of regulatory phenotypes, including fully constitutive (open boxes), inducible behavior with elevated uninduced level (shaded boxes), or lack of expression (filled boxes). The location and sequence change of single base substitutions that allow Uhp expression in the absence of UhpC function are indicated in the C-terminal portion of UhpB.

Citation: Kadner R. 1995. Expression of the Uhp Sugar-Phosphate Transport System of , p 263-274. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch16
Permissions and Reprints Request Permissions
Download as Powerpoint


1. Ackerman, R. S.,, N. R. Cozzarelli,, and W. Epstein. 1974. Accumulation of toxic concentrations of methylglyoxal by wild-type Escherichia coliK-12. J. Bacteriol 119:357362.
2. Ambudkar, S. V.,, V. Anantharam,, and P. C. Maloney. 1990. UhpT, the sugar phosphate antiporter on Escherichia coli, functions as a monomer.J. Biol. Chem. 265:1228712292.
3. Atkinson, M. R.,, and A. J. Ninfa. 1993. Mutational analysis of the bacterial signal-transducing protein kinase/phosphatase nitrogen regulator II (NRn or NtrB) J. Bacteriol. 175:70167023.
4. Brzoska, P.,, M. Rimmele,, K. Brzostek,, and W. Boos. 1994. The pho regulon-dependent Ugp uptake system for glycerol-3-phosphate in Escherichia coli is trans inhibited by Pi J. Bacteriol. 176:1520.
5. Choi, S. H.,, and E. P. Greenberg. 1992. Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein. J. Bacteriol. 174:40644069.
6. Dietz, G. W. 1976. The hexose phosphate transport system of Escherichia coli. Adv. Enzymol. 44:237259.
7. Eiglmeier, K.,, W. Boos,, and S. Cole. 1987. Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system. Mol. Microbiol. 1:251258.
8. Friedrich, M. J.,, and R. J. Kadner. 1987. Nucleotide sequence of the uhp region of Escherichia coli. J. Bacteriol. 169:35563563.
9. Goldrick, D.,, G.-Q. Yu,, S.-Q. Jiang,, and J. - S. Hong. 1988. Nucleotide sequence and transcription start point of the phosphoglycerate transporter gene of Salmonella typhimurium. J. Bacteriol. 170: 34213426.
10. HenikofF, S.,, J. C. Wallace,, and J. P. Brown. 1990. Finding protein similarities with nucleotide sequence databases. Methods Enzymol. 183:111132.
11. Island, M. D.,, and R. J. Kadner. 1993. Interplay between the membrane-associated UhpB and UhpC regulatory proteins. J. Bacteriol. 174:50285034.
12. Island, M. D.,, B.- Y. Wei,, and R. J. Kadner. 1992. Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 174:27542762.
13. Kadner, R. J.,, M. D. Island,, T. J. Merkel,, and C. A. Webber,. 1994. Transmembrane control of the Uhp sugar-phosphate transport system: the sensation of Glu6R p. 7884. In A. Torriani-Gorini,, E. Yagil,, and S. Silver (ed.), Phosphate in Microorganisms: Cellular and Molecular Biology. American Society for Microbiology, Washington, D.C..
14. Kadner, R. J.,, G. R Murphy,, and C. M. Stephens. 1992. Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli. J. Gen. Microbiol. 138:20072014.
15. Kolb, A.,, S. Busby,, H. Buc,, S. Garges,, and S. Adhya. 1993. Transcriptional regulation by cAMP and its receptor protein. Annu. Rev. Biochem. 62:749795.
16. Maloney, P. C.,, S. V. Ambudkar,, V. Anantharam,, L. A. Sonna,, and A. Varadhachary. 1990. Anion-exchange mechanisms in bacteria. Microbiol. Rev. 54:117.
17. Merkel, T. J.,, J. L. Dahl,, R. H. Ebright,, and R. J. Kadner. 1995. Transcription activation at the Escherichia coli uhpT promoter by the catabolite gene activator protein J. Bacteriol. 177:17121718.
18. Merkel, T. J.,, and R. J. Kadner. Unpublished data.
19. Merkel, T. J.,, D. M. Nelson,, C. L. Brauer,, and R. J. Kadner. 1992. Promoter elements required for positive control of transcription of the Escherichia coli uhpTgene.J. Bacteriol. 174:27632770.
20. Parkinson, J. S.,, and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71112.
21. Pogell, B. M.,, B. R. Maity,, S. Frumkin,, and S. Shapiro. 1966. Induction of an active transport system for glucose-6-phosphate in Escherichia coli. Arch. Biochem. Biophys. 116:406415.
22. Pradel, E.,, and P. L. Boquet. 1989. Mapping of the Escherichia coli acid glucose-l-phosphatase gene agp and analysis of its expression in vivo by use of an agp-phoA protein fusion.J. Bacteriol. 171:35113517.
23. Scholten, M.,, and J. Tommassen. 1993. Topology of the PhoR protein of Escherichia coli and functional analysis of internal deletion mutants. Mol. Microbiol. 8:269275.
24. Schweizer, H.,, W. Boos,, and T. J. Larson. 1985. Repressor for the sn-glycerol-3-phosphate regulon of Escherichia coli K-12: cloning of the glpR gene and identification of its product. J. Bacteriol. 161:563566.
25. Shattuck-Eidens, D. M.,, and R. J. Kadner. 1981. Exogenous induction of the Escherichia coli hexose phosphate transport system defined by uhp-lac operon fusions.J. Bacteriol. 148:203209.
26. Shattuck-Eidens, D. M.,, and R. J. Kadner. 1983. Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli. J. Bacteriol. 155:10601070.
27. Sonna, L. A.,, S. V. Ambudkar,, and P. C. Maloney. 1988. The mechanism of glucose-6-phosphate transport by Escherichia coli. J. Biol. Chem. 263: 66256630.
28. Storz, G.,, and S. Altuvia. Personal communication.
29. Volz, K., 1993. Structural conservation in the CheY superfamily. Biochemistry 32:1174111753.
30. Wanner, B. L. 1993. Gene regulation by phosphate in enteric bacteria.J. Cell. Biochem. 51:4754.
31. Webber, C. A.,, and R. J. Kadner. Action of receiver and activator modules of UhpA in transcriptional control of the Escherichia coli sugar phosphate transport system. Mol. Microbiol. 15., in press.
32. Webber, C. A.,, and T. J. Merkel. Unpublished data.
33. Wei, B.-Y. Unpublished data.
34. Weston, L. A.,, and R. J. Kadner. 1988. Role of uhp genes in expression of the Escherichia coli sugarphosphate transport system. J. Bacteriol. 170:33753383.
35. Winkler, H. H. 1970. Compartmentation in the induction of the hexose-6-phosphate transport system of Escherichia coli. J. Bacteriol. 101:470475.
36. Yang, Y.-L.,, D. Goldrick,, and J.-S. Hong. 1988. Identification of the products and nucleotide sequence of two regulatory genes involved in exogenous induction of phosphoglycerate transport in Salmonella typhimurium. J. Bacteriol. 170:42994303.
37. Yu, G.-Q.,, and J. - S. Hong. 1986. Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium. Gene 45:5157.


Generic image for table

Uhp regulation in double linker insertion mutants

Citation: Kadner R. 1995. Expression of the Uhp Sugar-Phosphate Transport System of , p 263-274. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch16

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error