Chapter 19 : Two-Component Signal Transduction and Its Role in the Expression of Bacterial Virulence Factors

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In contrast to saprophytic bacteria, pathogenic bacteria live on or within living host tissues. Pathogenic bacteria coordinate an intricate network of virulence factors, whose expression must be precisely controlled to maximize the chance of establishing a successful infection. Certain common themes have emerged from the study of the control of bacterial virulence gene expression. has adapted the OmpR-EnvZ system, initially described in and also present in spp., for its own use to regulate expression of virulence factors and the OmpF and OmpC porins. An in vivo model for bacterial arthritis has indicated that AgrA may be involved in the direct regulation of additional genes whose expression is not mediated by RNA III. Bacteria deleted for the locus were unable to migrate through blood to the joint cavities and could not be recovered from these locations. Unlike wild-type , mutant bacteria could be recovered from the spleen, and one possible explanation is that the increased expression of cell wall-associated proteins in an mutant renders it more easily phagocytosed or immobilized in the reticulo-endothelial system. PilA and PilB are believed to control the expression of other genes as well, because two-dimensional gels of mutants showed significant differences when compared with wild-type cells.

Citation: Dziejman M, Mekalanos J. 1995. Two-Component Signal Transduction and Its Role in the Expression of Bacterial Virulence Factors, p 305-317. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch19

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1. Abdelnour, A.,, S. Arvidson,, T. Bremell,, C. Ryden,, and A. Tarkowski. 1993. The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine arthritis model. Infect. Immun. 61:38793885.
2. Bachmann, F. M. 1913. The migration of Bacillus amybvorus in the host tissues. Phytopathology 3:314.
3. Bartkus, J. M.,, and S. H. Leppla. 1989. Transcriptional regulation of the protective antigen gene of Bacillus anthracis. Infect. Immun. 57:22952300.
4. Bayles, K. W. 1993. The use of degenerate, sensor gene-specific, oligodeoxyribonucleotide primers to amplify DNA fragments from Staphylococcus aureus. Gene 123:99103.
5. Bender, R. A. 1991. The role of NAC protein in the nitrogen regulation of Klebsiella aerogenes. Mol. Microbiol. 5:25752580.
6. Bernardini, M. L.,, A. Fontaine, and R J. Sansonetti. 1990. The two-component regulatory system ompR-envZ controls the virulence of Shigella flexneri. J. Bacteriol. 172:62746281.
7. Bernardini, M. L.,, J. Mounier,, H. d'Hautevile,, M. Coquis-Rondon,, and P. J. Sansonetti. 1989. Identification of icsA, a plasmid locus of Shigella flexneri that governs intra- and inter-cellular spread through interaction with F-actin. Proc. Natl. Acad. Sci. USA 86:38673871.
8. Bernardini, M. L.,, M. G. Sanna,, A. Fontaine,, and P. Sansonetti. 1993. OmpC is involved in invasion of epithelial cells by Shigella flexneri. Infect. Immun. 61:36253635.
9. Boslego, J. W.,, E. C. Tramont,, R. C. Chung,, D. G. McChesney,, J. Ciak,, J. C. Sadoflf,, M. V. Piziak,, J. D. Brown,, C. J. Brinton,, S. W. Wood,, and J. R. Bryan. 1991. Efficacy trial of a parenteral gonococcal pilus vaccine in men. Vaccine 9:154162.
10. Caparon, M. G.,, R. T. Geist,, J. Perez-Casal,, and J. R. Scott. 1992. Environmental regulation of virulence in group A Streptococci: transcription of the gene encoding M protein is stimulated by carbon dioxide. J. Bacteriol. 174:56935701.
11. Collins, C. M.,, D. M. Gutman,, and H. Laman. 1993. Identification of a nitrogen-regulated promoter controlling expression of Klebsiella pneumoniae urease genes. Mol. Microbiol. 8:187198.
12. Coplin, D. L.,, K. Poetter,, and D. R. Majerczak. 1990. Regulation of exopolysaccharide synthesis in Erwinia stewartii by rcsB and rcsC. Phytopathology 81:1220.
13. Cornells, G.,, C. Sluiters,, C. L. de Rouvroit,, and T. Michiels. 1989. Homology between virF, the transcriptional activator of the Yersinia virulence regulon, and AraC, the Escherichia coli arabinose operon regulator J. Bacteriol. 171:254262.
14. Dale, J. B.,, and E. H. Beachey. 1985. Multiple, heart-cross-reactive epitopes of streptococcal M proteins. J. Exp. Med. 161:113122.
15. Dassy, B.,, T. Hogan,, T. J. Foster,, and J. Fournier. 1993. Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus. J. Gen. Microbiol. 139:13011306.
16. Dean, C. R.,, and K. Poole. 1993. Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas aeruginosa: involvement of a two-component regulatory system. Mol. Microbiol. 8:10951103.
17. Deutscher, J.,, and H. Sauerwald. 1986. Stimulation of dihydroxyacetone and glycerol kinase activity in Streptococcus faecalis by phosphoenolpyruvate-dependent phosphorylation catalyzed by enzyme I and HPr of the phosphotransferase system. J. Bacteriol. 166:829836.
18. Fischetti, V. A. 1989. Streptococcal M protein: molecular design and biological behavior. Clin. Microbiol. Rev. 2:285314.
19. Forsberg, A.,, A. M. Viitanen,, M. Skurnik,, and W. H. Wolf. 1991. The surface-located YopN protein is involved in calcium signal transduction in Yersinia pseudotuberculosis. Mol. Microbiol. 5:977986.
20. Friedrich, B.,, and B. Magasanik. 1977. Urease of Klebsiella aerogenes: control of its synthesis by glutamine synthetase.J. Bacteriol. 131:446452.
21. Fuqua, W. C.,, S. C. Winans,, and E. P. Greenberg. 1994. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.J. Bacteriol. 176:269275.
22. Galan, J. E.,, C. C. Ginocchio,, and P. Costeas. 1992. Molecular and functional characterization o f the Salmonella typhimurium invasion gene invA: homology of InvA to members of a new protein family.J. Bacteriol. 17:43384349.
23. Ginocchio, C. C.,, S. B. Olmsted,, C. L. Wells,, and J. E. Galan. 1994. Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium. Cell 76:717724.
24. Goldberg, M. B.,, O. Barzu,, C. Parsot,, and P. J. Sansonetti. 1993. Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement. J. Bacteriol. 175:21892196.
25. Granger, D. L.,, J. R. Perfect,, and D. T. Durack. 1985. Virulence o f Cryptococcus neqformans. Regulation of capsule synthesis by carbon dioxide. J. Clin. Invest. 76:508516.
26. Grimm, C.,, and N. J. Panopoulos. 1989. The predicted protein product of a pathogenicity locus from Pseudomonas syringae pv. phaseolicola is homologous to a highly conserved domain of several prokaryotic regulatory proteins.J. Bacteriol. 171: 50315038.
27. Hatheway, C. L. 1990. Toxigenic Clostridia. Clin. Microbiol. Rev. 3:6698.
28. Higgins, D. E.,, E. Nazareno,, and V. J. DiRita. 1992. The virulence gene activator ToxT from Vibrio cholerae is a member of the AraC family of transcriptional activators. J. Bacteriol. 174:68746980.
29. Hobbs, M.,, E. S. R. Collie,, P. D. Free,, S. P. Livingston,, and J. S. Mattick. 1993. PUS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 7:669682.
30. Imagawa, T.,, and Y. Higashi. 1992. An activity which restores theta toxin activity in some theta toxin-deficient mutants of Clostridium perfringens. Microbiol. Immunol. 36:523527.
31. Imagawa, T.,, T. Tatsuki,, T. Higashi,, and T. Amino. 1981. Complementation characteristics of newly isolated mutants from two groups of strains of Clostridium perfringens. Biken J. 24:1321.
32. Ishimoto, K. S.,, and S. Lory. 1992. Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene. J. Bacteriol. 174:35143521.
33. Janzon, L.,, and S. Arvidson. 1990. The role of the -lysin gene (htd) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus. EMBOJ. 9:13911399.
34. Janzon, L.,, S. Lofdahl,, and S. Arvidson. 1989. Identification and nucleotide sequence of the deltalysin gene, hid, adjacent to the accessory gene regulator (agr) in Staphylococcus aureus. Mol. Gen. Genet. 219:480485.
35. Kadurugamuwa, J. L.,, M. Rohde,, J. Wehland,, and K. N. Timmis. 1991. Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin. Infect. Immun. 59:34633471.
36. Keevil, C. W.,, N. C. Major,, D. B. Davies,, and A. Robinson. 1986. Physiology and virulence determinants of Neisseria gonorrhoeae grown in glucose-, oxygen- or cysteine-hmited continuous culture.J. Gen. Microbiol. 132:32893302.
37. Klaasen, P.,, and F. K. Graaf. 1990. Characterization of FapR, a positive regulator of expression of the 987P operon in enterotoxigenic Escherichia coli. Mol. Microbiol. 4:17791783.
38. Koehler, T. M.,, Z. Dai,, and M. Kaufman-Yarbray. 1994. Regulation of the Bacillus anthracis protective antigen gene: CO2 and a trans-acting element activate transcription from one or two promoters. J. Bacteriol. 176:586595.
39. Kofoid, E. C.,, and J. S. Parkinson. 1988. Transmitter and receiver modules in bacterial signaling proteins. Proc. Natl. Acad. Sci. USA 85:49814985.
40. Kornblum, J.,, B. N. Kreiswirth,, S. J. Projan,, H. Ross,, and R. P. Novick,. 1990. Agr: a polycistronic locus regulating exoprotein synthesis in Staphylococcus aureus, p. 373402. In R. Novick, and R. Skurray (ed.), The Molecular Biology of Staphylococci. VCH Publishers, New York.
41. Lancefield, R. C. 1962. Current knowledge of typespecific M antigens of group A streptococci. J. Immunol. 89:307313.
42. Leigh, J. A.,, and D. L. Coplin. 1992. Exopolysaccharides in plant-bacterial interactions. Annu. Rev. Microbiol. 46:307346.
43. Macaluso, A.,, E. A. Best,, and R. A. Bender. 1990. Role of the nac gene product in the nitrogen regulation of some NTR-regulated operons of Klebsiella aerogenes. J. Bacteriol. 172:72497255.
44. Makino, C.,, C. Sasakawa,, K. Kamata,, T. Kurata,, and M. Yoshikawa. 1986. A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in Shigella flexneri 2a. Cell 46:551555.
45. Makino, S. I.,, C. Sasakawa,, I. Uchida,, M. Terekado,, and M. Yoshikawa. 1988. Cloning and C02-dependent expression of the genetic region for encapsulation from Bacillus anthracis. Mol. Microbiol. 2:371376.
46. Mardh, P. A.,, and I. Westrom. 1976. Adherence of bacteria to vaginal epithelial cells. Infect. Immun. 13:661666.
47. McDonel, J. L. 1980. Clostridium perfringens toxins (type A, B, C, D, E). Pharmacol. Ther. 10:617635.
48. McGee, Z. A.,, A. P. Johnson,, and D. Taylor-Robinson. 1981. Pathogenic mechanisms of Neisseria gonorrhoeae: observation on damage to human fallopian tubes in organ culture by gonococci of colony type 1 or type A.J. Infect. Dis. 143:413422.
49. Mekalanos, J. J. 1992. Environmental signals controlling expression of virulence determinants in bacteria J. Bacteriol. 174:17.
50. Mills, S. D.,, C. A. Jasalavich,, and D. A. Cooksey. 1993. A two-component regulatory system required for copper-inducible expression of the copper resistance operon of Pseudomonas syringae. J. Bacteriol. 175:16561664.
51. Mizuta, K.,, M. Ohta,, M. Mori,, T. Hasagawa,, I. Nakashima,, and M. Kato. 1983. Virulence for mice of Klebsiella strains belonging to the 01 group: relationship to their capsular (K) types. Infect. Immun. 40:5661.
52. Morfeldt, E.,, L. Janzon,, S. Arvidson,, and L. Lofdahl. 1988. Cloning of a chromosomal locus (exp) which regulates the expression of several exoprotein genes. Mol. Gen. Genet. 211:435440.
53. Mounier, J.,, A. Ryter,, R. M. Coquis, and R J. Sansonetti. 1990. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocyte like cell line Caco-2. Infect. Immun. 58:10481058.
54. Mulrooney, S. B.,, H. S. Pankratz,, and R. P. Hausinger. 1989. Regulation of gene expression and cellular localization of cloned Klebsiella aerogenes (K. pneumoniae) urease. J. Gen. Microbiol. 135: 17691776.
55. Nixon, E. L. 1927. The migration of Bacillus amylovorus in apple tissue and its effects on host cells. Bull. Pennsylvania Agric. Exp. Station 212:316.
56. Okabe, A.,, T. Shimizu,, and H. Hayashi. 1989. Cloning and sequencing of a phospholipase C gene of Clostridium perfringens. Biochem. Biophys. Res. Commun. 160:3339.
57. Okada, M.,, R. T. Geist,, and M. G. Caparon. 1993. Positive transcriptional control of mry regulates virulence in the group A streptococcus. Mol. Microbiol. 7:893903.
58. Pace, J.,, M. J. Hayman,, and J. E. Galan. 1993. Signal transduction and invasion of epithelial cells by S. typhimurium. Cell 72:505514.
59. Peng, H. L.,, R. P. Novick,, B. Kreiswirth,, J. Kornblum,, and P. Schlievert. 1988. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. J. Bacteriol. 170:43654372.
60. Perez-Casal, J.,, M. G. Caparon,, and J. R. Scott. 1991. Mry, a frans-acting positive regulator of the M protein gene o f Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems.J. Bacteriol. 173:26172624.
61. Prevost, M. C.,, M. Lesourd,, M. Arplin, E Vernel, J. Mounier, R. Hellio, and P. J. Sansonetti. 1992. Unipolar reorganization of F-actin layer at bacterial division and bundling of actin filaments by plasrin correlate with movement of Shigella flexneri within HeLa cells. Infect. Immun. 60:40884099.
62. Regassa, L. B.,, and M. J. Betley. 1992. Alkaline pH decreases expression of the accessory gene regulator (agr) in Staphylococcus aureus. J. Bacteriol. 174:50955100.
63. Recsei, P.,, B. Kreiswirth,, M. O'Reilly,, P. Schlievert,, A. Gruss,, and R. Novick. 1986. Regulation of exoprotein gene expression by agr. Mol. Gen. Genet. 202:5861.
64. Roberts, I. S.,, and M. J. Coleman. 1991. The virulence of Erwinia amylovora: molecular genetic perspectives J. Gen. Microbiol. 137:14531457.
65. Robertson, E. S.,, J. C. Hoyt,, and H. C. Reeves. 1988. Evidence of histidine phosphorylation in isocitrate lyase from Escherichia coli. J. Biol. Chem. 263:24772482.
66. Rood, J. I.,, and S. T. Cole. 1991. Molecular genetics and pathogenesis of Clostridium perfringens. Microbiol. Rev. 55:621648.
67. Scott, J. R. 1990. The M Protein of Group A Streptococcus: Evolution and Regulation. Academic Press, San Diego, Calif.
68. Shimizu, T.,, W. Ba-thein,, M. Tamaki,, and H. Hayashi. 1994. The virR gene, a member o f a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens. J. Bacteriol. 176:16161623.
69. Shimizu, T.,, A. Okabe,, J. Min ami,, and H. Hayashi. 1991. An upstream regulatory sequence stimulates expression of the perfringolysin O gene of Clostridium perfringens. Infect. Immun. 59:137142.
70. Sidoti, C.,, G. Harwood,, R. Ackerman,, J. Coppard,, and M. Merrick. 1993. Characterisation of mutations in the Klebsiella pneumoniae nitrogen fixation regulatory gene nifL which impair oxygen regulation. Arch. Microbiol. 159:276281.
71. Sneath, B. J.,, X.-H. Jiang,, H. M. Howson,, and S. V. Beer. 1990. A pathogenicity gene from Erwinia amylovora encoded a predicted protein product homologous to a family of prokaryotic response regulators. Phytopathology 80:1038.
72. Stout, V.,, and S. Gottesman. 1990. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli. J. Bacteriol. 172:659669.
73. Stout, V.,, A. Torres-Cabassa,, M. R. Maurizi,, D. Gutnick,, and S. Gottesman. 1991. RcsA, an unstable positive regulator of capsular polysaccharide synthesis.J. Bacteriol. 173:17381747.
74. Swanson, J. 1973. Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells.J. Exp. Med. 137:571589.
75. Taha, M. K.,, B. Dupuy,, W. Saurin,, M. So,, and C. Marchal. 1991. Control of pilus expression in Neisseria gonorrhoeae as an original system in the family of two-component regulators. Mol. Microbiol. 5:137148.
76. Taha, M. K.,, M. Larribe,, B. Dupuy,, D. Giorgini,, and C. Marchal. 1992. Role of pilA, an essential regulatory gene of Neisseria gonorrhoeae, in the stress response..J. Bacteriol. 174:59785981.
77. Taha, M. K.,, M. So,, H. S. Seifert,, E. Billyard,, and C. Marchal. 1988. Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. EMBO J. 7:43674378.
78. Taha, M. L.,, and C. Marchal. 1990. Conservation of Neisseria gonorrhoeae pilus expression regulatory genes pilA and pilB in the genus Neisseria. Infect. Immun. 58:41454148.
79. Tilney, L. G.,, and D. A. Portnoy. 1989. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J. Cell Biol. 109:15971608.
80. Vandenesch, E.,, J. Kornblum,, and R. P. Novick. 1991. A temporal signal, independent of agr, is required for hla but not spa transcription in Staphylococcus aureus. J. Bacteriol. 173:63136320.
81. Wacharotayankun, R.,, Y. Arakawa,, M. Ohta,, K. Tanaka,, T. Akashi,, M. Mori,, and N. Kato. 1993. Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect. Immun. 61:31643174.
82. Youle, D.,, and R. M. Cooper. 1987. Possible determinants of pathogenicity of Erwinia amylovora, evidence for an induced toxin. Acta Hort. 217: 161167.


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Two-component regulation of virulence

Citation: Dziejman M, Mekalanos J. 1995. Two-Component Signal Transduction and Its Role in the Expression of Bacterial Virulence Factors, p 305-317. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch19
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Non-two-component regulation

Citation: Dziejman M, Mekalanos J. 1995. Two-Component Signal Transduction and Its Role in the Expression of Bacterial Virulence Factors, p 305-317. In Hoch J, Silhavy T (ed), Two-Component Signal Transduction. ASM Press, Washington, DC. doi: 10.1128/9781555818319.ch19

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