Chapter 3 : Early Events in Infection: Receptor Binding and Cell Entry

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As knowledge of the early events in infection with enteroviruses is limited, this chapter focuses on studies with poliovirus but examines the emerging data on several other enteroviruses. The early events in virus infection, from virus binding to uncoating of the viral genome, are well characterized for a number of enveloped viruses. The poliovirus-receptor interaction is a particularly good model for studying virus entry because of the experimental manipulations that are possible given the known structure of the virus and our ability to mutagenize both the virus and its cellular receptor. Despite our increasing genetic and structural understanding of early events in poliovirus infection, many problems, such as the location of the uncoating event, remain unsolved. Perhaps imminent studies on the entry of echoviruses (ECVs) and coxsackieviruses, stimulated by the identification of their receptors, will provide clues. The roles of receptors in host range and pathogenesis have been extensively studied for poliovirus, but many questions, such as the basis of tissue tropism, remain. A study of the cell functions of virus receptors may provide information on their role in virus replication. It has been suggested that virus binding to cell receptors may lead to activation of cell events that lead to disease, and there is evidence that receptors may regulate virus-induced cytopathic effects. Studies of the interactions of cell receptors with their natural cell ligands may therefore provide clues about cell processes that are activated upon virus binding and govern the outcome of virus infections.

Citation: Racaniello V. 1995. Early Events in Infection: Receptor Binding and Cell Entry, p 73-93. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch3

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Human immunodeficiency virus 1
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Herpes simplex virus 1
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Image of FIGURE 1

Locations of the VP 1 BC loop and mutations in the poliovirus capsid. The virion is shown schematically at left, with one protomer shaded gray. At right is an α-carbon tracing of VP1 and VP2 only. The BC loop is highlighted at the fivefold axis of symmetry. Sites of amino acid changes that confer the phenotype are shown as dots, and the amino acid residues are indicated. Sphingosine in the hydrocarbon-binding pocket is shown in dark gray.

Citation: Racaniello V. 1995. Early Events in Infection: Receptor Binding and Cell Entry, p 73-93. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch3
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Image of FIGURE 2

Structural model of the first Ig-like domain of PVR. The structure was predicted as described in reference . The β strands are lettered, and locations of mutations that affect virus binding are given as amino acid numbers, which begin with the first methionine of PVR ( ). This predicted structure differs slightly from two other published versions ( ).

Citation: Racaniello V. 1995. Early Events in Infection: Receptor Binding and Cell Entry, p 73-93. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch3
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Image of FIGURE 3

Model for poliovirus entry into cells. Binding of virus to cells is reversible when carried out at temperatures below 33°C; at higher temperatures, bound viruses are converted to A particles. These particles are found both outside and inside the cell and may be intermediates in the uncoating process. Uncoating may occur at the cell surface or from within endosomes.

Citation: Racaniello V. 1995. Early Events in Infection: Receptor Binding and Cell Entry, p 73-93. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch3
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1. Agol, V. I.,, S. G. Drozdov,, T. A. Ivannicova,, M. S. Kolesnikova,, M. B. Korolev,, and E. A. Tolskaya. 1989. Restricted growth of attenuated poliovirus strains in cultured cells of a human neuroblastoma. J. Virol. 63:40354038.
2. Aoki, J.,, S. Koike,, I. Ise,, Y. Sato-Yoshida,, and A. Nomoto. 1994. Amino acid residues on human poliovirus receptor involved in interaction with poliovirus. J. Biol. Chem. 269:84318438.
3. Armstrong, C. 1939. Successful transfer of the Lansing strain of poliomyelitis virus from the cotton rat to the white mouse. Public Health Rep. 54:23022305.
4. Badger, J.,, I. Minor,, M. J. Kremer,, M. A. Oliveira,, T. J. Smith,, J. P. Griffith,, D. M. A. Guerin,, S. Krishnaswamy,, M. Luo,, M. G. Rossmann,, M. A. McKinlay,, G. D. Diana,, F. J. Dutko,, M. Fancher,, R. R. Rueckert,, and B. A. Heinz. 1988. Structural analysis of a series of antiviral agents complexed with human rhinovirus 14. Proc. Natl. Acad. Sci. USA 85:33043308.
5. Bergelson, J. M.,, B. Chan,, R. W. Finberg,, and M. E. Hemler. 1993. The integrin VLA-2 binds echovirus 1 and extracellular matrix ligands by different mechanisms. J. Clin. Invest. 92:232239.
6. Bergelson, J. M.,, M. Chan,, K. R. Solomon,, N. F. St. John,, H. Lin,, and R. W. Finberg. 1994. Decay-accelerating factor (CD55), a glycosylphosphatidylinositol-anchored complement regulatory protein, is a receptor for several echoviruses. Proc. Natl. Acad. Sci. USA 91:62456248.
7. Bergelson, J. M.,, M. P. Shepley,, B. M. C. Chan,, M. E. Hemler,, and R. W. Finberg. 1992. Identification of the integrin VLA-2 as a receptor for echovirus 1. Science 255:17181720.
8. Bergelson, J. M.,, N. St. John,, S. Kawaguchi,, M. Chan,, H. Stubdal,, J. Modlin,, and R. W. Finberg. 1993. Infection by echoviruses 1 and 8 depends on the alpha2 subunit of human VLA-2. J. Virol. 67:68476852.
9. Bernhardt, G.,, J. A. Bibb,, J. Bradley,, and E. Wimmer. 1994. Molecular characterization of the cellular receptor for poliovirus. Virology 199:105113.
10. Bernhardt, G.,, J. Harber,, A. Zibert,, M. deCrombrugghe,, and E. Wimmer. 1994. The poliovirus receptor: identification of domains and amino acid residues critical for virus binding. Virology 203:344356.
11. Bibb, J. A.,, G. Bernhardt,, and E. Wimmer. 1994. The human poliovirus receptor alpha is a serine phosphoprotein. J. Virol. 68:61116115.
12. Bodian, D., 1959. Poliomyelitis: pathogenesis and histopathology, p. 479498. In T. M. Rivers, and F. L. Horsfall (ed.), Viral and Rickettsial Infections of Man. J. B. Lippincott, Philadelphia.
13. Brown, R. H.,, D. Johnson,, M. Ogonowski,, and H. L. Weiner. 1987. Type 1 human poliovirus binds to human synaptosomes. Ann. Neurol. 21:6470.
14. Campbell, B. A.,, and C. E. Cords. 1983. Monoclonal antibodies that inhibit attachment of group B coxsackieviruses. J. Virol. 48:561564.
15. Chang, K. H.,, C. Day,, J. Walker,, T. Hyypiä,, and G. Stanway. 1992. The nucleotide sequences of wild-type coxsackievirus A9 strains imply that an RGD motif in VP1 is functionally significant. J. Gen. Virol. 73:621626.
16. Chow, M.,, J. F. E. Newman,, D. Filman,, J. M. Hogle,, D. J. Rowlands,, and F. Brown. 1987. Myristylation of picornavirus capsid protein VP4 and its structural significance. Nature (London) 327:482486.
17. Colonno, R. J.,, P. L. Callahan,, and W. L. Long. 1986. Isolation of a monoclonal antibody that blocks attachment of the major group of human rhinoviruses. J. Virol. 57:712.
18. Colston, E.,, and V. R. Racaniello. Poliovirus variants selected on mutant receptor-expressing cells identify capsid residues that expand receptor recognition. Submitted for publication.
19. Colston, E.,, and V. R. Racaniello. Soluble receptor-resistant poliovirus mutants identify surface and internal capsid residues that control interaction with the cell receptor. EMBO J., in press.
20. Couderc, T.,, J. Hogle,, H. Le Blay,, F. Horaud,, and B. Blondel. 1993. Molecular characterization of mouse-virulent poliovirus type 1 Mahoney mutants: involvement of residues of polypeptides VP1 and VP2 located on the inner surface of the capsid protein shell. J. Virol. 67:38083817.
21. Couderc, T.,, A. Martin,, C. Wychowski,, M. Girard,, F. Horaud,, and R. Crainic. 1991. Analysis of neutralization-escape mutants selected from a mouse virulent type 1/type 2 chimeric poliovirus: identification of a type 1 poliovirus with antigenic site 1 deleted. J. Gen. Virol. 72:973977.
22. Crowell, R. L. 1963. Specific viral interference in HeLa cell cultures chronically infected with coxsackie B5 virus. J. Bacteriol. 86:517526.
23. Crowell, R. L.,, A. K. Field,, W. A. Schleif,, W. L. Long,, R. J. Colonno,, J. E. Mapoles,, and E. A. Emini. 1986. Monoclonal antibody that inhibits infection of HeLa and rhabdomyosarcoma cells by selected enteroviruses through receptor blockade. J. Virol. 57:438445.
24. Crowell, R. L.,, and B. J. Landau,. 1983. Receptors in the initiation of picornavirus infections, p. 142. In H. Fraenkel-Conrat, and R. R. Wagner (ed.), Comprehensive Virology. Academic Press, Inc., New York.
25. de Verdugo, U. R.,, H.-C. Selinka,, M. Huber,, P. H. Hofschneider,, and R. Kandolf. 1994. Purification and characterization of a specific attachment protein for coxsackie B viruses, abstr. 5, p. 5. Abstr. Europic ‘94.
26. Evans, C. A.,, P. H. Byatt,, V. C. Chambers,, and W. M. Smith. 1954. Growth of neurotropic viruses in extraneural tissues.VI. Absence of in vivo multiplication of poliomyelitis virus, types I and II, after intratesticular inoculation of monkeys and other animals. J. Immunol. 72:348352.
27. Filman, D. J.,, R. Syed,, M. Chow,, A. J. Macadam,, P. D. Minor,, and J. M. Hogle. 1989. Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO J. 8:15671579.
28. Fox, M. P.,, M. J. Otto,, and M. A. McKinlay. 1986. Prevention of rhinovirus and poliovirus uncoating by WIN 51711, a new antiviral drug. Antimicrob. Agents Chemother. 30:110116.
29. Freistadt, M. F.,, G. Kaplan,, and V. R. Racaniello. 1990. Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues. Mol. Cell. Biol. 10:57005706.
30. Freistadt, M. S.,, H. B. Fleit,, and E. Wimmer. 1993. Poliovirus receptor on human blood cells: a possible extraneural site of poliovirus replication. Virology 195:798803.
31. Fricks, C. E.,, and J. M. Hogle. 1990. The cell-induced conformational change of poliovirus: externalization of the amino terminus of VP1 is responsible for liposome binding. J. Virol. 64:19341945.
32. Gromeier, M.,, and K. Wetz. 1990. Kinetics of poliovirus uncoating in HeLa cells in a nonacidic environment. Virology 64:35903597.
33. Hogle, J. M.,, M. Chow,, and D. J. Filman. 1985. Three-dimensional structure of poliovirus at 2.9 Å resolution. Science 229:13581365.
34. Holland, J. J. 1961. Receptor affinities as major determinants of enterovirus tissue tropisms in humans. Virology 15:312326.
35. Holland, J. J.,, and B. H. Hoyer. 1962. Early stages of enterovirus infection. Cold Spring Harbor Symp. Quant. Biol. 27:101111.
36. Holland, J. J.,, J. C. McLaren,, and J. T. Syverton. 1959. The mammalian cell virus relationship. III. Production of infectious poliovirus by non-primate cells exposed to poliovirus ribonucleic acid. Proc. Soc. Exp. Biol. Med. 100:843845.
37. Holland, J. J.,, J. C. McLaren,, and J. T. Syverton. 1959. The mammalian cell virus relationship. IV Infection of naturally insusceptible cells with enterovirus ribonucleic acid. J. Exp. Med. 110:6580.
38. Hynes, R. O. 1992. Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:1122.
39. Hyypia, T.,, C. Horsnell,, M. Maaronen,, M. Khan,, N. Kalkkinen,, P. Auvinen,, L. Kinnunen,, and G. Stanway. 1992. A distinct picornavirus group identified by sequence analysis. Proc. Natl. Acad. Sci. USA 89:88478851.
40. Incardona, N. L.,, and P. Kaesberg. 1974. A pH-induced structural change in bromegrass mosaic virus. Biophys. J. 4:1121.
41. Joklik, W. K.,, and J. E. Darnell. 1961. The absorption and early fate of purified poliovirus in HeLa cells. Virology 13:439447.
42. Jubelt, B.,, B. Gallez-Hawkins,, O. Narayan,, and R. T. Johnson. 1980. Pathogenesis of human poliovirus infection in mice. I. Clinical and pathological studies. J. Neuropathol. Exp. Neurol. 39:138148.
43. Jubelt, B.,, O. Narayan,, and R. T. Johnson. 1980. Pathogenesis of human poliovirus infection in mice. II. Age-dependency of paralysis. J. Neuropathol. Exp. Neurol. 39:149158.
44. Kaplan, A. S. 1955. Comparison of susceptible and resistant cells to infection with poliomyelitis virus. Ann. N.Y. Acad. Sci. 61:830839.
45. Kaplan, G.,, M. S. Freistadt,, and V. R. Racaniello. 1990. Neutralization of poliovirus by cell receptors expressed in insect cells. J. Virol. 64:46974702.
46. Kaplan, G.,, D. Peters,, and V. R. Racaniello. 1990. Poliovirus mutants resistant to neutralization with soluble cell receptors. Science 250:15961599.
47. Kirkegaard, K. 1990. Mutations in VP1 of poliovirus specifically affect both encapsidation and release of viral RNA. J. Virol. 64:195206.
48. Koike, S.,, H. Horie,, I. Dise,, H. Okitsu,, M. Yoshida,, N. Iizuka,, K. Takeuthi,, T. Takegami,, and A. Nomoto. 1990. The poliovirus receptor protein is produced both as membrane-bound and secreted forms. EMBO J. 9:32173224.
49. Koike, S.,, I. Ise,, and A. Nomoto. 1991. Functional domains of the poliovirus receptor. Proc. Natl. Acad. Sci. USA 88:41044108.
50. Koike, S.,, I. Ise,, Y. Sato,, H. Yonekawa,, O. Gotoh,, and A. Nomoto. 1992. A second gene for the African green monkey poliovirus receptor that has no putative N-glycosylation site in the functional N-terminal immunoglobulin-like domain. J. Virol. 66:70597066.
51. Koike, S.,, C. Taya,, T. Kurata,, S. Abe,, I. Ise,, H. Yonekawa,, and A. Nomoto. 1991. Transgenic mice susceptible to poliovirus. Proc. Natl. Acad. Sci. USA. 88:951955.
52. Krah, D. L.,, and R. L. Crowell. 1982. A solid-phase assay of solubilized HeLa cell membrane receptors for binding group B coxsackieviruses and polioviruses. Virology 118: 148156.
53. Krah, D. L.,, and R. L. Crowell. 1985. Properties of the deoxycholate-solubilized HeLa cell plasma membrane receptor for binding group B coxsackieviruses. J. Virol. 53:867870.
54. Kunin, C. M. 1962. Virus-tissue union and the pathogenesis of enterovirus infections. J. Immunol. 8:556559.
55. Kunin, C. M.,, and W. S. Jordan. 1961. In vitro adsorption of poliovirus by noncultured tissues. Effect of species, age and malignancy. Am. J. Hyg. 73:245257.
56. Ledinko, N.,, J. T. Riordan,, and J. L. Melnick. 1951. Differences in cellular pathogenicity of two immunologically related poliomyelitis viruses as revealed in tissue culture. Proc. Soc. Exp. Biol. Med. 78:8388.
57. Li, Q.,, A. G. Yafal,, Y. H. Lee,, J. Hogle,, and M. Chow. 1994. Poliovirus neutralization by antibodies to internal epitopes of VP4 and VP1 results from reversible exposure of these sequences at physiological temperature. J. Virol. 68:39653970.
58. Lindberg, A. M.,, R. L. Crowell,, R. Zell,, R. Kandolf,, and U. Pettersson. 1992. Mapping of the RD phenotype of the Nancy strain of coxsackievirus B3. Virus Res. 24:187196.
59. Lloyd, R. E.,, and M. Bovee. 1993. Persistent infection of human erythroblastoid cells by poliovirus. Virology 194:200209.
60. Lonberg-Holm, K.,, R. L. Crowell,, and L. Philipson. 1976. Unrelated animal viruses share receptors. Nature (London) 259:679681.
61. Lonberg-Holm, K.,, and L. Philipson. 1974. Early interaction between animal viruses and cells. Monogr. Virol. 9:1148.
62. Lublin, D.,, and J. Atkinson. 1989. Decay-accelerating factor: biochemistry, molecular biology, and function. Annu. Rev. Immunol. 7:3557.
63. Macadam, A. J.,, G. Ferguson,, A. Nomoto,, and P. D. Minor. 1994. Sequences of the cellular receptor and viral capsid proteins influence the cytopathic effect of poliovirus, abstr. A2, p. 2. Abstr. Europic ‘94.
64. Madshus, I. H.,, S. Olsnes,, and K. Sandvig. 1984. Mechanism of entry into the cytosol of poliovirus type 1: requirement for low pH. J. Cell Biol. 98:11941200.
65. Madshus, I. H.,, S. Olsnes,, and K. Sandvig. 1984. Requirements for entry of poliovirus RNA into cells at low pH. EMBO J. 3:19451950.
66. Marsh, M.,, and A. Helenius. 1989. Virus entry into animal cells. Adv. Virus Res. 36:107151.
67. Martin, A.,, C. Wychowski,, T. Couderc,, R. Crainic,, J. Hogle,, and M. Girard. 1988. Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO J. 7:28392847.
68. Mason, P. W.,, B. Baxt,, F. Brown,, J. Harber,, A. Murdin,, and E. Wimmer. 1993. Antibody-complexed foot-and-mouth disease virus, but not poliovirus, can infect normally insusceptible cells via the Fc receptor. Virology 192:568577.
69. Mbida, A. D.,, O. G. Gaudin,, O. Sabido,, B. Pozzetto,, and J.-C. Le Bihan. 1992. Monoclonal antibody specific for the cellular receptor of echoviruses. Intervirology 33:1722.
70. Mbida, A. D.,, B. Pozzetto,, O. G. Gaudin,, F. Grattard,, J.-C. Le Bihan,, Y. Akono,, and A. Ros. 1992. A 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible cells. Virology 189:350353.
71. Mbida, A. D.,, B. Pozzetto,, O. Sabido,, Y. Akono,, F. Grattard,, M. Habib,, and O. G. Gaudin. 1991. Competition binding studies with biotinylated echovirus 11 in cytofluorimetry analysis. J. Virol. Methods 35:169176.
72. McLaren, L. C.,, J. J. Holland,, and J. T. Syverton. 1959. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J. Exp. Med. 109:475485.
73. McLaren, L. C.,, J. J. Holland,, and J. T. Syverton. 1960. The mammalian cell-virus relationship. V. Susceptibility and resistance of cells in vitro to infection by coxsackie A9 virus. J. Exp. Med. 112:581594.
74. Melnick, J. L., 1990. Enteroviruses: polioviruses, coxsackieviruses, echoviruses and newer enteroviruses, p. 549605. In B. N. Fields,, D. M. Knipe,, R. M. Chanock,, M. S. Hirsch,, J. L. Melnick,, T. P. Monath,, and B. Roizman (ed.), Virology. Raven Press, New York.
75. Mendelsohn, C.,, B. Johnson,, K. A. Lionetti,, P. Nobis,, E. Wimmer,, and V. R. Racaniello. 1986. Transformation of a human poliovirus receptor gene into mouse cells. Proc. Natl. Acad. Sci. USA 83:78457849.
76. Mendelsohn, C.,, E. Wimmer,, and V. R. Racaniello. 1989. Cellular receptor for poliovirus: molecular cloning, nucleotide sequence and expression of a new member of the immunoglobulin superfamily. Cell 56:855865.
77. Miller, D. A.,, O. J. Miller,, V. G. Dev,, S. Hashmi,, R. Tantravahi,, L. Medrano,, and H. Green. 1974. Human chromosome 19 carries a poliovirus receptor gene. Cell 1:167173.
78. Minor, P. D.,, P. A. Pipkin,, D. Hockley,, G. C. Schild,, and J. W. Almond. 1984. Monoclonal antibodies which block cellular receptors of poliovirus. Virus Res. 1:203212.
79. Morrison, M. E.,, and V. R. Racaniello. 1992. Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene. J. Virol. 66:28072813.
80. Morrison, M. E.,, H. Yuan-Jing,, M. W. Wien,, J. W. Hogle,, and V. R. Racaniello. 1994. Homolog scanning mutagenesis reveals poliovirus receptor residues important for virus binding and replication. J. Virol. 68: 25782588.
81. Moscufo, N.,, A. G. Yafal,, A. Rogove,, J. Hogle,, and M. Chow. 1993. A mutation in VP4 defines a new step in the late stages of cell entry by poliovirus. J. Virol. 67:50755078.
82. Moss, E. G.,, R. E. O’Neill,, and V. R. Racaniello. 1989. Mapping of attenuating sequences of an avirulent poliovirus type 2 strain. J. Virol. 63:18841890.
83. Moss, E. G.,, and V. R. Racaniello. 1991. Host range determinants located on the interior of the poliovirus capsid. EMBO J. 5:10671074.
84. Mosser, A. G.,, and R. R. Rueckert. 1993. WIN 51711-dependent mutants of poliovirus type 3: evidence that virions decay after release from cells unless drug is present. J. Virol. 67:12461254.
85. Moyer, A. Q.,, C. Accorti,, and H. R. Cox. 1952. Poliomyelitis I. Propagation of the MEF1 strain of poliomyelitis virus in the suckling hamster. Proc. Soc. Exp. Biol. Med. 81:513518.
86. Murray, M. G.,, J. Bradley,, X. F. Yang,, E. Wimmer,, E. G. Moss,, and V. R. Racaniello. 1988. Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I. Science 241: 213215.
87. Nobis, P.,, R. Zibirre,, G. Meyer,, J. Kuhne,, G. Warnecke,, and G. Koch. 1985. Production of a monoclonal antibody against an epitope on HeLa cells that is the functional poliovirus binding site. J. Gen. Virol. 6:25632569.
88. Olson, N. H.,, P. R. Kolatkar,, M. A. Oliveira,, R. H. Cheng,, J. M. Greve,, A. McClelland,, T. S. Baker,, and M. G. Rossmann. 1993. Structure of a human rhi-novirus complexed with its receptor molecule. Proc. Natl. Acad. Sci. USA 90:507511.
89. Pérez, L.,, and L. Carrasco. 1993. Entry of poliovirus into cells does not require a low-pH step. J. Virol. 67:45434548.
90. Racaniello, V. R.,, and D. Baltimore. 1981. Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916919.
91. Reagan, K. J.,, B. Goldberg,, and R. L. Crowell. 1984. Altered receptor specificity of coxsackievirus B3 after growth in rhabdomyosarcoma cells. J. Virol. 49:635640.
92. Ren, R.,, F. C. Costantini,, E. J. Gorgacz,, J. J. Lee,, and V. R. Racaniello. 1990. Transgenic mice expressing a human poliovirus receptor: a new model for poliomyelitis. Cell 63:353362.
93. Ren, R.,, and V. Racaniello. 1992. Human poliovirus receptor gene expression and poliovirus tissue tropism in transgenic mice. J Virol. 66:296304.
94. Roca-Garcia, M.,, A. W. Moyer,, and H. R. Cox. 1952. Poliomyelitis. II. Propagation of MEF1 strain of poliomyelitis virus in developing chick embryo by yolk sac inoculation. Proc. Soc. Exp. Biol. Med. 81:519525.
95. Roivainen, M.,, T. Hyypiä,, L. Piirainen,, N. Kalkkinen,, G. Stanway,, and T. Hovi. 1991. RGD-dependent entry of coxsackievirus A9 into host cells and its bypass after cleavage of VP1 protein by intestinal proteases. J. Virol. 65:47354740.
96. Roivainen, M.,, L. Piirainen,, T. Hyypiä,, I. Virtanen,, and T. Hovi. 1994. Receptors for coxsackievirus A9, abstr. A7, p. 7. Abstr. Europic ‘94.
97. Rombaut, B.,, P. Brioen,, and A. Boeyé. 1990. Disoxaril stabilization and immunogenic-ity of poliovirus procapsids. J. Gen. Virol. 71:10811086.
98. Rossmann, M. G.,, E. Arnold,, J. W. Erickson,, E. A. Frankenberger,, J. P. Griffith,, H.-J. Hecht,, J. E. Johnson,, and G. Kamer. 1985. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature (London) 317: 145153.
99. Ryu, S.,, P. D. Kwong,, A. Truneh,, T. G. Porter,, J. Arthos,, M. Rosenberg,, X. Dai,, N. Xuong,, R. Axel,, R. W. Sweet,, and W. A. Hendrickson. 1990. Crystal structure of an HIV-binding recombinant fragment of human CD4. Nature (London) 348:419426.
100. Selinka, H.-C.,, A. Zibert,, and E. Wimmer. 1991. Poliovirus can enter and infect mammalian cells by way of an intercellular adhesion molecule 1 pathway. Proc. Natl. Acad. Sci. USA 88:35983602.
101. Selinka, H.-C.,, A. Zibert,, and E. Wimmer. 1992. A chimeric poliovirus/CD4 receptor confers susceptibility to poliovirus on mouse cells. J. Virol. 66:25232526.
102. Shepard, D. A.,, B. A. Heinz,, and R. R. Rueckert. 1993. WIN 52035-2 inhibits both attachment and eclipse of human rhinovirus 14. J. Virol. 67:22452254.
103. Shepley, M. P.,, and V. R. Racaniello. 1994. A monoclonal antibody that blocks poliovirus attachment recognizes the lymphocyte homing receptor CD44. J. Virol. 68:13011308.
104. Shepley, M. P.,, B. Sherry,, and H. L. Weiner. 1988. Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. Proc. Natl. Acad. Sci. USA 85:77437747.
105. Smith, T. J.,, M. J. Kremer,, M. Luo,, G. Vriend,, E. Arnold,, G. Kamer,, M. G. Rossmann,, M. A. McKinlay,, G. D. Diana,, and M.J. Otto. 1986. The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. Science 233:12861293.
106. Stamenkovic, I.,, M. Amiot,, M. Pesando,, and B. Seed. 1989. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Cell 56:10571062.
107. Vile, R. G.,, and R.A. Weiss. 1991.Virus receptors as permeases. Nature (London) 352:666667.
108. Wetz, K.,, and T. Kucinski. 1991. Influence of different ionic and pH environments on structural alteration of poliovirus and their possible relation to virus uncoating. J. Gen. Virol. 72:25412544.
109. Willems, F. T. C.,, J. L. Melnick,, and W. E. Rawls. 1969. Replication of poliovirus in phytohemagglutinin-stimulated human lymphocytes. J. Virol. 3:451457.
110. Williams, A. F.,, and A. N. Barclay. 1988. The immunoglobulin superfamily—domains for cell surface recognition. Annu. Rev. Immunol. 6:381405.
111. Willingmann, P.,, H. Barnert,, H. Zeichhardt,, and K. O. Habermehl. 1989. Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis. Virology 168: 417420.
112. Yeates, T. O.,, D. H. Jacobson,, A. Martin,, C. Wychowski,, M. Girard,, D. J. Filman,, and J. M. Hogle. 1991. Three-dimensional structure of a mouse-adapted type 2/type 1 poliovirus chimera. EMBO J. 10:23312341.
113. Zajac, I.,, and R. Crowell. 1965. Location and regeneration of enterovirus receptors of HeLa cells. J. Bacteriol. 89:10971100.
114. Zajac, I.,, and R. L. Crowell. 1965. Effect of enzymes on the interaction of enteroviruses with living HeLa cells. J. Bacteriol. 89:574582.
115. Zeichhardt, H.,, K. Wetz,, P. Willingmann,, and K.-O. Habermehl. 1985. Entry of poliovirus type 1 and mouse Elberfeld (ME) virus into Hep2 cells: receptor-mediated endocytosis and endosomal or lysosomal uncoating. J. Gen. Virol. 66:483492.
116. Zhou, D. F. H.,, J. F. Ding,, L. J. Picker,, R. F. Bargatze,, E. C. Butcher,, and D. F. Goeddel. 1989. Molecular cloning and expression of Pgp-1. The mouse homologue of the human H-CAM (Hermes) lymphocyte homing receptor. J. Immunol. 143:33903395.
117. Zibert, A.,, H.-C. Selinka,, O. Elroy-Stern,, B. Moss,, and E. Wimmer. 1991. Vaccinia virus-mediated expression and identification of the human poliovirus receptor. Virology 182:250259.
118. Zibert, A.,, and E. Wimmer. 1992. N glycosylation of the virus binding domain is not essential for function of the human poliovirus receptor. J. Virol. 66:73687373.

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