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Chapter 4 : Viral RNA Synthesis

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Abstract:

This chapter summarizes what is known about how a single viral RNA molecule can be selectively amplified into thousands of RNA progeny in infected cells. It specifically provides the roles of viral proteins and RNA sequences in RNA replication, and describes the kinetics and products of RNA replication in infected cells. Next, it explains the sites and compositions of viral replication complexes (RCs) in infected cells. Then, the chapter discusses the models that have been proposed to explain how viral positive and negative RNA species are made by the viral RNA-dependent RNA polymerase. Finally, it describes the coupling between translation and replication processes in infected cells. Poliovirus is used as the prototype of an enterovirus because most of the research has been performed with poliovirus infected cell. To accomplish the unique task of RNA-dependent RNA polymerization in infected cells, enteroviruses encode several proteins required for viral RNA synthesis. Open questions about the mechanism of viral synthesis include the nature o f the RNA primers for positive- and negative-strand RNA synthesis, the source of specificity for the viral template RNA, and the relationship between translation and RNA synthesis, which may occur simultaneously in the infected host cell cytoplasm. Some of these questions may be studied with the recently discovered cellfree system.

Citation: Johnson K, Sarnow P. 1995. Viral RNA Synthesis, p 95-112. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch4

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Image of FIGURE 1
FIGURE 1

Genome organization of poliovirus. For viral RNA, the positive-strand RNA genome is shown. The presence of 3B (VPg) and polyadenosine sequences (An) at the 5′ end and 3′ ends, respectively, are indicated. The coding region is indicated by the black rectangle. For the polyprotein, the amino (N)- and carboxy (C)-terminal ends of the polyprotein are indicated. For the cleavage products, precursor proteins 1, 2, and 3 and their processed products are indicated; see the text for details.

Citation: Johnson K, Sarnow P. 1995. Viral RNA Synthesis, p 95-112. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch4
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Image of FIGURE 2
FIGURE 2

HF-dependent snapback model for the initiation of negative-strand viral RNA. Viral (VF, 3AB, 3B, 3D, and 3CD) and cellular (CF and HF) proteins and their putative actions in the replication process are shown. The direction of movement of the nascent RNA chain is indicated by two arrows. See the text for details. A hypothetical membrane is shown on the left. VF, viral factors; CF, cellular factors.

Citation: Johnson K, Sarnow P. 1995. Viral RNA Synthesis, p 95-112. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch4
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Image of FIGURE 3
FIGURE 3

3B (VPg)-primed model for the initiation of positive-strand RNA. Viral (VF, 3AB, 3B, 3D, and 3CD) and cellular (CF and HF) proteins and their putative actions in the replication process are shown. The direction of movement of the nascent RNA chain is indicated by two arrows. See the text for details. A hypothetical membrane is shown on the left. VF, viral factors; CF, cellular factors.

Citation: Johnson K, Sarnow P. 1995. Viral RNA Synthesis, p 95-112. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch4
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Image of FIGURE 4
FIGURE 4

-Initiation model for the initiation of positive-strand RNAs. Viral (3AB, 3D, and 3CD) and cellular (p36) proteins and their putative actions in the replication process are shown. The cloverleaf-type RNA structure at the 5′ end of the positive-strand RNA is indicated. 3B (open circle) is shown attached to the 5′ ends of the positive strands and positioned as primer in the RC. See the text for details. A hypothetical membrane is shown on the left. This figure is reprinted from Andino et al. ( ) by permission of Oxford University Press.

Citation: Johnson K, Sarnow P. 1995. Viral RNA Synthesis, p 95-112. In Rotbart H (ed), Human Enterovirus Infections. ASM Press, Washington, DC. doi: 10.1128/9781555818326.ch4
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References

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1. Ambros, V.,, and D. Baltimore. 1978. Protein is linked to the 5′ end of poliovirus RNA by a phosphodiester linkage to tyrosine. J. Biol. Chem. 60:52635266.
2. Andino, R.,, G. E. Rieckhof,, P. L. Achacosco,, and D. Baltimore. 1993. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO J. 12:35873598.
3. Andino, R.,, G. E. Rieckhof,, and D. Baltimore. 1990. A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA. Cell 63:369380.
4. Andino, R.,, G. E. Rieckhof,, D. Trono,, and D. Baltimore. 1990. Substitutions in the protease 3C gene of poliovirus can suppress a mutation in the 5′ noncoding region. J. Virol. 64:607612.
5. Andrews, N.,, and D. Baltimore. 1986. Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction. Proc. Natl. Acad. Sci. USA 83:221225.
6. Andrews, N. C.,, and D. Baltimore. 1986. Lack of evidence for VpG priming of poliovirus RNA synthesis in the HF-dependent in vitro replicase reaction. J. Virol. 58:212215.
7. Andrews, N. C.,, D. Levin,, and D. Baltimore. 1985. Poliovirus replicase stimulation by TUTase. J. Biol. Chem. 260:76287635.
8. Baltimore, D. 1969. The Replication of Picornaviruses. Marcel Dekker, Inc., New York.
9. Barton, D.,, and J. B. Flanegan. 1993. Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus. J. Virol. 67:822831.
10. Bernstein, H. D.,, R. Sarnow,, and D. Baltimore. 1986. Genetic complementation among poliovirus mutants derived from an infectious cDNA clone. J. Virol. 60:10401049.
11. Bernstein, H. D.,, N. Sonenberg,, and D. Baltimore. 1985. Poliovirus mutant that does not selectively inhibit host cell protein synthesis. Mol. Cell. Biol. 5:29132923.
12. Bienz, K.,, D. Egger,, and L. Pasamontes. 1987. Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160:220226.
13. Bienz, K.,, D. Egger,, and T. Pfister,. 1994. Characteristics of the poliovirus replication complex, p. 147157. In M. A. Brinton,, C. H. Calisher,, and R. Rueckert (ed.), Positive-Strand RNA Viruses. Springer-Verlag, Vienna.
14. Bienz, K.,, D. Egger,, T. Pfister,, and M. Troxler. 1992. Structural and functional characterization of the poliovirus replication complex. J. Virol. 66:27402747.
15. Bienz, K.,, D. Egger,, Y. Rasser,, and W. Bossart. 1980. Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology 100:390399.
16. Bienz, K.,, D. Egger,, Y. Rasser,, and W. Bossart. 1983. Intracellular distribution of poliovirus proteins and the induction of virus-specific cytoplasmic structures. Virology 131:3948.
17. Bienz, K.,, D. Egger,, M. Troxler,, and L. Pasamontes. 1990. Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region. J. Virol. 64:11561163.
18. Burns, C. C.,, O. C. Richards,, and E. Ehrenfeld. 1992. Temperature-sensitive poliovirus containing mutations in RNA polymerase. Virology 189:568582.
19. Butterworth, B. E.,, E. J. Shimshick,, and F. H. Yin. 1976. Association of the polioviral RNA polymerase complex with phospholipid membranes. J. Virol. 19:457466.
20. Caliguiri, L. A.,, and I. Tamm. 1970. Characterization of poliovirus-specific structures associated with cytoplasmic membranes. Virology 42:112122.
21. Cho, M. W.,, N. Teterina,, D. Egger,, K. Bienz,, and E. Ehrenfeld. 1994. Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells. Virology 202:129145.
22. Cole, C. N.,, D. Wimmer,, and D. Baltimore. 1971. Defective interfering particles of poliovirus. I. Isolation and physical properties. J. Virol. 7:478485.
23. Collis, P. S.,, J. B. O'Donnell,, D.J. Barton,, J. A. Rogers,, and J. B. Flanegan. 1992. Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells. J. Virol. 66:64806488.
24. Cooper, P. 1968. A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584596.
25. Cooper, P. D. 1964. An improved agar cell-suspension plaque assay for poliovirus: some factors affecting efficiency of plating. Virology 13:153157.
26. Cooper, P. D. 1965. Rescue of one pheno-type in mixed infections with heat-defective mutants of type 1 poliovirus. Virology 25:431438.
27. Cooper, P. D. 1977. Genetics of picornaviruses. Comp. Virol. 9:133207.
28. Crawford, N. M.,, and D. Baltimore. 1983. Genome-linked protein VPg of poliovirus is present as free VPg and VPg-pUpU in poliovirus-infected cells. Proc. Natl. Acad. Sci. USA 80:74527455.
29. Dales, S.,, H. J. Eggers,, I. Tamm,, and G. E. Palade. 1965. Electron microscopic study of the formation of poliovirus. Virology 26:379389.
30. Dasgupta, A.,, M. H. Baron,, and D. Baltimore. 1979. Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA. Proc. Natl. Acad. Sci. USA 76:26792683.
31. Dasgupta, A.,, P. Zabel,, and D. Baltimore. 1980. Dependence of the activity of the poliovirus replicase on a host cell protein. Cell 19:423429.
32. Datta, U.,, and A. Dasgupta. 1994. Expression and subcellular localization of poliovirus VPg-precursor protein 3AB in eukaryotic cells: evidence for glycosylation in vitro. J. Virol. 68:44684477.
33. Diamond, S. E.,, and K. Kirkegaard. 1994. Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis. J. Virol. 68:863876.
33a. Doedens, J.,, and K. Kirkegaard. Inhibition of protein secretion by poliovirus proteins 2B and 3A. EMBO J., in press.
34. Doedens, J.,, L. A. Maynell,, M. W. Klymkowski,, and K. Kirkegaard,. 1994. Secretory pathway function, but not cytoskeletal integrity, is required in poliovirus infection, p. 159172. In M. A. Brinton,, C. H. Calisher,, and R. Rueckert (ed.), Positive-Strand RNA Viruses. Springer-Verlag, Vienna.
35. Ehrenfeld, E.,, J. V Maizel,, and D. F. Summers. 1970. Soluble RNA polymerase complex from poliovirus-infected HeLa cells. Virology 40:840846.
36. Evans, D. M.,, G. Dunn,, P. D. Minor,, G. C. Schild,, A. J. Cann,, G. Stanway,, J. W. Almond,, K. Currey,, and J.V. Maizel. 1985. Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature (London) 314:548550.
37. Flanegan, J. B.,, and D. Baltimore. 1977. Poliovirus-specific primer-dependent RNA polymerase able to copy poly (A). Proc. Natl. Acad. Sci. USA 74:36773680.
38. Flanegan, J. B.,, R. F. Pettersson,, V. Ambros,, M. J. Hewlett,, and D. Baltimore. 1977. Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus. Proc. Natl. Acad. Sci. USA 74:961965.
39. Flanegan, J. B.,, and T. A. van Dyke. 1979. Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro. J. Virol. 32:155161.
40. Giachetti, C.,, S. S. Hwang,, and B. L. Semler. 1992. cis-Acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication. J. Virol. 66:60456057.
41. Giachetti, C.,, and B. L. Semler. 1991. Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis. J. Virol. 65:26472654.
42. Girard, M.,, D. Baltimore,, and J. E. Darnell. 1967. The poliovirus replication complex: sites for synthesis of poliovirus RNA. J. Mol. Biol. 24:5974.
43. Hambidge, S. J.,, and P. Sarnow. 1992. Translation enhancement of the poliovirus 5′ noncoding region mediated by virus-encoded polypeptide 2A. Proc. Natl. Acad. Sci. USA 89:1027210276.
44. Hanecak, R.,, B. L. Semler,, C. W. Anderson,, and E. Wimmer. 1982. Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage of glutamine-glycine pairs. Proc. Natl. Acad. Sci. USA 79:39733977.
45. Harmon, S. A.,, O. C. Richards,, D. F. Summers,, and E. Ehrenfeld. 1991. The 5′-terminal nucleotides of hepatitis A virus RNA, but not poliovirus RNA, are required for infectivity. J. Virol. 65:27572760.
46. Iizuka, N.,, S. Kuge,, and A. Nomoto. 1987. Complete nucleotide sequence of the genome of coxsackievirus Bl. Virology 156:6473.
47. Irurzun, A.,, L. Perez,, and L. Carrasco. 1992. Involvement of membrane traffic in the replication of poliovirus genomes: effect of brefeldin A. Virology 191:166175.
48. Jacobson, S. J.,, D. A. M. Konings,, and P. Sarnow. 1993. Biochemical and genetic evidence for a pseudoknot structure at the 3′ terminus of the poliovirus RNA genome and its role in viral RNA amplification. J. Virol. 67:29612971.
49. Jang, S. K.,, H. G. Krausslich,, M. J. H. Nicklin,, G. M. Duke,, A. C. Palmenberg,, and E. Wimmer. 1988. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J. Virol. 62:26362643.
50. Johnson, K. L.,, and P. Sarnow. 1991. Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus. J. Virol. 65:43414349.
51. Kaplan, G.,, and V. R. Racaniello. 1988. Construction and characterization of poliovirus subgenomic replicons. J. Virol. 62:16871696.
52. Kirkegaard, K. 1992. Genetic analysis of pi-cornaviruses. Curr. Opin. Genet. Dev. 2:6470.
53. Klump, W. M.,, I. Bergmann,, B. C. Müller,, D. Ameis,, and R. Kandolf. 1990. Complete nucleotide sequence of infectious coxsackievirus B3 cDNA: two initial 5′ uridine residues are regained during plus-strand synthesis. J. Virol. 64:15731583.
54. Kuge, S.,, I. Saito,, and A. Nomoto. 1986. Primary structure of poliovirus defective-interfering particle genomes and possible generation mechanisms of the particles. J. Mol. Biol. 192:473487.
55. Kuhn, R. J.,, H. Tada,, M. E Ypma-Wong,, J. J. Dunn,, B. L. Semler,, and E. Wimmer. 1988. Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants. Proc. Natl. Acad. Sci. USA 85:519523.
56. Kuhn, R. J.,, H. Tada,, M. F. Ypma-Wong,, B. L. Semler,, and E. Wimmer. 1988. Mutational analysis of the genome-linked protein VPg of poliovirus. J. Virol. 62:42074215.
57. Kuhn, R. J.,, and E. Wimmer,. 1987. The replication of piconaviruses, p. 1751. In D.J. Rowlands,, M. A. Mayo,, and B. W. J. Mahy (ed.), The Molecular Biology of Positive Strand RNA Viruses. Acad8emic Press, London.
58. Lama, J.,, A. V. Paul,, K. V. Harris,, and E. Wimmer. 1994. Properties of purified recombinant poliovirus 3AB as substrate for viral proteinases and as co-factor for RNA polymerase 3Dpol J. Biol. Chem. 269:6670.
59. Lawson, M. A.,, and B. L. Semler. 1990. Picornavirus protein processing: enzymes, substrates and genetic regulation. Curr. Top. Microbiol. Immunol. 161:4988.
60. Lee, Y. F.,, A. Nomoto,, B. M. Detjen,, and E. Wimmer. 1977. A protein covalently linked to poliovirus genome RNA. Proc. Natl. Acad. Sci. USA 74:5963.
61. Levintow, L.,, M. M. Thoren,, J. E. Darnell,, and J. L. Hooper. 1962. Effect of p-fluorophenylalanine and puromycin on the replication of poliovirus. Virology 16:220229.
62. Li, J.-P.,, and D. Baltimore. 1988. Isolation of poliovirus 2C mutants defective in viral RNA synthesis. J. Virol. 62:40164021.
63. Maynell, L. A.,, K. Kirkegaard,, and M. W. Kymkowsky. 1992. Inhibition of poliovirus RNA synthesis by brefeldin A. J. Virol. 66:19851994.
64. Mendelsohn, C. L.,, E. Wimmer,, and V. R. Racaniello. 1989. Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56:855865.
65. Molla, A.,, A. V. Paul,, and E. Wimmer. 1991. Cell-free, de novo synthesis of poliovirus. Science 254:16471651.
66. Mosser, A. G.,, L. A. Caliguiri,, and I. Tamm. 1972. Incorporation of lipid precursors into cytoplasmic membranes of poliovirus-infected HeLa cells. Virology 47:3947.
67. Nomoto, A.,, N. Kitamura,, F. Golini,, and E. Wimmer. 1977. The 5′-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proc. Natl. Acad. Sci. USA 74:53455349.
68. Novak, J. E.,, and K. Kirkegaard. 1991. Improved method for detecting poliovirus negative strands used to demonstrate specificity of positive-strand encapsidation and the ratio of positive to negative strands in infected cells. J. Virol. 65:33843387.
69. Novak, J. E.,, and K. Kirkegaard. 1994. Coupling between translation and replication in an RNA virus. Genes Dev. 8:17261737.
70. Oh, S. K.,, and P. Sarnow. 1993. Gene regulation: translational initiation by internal ribosome binding. Curr. Opin. Genet. Dev. 3:295300.
71. Orci, L.,, M. Tagaya,, M. Amherdt,, A. Perrelet,, J. G. Donaldson,, J. Lippincott-Schwartz,, R. D. Klausner,, and J. E. Rothman. 1991. Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae. Cell 64:11831195.
72. Palmenberg, A. C. 1987. Comparative organization and genome structure in picornaviruses. UCLA Symp. Mol. Cell. Biol. 54:2534.
73. Pelletier, J.,, and N. Sonenberg. 1988. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature (London) 334:320325.
74. Pettersson, R. P.,, J. B. Flanegan,, J. K. Rose,, and D. Baltimore. 1977. 5′-Terminal nucleotide sequence of poliovirus polyribosomal RNA and virion RNA are identical. Nature (London) 268:270272.
75. Pfister, T.,, L. Pasamontes,, M. Toxler,, D. Egger,, and K. Bienz. 1992. Immunocy-tochemical localization of capsid-related proteins in subcellular fractions of poliovirus-infected cells. Virology 188:676684.
76. Pincus, S. E.,, D. C. Diamond,, E. A. Emini,, and E. Wimmer. 1986. Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C. J. Virol. 57:638646.
77. Pincus, S. E.,, H. Rohl,, and E. Wimmer. 1987. Guanidine-dependent mutants of poliovirus: identification of three classes with different growth requirements. Virology 157:8388.
78. Plotch, S. J.,, O. Palant,, and Y. Gluzman. 1989. Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli. J. Virol. 63:216225.
79. Putnak, J. R.,, and B. A. Phillips. 1981. Picornaviral structure and assembly. Microbiology 45:287315.
80. Racaniello, V. R.,, and D. Baltimore. 1981. Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916919.
81. Racaniello, V. R.,, and C. Meriam. 1986. Poliovirus temperature-sensitive mutant containing a single nucleotide deletion in the 5′-noncoding region of the viral RNA. Virology 155:498507.
82. Richards, O. C.,, and E. Ehrenfeld. 1990. Poliovirus RNA replication. Curr. Top. Microbiol. Immunol. 161:89120.
83. Rivera, V. M.,, J. D. Welsh,, and J. V. Maizel. 1988. Comparative sequence analysis of the 5′ noncoding region of the enteroviruses and rhinoviruses. Virology 165:4250.
84. Rothberg, P. G.,, T. J. R Harris,, A. Nomoto,, and E. Wimmer. 1978. The genome-linked protein of picornaviruses. V. O4-(5′uridylyl)-ty-rosine is the bond between the genome-linked protein and the RNA of poliovirus. Proc. Natl. Acad. Sci. USA 75:48684872.
85. Rueckert, R. R., 1990. Piconaviridae and their replication, p. 507548. In B. N. Fields et al. (ed.), Virology, 2nd ed., vol. 1. Raven Press, New York.
86. Sarnow, P. 1989. Role of 3′-end sequences in infectivity of poliovirus transcripts made in vitro. J. Virol. 63:467470.
87. Sarnow, P.,, H. D. Bernstein,, and D. Baltimore. 1986. A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome. Proc. Natl. Acad. Sci. USA 83:571575.
88. Sarnow, P.,, S. J. Jacobson,, and L. Najita. 1990. Poliovirus genetics. Curr. Top. Microbiol. Immunol. 161:155188.
89. Sedivy, J. M.,, J. P. Capone,, U. L. RajBhandary,, and P. A. Sharp. 1987. An inducible mammalian amber suppressor: propagation of a poliovirus mutant. Cell 50:379389.
90. Semler, B. L.,, C. W. Anderson,, R. Hanecak,, L. Dorner,, and E. Wimmer. 1982. A membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthesis heptapeptide. Cell 28:405412.
91. Skinner, M. A.,, V. R. Racaniello,, G. Dunn,, J. R. Cooper,, P. D. Minor,, and J. W. Almond. 1989. New model for the secondary structure of the 5′ non-coding RNA of poliovirus is supported by biochemical and genetic data that also shows that RNA secondary structure is important in neurovirulence. J. Mol. Biol. 207:379392.
92. Takeda, N.,, R. J. Kuhn,, C. F. Yang,, T. Takegami,, and E. Wimmer. 1986. Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells. J. Virol. 60:4353.
93. Takegami, T.,, R. J. Kuhn,, C. W. Anderson,, and E. Wimmer. 1983. Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus. Proc. Natl. Acad. Sci. USA 80:74477451.
94. Takegami, T.,, B. L. Semler,, C. W. Anderson,, and E. Wimmer. 1983. Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides. Virology 128:3347.
95. Tobin, G. J.,, D. C. Young,, and J. B. Flanegan. 1989. Self-catalyzed linkage of poliovirus terminal protein VPg to poliovirus RNA. Cell 59:511519.
96. Toyoda, H.,, M. Kohara,, Y. Kataoka,, T. Suganuma,, T. Omata,, N. Imura,, and A. Nomoto. 1984. Complete nucleotide sequences of all three poliovirus serotype genomes: implication for genetic relationship, gene function and antigenic determinants. J. Mol. Biol. 174:561585.
97. Toyoda, H.,, M. J. H. Nicklin,, M. G. Murray,, C. W. Anderson,, J. J. Dunn,, F. W. Studier,, and E. Wimmer. 1986. A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell 45:761770.
98. Troxler, M.,, D. Egger,, T. Pfister,, and K. Bienz. 1992. Intracellular localization of poliovirus RNA by in situ hybridization at the ultrastructural level using single-stranded riboprobes. Virology 191:687697.
99. van der Werf, S.,, J. Bradley,, E. Wimmer,, F. W. Studier,, and J. J. Dunn. 1986. Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:23302334.
100. van Dyke, T. A.,, and J. B. Flanegan. 1980. Identification of poliovirus polypeptide p63 as a soluble RNA-dependent RNA polymerase. J. Virol. 35:732740.
101. Ward, C. D.,, and J. B. Flanegan. 1992. Determination of the poliovirus RNA polymerase error frequency at eight sites in the viral genome. J. Virol. 66:37843793.
102. Ward, C. D.,, M. A. Stokes,, and J. B. Flanegan. 1988. Direct measurement of the poliovirus RNA polymerase error frequency in vitro. J. Virol. 62:558562.
103. White, J. M.,, and D. R. Littman. 1989. Viral receptors of the immunoglobulin super-family. Cell 56:725728.
104. Wimmer, E.,, C. U. T. Hellen,, and X. Cao. 1993. Genetics of poliovirus. Annu. Rev. Genet. 27:353436.
105. Yogo, Y.,, and E. Wimmer. 1975. Sequence studies of poliovirus RNA. III. Polyuridylic acid and polyadenylic acid as components of the purified poliovirus replicarive intermediate. J. Mol. Biol. 92:467477.
106. Young, D. C.,, D. M. Tuschall,, and J. B. Flanegan. 1985. Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro. J. Virol. 54:256264.
107. Ypma-Wong, M. F.,, P. G. Dewalt,, V. H. Johnson,, J. G. Lamb,, and B. L. Semler. 1988. Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursor. Virology 166:265270.

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