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Chapter 2 : R Plasmids and Antibiotic Resistances

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Abstract:

It has been estimated that at least 356 extra-chromosomal elements can introduce genetic information into , an amount of exogenously contributed DNA, in the form of plasmids, equal to or greater than the DNA content of the chromosome. The mole fraction G+C values would provide a clue to their origin(s) since the chromosome has a G+C ratio of 0.50, in comparison with a G+C ratio of 0.38 for the chromosome. The author and his coworkers found mole fraction G+C values of 0.39 and 0.40 for plasmids RSF007 and RSF0885, respectively, values that are closer to those for than . To visualize in more detail the exact nature of the TnA sequence in both plasmids, they heteroduplexed each plasmid with R648, a replicon containing the entire ampicillin transposon with the added feature of one EcoRI cleavage site that served to orient TnA in heteroduplex analyses. The incidence of R-plasmid-mediated drug resistance among clinical isolates is most evident in highly selective environments such as intensive care units and burn wards, where the daily administration of high-potency antibiotics is often a necessity. The identities of two conjugative R plasmids isolated from bacterial strains from two gram-negative genera originating in a hospital burn unit were proved, by a variety of molecular techniques. Drug-resistant strains cause otitis media meningitis among children in day-care centers, and in hospitals, clinicians grapple with multidrug-resistant and species.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2

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Chromosomal DNA
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Conjugative Plasmids
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Figures

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Figure 1

Ethidium bromide-CsCl gradient of a cleared lysate of G32(RSF007) grown in minimal medium in the presence of [H]thymidine.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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Figure 2

Electron micrograph of ampicillin-resistant plasmid RSF007. CCC, covalently closed circular form; OC, open circular form. Final magnification, x 13,874.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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Figure 3

Heteroduplexes of RSF007 and RSF0885 plasmid DNA with RI-cut R648 plasmid DNA. (A) RI endonuclease-cut single-stranded molecule of R648. The Tn region is marked by the inverted repeats that reanneal in a single-stranded molecule, forming a double-stranded region (arrow). (B) Heteroduplex of RSF007 and RI-cut R648. (C) Single- and double-stranded molecules of RSF0885. Note the absence of an inverted repeat nucleotide base sequence in the single-stranded molecule. (D) Heteroduplex of RSF0885 and RI-cut R648. SS, single-stranded DNA; DS, double-stranded DNA; IR, inverted repeat nucleotide base sequence.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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Figure 4

(A). Heteroduplex of RSF007 and pUB701 plasmid DNA. (B) Diagram of RSF007/pUB701 heteroduplex. Thick line, double-stranded DNA; thin line, single-stranded DNA; Ap, ampicillin resistance transposon Tn; Tc, tetracycline resistance transposon (Tn); IR, inverted repeat sequence.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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Figure 5

Agarose gel electrophoresis of ethanol-precipitated DNA from cleared lysates of penicillin-susceptible (Pen) and penicillin-resistant (Pen) strains. Lanes: A, strain KH45 (Pen) containing the 24.5 x 10-Da cryptic plasmid; B, strain F62 (Pen) containing the 2.6 x 10-Da cryptic plasmid; C, strain CDC66 (Pen), Far East isolate; D, strain CDC67 (Pen), Far East isolate; E, spontaneous Pen derivative of strain CDC01; F, strain CDC01 (Pen), from a case contact of a patient infected in the Far East; G, strain IPL (Pen) from London, England; H, standard plasmid DNAs ranging in size from 62 x 10 (uppermost band) to 1.9 x 10 (lowest band) Da. OC refers to the open circular form of the 2.6 x 10-Da cryptic plasmid. Numbers indicate molecular masses (in megadaltons). CHR indicates chromosomal DNA.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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Figure 6

Agarose gel electrophoresis of ethanol-precipitated DNA from cleared lysates of tobramycin-resistant (TOB) and tobramycin-susceptible strains. Plasmid pIE098 is a 64-MDa conjugative R plasmid specifying resistances to tobramycin, kanamycin, neomycin, streptomycin, and ampicillin. Lanes: A, 185(R); B, TOB clinical isolate; C and D, 185(pIE098), derived from mating with the TOB strain; E, TOB clinical isolate; F and G, 185(pIE098), derived from mating with the TOB isolate; H, purified standard plasmids for molecular weight determinations. Numbers indicate megadaltons. CHR. DNA, chromosomal DNA.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
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References

/content/book/10.1128/9781555818340.chap2
1. Albritton, W. L.,, J. L. Brunton,, L. Slaney,, and I. MacLean. 1982. Plasmid-mediated sulfonamide resistance in Haemophilus ducreyi. Antimicrob. Agents Chemother. 21:159165.
2. Albritton, W. L.,, I. W. MacLean,, L. A. Slaney,, A. R. Ronald,, and H. Deneer. 1984. Plasmid-mediated tetracycline resistance in Haemophilus ducreyi. Antimicrob. Agents Chemother. 25: 187190.
3. Ashford, W. A.,, R. G. Golash,, and V. G. Hemming. 1976. Penicillinase-producing Neisseria gonorrhoeae. Lancet 11:657658.
4. Barth, P. T.,, and N. J. Grinter. 1974. Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. J. Bacteriol. 120:618630.
5. Brunton, J.,, D. Clare,, and M. A. Meier. 1986. Molecular epidemiology of antibiotic resistance plasmids of Haemophilus species and Neisseria gonorrhoeae. Rev. Infect. Dis. 8:713724.
6. Chen, S.-T.,, and R. C. Clowes. 1987. Nucleotide sequence comparisons of plasmids pHDI31, pJBl, pFA3, and pFA7 and β-lactamase expression in Escherichia coli, Haemophilus influenzae, and Neisseria gonorrhoeae. J. Bacteriol. 169:31243130.
7. Crosa, J. H.,, J. Brenner,, and S. Falkow. 1973. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115: 904911.
8. De Graaff, J.,, L. P. Elwell,, and S. Falkow. 1976. The molecular nature of two beta-lactamase specifying plasmids isolated from Haemophilus influenzae type b. J. Bacteriol. 126:439446.
9. Dickgiesser, N. 1984. A molecular characterization of ft. influenzae plasmid pVe445: a comparison with N. gonorrhoeae plasmids pNGlO and pNG18. Plasmid 11:99101.
10. Dickgiesser, N.,, P. M. Bennett,, and M. N. Richmond. 1982. Penicillinase-producing Neisseria gonorrhoeae and molecular comparison of 5.3- and 7.4-kilobase β-lactamase plasmids. J. Bacteriol. 151:11711175.
11. Dillon, J. R.,, M. Pauze,, and K. H. Yeung. 1983. Spread of penicillinase-producing and transfer plasmids from the gonococcus to Neisseria meningitidis. Lancet 1:779781.
12. Eisenstein, B. I.,, T. Sox,, G. Biswas,, E. Blackman,, and P. F. Sparling. 1977. Conjugal transfer of the gonoccocal penicillinase plasmid. Science 195:9981000.
13. Elwell, L. P.,, J. de Graaff,, D. Siebert,, and S. Falkow. 1975. Plasmid-linked ampicillin resistance in Haemophilus influenzae type b. Infect. Immun. 12:404410.
14. Elwell, L. P.,, and S. Falkow,. 1977. Plasmids of the genus Neisseria, p. 134154. In R. Roberts (ed.), The Gonococcus. John Wiley & Sons, Inc., New York.
15. Elwell, L. P.,, and S. Falkow. 1977. Genetic loose change. Sciences 17:811.
16. Elwell, L. P.,, and S. Falkow,. 1986. The characterization of R plasmids and the detection of plasmid-specified genes, p. 683721. In V. Lorian (ed.), Antibiotics in Laboratory Medicine, 2nd ed. The Williams & Wilkins Co., Baltimore.
17. Elwell, L. P.,, J. Inamine,, and B. Minshew. 1978. Common plasmid specifying tobramycin resistance found in two enteric bacteria isolated from burn patients. Antimicrob. Agents Chemother. 13: 312317.
18. Elwell, L. P.,, M. Roberts,, and S. Falkow,. 1978. Common β-lactamase specifying R plasmid isolated from the genera Haemophilus and Neisseria, p. 255256. In D. Schlessinger (ed.), Microbiology—1978. American Society for Microbiology, Washington, D.C.
19. Elwell, L. P.,, M. Roberts,, L. Mayer,, and S. Falkow. 1977. Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae. Antimicrob. Agents Chemother. 11:528533.
20. Elwell, L. P.,, J. R. Saunders,, M. H. Richmond,, and S. Falkow. 1977. Relationships among some R plasmids found in Haemophilus influenzae. J. Bacteriol. 131:356362.
21. Engelkirk, P. G.,, and D. E. Schoenhard. 1972. Physical evidence of a plasmid in Neisseria gonorrhoeae. J. Infect. Dis. 127:197200.
22. Faclnelli, B.,, and P. E. Varaldo. 1987. Plasmid-mediated sulfonamide resistance in Neisseria meningitidis. Antimicrob. Agents Chemother. 31:16421643.
23. Falkow, S.,, L. P. Elwell,, J. de Graaff,, F. Heffron,, and L. Mayer,. 1976. A possible model for the development of plasmid-mediated penicillin-resistance in the gonococcus, p. 20133. In R. D. Catterall, and C. S. Nicol (ed.), Sexually Transmitted Diseases. Academic Press Publishers, London.
24. Falkow, S.,, L. P. Elwell,, M. Roberts,, F. Heffron,, and R. Gill,. 1977. The transposition of ampicillin resistance: nature of ampicillin resistant H. influenzae and N. gonorrhoeae, p. 115125. In J. Drews, and G. Hogenauer (ed.), R-Factors: Their Properties and Possible Control. Springer-Verlag, New York.
25. Flett, F.,, G. O. Humphreys,, and J. R. Saunders. 1981. Intraspecific and intergeneric mobilization of non-conjugative resistance plasmids by a 24.5 Mdal conjugative plasmid of Neisseria gonorrhoeae. J. Gen. Microbiol. 125:123129.
26. Genco, C. A.,, J. S. Knapp,, and V. L. Clark. 1984. Conjugation of plasmids of Neisseria gonorrhoeae to other Neisseria species: potential reservoirs for the ^-lactamase plasmid. J. Infect. Dis. 150:397401.
27. Handsfield, H. H.,, E. G. Sandstrom,, J. S. Knapp,, P. Perine,, W. L. Whittington,, D. E. Sayers,, and K. K. Holmes. 1982. Epidemiology of penicillinase-producing Neisseria gonorrhoeae infections: analysis by auxotyping and serotyping. N. Engl. J. Med. 306:950954.
28. Heffron, F.,, C. Rubens,, and S. Falkow. 1975. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. USA 72:36233627.
29. Heffron, F.,, R. Sublett,, R. W. Hedges,, A. Jacob,, and S. Falkow. 1975. Origin of the TEM beta-lactamase gene found on plasmids. J. Bacteriol. 122:250256.
30. Hinshelwood, C. 1946. The Chemical Kinetics of the Bacterial Cell. Oxford University Press (Clarendon), London.
31. Ikeda, F.,, A. Tsuji,, Y. Kaneko,, M. Nishida,, and S. Goto. 1986. Conjugal transfer of beta-lactamase-producing plasmids of Neisseria gonorrhoeae to Neisseria meningitidis. Microbiol. Immunol. 30: 737742.
32. Ison, C. A.,, C. M. Beillinger,, and J. Walker. 1986. Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica. J. Clin. Pathol. 39:11191123.
33. Jaffe, H. W.,, J. W. Biddle,, S. R. Johnson,, and J. J. Wiesner. 1981. Infections due to penicillinase-producing Neisseria gonorrhoeae in the United States: 1976-1980. J. Infect. Dis. 144:191197.
34. Jahn, G.,, R. Laufs,, P.-M. Kaulfers,, and H. Kolenda. 1979. Molecular nature of two Haemophilus influenzae R factors containing resistances and the multiple integration of drug resistance transposons. J. Bacteriol. 138:584597.
35. Kahn, W.,, S. Ross,, W. Rodriguez,, G. Controri,, and A. K. Saz. 1974. Haemophilus influenzae type B resistant to ampicillin. A report of two cases. JAMA 229:298301.
36. Kaulfers, P.-M.,, R. Laufs,, and G. Jahn. 1978. Molecular properties of transmissible R factors of Haemophilus influenzae determining tetracycline resistance. J. Gen. Microbiol. 105:243252.
37. Kilian, M. 1976. A taxonomic study of the genus Haemophilus with the proposal of a new species. J. Gen. Microbiol. 93:962.
38. Kirven, L. A.,, and C. Thornsberry. 1977. Transfer of beta-lactamase genes of Neisseria gonorrhoeae by conjugation. Antimicrob. Agents Chemother. 11:10041006.
39. Korch, C.,, P. Hagblom,, H. Ohman,, M. Goransson,, and S. Normark. 1985. Cryptic plasmid of Neisseria gonorrhoeae: complete nucleotide sequence and genetic organization. J. Bacteriol. 163: 430438.
40. Laufs, R.,, F.-C. Riess,, G. Jahn,, R. Fock,, and P.-M. Kaulfers. 1981. Origin of Haemophilus influenzae R factors. J. Bacteriol. 147:563568.
41. Maness, M. J.,, and P. F. Sparling. 1973 Multiple antibiotic resistance due to a single mutation in Neisseria gonorrhoeae. J. Infect. Dis. 128:321326.
42. Marshall, B.,, M. C. Roberts,, A. Smith,, and S. B. Levy. 1984. Homogeneity of transferable tetracycline-resistance determinants in Haemophilus species. J. Infect. Dis. 149:10281029.
43. Mayer, L. W.,, K. K. Holmes,, and S. Falkow. 1974. Characterization of plasmid deoxyribonucleic and from Neisseria gonorrhoeae. Infect. Immun. 10:712717.
44. McNicol, P. J.,, W. L. Albritton,, and A. R. Ronald. 1986. Transfer of plasmid-mediated ampicillin resistance from Haemophilus to Neisseria gonorrhoeae requires an intervening organism. Sex. Transm. Dis. 13:145150.
45. Meyers, J.,, D. Sanchez,, L. P. El well,, and S. Falkow. 1976. A simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J. Bacteriol. 127:15291537.
46. Minshew, B. H.,, R. K. Holmes,, J. P. Sanford,, and C. R. Baxter. 1974. Transferable resistance to tobramycin in Klebsiella pneumoniae and Enterobacter cloacae associated with enzymatic acetylation of tobramycin. Antimicrob. Agents Chemother. 6:492497.
47. Nelson, J. D. 1974. Should ampicillin be abandoned for treatment of Haemophilus influenzae disease? JAMA 229:322324.
48. Olsen, R. H.,, and P. Shipley. 1973. Host range and properties of the Pseudomonas aeruginosa R-factor R1822. J. Bacteriol. 113:772779.
49. Percival, A.,, J. E. Corkill,, P. O. Arya,, S. Rowlands,, C. D. Alergant,, and E. H. Anrels. 1976. Penicillin-producing gonococci in Liverpool. Lancet ii:13791382.
50. Phillips, I. 1976. Beta-lactamase-producing penicillin-resistant gonococcus. Lancet ii:656657.
51. Piffaretti, J. C.,, J. Frey,, and A. Arini. 1986. A new conjugative system for transferring plasmids into Neisseria gonorrhoeae, abstr. H-98, p. 143. Abstr. 86th Annu. Meet. Am. Soc. Microbiol. 1986. American Society for Microbiology, Washington, D.C.
52. Piffaretti, J. C.,, and L. Soldati. 1990. TnA transposons can be introduced and maintained in Neisseria gonorrhoeae. Res. Microbiol. 141:519528.
53. Pintado, C.,, C. Salvador,, R. Rotger,, and C. Nombela. 1985. Multiresistant plasmid from commensal Neisseria species. Antimicrob. Agents Chemother. 27:120124.
54. Reanney, D. 1976. Extrachromosomal elements as possible agents of adoption and development. Bacteriol. Rev\ 40:552590.
55. Roberts, M.,, L. P. Elwell,, and S. Falkow. 1977. Molecular characterization of two R-plasmids isolated from Neisseria gonorrhoeae. J. Bacteriol. 131:557563.
56. Roberts, M.,, L. P. Elwell,, and S. Falkow,. 1978. Introduction to the mechanisms of genetic exchange in the gonococcus: plasmids and conjugation in Neisseria gonorrhoeae, p. 3843. In G. Brooks,, E. Brooks,, E. Gotschlich,, K. Holmes,, W. Sawyer,, and F. Young (ed.), Immunobiology of Neisseria gonorrhoeae. American Society for Microbiology, Washington, D.C.
57. Roberts, M.,, and S. Falkow. 1977. Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature (London) 266:630631.
58. Roberts, M.,, P. Piot,, and S. Falkow. 1979. The ecology of gonococcal plasmids. J. Gen. Microbiol. 114:491494.
59. Roberts, M. C. 1989. Plasmids of Neisseria gonorrhoeae and other Neisseria species. Clin. Microbiol. Rev. 2:S18S23.
60. Roberts, M. C.,, C. D. Swenson,, L. M. Owens,, and A. L. Smith. 1980. Characterization of chloramphenicol-resistant Haemophilus influenzae. Antimicrob. Agents Chemother. 18:610615.
61. Roberts, M. C.,, and J. S. Knapp. 1988. Transfer of β-lactamase plasmids from Neisseria gonorrhoeae to Neisseria meningitidis and commensal Neisseria species by the 25.2-megadalton plasmid. Antimicrob. Agents Chemother. 32:14301432.
62. Rotger, R.,, E. Garcia-Valdes,, and E. P. Trallero. 1986. Characterization of a β-lactamase-specifying plasmid isolated from Eikenella corrodens and its relationship to a commensal Neisseria plasmid. Antimicrob. Agents Chemother. 30:508509.
63. Rotger, R.,, F. Rubio,, and C. Nombela. 1986. A multiresistance plasmid isolated from commensal Neisseria species is closely related to the enterobacterial plasmid RSF1010. J. Gen. Microbiol. 132:24912496.
64. Saunders, J.,, L. P. Elwell,, S. Falkow,, R. Sykes,, and M. Richmond. 1978. β-Lactamase and R-plasmids of Haemophilus influenzae. Scand. J. Infect. Dis. Suppl. 13:1622.
65. Scheifele, D. W.,, and S. J. Fussell. 1981. Frequency of ampicillin-resistant Haemophilus parainfluenzae in children. J. Infect. Dis. 143:495498.
66. Scheifele, D. W.,, S. J. Fussell,, and M. C. Roberts. 1982. Characterization of ampicillin-resistant Haemophilus parainfluenzae. Antimicrob. Agents Chemother. 21:734739.
67. Schaberg, D. R.,, A. K. Highsmith,, and I. K. Wachsmuth. 1977. Resistance plasmid transfer by Serratia marcescens in urine. Antimicrob. Agents Chemother. 11:449450.
68. Smith, A. L. 1983. Antibiotic resistance in Haemophilus influenzae. Pediatr. Infect. Dis. 2: 352355.
69. So, M.,, R. Gill,, and S. Falkow. 1975. The generation of a ColEl-Apr cloning vehicle which allows detection of inserted DNA. Mol. Gen. Genet. 142:239249.
70. Sox, T. E.,, W. Mohammed,, and P. F. Sparling. 1979. Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function. J. Bacteriol. 138:510518.
71. Sparling, P. F.,, T. Sox,, W. Mohammed,, and L. Guymon,. 1978. Antibiotic resistance in the gonococcus: diverse mechanisms of coping with a hostile environment, p. 4452. In G. Brooks,, E. Brooks,, E. Gotschlich,, K. Holmes,, W. Sawyer,, and F. Young (ed.), Immunobiology of Neisseria gonorrhoeae. American Society for Microbiology, Washington, D.C.
72. Stiffler, P. W.,, S. A. Lerner,, M. Bohnhoff,, and J. A. Morello. 1975. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae. J. Bacteriol. 122:12931300.
73. Stuy, J. H. 1980. Chromosomally integrated conjugative plasmids are common in antibiotic-resistant Haemophilus influenzae. J. Bacteriol. 142:925930.
74. Thome, G. M.,, and W. E. Farrar. 1975. Transfer of ampicillin resistance between strains of Haemophilus influenzae type B. J. Infect. Dis. 132:276281.
75. van Embden, J. D. A.,, M. Dessens-Kroon,, and B. van Klingeren. 1985. A new β-lactamase plasmid in Neisseria gonorrhoeae. J. Antimicrob. Chemother. 15:247258.
76. van Klingeren, B.,, J. van Embden,, and M. Dessens-Kroon. 1977. Plasmid-mediated chloramphenicol resistance in Haemophilus influenzae. Antimicrob. Agents Chemother. 11:383387.
77. Verschueren, H.,, M. Dekegel,, D. Dekegel,, C. Gilquin,, and S. De Mayer. 1982. Plasmids in Neisseria meningitidis. Lancet 1:851852.
78. Yeung, K.-H.,, J. R. Dillon,, M. Pauze,, and E. Wallace. 1986. A novel 4.9-kilobase plasmid associated with an outbreak of penicillinase-producing Neisseria gonorrhoeae. J. Infect. Dis. 153:11621165.

Tables

Generic image for table
Table 1

Hybridization between H-labeled RSF0885 and RSF1030 plasmid DNA and whole-cell DNA

The degree of DNA-DNA duplex formation was assayed by the S1 endonuclease method ( ). The actual extent of binding of H-labeled RSF0885 and RSF1030 plasmid DNA with whole-cell (RSF0885) and (RSF1030) DNA was approximately 87%. All other reactions were normalized to these values taken as 100%. Each value shown is the average of three separate reactions.

NT, not tested.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
Generic image for table
Table 2

Properties of selected, naturally occurring, large-molecular-weight R plasmids

Ap, ampicillin; Tc, tetracycline; CAM, chloramphenicol acetyltransferase.

DK, Denmark; NL, Netherlands; FRG, West Germany; UK, United Kingdom; USA, United States.

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2
Generic image for table
Table 3

Molecular characteristics of small β-lactamase-specifying plasmids found in and species

Citation: Elwell L. 1994. R Plasmids and Antibiotic Resistances, p 17-41. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch2

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