1887

Chapter 25 : Role of Flagella in Campylobacter Pathogenesis

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $15.00

Preview this chapter:
Zoom in
Zoomout

Role of Flagella in Campylobacter Pathogenesis, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818340/9781555810825_Chap25-1.gif /docserver/preview/fulltext/10.1128/9781555818340/9781555810825_Chap25-2.gif

Abstract:

The thermophilic Campylobacters and together constitute one of the major causes of bacterial diarrhea worldwide. Variations in growth temperature, pH, and inorganic ion contents led to increases in expression of up to 2.6-fold over the basal level of expression in standard medium, suggesting that Campylobacters can modulate the compositions of their flagellar filaments and their motilities in direct response to environmental signals. Kanamycin-resistant, motile revertants were never obtained in the presence of DNase, indicating that these recombinational events were due to autolysis of cells and natural transformation mediated, intergenomic recombinational events. The lack of a suitable nonprimate disease model has precluded examination of nonflagellated isogenic mutants in a pathogenesis model, but several groups have examined the role of flagella in the invasion and adherence of eukaryotic cells in vitro. Flagellin genes were among the first Campylobacter genes to be cloned and mutated by site-specific mutagenesis protocols. The subsequent manipulation of these genes not only has led to greater understanding about the structure of an unusual flagellar filament but also has increased understanding of other aspects of the biology of these organisms. An understanding of the mechanisms by which Campylobacters regulate expression of flagellin genes and genes encoding alternate posttranslational modifications on the flagellins will contribute further to understanding of the molecular pathogenesis of these important pathogens.

Citation: Guerry P. 1994. Role of Flagella in Campylobacter Pathogenesis, p 383-389. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch25

Key Concept Ranking

Gene Expression and Regulation
0.5091668
Sodium Dodecyl Sulfate
0.45593017
Pour Plate Method
0.40378174
0.5091668
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555818340.chap25
1. Aim, R. A.,, P. Guerry,, M. E. Power,, H. Lior,, and T. J. Trust. 1991. Analysis of the role of flagella in the heat-labile Lior serotyping scheme of thermophilic Campylobacters by mutant allele exchange. J. Clin. Microbiol. 29:24382445.
2. Aim, R. A.,, P. Guerry,, M. E. Power,, and T. J. Trust. 1992. Variation in antigenicity and molecular weight of Campylobacter coli VC167 flagellin in different genetic backgrounds. J. Bacteriol. 174: 42304238.
3. Aim, R. A.,, P. Guerry,, and T. J. Trust. 1993. Distribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni. J. Bacteriol. 175:30513057.
4. Aim, R. A.,, P. Guerry,, and T. J. Trust. 1993. The Campylobacter sigma54 flagellin promoter is subject to environmental regulation. J. Bacteriol. 175:44484455.
5. Aim, R. A.,, P. Guerry,, and T. J. Trust. 1993. Significance of duplicated flagellin genes in Campylobacter. J. Mol. Biol. 230:359363.
6. Ames, G. F.-L.,, and K. Nikaido. 1985. Nitrogen regulation in Salmonella typhimurium. Identification of an ntrC protein binding site and definition of the concensus. EMBO J. 4:539547.
7. Black, R. E.,, M. M. Levine,, M. L. Clements,, T. P. Hughes,, and M. J. Blaser. 1988. Experimental Campylobacter jejuni infections in humans. J. Infect. Dis. 157:472479.
8. Butzler, J. P.,, and M. B. Skirrow. 1979. Campylobacter enteritis. Clin. Gastroenterol. 8:737765.
9. Caldwell, M. B.,, P. Guerry,, E. C. Lee,, and J. P. Burans. 1985. Reversible expression of flagella in Campylobacter jejuni. Infect. Immun. 50:941943.
10. Ely, B.,, and L. Shapiro,. 1984. Regulation of cell differentiation in Caulobacter crescentus. Microbial development, p. 126. In R. Losick, and L. Shapiro (ed.), Microbial Development. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y..
11. Fischer, S. H.,, and I. Nachamkin. 1991. Common and variable domains of the flagellin gene, flaA, in Campylobacter jejuni. Mol. Microbiol. 5:11511158.
12. Grant, C. C. R.,, M. E. Konkel,, W. Cieplak, Jr.,, and L. S. Tompkins. 1993. Role of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell cultures. Infect. Immun. 61:17641771.
13. Guerry, P.,, R. A. Aim,, M. E. Power,, S. M. Logan,, and T. J. Trust. 1991. The role of two flagellin genes in Campylobacter motility. J. Bacteriol. 173:47574764.
14. Guerry, P.,, R. A. Aim,, M. E. Power,, and T. J. Trust,. 1992. Molecular and structural analysis of Campylobacter flagellin, p. 267281. In I. Nachamkin,, M. J. Blaser,, and L. S. Tompkins (ed.), Campylobacter jejuni: Current Status and Future Trends. American Society for Microbiology, Washington, D.C..
15. Guerry, P.,, S. M. Logan,, S. Thornton,, and T. J. Trust. 1990. Genomic organization and expression of Campylobacter flagellin genes. J. Bacteriol. 172:18531860.
16. Harris, L. A.,, S. M. Logan,, P. Guerry,, and T. J. Trust. 1987. Antigenic variation of Campylobacter flagella. J. Bacteriol. 169:50665071.
17. Helmann, J. D.,, and M. J. Chamberlain. 1987. DNA sequence analysis suggests that expression of flagellar and chemotaxis genes in Escherichia coli and Salmonella typhimurium is controlled by an alternative sigma factor. Proc. Natl. Acad. Sci. USA 84:64226424.
18. Ishimoto, K.,, and S. Lory. 1989. Formation of pilin in Pseudomonas aeruginosa requires the RpoN subunit of RNA polymerase. Proc. Natl. Acad. Sci. USA 86:19541957.
19. Khawaja, R.,, K. Neote,, H. L. Bingham,, J. L. Penner,, and V. L. Chan. 1992. Cloning and sequence analysis of the flagellin gene of Campylobacter jejuni TGH9011. Curr. Microbiol. 24:213221.
20. Konkel, J. E.,, and L. A. Joens. 1989. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect. Immun. 57:29842990.
21. Kostrzynska, M.,, J. D. Betts,, J. W. Austin,, and T. J. Trust. 1991. Identification, characterization, and spatial location of two flagellin species in Helicobacter pylori flagella. J. Bacteriol. 173: 937946.
22. Lee, A.,, J. L. O'Rourke,, P. J. Barrington,, and T. J. Trust. 1986. Mucus colonization by Campylobacter jejuni: a mouse cecal model. Infect. Immun. 51:536546.
23. Logan, S. M.,, P. Guerry,, D. M. Rollins,, D. H. Burr,, and T. J. Trust. 1989. In vivo antigenic variation of Campylobacter flagellin. Infect. Immun. 57:25832585.
24. Logan, S. M.,, L. A. Harris,, and T. J. Trust. 1987. Isolation and characterization of Campylobacter flagellins. J. Bacteriol. 169:50725077.
25. Logan, S. M.,, and T. J. Trust. 1986. Location of epitopes on Campylobacter jejuni flagellin. J. Bacteriol. 168:739745.
26. Logan, S. M.,, T. J. Trust,, and P. Guerry. 1989. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J. Bacteriol. 171:30313038.
27. Martin, P. M.,, J. Mathiot,, J. Ipero,, M. Kirimat,, A. J. Georges,, and M.-C. Georges-Courbot. 1989. Immune response to Campylobacter jejuni and Campylobacter coli in a cohort of children from birth to 2 years of age. Infect. Immun. 57:25422546.
28. McSweegan, E.,, and R. I. Walker. 1986. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect. Immun. 53:141148.
29. Meyer, T. F.,, E. Billyard,, R. Haas,, S. Storzbach,, and M. So. 1984. Pilus genes of Neisseria gonorrhoeae: chromosomal organization and DNA sequence. Proc. Natl. Acad. Sci. USA 81:61106114.
30. Minnich, S. A.,, and A. Newton. 1987. Promoter mapping and cell cycle regulation of flagellin gene transcription in Caulobacter crescentus. Proc. Natl. Acad. Sci. USA 81:11421146.
31. Morooka, T.,, A. Umeda,, and K. Amako. 1985. Motility as an intestinal colonization factor for Campylobacter jejuni. J. Gen. Microbiol. 131:19731980.
32. Nachamkin, I.,, and X. H. Yang. 1988. Isoelectric focusing of Campylobacter jejuni flagellin: micro-heterogeneity and restricted antigenicity of charged species with a monoclonal antibody. FEMS Microbiol. Lett. 49:235238.
33. Newell, D. G. 1986. Monoclonal antibodies directed against the flagella of Campylobacter jejuni: production, characterization and lack of effect on colonization of infant mice. J. Hyg. 96:131141.
34. Newell, D. G.,, H. McBride,, and J. M. Dolby. 1985. Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines. J. Hyg. 95:217227.
35. Nuijten, P. J. M.,, N. M. C. Bleumink-Pluym,, W. Gaastra,, and B. A. M. van der Zeyst. 1989. Flagellin expression in Campylobacter jejuni is regulated at the transcriptional level. Infect. Immun. 57:10841088.
36. Nuyten, P. J. M.,, F. J. A. M. vanAsten,, W. Gaastra,, and B. A. M. van der Zeijst. 1991. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J. Biol. Chem. 265: 1779817804.
37. Nuyten, P. J. M.,, B. A. M. vanderZeijst,, and D. G. Newell. 1991. Localization of immunogenic regions of the flagellin proteins of Campylobacter jejuni 81116. Infect. Immun. 59:11001105.
38. Nuyten, P. J. M.,, T. M. Wassenaar,, D. G. Newell,, and B. A. M. van der Zeyst,. 1992. Molecular characterization and analysis of Campylobacter jejuni flagellin genes and proteins, p. 282296. In I. Nachamkin,, M. J. Blaser,, and L. S. Tompkins (ed.), Campylobacter jejuni: Current Status and Future Trends. American Society for Microbiology, Washington, D.C..
39. Oelschlaeger, T. A.,, P. Guerry,, and D. J. Kopecko. 1993. Unusual microtubule-dependent endocy-tosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. Proc. Natl. Acad. Sci. USA 90:68846888.
40. Oyofo, B. A.,, S. A. Thornton,, D. H. Burr,, T. J. Trust,, and P. Guerry. 1992. Specific detection of Campylobacter jejuni and Campylobacter coli by using the polymerase chain reaction. J. Clin. Microbiol. 30:26132619.
41. Pavlovskis, O. R.,, D. M. Rollins,, R. L. Haberberger, Jr.,, A. E. Green,, L. Habash,, S. Stroko,, and R. I. Walker. 1991. Significance of flagella in colonization resistance of rabbits immunized with Campylobacter spp. Infect. Immun. 59:22592264.
42. Pleier, E.,, and R. Schmitt. 1991. Expression of two Rhizobium meliloti flagellin genes and their contribution to the complex filament structure. J. Bacteriol. 173:20772085.
43. Tauxe, R. V., 1992. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations, p. 919. In I. Nachamkin,, M. J. Blaser,, and L. S. Tompkins (ed.), Campylobacter jejuni: Current Status and Future Trends. American Society for Microbiology, Washington, D.C..
44. Taylor, D. N., 1992. Campylobacter infections in developing countries, p. 2030. In I. Nachamkin,, M. J. Blaser,, and L. S. Tompkins (ed.), Campylobacter jejuni: Current Status and Future Trends. American Society for Microbiology, Washington, D.C..
45. Wang, Y.,, and D. E. Taylor. 1990. Natural transformation in Campylobacter species. J. Bacteriol. 172:949955.
46. Wassenaar, T. M.,, N. M. C. Bleumink-Pluym,, and B. A. M. van der Zeijst. 1991. Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion. EMBO J. 10:20552061.
47. Wassenaar, T. M.,, B. A. M. van der Zevjst,, R. Aryling,, and D. N. Newell. 1993. Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin-A expression. J. Gen. Microbiol. 139:11711175.
48. Yao, R.,, H. Niu,, T. J. Trust,, and P. Guerry. Unpublished data.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error