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Chapter 30 : pJM1 Plasmid-Mediated Iron Transport-Virulence System of Vibrio anguillarum

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Abstract:

causes vibriosis, one of the most devastating diseases affecting salmonid fishes, which results in a hemorrhagic septicemia and death caused by hypoxia and the dysfunction of various organs. The disease caused by this organism has remarkable similarities to invasive septicemic diseases in humans and is thus an interesting model to study bacterial pathogenicity. In many strains of this bacterium, a 65- kb plasmid, pJM1, was identified which encodes an essential component of its virulence repertoire. Mutagenesis experiments of the pJM1 plasmid with TnJ-HoHol led to the identification of six genetic units and identified the iron uptake region to approximately 25 kb of pJM1 DNA. In and most other bacteria, iron forms a ferrous complex with the Fur protein, which acts as a repressor by binding to the Fur-binding sequence found at the promoter region of iron-regulated genes. TAF and AngR are essential as positive enhancers of the expression of anguibactin biosynthesis, at the transcription level, while a chromosomally mediated Fur protein as well as a plasmid-mediated antisense RNAα play roles in the negative control of the expression of the iron transport system. It is remarkable that one of the positive-acting factors, AngR, also shares homology in a motif with members of the firefly luciferase family, of which the DHBA-AMP ligase (EntE) from is a member, and that AngR can replace EntE in mutants and could therefore play a role in the biosynthesis of anguibactin.

Citation: Crosa J. 1994. pJM1 Plasmid-Mediated Iron Transport-Virulence System of Vibrio anguillarum, p 451-461. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch30
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Figure 1

Schematic representation of the pJM1 plasmid-mediated system of V. and the chromosome contribution to its expression, regions containing anguibactin biosynthetic genes; to iron transport genes (is an 86-kDa outer membrane protein receptor, is a ca. 40-kDa periplasmic protein, and and are ca. 40-kDa cytoplasmic membrane proteins); a gene encoding the regulatory (and possibly biosynthetic) protein AngR; TAF, region encoding a r«m.s-acting transcriptional regulator; RNAα, antisense RNA that controls the expression of iron transport genes; Fur, chromosomally mediated protein that, in conjunction with iron, inhibits expression of the pJM1 iron transport and siderophore production systems; DHBA, 2,3-dihydroxybenzoic acid, which is a chromosomally mediated compound that is a precursor of the otherwise pJM1 plasmid-mediated siderophore anguibactin.

Citation: Crosa J. 1994. pJM1 Plasmid-Mediated Iron Transport-Virulence System of Vibrio anguillarum, p 451-461. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch30
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References

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