Chapter 4 : Enterotoxigenic , 1971 to 1979

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This chapter provides a review of the work on enterotoxigenic that was undertaken in Stanley’s lab in the 1970s. Stanley's interest in the extrachromosomal nature of enterotoxin genes was undoubtedly a result of the interplay between his clinical microbiology background and his episome work in a lab studying bacterial gastroenteritis (at the Walter Reed Army Institute of Research). Working on enterotoxin plasmids was therefore a natural extension of these interests. The classic approach was to isolate plasmid mutations and then correlate a particular protein with an LT phenotype (the expression or absence of LT in cells harboring a mutated plasmid). The approach was analogous in a way to the one Maggie had used to identify Ent plasmids in mating experiments when a number of plasmids were present. Nature actually did the first cloning experiment by placing the toxin genes on plasmids, and the authors used the knowledge of plasmids gained by previous students to study these virulence determinants; eventually, they did their own recombinant DNA experiments and cloned the genes to smaller plasmids. The transposon work with Tni led to the development of a plasmid to clone the ST gene, and later, the ST gene was shown to be part of a transposon.

Citation: So M, Dallas W. 1994. Enterotoxigenic , 1971 to 1979, p 55-62. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch4

Key Concept Ranking

Bacterial Pathogenesis
Microbial Genetics
Cholera Toxin
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1. Anderson, E. S. 1965. A rapid screening test for transfer factors in drug sensitive Enterobacteriaceae. Nature (London) 208:10161017.
2. Bazaral, M.,, and D. R. Helinski. 1972. Characterization of multiple circular DNA forms of colicinogenic factor E1 from Proteus mirabilis. Biochemistry 7:35133520.
3. Clements, J. D.,, R. J. Yancey,, and R. A. Finkelstein. 1980. Properties of homogeneous heat-labile enterotoxin from Escherichia coli. Infect. Immun. 29:9197.
4. Cohen, S. N.,, A. C. Y. Chang,, H. W. Boyer,, and R. B. Helling. 1973. Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. USA 70:32403244.
5. Dallas, W. S.,, and S. Falkow. 1979. The molecular nature of heat-labile enterotoxin (LT) of Escherichia coli. Nature (London) 277:406407.
6. Dallas, W. S.,, D. M. Gill,, and S. Falkow. 1979. Cistrons encoding Escherichia coli heat-labile toxin. J. Bacteriol. 139:850858.
7. Dallas, W. S.,, Y.-H. Tseng,, and W. J. Dobrogosz. 1976. Regulation of membrane functions and fatty acid composition in Escherichia coli by cyclic AMP receptor protein. Arch. Biochem. Biophys. 175: 295302.
8. Dougan, G.,, and D. Sheratt. 1977. The transposon Tn1 as a probe for studying Co1E1 structure and function. Mol. Gen. Genet. 151:151160.
9. Gyles, C.,, M. So,, and S. Falkow. 1974. The enterotoxin plasmids of Escherichia coli. J. Infect. Dis. 130:4049.
10. Gyles, C. L. 1971. Heat-labile and heat-stable forms of the enterotoxin from E. coli strains enteropathogenic for pigs. Ann. N. Y. Acad. Sci. 176:314322.
11. Heffron, F.,, P. Bedinger,, J. J. Champoux,, and S. Falkow. 1977. Deletions affecting the transposition of an antibiotic resistance gene. Proc. Natl. Acad. Sci. USA 74:702706.
12. Hershfield, V.,, H. W. Boyer,, C. Yanofsky,, M. A. Lovett,, and D. R. Helinski. 1974. Plasmid Co1E1 as a molecular vehicle for cloning and amplification of DNA. Proc. Natl. Acad. Sci. USA 71: 34553459.
13. Skerman, F. J.,, S. B. Formal,, and S. Falkow. 1972. Plasmid-associated enterotoxin production in a strain of Escherichia coli isolated from humans. Infect. Immun. 5:622624.
14. Smith, H. W. 1968. The transmissible nature of the genetic factor in Escherichia coli that controls enterotoxin production. J. Gen. Microbiol. 52:319334.
15. Smith, H. W.,, and C. L. Gyles. 1970. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J. Med. Microbiol. 3:387401.
16. Smith, H. W.,, and M. A. Linggood. 1971. Observations on the pathogenic properties of the K88 Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J. Med. Microbiol. 4:467485.
17. So, M.,, M. Betlach,, H. Boyer,, and S. Falkow. 1976. The molecular cloning of an Escherichia coli plasmid determinant which encodes the production of heat stable (ST) enterotoxin. J. Bacteriol. 128:463472.
18. So, M.,, J. H. Crosa,, and S. Falkow. 1975. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids. J. Bacteriol. 121:234238.
19. So, M.,, W. S. Dallas,, and S. Falkow. 1978. The molecular cloning of an Escherichia coli plasmid determinant which encodes the production of heat labile (LT) toxin. Infect. Immun. 21:405411.
20. So, M.,, R. Gill,, and S. Falkow. 1975. Generation of a ColEl-Apr cloning vehicle which allows detection of inserted DNA. Mol. Gen. Genet. 142:239249.

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