Chapter 5 : Molecular Epidemiology: Development and Application of Molecular Methods To Solve Infectious Disease Mysteries

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This chapter talks about the author's goal to learn the latest molecular techniques that had been developed during the initial recombinant DNA era and to apply them to a clinically relevant problem. His project was undertaken at the beginning of the recombinant DNA era, and the potential applications of molecular methods to diagnostic microbiology and infectious diseases seemed limitless. Hopkins et al. continued to trace the evolution of antibiotic resistance and discovered that resistance and transmission of resistance markers evolve in very complex ways, demonstrating in a very contemporary way what Louis Pasteur said more than 100 years before the discovery of plasmids and transposons: "The bacteria will always have the last word." Molecular methods are very useful tools for solving epidemiological problems and for detecting and identifying both "old" and "new" pathogens. In addition to their utilities in solving infectious disease "mysteries," in some instances the data derived from these techniques have provided a foundation upon which to approach the study of microbial pathogenesis. This is well illustrated by current studies in the laboratory and in others to investigate the nature of the genes in species encoding pathogenic determinants; these were spawned by molecular epidemiological studies to detect and characterize the infectious agents that cause bacillary angiomatosis and cat scratch disease. As much as this chapter concerns molecular epidemiology, it also reflects the author’s exposure to Stanley's approach to the pathogenesis of infectious diseases.

Citation: Tompkins L. 1994. Molecular Epidemiology: Development and Application of Molecular Methods To Solve Infectious Disease Mysteries, p 63-73. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch5

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Restriction Fragment Length Polymorphism
Infectious Diseases
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Figure 1

Agarose gel electrophoresis of plasmid DNA isolated from methicillin-resistant 5. aureus isolates obtained from patients at SUMC and Palo Alto Veterans Affairs Medical Center (PAVAMC), indicated as VA in the figure. Unique patterns are identified as letters A, B, etc. None of the SUMC strains were genotypically identical to PAVAMC strains, as indicated by unique plasmid profiles, showing that cross-transmission of MRSA from PAVAMC to SUMC did not occur.

Citation: Tompkins L. 1994. Molecular Epidemiology: Development and Application of Molecular Methods To Solve Infectious Disease Mysteries, p 63-73. In Miller V, Kaper J, Portnoy D, Isberg R (ed), Molecular Genetics of Bacterial Pathogenesis. ASM Press, Washington, DC. doi: 10.1128/9781555818340.ch5
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