1887

Chapter 17 : Expression of Foreign Genes in Mycobacteria

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Zoom in
Zoomout

Expression of Foreign Genes in Mycobacteria, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818357/9781555819101_Chap17-1.gif /docserver/preview/fulltext/10.1128/9781555818357/9781555819101_Chap17-2.gif

Abstract:

This chapter reviews the development of genetic systems for the expression of foreign genes in mycobacteria. Mycobacteriophage have been used for many years to type mycobacterial isolates and have more recently been modified as vectors for efficient delivery of foreign DNA into mycobacteria. Plasmid-based expression systems extend the capabilities of phage-based systems by providing increased cloning capacity, increased copy number in the mycobacterial host, and ease of manipulation. Various derivatives of the pAL5000 plasmid replicon have been combined with plasmid replication origins, such as ColEl and pl5A, by several groups to fashion -mycobacterium shuttle vectors. Expression of foreign proteins in recombinant organisms is influenced by many factors. Much of the increased interest in mycobacteria is a result of the emergence of multidrug-resistant . Complex surface macromolecules, such as oligosaccharides and glycolipids, require the products of many genes and the correct cell envelope structure for proper assembly. BCG, the current vaccine against tuberculosis, has provided variable protective efficacy against tuberculosis in different vaccine trials. Immunization of chickens with recombinant expressing an surface antigen elicits partial protection against coccidiosis.

Citation: Burlein J, Offutt S, Hanson M, Stover C. 1994. Expression of Foreign Genes in Mycobacteria, p 239-252. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch17
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Figure 1
Figure 1

Mycobacterial extrachromosomal (pMV261) and integrative (pMV361) expression vectors (modified from ). Common elements in these two vectors include an expression cassette, the Tn905-derived gene (kanamycin resistance), and an origin of replication functional in derived from pUC19. The expression cassette contains 0.4 kb of the 5ʹ end of the BCG gene, including the promoter region, ribosome-binding site, and first six codons (MAKTIA) as well as a multiple cloning site and the transcriptional terminator. The vectors differ by inclusion of either a mycobacterial plasmid replication origin or the and genes of mycobacteriophage L5.

Citation: Burlein J, Offutt S, Hanson M, Stover C. 1994. Expression of Foreign Genes in Mycobacteria, p 239-252. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch17
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 2
Figure 2

Expression of foreign antigens in different BCG substrains. BCG culture lysates equivalent to approximately 5 x 106 bacteria were analyzed by SDS-PAGE and Coomassie blue staining (A) or by immunoblotting with antibodies specific for foreign antigens OspA (B, C) or HIV-1 gpl20 (D). Panel A shows the total proteins expressed by nonrecombinant BCG. Panels B through D show relevant portions of immunoblots of different BCG recombinants expressing the indicated foreign antigens.

Citation: Burlein J, Offutt S, Hanson M, Stover C. 1994. Expression of Foreign Genes in Mycobacteria, p 239-252. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch17
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555818357.chap17
1. Aldovini, A.,, R. N. Husson,, and R. A. Young. 1993. The uraA locus and homologous recombination in Mycobacterium bovis BCG. J. Bacterial. 175: 7282 7289.
2. Aldovini, A.,, and R. A. Young. 1991. Humoral and cell-mediated responses to live recombinant BCG-HIV vaccines. Nature (London) 351: 479 482.
3. Andersen, A. B.,, and E. B. Hansen. 1989. Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular weight protein of Mycobacterium tuberculosis. Infect. Immun. 57: 2481 2488.
4. Ashbridge, K. R.,, R. J. Booth,, J. D. Watson,, and R. Lathigra. 1989. Nucleotide sequence of the 19 kDa antigen gene from Mycobacterium tuberculosis. Nucleic Acids Res. 17: 1249.
5. Banerjee, A.,, E. Dubnau,, A. Quemard,, V. Balasubra-manian,, K. S. Um,, T. Wilson,, G. de Lisle,, and W. R. Jacobs Jr.. 1994. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science 263: 227 230.
6. Barletta, R. G.,, D. D. Kim,, S. B. Snapper,, B. R. Bloom,, and W. R. Jacobs Jr.. 1992. Identification of expression signals of the mycobacteriophages Bxbl, LI, and TM4 using the Escherichia-mycobacterium shuttle plasmids pYUB75, and pYUB76 designed to create translational fusions to the lacZ gene. J. Gen. Microbiol. 138: 23 30.
7. Belisle, J. T.,, L. Pascopella,, J. M. Inamine,, P. J. Brennan,, and W. R. Jacobs Jr.. 1991. Isolation and expression of a gene cluster responsible for biosynthesis of the glycopeptidolipid antigens of Mycobacterium avium. J. Bacteriol. 173: 6991 6997.
8. Bergstrom, S.,, V. G. Bundoc,, and A. G. Barbour. 1989. Molecular analysis of linear* plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi. Mol. Microbiol. 3: 479 486.
9. Borremans, M.,, L. de Wit,, G. Volckaert,, J. Ooms,, J. de Bruyn,, K. Huygen,, J.-P. van Vooren,, M. Stelandre,, R. Verhofstadt,, and J. Content. 1989. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis. Infect. Immun. 57: 3123 3130.
10. Buchmeier, N. A.,, and F. Heffron. 1990. Induction of Salmonella stress proteins upon infection of macrophages. Science 248: 730 732.
11. Buell, G.,, and N. Panayotatos,. 1986. Mechanism and practice, p. 345 363. In W. Reznikoff, and L. Gold (ed.), Maximizing Gene Expression. Butterworths, Boston.
12. Cassatt, D. R.,, V. F. de la Cruz,, J. E. Burlein,, J. Young,, S. Koenig,, and C. Kendall Stover,. 1993. Protection of mice against tetanus challenge using an experimental tetanus vaccine based on recombinant BCG, p. 385 389. In H. S. Ginsberg,, F. Brown,, R. M. Channock,, and R. A. Lerner (ed.), Vaccines 93. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
13. Clark-Curtiss, J. E.,, W. R. Jacobs,, M. A. Docherty,, L. R. Ritchie,, and R. Curtiss. 1985. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae. J. Bacteriol. 161: 1093 1102.
14. Connell, N. D.,, E. Medina-Acosta,, W. R. McMaster,, B. R. Bloom,, and D. G. Russell. 1993. Effective immunization against cutaneous leishmaniasis with recombinant BCG expressing the Leishmania surface proteinase (gp63). Proc Natl Acad. Sci. USA 90: 11473 11477.
15. Content, J.,, A. de la Cuvellerie,, L. de Wit,, V. Vincent-Levy-Frebault,, J. Ooms,, and J. de Bruyn. 1991. The gene coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. Infect. Immun. 59: 3205 3212.
16. Convit, J.,, N. Aranzazu,, M. Ulrich,, M. E. Pinardi,, O. Reyes,, and J. Alvarado. 1982. Immunotherapy with a mixture of Mycobacterium leprae and BCG in different forms of leprosy and Matsuda negative contacts. Int. J. Lepr. 50: 415.
17. Cooksey, R. C, J. T. Crawford, W. R. Jacobs, and T. M. Shinnick. 1993. A rapid method for screening antimicrobial agents for activity against a strain of Mycobacterium tuberculosis expressing firefly luciferase. Antimicrob. Agents Chemother. 37: 1348 1352.
18. Dale, J. W.,, and A. Patki. 1990. Mycobacterial gene expression and regulation, p. 173 198. InJ. McFadden (ed.), Molecular Biology of the Mycobacteria. Surrey University Press, London.
19. de Boer, H. A.,, and R. A. Kastelein,. 1986. Biased codon usage: an exploration of its role in optimization of translation, p. 225 286. In W. Reznikoff, and L. Gold (ed.), Maximizing Gene Expression. Butterworths, Boston.
20. Donnelly-Wu, M. K.,, W. R. Jacobs Jr.,, and G. F. Hatfull. 1993. Superinfection immunity of mycobacteriophage LS: applications for genetic transformation of mycobacteria. Mol. Microbiol. 7: 407 417.
21. Finlay, B. B.,, and S. Falkow. 1989. Common themes in microbial pathogenicity. Microbiol. Rev. 53: 210 230.
22. Fortier, A. H.,, B. A. Mock,, M. S. Meitzer,, and C. A. Nacy. 1987. Mycobacterium bovis BCG-induced protection against cutaneous and systemic Leishmania major infections of mice. Infect. Immun. 55: 1707 1714.
23. Galen, J.. Unpublished data.
24. Garbe, T.,, D. Harris,, M. Vordermeier,, R. Lathigra,, J. Ivanyi,, and D. Young. 1993. Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: immunological analysis and evidence of glycosylation. Infect. Immun. 61: 260 267.
25. Goldberg, A., L-, and S. A. Goff. 1986. The selective degradation of abnormal proteins in bacteria, p. 287 314. In W. Reznikoff, and L. Gold (ed.), Maximizing Gene Expression. Butterworths, Boston.
26. Goldberg, M. B.,, S. A. Boyko,, and S. B. Calderwood. 1990. Transcriptional regulation by iron of a Vibrio cholerae virulence gene and homology of the gene to the Escherichia coli Fur system. J. Bacteriol. 172: 6863 6870.
27. Haeseleer, F.,, J.-F. Pollet,, M. Haumont,, C. Dubeaux,, A. Bollen,, P. Jacobs,, M. Modesti,, and J. Cohen,. 1993. Stable integration and expression of Plasmodium falciparum antigen coding sequences in mycobacteria, p. 413 417. In H. S. Ginsberg,, F. Brown,, R. M. Channock,, and R. A. Lerner (ed.), Vaccines 93. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
28. Hanson, M. S.,, C. V. Lapcevich,, and S. L. Haun. Progress on development of the live BCG recombinant (rBCG) vaccine vehicle for combined delivery. Ann. N.Y. Acad. Sci., in press.
29. Hatful), G. F.,, and G. J. Sarkis. 1993. DNA sequence, structure, and gene expression of mycobacterio-phage L5: a phage system for mycobacterial genetics. Mol. Microbiol. 7: 395 405.
30. Hermans, J.,, C. Martin,, G. N. M. Huijberts,, T. Goosen,, and J. A. M. de Bont. 1991. Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range gram-negative cosmid vector pJRD215. Mol. Microbiol. 5: 1561 1566.
31. Hull, S. I.,, R. J. WaUace Jr.,, D. G. Bobey,, K. E. Price,, R. A. Goodhines,, J. M. Swenon,, and V. A. Silcox. 1984. Presence of aminoglycoside acetyltransferase and plasmids in Mycobacterium fortuitum. Am. Rev. Respir. Dis. 129: 614 618.
32. Jacobs, W. R. Jr.,, R. G. Barletta,, R. Udani,, J. Chan,, G. Kalkut,, G. Sosne,, T. Keiser,, G. J. Sartas,, G. F. Hatfull,, and B. R. Bloom. 1993. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science 260: 819 822.
33. Jacobs, W. R. Jr.,, G. V. Kalpana,, J. D. Cirillo,, L. Pascopella,, S. B. Snapper,, R. A. Udani,, W. Jones,, R. G. Barletta,, and B. R. Bloom. 1991. Genetic systems for mycobacteria. Methods Enzymol. 204: 537 555.
34. Jacobs, W. R. Jr.,, M. Tuckman,, and B. R. Bloom. 1987. Introduction of foreign DNA into mycobacteria using a shuttle phasmid. Nature (London) 327: 532 535.
35. Jenkins, M. C.,, and H. S. Lillehoj. Personal communication.
36. Kalpana, G. V.,, B. R. Bloom,, and W. R. Jacobs Jr.. 1991. Insertional mutagenesis and illegitimate recombination in mycobacteria. Proc. NatL Acad. Sci. USA 88: 5433 5437.
37. Labidi, A.,, H. L. David,, and D. Roulland-Dussoix. 1985. Cloning and expression of mycobacterial plasmid DNA in Escherichia coli. FEMS Microbiol. Lett. 30: 221 225.
38. Lanar, D.. Unpublished data.
39. Lathigra, R., et al. Unpublished data.
40. Lazraq, R.,, S. Clavel-Seres,, and H. L. David. 1991. Transformation of distinct mycobacterial species by shuttle vectors derived from the Mycobacterium fortuitum pAL5000 plasmid. Curr. Microbiol. 22: 9 13.
41. Lazraq, R.,, S. Clavel-Seres,, H. L. David,, and D. Roulland-Dussoix. 1990. Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis. FEMS Microbiol. Lett. 69: 135 138.
42. Lee, C.,, P. Li,, H. Inouye,, E. Brickman,, and J. Beckwith. 1989. Genetic studies on the inability of β-ga-lactosidase to be translocated across the Escherichia coli cytoplasmic membrane. J. Bacterial. 171: 4609 4616.
43. Lee, M. H.,, L. Pascopella,, W. R. Jacobs Jr.,, and G. F. Hatfull. 1991. Site-specific integration of mycobac-teriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis and bacille Calmette-Guerin. Proc. Natl. Acad. Sei. USA 88: 3111 3115.
44. Martin, C.,, J. Timm,, J. Rauzier,, R. Gomez-Lus,, J. Davies,, and B. Gicquel. 1990. Transposition of an antibiotic resistance element in mycobacteria. Nature (London) 345: 739 743.
45. Matsuo, K.,, R. Yamaguchi,, A. Yamazata,, H. Tasaka,, K. Terasaka,, M. Totsuka,, K. Kobayashi,, H. Yukitake,, and T. Yamada. 1990a. Establishment of a foreign secretion system in mycobacteria. Infect. Immun. 58: 4049 4054.
46. Matsuo, K.,, R. Yamaguchi,, A. Yamazaki,, H. Tasaka,, K. Terasaka,, and T. Yamada. 1990b. Cloning and expression of the gene for the cross-reactive a antigen of Mycobacterium kansasii. Infect. Immun. 58: 550 556.
47. Mehra, V.,, D. Sweetser,, and R. A. Young. 1986. Efficient mapping of protein antigen determinants. Proc. Natl. Acad. Sci. USA 83: 7013 7017.
48. Meissner, P. S.,, and J. O. Falkingham. 1984. Plasmid-encoded mercuric reductase in Mycobacterium scrofulaceum. J. Bacterial. 157: 669 672.
49. Miller, J. F.,, C. R. Roy,, and S. Falkow. 1989. Analysis of Bordetella pertussis virulence gene regulation by use of transcriptional fusions in Escherichia coli. J. Bacteriol 171: 6345 6348.
50. Murray, A.,, N. Winter,, M. Lagranderie,, D. F. Hill,, J. Rauzier,, J. Timm,, C. Leclerc,, K. M. Moriarty,, M. Gheorghiu,, and B. Gicquel. 1992. Expression of Escherichia coli β-galactosidase in Mycobacterium bovis BCG using an expression system from Mycobacterium paratuberculosis which induced humoral and cellular immune responses. Mol. Microbiol. 6: 3331 3342.
51. Nair, J.,, D. A. Rouse,, and S. L. Morris. 1992. Nucleotide sequence analysis and serologic characterization of the Mycobacterium intracelhilare homologue of the Mycobacterium tuberculosis 19 kDa antigen. Mol Microbiol. 6: 1431 1439.
52. Nakayama, K.,, S. M. Kelly,, and R. Curtiss IH. 1988. Construction of an Asd + expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmonella vaccine vector strain. Bio/Technology 6: 693 697.
53. Osborne, T. W. 1983. Changes in BCG strains. Tubercle 64: 1 132.
54. Park, S. F.,, G. S. A. B. Stewart,, and R. G. Kroll. 1992. The use of bacterial luciferase for monitoring the environmental regulation of expression of genes encoding virulence factors in Listeria monocytogenes. /. Gen. Microbiol. 138: 2619 2627.
55. Pearce, E. J.,, S. L. James,, S. Hierny,, D. E. Lanar,, and A. Sher. 1988. Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen. Proc. Natl. Acad. Sei. USA 85: 5679 5682.
56. Radford, A. J.,, and A. L. M. Hodgson. 1991. Construction and characterization of a mycobacterium- Escherichia coli shuttle vector. Plasmid 25: 149 153.
57. Ranes, M. G.,, J. Rauzier,, M. Lagranderie,, M. Gheorghiu,, and B. Gicquel. 1990. Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium- Escherichia coli shuttle vector. J. Bacteriol 172: 2793 2797.
58. Schein, C. 1989. Production of soluble recombinant proteins in bacteria. Bio/Technology 7: 1141 1149.
59. Shinnick, T. M. 1987. The 65-kilodalton antigen of Mycobacterium tuberculosis. J. Bacteriol. 169: 1080 1088.
60. Snapper, S. B.,, L. Lugosi,, A. Jekkel,, R. Melton,, T. Kieser,, B. R. Bloom,, and W. R. Jacobs Jr.. 1988. Lysogeny and transformation of mycobacteria: stable expression of foreign genes. Proc. Natl. Acad. Sci. USA 85: 6987 6991.
61. Stover, C. K.,, G. P. Bansal,, M. S. Hanson,, J. E. Burlein,, S. R. Palaszynski,, J. F. Young,, S. Koenig,, D. B. Young,, A. Sadziene,, and A. Barbour. 1993a. Protective immunity elicited by a recombinant bacille Calmette-Guerin (BCG) expressing outer surface protein A (OspA) lipoprotein: a candidate Lyme disease vaccine. J. Exp. Med. 178: 197 209.
62. Stover, C. K.,, V. F. de la Cruz,, T. R. Fuerst,, J. E. Burlein,, L. A. Benson,, L. T. Bennett,, G. P. Bansal,, J. F. Young,, M. H. Lee,, G. F. Hatfull,, S. B. Snapper,, R. G. Barletta,, W. R. Jacobs Jr.,, and B. R. Bloom. 1991. New use of BCG for recombinant vaccines. Nature (London) 351: 458 460.
63. Stover, C. K.,, S. Langermann,, M. S. Hanson,, J. E. Burlein,, S. R. Palaszynski,, S. Koenig,, L. S. McDaniel,, and D. E. Briles. 1993b. Candidate pneumococcal vaccines based on the recombinant BCG vaccine vehicle, abstr. E-79. Abstr. 93rd Gen. Meet. Am. Soc. Microbiol. 1993.
64. Thole, J. E.,, H. G. Dauwerse,, P. K. Das,, D. G. Groothuis,, L. M. Schouls,, and J. D. A. van Embden. 1985. Cloning of Mycobacterium bovis DNA and expression of antigens in Escherichia coli. Infect. Immun. 50: 800 806.
65. Thole, J. E. R.,, R. Schoningh,, A. A. M. Janson,, T. Garbe,, Y. E. Cornelisse,, J. E. Clark-Curtiss,, A. H. J. Kolk,, T. H. M. Ottenhoff,, R. R. R. De Vries,, and C. Abou-Zeid. 1992. Molecular and immunological analysis of a fibronectin-binding protein antigen secreted by Mycobacterium leprae. Mol. Microbiol. 6: 153 163.
66. Wada, K.,, Y. Wada,, F. Ishibashi,, T. Gojobori,, and T. Ikemura. 1992. Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res. 20: S2111 S2118.
67. Winter, N.,, M. Lagranderie,, J. Rauzier,, J. Timm,, C. Leclerc,, B. Guy,, M. P. Kieny,, M. Gheorghiu,, and B. Gicquel. 1991. Expression of heterologous genes in Mycobacterium bovis BCG: induction of a cellular response against HIV-1 Nef protein. Gene 109: 47 54.
68. Yasutomi, Y.,, S. Koenig,, S. S. Haun,, C. K. Stover,, R. K. Jackson,, P. Conrad,, A. J. Conley,, E. A. Emini,, T. R. Fuerst,, and N. L. Letvin. 1993. Immunization with recombinant BCG-SIV elicits SIV-specific cytotoxic T lymphocytes in rhesus monkeys. J. Immunol. 150: 3101 3107.
69. Young, D. B.,, and T. R. Garbe. 1991. Lipoprotein antigens of Mycobacterium tuberculosis. Res. Microbiol. 142: 55 65.
70. Young, R. A.,, B. R. Bloom,, C. M. Grosskinsky,, J. Ivanyi,, D. Thomas,, and R. W. Davis. 1985a. Dissection of Mycobacterium tuberculosis antigens using recombinant DNA. Proc. Natl. Acad. Sci. USA 82: 2583 2587.
71. Young, R. A.,, V. Mehra,, D. Sweetser,, T. Buchanan,, J. Clark-Curtiss,, R. W. Davis,, and B. R. Bloom. 1985b. Genes for the major protein antigens of the leprosy parasite, Mycobacterium leprae. Nature (London) 316: 450 452.
72. Zhang, Y.,, B. Heym,, B. Allen,, D. Young,, and S. Cole. 1992. The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis. Nature (London) 358: 591 593.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error