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Chapter 19 : Ultrastructure of

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Abstract:

was an early object of study by electron microscopy, consonant with its importance as a human pathogen. This chapter describes what is understood of the ultrastructure of and points out problems of interpretation and areas where further investigation, probably involving the development of new techniques, is needed. Most bacteria do not possess the elaborate system of internal compartments found in eukaryotic cells, and the information obtained about mycobacteria by electron microscopy is primarily information about the envelope layers, a discussion of which forms the major part of the chapter. It also describes the interaction between bacterium and host cells and some aspects of the ultrastructure of this interaction. Membranes of mycobacteria, including , appear in ultrathin sections as classic bilayers, with two electrondense layers separated by a transparent layer. If the hypothesis is that the walls of and other mycobacteria form permeability barriers somewhat analogous to the outer membranes of gram-negative bacteria, then the space between the outer leaflet of the membrane and the wall forms a compartment analogous to the periplasmic space of gram-negative bacteria. The commonest type of host cell is the macrophage; inside this cell, the bacteria occur within vacuoles, apparently the phagosomes formed as the bacteria are engulfed by the cells.

Citation: Brennan P, Draper P. 1994. Ultrastructure of , p 271-284. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch19
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Figures

Image of Figure 1.
Figure 1.

Appearance of mycobacterial envelope in thin sections. (A) Electron micrograph of envelope and part of cell contents of 425. Cells were fixed by freeze-substitution ( ) to optimize preservation of structure and to reduce extraction of lipid components by solvents used in processing. (Photograph kindly supplied by Terry Paul, University of Guelph, Guelph, Ontario, Canada.) Bar indicates 30 nm. (B) Interpretation of image shown in panel A in terms of layer structure described in text. Thickness of layers is enlarged about twofold compared with thickness in the micrograph. Modified from Fig. 15 of Paul and Beveridge (1992).

Citation: Brennan P, Draper P. 1994. Ultrastructure of , p 271-284. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch19
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Image of Figure 2.
Figure 2.

Model of the structure of the mycobacterial cell envelope. Positions of the asymmetric plasma membrane, peptidoglycan, and covalently attached arabinogalactan are indicated, together with LAM and phosphatidylinositol mannosides (PIM), at least some of which are known to be associated with the plasma membrane. A possible arrangement of the wall-associated lipids is also shown. Mycolic acids residues are known to be attached to the majority of the terminal and penultimate arabinose residues of the arabinogalactan. Since the mycolates possess two hydrocarbon chains of unequal lengths that would form an irregular monolayer, it is proposed that these are complemented by two classes of polar lipids with medium-chain (e.g., mycocerosates) and short-chain (e.g., acylglycerols) fatty acyl chains, respectively. There is evidence for a small number of porins in the envelope, presumably within the outer hydrophobic bilayer. Adapted from Minnikin (1982), McNeil and Brennan (1991), and Nikaido et al. (1993).

Citation: Brennan P, Draper P. 1994. Ultrastructure of , p 271-284. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch19
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References

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1. Andres, Y.,, H. J. MacCordick,, and J. C. Hubert. 1993. Adsorption of several actinide (Th, U) and lanthanide (La, Eu, Yb) ions by Mycobacterium smegmatis. Appl. Microbiol. Biotechnol. 39:413417.
2. Armstrong, J. A.,, and P. D. Hart. 1971. Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. J. Exp. Med. 134:713740.
3. Barksdale, L.,, and K. S. Kim. 1977. Mycobacterium. Bacteriol. Rev. 41:217372.
4. Barrow, W. W.,, and P. J. Brennan. 1982. Isolation in high frequency of rough variants of Mycobacterium intracellulare lacking C-mycoside glycopeptidolipid antigens. J. Bacteriol. 150:381384.
5. Beveridge, T. J.,, and R. G. E. Murray. 1980. Sites of metal deposition in the cell wall of Bacillus subtilis. J. Bacteriol. 141:876887.
6. Binkhuysen, F.,, and P. K. Das. 1982. Ultrastructural characteristics of Mycobacterium bovis BCG and Mycobacterium leprae. Int. J. Lepr. 50:7682.
7. Brodie, A. F.,, V. K. Kalra,, S. H. Lee,, and N. S. Cohen. 1979. Properties of energy-transducing systems in different types of membrane preparations from Mycobacterium phlei—preparation, resolution, and reconstitution. Methods Enzymol. 55:175200.
8. Daffé, M.,, M. A. Dupont,, and N. Gas. 1989. The cell envelope of Mycobacterium smegmatis: cytochemistry and architectural implications. FEMS Microbiol. Lett. 61:8994.
9. Draper, P. 1971. The walls of Mycobacterium leprae-murium: chemistry and ultrastructure. J. Gen. Microbiol. 69:313324.
10. Draper, P. 1974. The mycoside capsule of Mycobacterium avium 357. J. Gen. Microbiol. 83:431433.
11. Draper, P., 1982. The anatomy of mycobacteria, p. 952. In C. Ratledge, and J. Stanford (ed.), The Biology of the Mycobacteria, vol. 1. Physiology, Identification and Classification. Academic Press, Inc., London.
12. Edwards, R. P. 1970. Electron-microscope illustrations of division in Mycobacterium leprae. J. Med. Microbiol. 3:493499.
13. Engers, H. D.,, M. Abe,, B. R. Bloom,, V. Mehra,, W. Britton,, T. M. Buchanan,, S. K. Khanolkar,, D. B. Young,, O. Closs,, T. Gillis,, M. Harboe,, J. Ivanyi,, A. H. J. Kolk,, and C. C. Shepard. 1985. Results of a World Health Organization-sponsored workshop on monoclonal antibodies to Mycobacterium leprae. Infect. Immun. 48:603605.
14. Frehel, C.,, A. Ryter,, N. Rastogi,, and H. David. 1986. The electron-transparent zone in phagocytosed Mycobacterium avium and other mycobacteria: formation, persistence and role in bacterial survival. Ann. Inst. PasteurlMicrobiol. 137B:239257.
15. Good, R. C.,, and R. E. Beam,. 1984. Seroagglutination, p. 105122. In G. P. Kubica, and L. G. Wayne (ed.), The Mycobacteria—a Sourcebook, part A. Marcel Dekker, Inc., New York.
16. Goren, M., and P. J. Brennan,. 1979. Mycobacterial lipids: chemistry and biologic activities, p. 63193. In G. P. Youmans (ed.), Tuberculosis. The W.B. Saunders Co., Philadelphia.
17. Hayat, M. A. 1989. Principles and Techniques of Electron Microscopy, 3rd ed. Macmillan, Basingstoke.
18. Hirata, T. 1979. Electron microscopic observations of intracytoplasmic membrane systems and cell division in Mycobacterium lepraemurium. Int. J. Lepr. 47:585596.
19. Hirschfleld, G. R.,, M. McNeil,, and P. J. Brennan. 1990. Peptidoglycan-associated polypeptides of Mycobacterium tuberculosis. J. Bacteriol. 172:10051013.
20. Hunter, S. W.,, H. Gaylord,, and P. J. Brennan. 1986. Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. J. Biol. Chem. 261:1234512351.
21. Imaeda, T.,, F. Kanetsuna,, and B. Galindo. 1968. Ultrastructure of cell walls of genus Mycobacterium. J. Ultrastruct. Res. 25:4663.
22. Kumar, G.,, V. K. Kalra,, and A. F. Brodie. 1979. Asymmetric distribution of phospholipids in membranes from Mycobacterium phlei. Arch. Biochem. Biophys. 198:2230.
23. Lederer, E.,, A. Adam,, R. Ciorbaru,, J. F. Petit,, and J. Wietzerbin. 1975. Cell walls of mycobacteria and related organisms; chemistry and immunostimulant properties. Mol. Cell. Biochem. 7:87104.
24. Lounatmaa, K.,, and E. Brander. 1989. Crystalline surface layer of Mycobacterium bovis BCG. J. Bacteriol. 171:57565758.
25. McDonough, A.,, V. Kress,, and B. R. Bloom. 1993. Pathogenesis of tuberculosis: interaction of Mycobacterium tuberculosis with macrophages. Infect. Immun. 61:27632773.
26. McNeil, M.,, M. Daffé,, and P. J. Brennan. 1990. Evidence for the nature of the link between the arabi-nogalactan and peptidoglycan of mycobacterial cell walls. J. Biol. Chem. 265:1820018206.
27. McNeil, M. R.,, and P. J. Brennan. 1991. Structure, function and biogenesis of the cell envelope of mycobacteria in relation to bacterial physiology, pathogenesis and drug resistance; some thoughts and possibilities arising from recent structural information. Res. Microbiol. 142:451463.
28. Messner, P.,, and U. B. Sleytr. 1992. Crystalline bacterial cell-surface layers. Adv. Microb. Physiol. 33: 213275.
29. Minnikin, D. E., 1982. Lipids: complex lipids, their chemistry, biosynthesis and roles, p. 95184. In C. Ratledge, and J. Stanford (ed.), The Biology of the Mycobacteria, vol. 1. Physiology, Identification and Classification. Academic Press, Inc., London.
30. Nguyen, H. T.,, D. D. Trach,, N. V. Man,, T. H. Ngoan,, I. Dunia,, M. A. Ludosky-Diawara,, and E. L. Bene-detti. 1979. Comparative ultrastructure of Mycobacterium leprae and Mycobacterium lepraemurium cell envelopes. J. Bacteriol. 138:552558.
31. Nikaido, H.,, S. H. Kim,, and E. Y. Rosenberg. 1993. Physical organization of lipids in the cell wall of Mycobacterium chelonae. Mol. Microbiol. 8:10251030.
32. Nishiura, M.,, S. Izumi,, T. Mori,, K. Takeo,, and T. Nonaka. 1977. Freeze-etching study of human and murine leprosy bacilli: Int. J. Lepr. 45:248254.
33. Nishiura, M.,, S. Okada,, S. Izumi,, and H. Takizawa. 1969. An electron microscope study of the band structure of the leprosy bacillus and other mycobacteria. Int. J. Lepr. 37:225238.
34. Paul, T. R.,, and T. J. Beveridge. 1992. Réévaluation of envelope profiles and cytoplasmic ultrastructure of mycobacteria processed by conventional embedding and freeze-substitution protocols. J. Bacteriol. 174: 65086517.
35. Rastogi, N. 1991. Recent observations concerning structure and function relationships in the mycobacterial cell envelope: elaboration of a model in terms of mycobacterial pathogenicity, virulence and drug-resistance. Res. Microbiol. 142:464476.
36. Rastogi, N., and H. L. David. 1981. Growth and cell division of Mycobacterium avium. J. Gen. Microbiol. 126:7784.
37. Rastogi, N.,, C. Frehel,, and H. L. David. 1984. Evidence for taxonomie utility of periodic acid-thiocarbohydrazide-silver proteinate cytochemical staining for electron microscopy. Int. J. Syst. Bacteriol. 34:293299.
38. Rastogi, N.,, C. Frehel,, and H. L. David. 1986. Triplelayered structure of mycobacterial cell wall: evidence for the existence of a polysaccharide-rich outer layer in 18 mycobacterial species. Curr. Microbiol. 13:237242.
39. Rosenblatt, M.,, E. F. Fullam,, and A. E. Gessler. 1942. Studies of mycobacteria with the electron microscope. Am. Rev. Tuberc. 46:587599.
40. Silva, M. T.,, and P. M. Macedo. 1983a. The interpretation of the ultrastructure of mycobacterial cells in transmission electron microscopy of ultrathin sections. Int. J. Lepr. 51:225234.
41. Silva, M. T.,, and P. M. Macedo. 1983b. A comparative ultrastructural study of the membranes of Mycobacterium leprae and of cultivable mycobacteria. Biol. Cell. 47:383386.
42. Takade, A.,, K. Takeya,, H. Taniguchi,, and Y. Mizugu-chi. 1983. Electron microscopic observations of cell division in Mycobacterium vaccae VI. J. Gen. Microbiol. 129:23152320.
43. Takeya, K.,, R. Mori,, M. Koike,, and T. Toda. 1958. Paired fibrous structures in mycobacteria. Biochim. Biophys. Acta 30:197198.
44. Takeya, K.,, R. Mori,, T. Tokunaga,, M. Koike,, and K. Hisatsune. 1961. Further studies on the paired fibrous structures of mycobacterial cell wall. J. Biophys. Biochem. Cytol. 9:496501.
45. Trias, J.,, and R. Benz. 1993. Characterization of the channel formed by the mycobacterial porin in lipid bilayer membranes. Demonstration of voltage gating and of negative point charges at the channel mouth. J. Biol. Chem. 268:62346240.
46. Trias, J.,, V. Jarlier,, and R. Benz. 1992. Porins in the cell wall of mycobacteria. Science 258:14791481.
47. Wayne, L. G. 1977. Synchronized replication of Mycobacterium tuberculosis. Infect. Immun. 17:528530.
48. Wiker, H. G.,, M. Harboe,, and S. Nagai. 1991. A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis. J. Gen. Microbiol. 137:875884.

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