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Chapter 30 : Molecular Approaches to the Diagnosis of Tuberculosis

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Molecular Approaches to the Diagnosis of Tuberculosis, Page 1 of 2

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Abstract:

This chapter reviews the progress in developing new tools and procedures for the rapid and reliable diagnosis of tuberculosis. A definitive diagnosis of tuberculosis requires the identification of bacilli in patient specimens. A definitive diagnosis of tuberculosis requires the identification of bacilli in patient specimens. In general, the commercially available assays display sensitivities and specificities approaching 100% for the detection and identification of these species and can usually be completed in a few hours. One easily detected component of is tuberculostearic acid, which can be detected in femtomole quantities by gas-liquid chromatography. Several diagnositic procedures have been described for use with and include strand displacement amplification (SDA), polymerase chain reaction (PCR) amplification, transcription-mediated amplification (TMA), reporter phage systems, oligonucleotide ligation amplification, and Q-beta replicase amplification. If one uses a physician’s diagnosis of tuberculosis as the gold standard, then the MTD test displayed 95% sensitivity and 96% specificity for correctly identifying persons as having tuberculosis. Given the importance of drug-resistant tuberculosis, it would be advantageous to determine drug susceptibilities directly from clinical specimens and avoid the time required for culture. The luciferase reporter technology has been used to develop simple and rapid screening procedures to identify new antituberculosis agents.

Citation: Shinnick T, Jonas V. 1994. Molecular Approaches to the Diagnosis of Tuberculosis, p 517-530. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch30
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References

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1. Altamirano, M.,, M. T. Kelly,, A. Wong,, E. T. Bessuille,, W. A. Black,, and J. A. Smith. 1992. Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction. J. Clin. Microbiol. 30: 21732176.
2. American Thoracic Society and Centers for Disease Control. 1981. The tuberculin skin test. Am. Rev. Respir. Dis. 124:356363.
3. American Thoracic Society and Centers for Disease Control. 1990. Diagnostic standards and classification of tuberculosis. Am. Rev. Respir. Dis. 142:725735.
4. Banerjee, A.,, E. Dubnau,, A. Quemard,, V. Balasubra-manian,, K. S. Urn,, T. Wilson,, D. Collins,, G. de Lisle,, and W. R. Jacobs, Jr. 1994. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science 263:227230.
5. Bates, J.,, P. J. Brennan,, G. W. Douglas,, J. C. Feeley,, J. Glassroth,, D. E. Kohne,, W. J. Martin,, L. G. Wayne,, and, C. R. Zeiss. 1986. Subcommittee report on improvements in the diagnosis of tuberculosis. Am. Rev. Respir. Dis. 134(Suppl.):415417.
6. Böddinghaus, B.,, T. Rogall,, T. Flohr,, H. Blöcker,, and E. C. Bouger. 1990. Detection and identification of mycobacteria by amplification of rRNA. J. Clin. Microbiol. 28:17511759.
7. Brisson-Noel, A.,, B. Gicquel,, D. Lecossier,, V. Levy-Frebault,, X. Nassif,, and A. J. Hance. 1989. Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples. Lancet ii:10691071.
8. Brooks, J.,., M. I. Daneshvar,, D. M. Fast,, and R. C. Good. 1987. Selective procedures for detecting femtomole quantities of tuberculostearic acid in serum and cerebrospinal fluid by frequency-pulsed electron-capture gas-liquid chromatography. J. Clin. Microbiol. 25:12011206.
9. Brooks, J.,, M. I. Daneshvar,, R. L. Haberberger,, and I. A. Mikhail. 1990. Rapid diagnosis of tuberculous meningitis by frequency-pulsed electron-capture gas-liquid chromatography detection of carboxylic acids in cerebrospinal fluid. J. Clin. Microbiol. 28:989997.
10. Butler, W. R.,, and J. O. Kilburn. 1988. Identification of major slow growing pathogenic mycobacteria and Mycobacterium gordonaeby high-performance liquid chromatography of their mycolic acids. J. Clin. Microbiol. 26:5053.
11. Centers for Disease Control. 1990. The use of preventive therapy for tuberculosis infection in the United States. Recommendations of the Advisory Committee for the Elimination of Tuberculosis. Morbid. Mortal. Weekly Rep. 39(RR-8):912.
12. Centers for Disease Control. 1991. Purified protein derivative (PPD)-tuberculin anergy and HIV infection: guidelines for anergy testing and management of anergic persons at risk of tuberculosis. Morbid. Mortal. Weekly Rep. 40:2733.
13. Clarridge, J. E.,, R. M. Shawar,, T. M. Shinnick,, and B. Plikaytis. 1993. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. J. Clin. Microbiol. 31:20492056.
14. Cooksey, R.C.,, J. T. Crawford,, W. R. Jacobs, Jr.,, and T. M. Shinnick. 1993. A rapid method for screening antimicrobial agents for activity against a strain of Mycobacterium tuberculosis expressing firefly luciferase. Antimicrob. Agents Chemother. 37:13481352.
15. Cousins, D. V.,, S. D. Wilton,, B. R. Francis,, and B. L. Gow. 1992. Use of polymerase chain reaction for rapid diagnosis of tuberculosis. J. Clin. Microbiol. 30:255258.
16. Czerkinsky, C.,, L. A. Nilsson,, H. Nygren,, O. Ouchterlony,, and A. Tarkowski. 1983. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Methods 65:109121.
17. Del Portillo, P.,, L. A. Murillo,, and M. E. Patarroyo. 1991. Amplification of species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis. J. Clin. Microbiol. 29:21632168.
18. de Wet, J. R.,, V. Wood,, M. DeLuca,, D. R. Helinski,, and S. Subramani. 1987. Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell. Biol. 7:725737.
19. DeWit, D.,, L. Steyn,, S. Shoemaker,, and M. Sogin. 1990. Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification. J. Clin. Microbiol. 28:24372441.
20. Eisenach, K. D.,, M. D. Gave,, J. H. Bates,, and J. T. Crawford. 1990. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J. Infect. Dis. 161: 977981.
21. Eisenach, K. D.,, M. D. Sifibrd,, M. D. Cave,, J. H. Bates,, and J. T. Crawford. 1991. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am. Rev. Respir. Dis. 144:11601163.
22. Evans, K. D.,, A. S. Nakasome,, P. A. Sutherland,, L. M. DeLaMaza,, and E. M. Peterson. 1992. Identification of Mycobacterium tuberculosis and Mycobacterium avium-M. intracellular directly from primary BACTEC cultures by using acridinium ester labeled DNA probes. J. Clin. Microbiol. 30:24272431.
23. French, G. A.,, R. Teoh,, C. Y. Chan,, M. J. Humphries,, S. W. Cheung,, and G. O'Mahoney. 1987. Diagnosis of tuberculous meningitis by detection of tuberculostearic acid in cerebrospinal fluid. Lancet 2:117119.
24. Fries, J. W. U.,, R. J. Patel,, W. F. Piessens,, and D. F. Wirth. 1990. Genus- and species-specific DNA probes to identify mycobacteria using the polymerase chain reaction. Mol. Cell. Probes 4:87105.
25. Grange, J. M. 1984. The humoral immune response in tuberculosis: its nature, biological role, and diagnostic usefulness. Adv. Tuberc. Res. 21:178.
26. Hance, A. J.,, B. Grandchamp,, V. Levy-Frebault,, D. Lecossier,, J. Rauzier,, D. Bocart,, and B. Gicquel. 1989. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol. Microbiol. 3:843849.
27. Hermans, P. W. M.,, A. R. J. Schuitema,, D. van Soolingen,, C. P. H. J. Verstynen,, E. M. Bik,, J. E. R. Thole,, A. H. J. Kolk,, and J. D. A. van Embden. 1990. Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction. J. Clin. Microbiol. 28:12041213.
28. Huebner, R. E.,, R. C. Good,, and J. I. Tokars. 1993a. Current practices in mycobacteriology: results of a survey of state public health laboratories. J. Clin. Microbiol. 31:771775.
29. Huebner, R. E.,, M. F. Schein,, and J. B. Bass, Jr. 1993b. The tuberculin skin test. Clin. Infect. Dis. 17:968975.
30. Ilayashi, K. 1991. PCR-SSCP: a simple and sensitive method for detection of mutations in genomic PCR. PCR Methods Appl. 1:3438.
31. Iovannisci, D. M.,, and E. S. Winn-Deen. 1993. Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA. Mol. Cell. Probes 7:3543.
32. Ivanyi, J., 1991. Serologic tests for the diagnosis of tuberculosis and leprosy, p. 267279. In A. Vaheri,, R. C. Tilton,, and A. Balows (ed.), Rapid Methods and Automation in Microbiology and Immunology. Springer-Verlag, Berlin.
33. Jacobs, W. R.,, R. G. Barletta,, R. Udani,, J. Chan,, G. Kalkut,, G. Sosne,, T. Kieser,, G. J. Sarkis,, G. F. Hatfull,, and B. R. Bloom. 1993. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science 260:819822.
34. Jonas, V.,, M. Alden,, T. Endozo,, P. Hammond,, K. Kamisango,, C. Knott,, R. Lankford,, D. McAllister,, L. Wood,, Y. Yang,, S. McDonough,, and D. Kacian. 1993a. Rapid direct detection of Mycobacterium tuberculosis in respiratory specimens, abstr. U-44, p. 176. Abstr. 93rd Gen. Meet. Am. Soc. Microbiol.
35. Jonas, V.,, M. J. Alden,, J. I. Curry,, K. Kamisango,, C. A. Knott,, R. Lankford,, J. M. Wolfe,, and D. F. Moore. 1993b. Detection and identification of Mycobacterium tuberculosis directly from induced sputum specimens using amplification of rRNA. J. Clin. Microbiol. 31:24102416.
36. Jones, W. D., Jr. 1988. Bacteriophage typing of Mycobacterium tuberculosis cultures from incidents of suspected laboratory cross-contamination. Tubercle 69:4346.
37. Jun Jin, D.,, and C. A. Gross. 1988. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampin resistance. J. Mol. Biol. 202:4558.
38. Kawa, D. E.,, D. R. Pennel,, L. N. Kubista,, and R. F. Schell. 1989. Development of a rapid method for determining the susceptibility of Mycobacterium tuberculosis to isoniazid using the Gen-Probe DNA hybridization system. Antimicrob. Agents Chemother. 33:10001005.
39. Kent, B. D.,, and G. P. Kubica. 1985. Public health mycobacteriology: a guide for the level III laboratory, 207 p. U.S. Department of Health and Human Services. Centers for Disease Control, Atlanta.
40. Koch, R. 1891. Weitere Mittheilung uberdas Tuberkulin. Dtsch. Med. Wochenschr. 17:11891192.
41. Kohne, D. E., 1989. The use of DNA probes to detect and identify microorganisms, p. 1135. In B. Kleger,, D. Jungkind,, E. Hinks,, and L. A. Miller (ed.), Rapid Methods in Clinical Microbiology. Plenum Press, New York.
42. Kolk, A. H. J.,, A. R. J. Schuitema,, S. Kuijper,, J. van Leeuwen,, P. W. M. Hermans,, J. D. A. van Embden,, and R. A. Hartskeerl. 1992. Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and the nonradioactive detection system. J. Clin. Microbiol. 30:25672575.
43. Kramer, F. R.,, S. Tyagi,, C. E. Guerra,, H. Lomeli,, and P. M. Lizardi,. 1991. Q-beta amplification assays, p. 1722. In A. Vaheri,, R. C. Tilton,, and A. Balows (ed.); Rapid Methods and Automation in Microbiology and Immunology. Springer-Verlag, Berlin.
44. Larsson, L.,, P. A. Mardh,, G. Odham,, and G. Westerdahl. 1981. Use of selected ion monitoring for detection of tuberculostearic and C32 mycocerosic acid in mycobacteria and in five-day-old cultures of sputum specimens from patients with pulmonary tuberculosis. Acta Pathol. Microbiol. Scand. Sect. ? 89:245251.
45. Lee, F. K.,, A. J. Nahmias,, S. A. Lowery,, S. E. Reef,, S. E. Thompson,, J. Oleske,, A. Vahline,, and C. Czerkinsky. 1989. ELISPOT—a new approach to the study of the dynamics of virus-immune system interaction for diagnosis and monitoring of HIV infection. AIDS Res. Hum. Retroviruses 5:517523.
46. Lu, Z.,, J. Qiao,, T. Shen,, and H. Link. 1990. Early diagnosis of tuberculosis meningitis by detection of anti-BCG secreting cells in cerebrospinal fluid. Lancet 336:1013.
47. Manjunath, N.,, P. Shankara,, L. Rajan,, A. Bhargava,, S. Saluja, and Shriniwas. 1991. Evaluation of a polymerase chain reaction for the diagnosis of tuberculosis. Tubercle 72:2127.
48. Mardh, P. A.,, L. Larson,, N. Hoiby,, H. C. Engbaek,, and G. Oldham. 1983. Tuberculostearic acid as a diagnostic marker in tuberculous meningitis. Lancet i:367.
49. Miller, N.,, S. G. Hernandez,, and T. Cleary. 1994. Evaluation of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens. J. Clin. Microbiol. 32:393397.
50. Minniken, D. E.,, and M. Goodfellow,. 1980. Lipid composition in the classification and identification of acid-fast bacteria, p. 189256. In M. Goodfellow, and R. G. Board (ed.), Microbiological Classification and Identification. Academic Press, London.
51. Mullis, K. B., and F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335350.
52. National MDR-TB Task Force. 1992. National action plan to combat multidrug-resistant tuberculosis. Morbid. Mortal. Weekly Rep. 41(RR-11):l48.
53. Nesheim, S.,, F. Lee,, M. Sawyer,, D. Jones,, B. Slade,, N. Shaffer,, R. Holmes,, V. Grimes,, M. Rogers,, and A. Nahmias. 1992. Diagnosis of human immunodeficiency virus infection by enzyme-linked immunospot (ELISPOT) assays in a prospectively followed cohort of infants of HIV-seropositive women. Pediatr. Infect. Dis. J. 11:635639.
54. Nolte, F. S.,, B. Metchock,, J. E. McGowan, Jr.,, A. Edwards,, O. Okwumabua,, C. Thurmond,, P. S. Mitchell,, B. Plikaytis,, and T. Shinnick. 1993. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J. Clin. Microbiol. 31:17771782.
55. Nycz, M.,, G. P. Vonk,, J. L. Schram,, G. D. Shank,, and G. T. Walker. Detection of Mycobacterium aviumlintracellular complex specific DNA using strand displacement amplification. Submitted for publication.
56. Palmer, E.,, and L. B. Edwards. 1967. Tuberculin test in retrospect and prospect. Arch. Environ. Health 15:792808.
57. Pao C. C., T. S., Yen,, J. B. You,, J.-S. Maa,, E. H. Fiss,, and C.-H. Chang. 1990. Detection and identification of Mycobacterium tuberculosis by DNA amplification. J. Clin. Microbiol. 28:18771880.
58. Patel, R. J.,, J. W. U. Fries,, W. F. Piessens,, and D. F. Wirth. 1990. Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment of Mycobacterium tuberculosis. J. Clin. Microbiol. 28:513518.
59. Pfyffer, G. E.,, P. Kissling,, R. Wirth,, and R. Weber. 1994. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system. J. Clin. Microbiol. 32:918923.
60. Plikaytis, B. B., R. H. Gelber,, and T. M. Shinnick. 1990. Rapid and sensitive detection of Mycobacterium leprae using a nested primer gene amplification assay. J. Clin. Microbiol. 28:19131917.
61. Plikaytis, B. B., B. D. Plikaytis,, M. A. Yakrus,, W. R. Butler,, C. L. Woodley,, V. A. Silcox,, and T. M. Shinnick. 1992. Differentiation of slow growing Mycobacterium species including Mycobacterium tuberculosis by gene amplification and restriction fragment length polymorphism. J. Clin. Microbiol. 30:18151822.
62. Rogall, T.,, T. Flohr,, and E. C. Böttger. 1990. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J. Gen. Microbiol. 136:19151920.
63. Savic, B., U. Sobring,, S. Alugupalli,, L. Larsson,, and H. Miörner. 1992. Evaluation of polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum. J. infect. Dis. 166:11771180.
64. Schram, J. L.,, D. D. Shank,, and G. T. Walker. DNA probe detection of Mycobacterium avium and Mycobacterium intracellular genomic DNA using strand displacement amplification (SDA). Submitted for publication.
65. Sedgewick, J. D.,, and P. G. Holt. 1983. A solid phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J. Immunol. Methods 65:301309.
66. Shawar, R. M.,, F. A. K. El Zaatari,, A. Nataraj,, and J. E. Clarridge. 1993. Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and honisotopic hybridization methods. J. Clin. Microbiol. 31:6165.
67. Sjobring, U.,, M. Mecklenburg,, A. ?. Andersen, and H. Miörner. 1990. Polymerase chain reaction for detection of Mycobacterium tuberculosis. J. Clin. Microbiol. 28:22002204.
68. Smithwick, R. W. 1976. Laboratory Manual for Acid-Fast Microscopy, 2nd ed. Center for Disease Control, Atlanta.
69. Soini, H.,, M. Skurnik,, K. Liippo,, E. Tala,, and M. K. VUjanen. 1992. Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kDa protein. J. Clin. Microbiol. 30:20252028.
70. Spargo, C. A.,, P. D. Haaland,, S. R. Jurgensen,, D. D. Shank,, and G. T. Walker. 1993.Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex. Mol. Cell. Probes 7:395404.
71. Sritharan, V.,, and R. H. Barker. 1991. A simple method for diagnosing Mycobacterium tuberculosis infections in clinical samples using PCR. Mol. Cell. Probes 5:385395.
72. Stead, W. W.,, T. To,, R. W. Harrison,, and J. H. Abraham. 1987. Benefit-risk consideration in preventive treatment for tuberculosis in elderly persons. Ann. Intern. Med. 107:834845.
73. Telenti, A.,, P. Imboden,, F. Marchesi,, D. Lowrie,, S. Cole,, M. J. Colston,, L. Matter,, K. Schopfer,, and T. Bodmer. 1993. Detection of rifampin-resistance mutations in Mycobacterium tuberculosis. Lancet 341: 647650.
74. Urdea, M. S., 1991. Controlled synthetic oligonucleotide networks for the detection of pathogenic organisms, p. 15. In A. Vaheri,, R. C. Tilton,, and A. Balows (ed.), Rapid Methods and Automation in Microbiology and Immunology. Springer-Verlag, Berlin.
75. Victor, T.,, R. DuToit,, and P. D. VanHeiden. 1992. Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction. J. Clin. Microbiol. 30: 15141517.
76. Walker, G. T.,, M. S. Fraiser,, J. L. Schram,, M. C. Little,, J. D. Nadeau,, and D. P. Malinowski. 1992a. Strand displacement amplification—an isothermal in vitro DNA amplification technique. Nucleic Acids Res. 20:16911696.
77. Walker, G. T.,, M. C. Little,, J. D. Nadeau,, and D. D. Shank. 1992b. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc. Natl. Acad. Sci. USA 89:392396.
78. Walker, G. T.,, J. D. Nadeau,, P. A. Spears,, J. L. Schram,, M. Nycz,, and D. D. Shank. A DNA probe test for M. tuberculosis and other mycobacterial pathogens. Submitted for publication.
79. Zhang, J.,, B. Heym,, B. Allen,, D. Young,, and S. Cole. 1992. The catalase-peroxidase gene of Mycobacterium tuberculosis. Nature (London) 358:591593.

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