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Chapter 6 : Cultivation of for Research Purposes

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Abstract:

Individual investigators have different reasons for cultivating in their laboratories; the medium to be used and the conditions of incubation should be tailored to the specific goals, taking into account the physiologic characteristics of this organism. A classic description of , which would be only partially accurate, might characterize this organism as a fastidious, slowly growing, strictly aerobic, lipid-rich, hydrophobic, acid-fast bacterial rod. Much of the early research on the tubercle bacillus was directed toward production of tuberculin or other crude chemical products of the bacilli, such as unique lipids or polysaccharides. The growth of tubercle bacilli in the synthetic media cited is difficult to follow in quantitative terms, as it cannot be measured optically and since simple plating is not accurate because of the severe clumping of the bacilli. Media based on a Tween 80-albumin formulation are satisfactory for cultivation of tubercle bacilli for studies of growth rates and kinetics and, in most cases, for preparation of bacilli for chemical extraction. Glycerol has commonly been used in media when large crops of bacilli are desired, since this carbohydrate markedly stimulates growth of (note that glycerol inhibits growth of fresh isolates of the closely related ). is known to produce enzymes of anaerobic metabolism, and these are especially prominent when bacilli are tested after direct harvest from host tissues; there is a general shift away from O-dependent pathways to anaerobic or facultative anaerobic pathways in host derived bacilli compared to cultured organisms.

Citation: Wayne L. 1994. Cultivation of for Research Purposes, p 73-83. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch6
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Figures

Image of Figure 1.
Figure 1.

Eight-day growth of in Tween-albumin broth incubated in a gradient temperature block. Growth is expressed as optical absorbance (A). Triplicate tubes were incubated at each temperature, and the results were plotted as the mean A, with vertical bars spanning ±1 standard deviation.

Citation: Wayne L. 1994. Cultivation of for Research Purposes, p 73-83. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch6
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References

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1. Affronti, L. F.,, V. Porrello,, and S. Gupta. 1990. Trace elements incorporated into the culture medium of Mycobacterium tuberculosis promote the presence of tuberculoprotein C in the preparation of purified protein derivatives. Microbios 63: 101 107.
2. Baisden, L. A. 1951. Trace element supplements for asparagine brands found to be deficient for the growth of tubercle bacilli. Am. J. Vet. Res. 12: 254 256.
3. Barrow, W. W.,, B. P. Ullom,, and P. J. Brennan. 1980. Peptidoglycolipid nature of the superficial cell wall sheath of smooth-colony-forming mycobacteria. J. Bacterial. 144: 814 822.
4. Behling, C. A.,, B. Bennett,, K. Takayama,, and R. L. Hunter. 1993. Development of a trehalose 6,6'-dimycolate model which explains cord formation by Mycobacterium tuberculosis. Infect. Immun. 61: 2296 2303.
5. Bowles, J. A.,, and W. Segal. 1965. Kinetics of utilization of organic compounds in the growth of Mycobacterium tuberculosis. J. Bacterial. 90: 157 163.
6. Collins, F. M.,, L. G. Wayne,, and V. Montalbine. 1974. The effect of cultural conditions on the distribution of Mycobacterium tuberculosis in the spleens and lungs of specific pathogen-free mice. Am. Rev. Respir. Dis. 110: 147 156.
7. Davis, B. D.,, and R. J. Dubos. 1948. The inhibitory effect of lipase on bacterial growth in media containing fatty acid esters. J. Bacteriol. 55: 11 23.
8. Dubos, R. J. 1945. Rapid and submerged growth of mycobacteria in liquid media. Proc. Soc. Exp. Biol. Med. 58: 361 362.
9. Dubos, R. J. 1950. The effect of organic acids on mammalian tubercle bacilli. J. Exp. Med. 92: 319 332.
10. Dubos, R. J.,, and B. D. Davis. 1946. Factors affecting the growth of tubercle bacilli in liquid media . J. Exp. Med. 83: 409 423.
11. Dubos, R. J.,, and G. Middlebrook. 1947. Media for tubercle bacilli. Am. Rev. Tuberc. 56: 334 345.
12. Dubos, R. J.,, and G. Middlebrook. 1948. The effect of wetting agents on the growth of tubercle bacilli. J. Exp. Med. 88: 81 88.
13. Fisher, M. W.,, and W. Kirchheimer. 1952. Studies on the growth of mycobacteria. I. The occurrence of arithmetic linear growth. Am. Rev. Tuberc. 66: 758 761.
14. Gheorghiu, M.,, P. H. Lagrange,, and C. Fillastre. 1988. The stability and immunogenicity of a dispersed-grown freeze-dried Pasteur BCG vaccine. J. Biol. Stand. 16: 15 26.
15. Hill, E. G.,, L. G. Wayne,, and W. M. Gross. 1972. Purification of mycobacterial deoxyribonucleic acid. J. Bacteriol. 112: 1033 1039.
16. Krasnow, I.,, and L. G. Wayne. 1969. Comparison of methods for tuberculosis bacteriology. Appl. Microbiol. 18: 915 917.
17. Kubica, G. P.,, and L. G. Wayne. 1984. The Mycobacteria—a Sourcebook. Marcel Dekker, Inc., New York.
18. Lefford, M. J., 1984. Diseases in mice and rats, p. 947 977. In G. P. Kubica, and L. G. Wayne (ed.), The Mycobacteria: a Source Book. Marcel Dekker, Inc., New York.
19. Lyon, R. H.,, H. C. Lichstein,, and W. H. Hall. 1961. Factors affecting the growth of Mycobacterium tuberculosis in aerobic and shake culture. Am. Rev. Respir. Dis. 83: 255 260.
20. Mackaness, G. B.,, D. J. Auclair,, and P. H. Lagrange. 1973. Immuno-potentiation with BCG: immune response to different strains and preparations.J. Natl. Cancer Inst. 51: 1655 1667.
21. Roberts, G. D.,, E. W. Koneman,, and Y. K. Kim,. 1991. Mycobacterium, p. 304 339. In A. Balows,, W. J. Hausler Jr.,, K. L. Herrmann,, H. D. Isenberg,, and H. J. Shadomy (ed.), Manual of Clinical Microbiology, 5th ed. American Society for Microbiology, Washington, D.C.
22. Segal, W., 1984. Growth dynamics of in vivo and in vitro grown mycobacterial pathogens, p. 547 573. In G. P. Kubica, and L. G. Wayne (ed.), The Mycobacteria—a Sourcebook. Marcel Dekker, Inc., New York.
23. Soltys, M. A. 1952. Tubercle Bacillus and Laboratory Methods in Tuberculosis. E. & S. Livingstone Ltd., Edinburgh.
24. Tepper, B. S. 1968. Differences in the utilization of glycerol and glucose by Mycobacterium phlei. J. Bacteriol. 95: 1713 1717.
25. Turcotte, R. 1969a. Yield of non-dialyzable mycobacterial constituents during the growth cycle. Can. J. Microbiol. 15: 35 41.
26. Turcotte, R. 1969b. The variations in the antigenic composition of Mycobacterium tuberculosis during the growth cycle as measured by the passive hemagglutination and precipitation reactions. Can. J. Microbiol. 15: 681 687.
27. Volk, W. A.,, and Q. N. Myrvik. 1953. An explanation for the arithmetic linear growth of mycobacteria. J. Bacteriol. 66: 386 388.
28. Wayne, L. G. 1962. Preparation of suspensions of tubercle bacilli on a Vortex mixer. Am. J. Clin. Pathol. 37: 328 329.
29. Wayne, L. G. 1970. Temperature adaptations and biochemical activities of group III mycobacteria. Pneumonology 142: 278 283.
30. Wayne, L. G. 1976. Dynamics of submerged growth of Mycobacterium tuberculosis under aerobic and microaerophilic conditions. Am. Rev. Respir. Dis. 114: 807 811.
31. Wayne, L. G. 1977. Synchronized replication of Mycobacterium tuberculosis. Infect. Immun. 17: 528 530.
32. Wayne, L. G.,, and G. A. Diaz. 1967. Autolysis and secondary growth of Mycobacterium tuberculosis in submerged culture. J. Bacteriol. 93: 1374 1381.
33. Wayne, L. G.,, and G. P. Kubica,. 1986. Section 16. Mycobacteria, p. 1436 1457. In P. H. A. Sneath,, N. S. Mair,, M. E. Sharpe,, and J. G. Holt (ed.), Bergey's Manual of Systematic Bacteriology, vol. 2. The Williams & Wilkins Co., Baltimore.
34. Wayne, L. G.,, and K.-Y. Lin. 1982. Glyoxylate metabolism and adaptation of Mycobacterium tuberculosis to survival under anaerobic conditions. Infect. Immun. 37: 1042 1049.
35. Wayne, L. G.,, and H. A. Sramek. 1979. Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis. Infect. Immun. 24: 363 370.
36. Youmans, G. P. 1979. Antigenic The W.B. Saunders Co., Philadelphia.

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