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Chapter 7 : Current Laboratory Methods for the Diagnosis of Tuberculosis

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Abstract:

This chapter presents a broad picture of activities in a diagnostic mycobacteriology laboratory. It addresses the methodology currently available in most laboratories as well as the techniques that require further standardization and therefore have been implemented in only a few laboratories, mostly in conjunction with research and development. It provides short descriptions of these new methods in order to review their possible application in clinical laboratories. It also gives a condensed description of biosafety practices. In order to present a comprehensive review of all activities that take place in a clinical mycobacteriology laboratory, the chapter focuses on biosafety in the mycobacteriology laboratory, and specimen collection, smear examination, and inoculation of primary culture media. It then describes methods for identification of . Finally, the chapter talks about drug susceptibility testing for detection of initial and acquired drug resistance.

Citation: Heifets L, Good R. 1994. Current Laboratory Methods for the Diagnosis of Tuberculosis, p 85-110. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch7
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Figure 1

M. tuberculosis in a smear from a concentrated sputum specimen. Photography by N. Mor. (A and B) Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, ×1,125. (C) Auramine O stain; fluorescence microscope, Olympus model BH2 with 40x objective. Total magnification, × 1, 440. (D) Auramine O stain; fluorescence microscope, model Leitz-Wetzlar with 40x objective. Total magnification, × 2, 000.

Citation: Heifets L, Good R. 1994. Current Laboratory Methods for the Diagnosis of Tuberculosis, p 85-110. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch7
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Image of Figure 2
Figure 2

M. tuberculosis in cultures. Ziehl-Nielsen stain; Olympus microscope model BH2 with l00x objective. Total magnification, × 1,125. Photography by N. Mor. (A) Smear from a culture grown on 7H11 agar; (B) "cord" formation in 7H12 broth; (C and D) intracellular growth in human-monocyte-derived macrophages.

Citation: Heifets L, Good R. 1994. Current Laboratory Methods for the Diagnosis of Tuberculosis, p 85-110. In Bloom B (ed), Tuberculosis. ASM Press, Washington, DC. doi: 10.1128/9781555818357.ch7
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