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Chapter 17 : Regulation of Phosphorus Metabolism

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Abstract:

This chapter focuses on the genes that are expressed in response to phosphate starvation, i.e., genes of the phosphate stimulon. Special emphasis is given to genes of the PHO regulon, which are dependent on the PhoP-PhoR two-component regulators. The study of the regulation of phosphate metabolism in species in general and in specifically has been complicated by a feature that increases the potential importance of this process: alkaline phosphatases (APases) are encoded by multiple structural genes. Secreted APases and APases from different cell fractions have similar physical and chemical properties. Genes regulated by the PHO regulon show overlapping regulation with other global regulatory systems outside of the phosphate stimulon. All genes that are known to require PhoP and PhoR for expression are also controlled b y SpoOA. This is consistent with the thought that phosphorylated SpoOA controls genes needed for various stationary-phase phenomena and not only those needed for sporulation, since phosphate-starved cultures enter postexponential growth because of limited phosphate availability. Further analysis of promoters controlled by phosphate starvation and a two-component system other than PhoPPhoR, such as gsiA, which requires ComP-ComA, may reveal additional network circuitry between the two component systems.

Citation: Hulett F. 1993. Regulation of Phosphorus Metabolism, p 229-235. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch17

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Figure 1

Genetic map of phosphate stimulon genes in . Gene loci indicated on the inside of the circle are for reference and include all the loci used in mapping Tn917 insertions ( ). The psi promoters (on the outside of the circle) show phosphate starvation-inducible expression; they were identified by screening a Tn fusion library. was given a pho designation because the Tn interruption was in the structural gene for APaseB. identifies the phoP-phoR operon regulatory locus. identifies the mapping location of the gene encoding APaseA.

Citation: Hulett F. 1993. Regulation of Phosphorus Metabolism, p 229-235. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch17
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References

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Tables

Generic image for table
Table 1

Classification of phosphate starvation-inducible promoters based on their PhoP-PhoR dependencies ( )

Citation: Hulett F. 1993. Regulation of Phosphorus Metabolism, p 229-235. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch17

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