Chapter 21 : Biosynthesis of Arginine, Proline, and Related Compounds

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Biosynthesis of Arginine, Proline, and Related Compounds, Page 1 of 2

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General aspects of arginine and proline metabolism in prokaryotes have been covered in various reviews. But studies with gram-positive bacteria are regrettably fragmentary. and probably other species of catabolize arginine via 4-guanidinobutyramide and 4-guanidinobutyrate. It has been reported that possesses two P5C dehydrogenases, whose synthesis is controlled by arginine or proline. The cyclodeaminase reaction, which yields proline from ornithine in species, seems to function primarily for ornithine catabolism rather than proline biosynthesis. The -- clusters from and were first cloned in by selecting for complementation of arginine auxotrophs. A significant finding was that where as mutations in , , or have no effect on levels of the biosynthetic enzymes, mutations lead to simultaneous loss of repressibility of the biosynthetic enzymes and of inducibility of the catabolic enzymes, with the implication that these two controls share at least one common component. Mutations to proline auxotrophy map at a further locus, ; these mutants do not respond to arginine, possibly because in spp., levels of arginase are low and show only weak inducibility by arginine. Arginine, like many aminoacids, is a precursor of many of the secondary metabolites (including antibiotics) elaborated by spp. An intriguing connection between proline catabolism and transport and the production of a secondary metabolite, undecylprodigiosin, of which proline is a precursor, was found in .

Citation: Baumberg S, Klingel U. 1993. Biosynthesis of Arginine, Proline, and Related Compounds, p 299-306. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch21

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Acetyl Coenzyme A
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Figure 1

Pathways of arginine, proline, and polyamine metabolism in and =, pathways found in both organisms; —, pathways found only in Numbered enzymes mediating the various steps are listed in Table 1 . The following pathways are referred to in this chapter but not indicated in this figure: the ornithine cyclase pathway of proline biosynthesis and catabolism spp.) and the guanidinobutyrate pathway of arginine catabolism spp.).

Citation: Baumberg S, Klingel U. 1993. Biosynthesis of Arginine, Proline, and Related Compounds, p 299-306. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch21
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Generic image for table
Table 1

Enzyme reactions of arginine, proline, and polyamine metabolism as numbered in Fig. 1

Citation: Baumberg S, Klingel U. 1993. Biosynthesis of Arginine, Proline, and Related Compounds, p 299-306. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch21

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