Chapter 49 : Protein Secretion

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The basic mechanism by which proteins are transported across membranes appears to be universal, with important features conserved between bacteria and eukaryotes. Protein secretion across the cell envelope is a complex process. Protein transport involves an interaction between the exported protein and the cellular secretion factors. Some of the characteristics of protein secretion in and are similar. The first part of this chapter discusses some of the structural features of the exoproteins and their role in protein secretion. This discussion is followed by an outline of the approaches taken to define the components of secretion machinery and of one's current knowledge of protein secretion in . In this discussion, the term “translocation” refers to the transfer of protein across the membrane. The signal sequence mutants described were constructed to improve the secretion efficiency of the wheat α-amylase signal sequence in . The wheat α--amylase signal peptide can transport LVS and alkaline phosphatase from , but the kinetics of transport were slower than those observed with signal peptides. This observation underlines an important role played by the mature sequence in protein secretion. The difference in phenotype between and was useful in cloning the leader peptidase gene. These two complementary approaches have identified mutations within the same structural genes, underlining the importance of these genes in protein export.

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49

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Bacterial Proteins
Outer Membrane Proteins
Integral Membrane Proteins
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Generic image for table
Table 1

Signal peptide sequences

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49
Generic image for table
Table 2

Lipoprotein signal sequences

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49

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