Chapter 49 : Protein Secretion

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The basic mechanism by which proteins are transported across membranes appears to be universal, with important features conserved between bacteria and eukaryotes. Protein secretion across the cell envelope is a complex process. Protein transport involves an interaction between the exported protein and the cellular secretion factors. Some of the characteristics of protein secretion in and are similar. The first part of this chapter discusses some of the structural features of the exoproteins and their role in protein secretion. This discussion is followed by an outline of the approaches taken to define the components of secretion machinery and of one's current knowledge of protein secretion in . In this discussion, the term “translocation” refers to the transfer of protein across the membrane. The signal sequence mutants described were constructed to improve the secretion efficiency of the wheat α-amylase signal sequence in . The wheat α--amylase signal peptide can transport LVS and alkaline phosphatase from , but the kinetics of transport were slower than those observed with signal peptides. This observation underlines an important role played by the mature sequence in protein secretion. The difference in phenotype between and was useful in cloning the leader peptidase gene. These two complementary approaches have identified mutations within the same structural genes, underlining the importance of these genes in protein export.

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49

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Bacterial Proteins
Outer Membrane Proteins
Integral Membrane Proteins
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1. Banerjee, S.,, and J. N. Hansen. 1988. Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic. J. Biol. Chem. 263:95089514.
2. Behnke, D.,, and D. Gerlach. 1987. Cloning and expression in Escherichia coli, Bacillus subtilis, and Streptococcus sanguis of a gene for staphylokinase—a bacterial plasminogen activator. Mol. Gen. Genet. 210:528534.
3. Benyahia, F.,, R. Chambert,, and M. F. Petlt-Glatron. 1988. Levansucrase of Bacillus subtilis: effects on the secretion process of single amino acid substitutions in the mature part of the protein. J. Gen. Microbiol. 134: 32593268.
4. Borchert, T. V. 1991. A genetic approach in the study of protein secretion in B. subtilis. Ph.D. thesis. The Technical University of Denmark, Copenhagen.
5. Borchert, T. V.,, and V. Nagarajan,. 1990. Structure-function studies on the Bacillus amyloliquefaciens levansucrase signal peptide, p. 171177. In M. Zukowski,, A. T. Ganesan,, and J. Hoch (ed.), Genetics and Biotechnology of Bacilli, Academic Press, Inc., New York.
6. Borchert, T. V.,, and V. Nagarajan. 1991. Effect of signal sequence alteration on export of levansucrase in Bacillus subtilis. J. Bacteriol. 173:276282.
7. Briggs, M. S. and L. M. Gierasch. 1986. Molecular mechanisms of protein secretion: the role of the signal sequence. Adv. Prot. Chem. 38:110180.
8. 7a.Buchanan, C. E., and M. Ling. 1992. Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis. J. Bacteriol. 174:17171725.
8.Buchanan, C. E., and S. L. Neyman. 1986. Correlation of penicillin-binding protein composition with different functions of two membranes in Bacillus subtilis fore-spores. J. Bacteriol. 165:498503.
9. Caulfield, M. P.,, R. C. W. Berkeley,, E. A. Pepper,, and J. Melllng. 1979. Export of extracellular levansucrase by Bacillus subtilis: inhibition by cerulenin and quinacrine. J. Bacteriol. 138:345351.
10. Chambert, R.,, F. Benyahia,, and M. F. Petit-Glatron. 1990. Secretion of Bacillus subtilis levansucrase Fe(III) could act as a cofactor in efficient coupling of folding and translocation processes. Biochem. J. 265:375382.
11. Chambert, R., andM. F. Petit-Glatron. 1990. Reversible thermal unfolding of Bacillus subtilis levansucrase is modulated by Fe3+ and Ca2+. FEBS Lett. 275:6164.
11a. Chen, M.,, and V. Nagarajan. The role of signal peptide and mature protein in RNase (Barnase) export from Bacillus subtilis. Mol. Gen. Genet., in press.
12. Cheung, H. Y.,, and E. Freese. 1985. Monovalent cations enable cell wall turnover of the turnover-deficient mutant of Bacillus subtilis. J. Bacteriol. 161:12221225.
13. Chung, Y. J.,, M. T. Steen,, and J. N. Hansen. The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport mechanism. Submitted for publication.
14. Doyle, R., 1989. How cell walls of gram-positive bacteria interact with metal ions, p. 275293. In T. J. Beverage, and R. J. Doyle (ed.), Metal Ions in the Bacteria. John Wiley & Sons, Inc., New York.
15. Egnell, P.,, and J.-I. Flock. 1991. The subtilisin Carlsberg pro-region is a membrane anchorage for two fusion proteins produced in Bacillus subtilis. Gene 97: 4954.
16. Emr, S. D.,, S. Hanley-Way,, and T. J. Silhavy. 1981. Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 23:7988.
17. Fandl, J.,, and P. C. Tal. 1990. Biochemical characterization of genetically defined translocation components. J. Bioenerg. Biomembr. 22:369388.
18. Flshman, Y.,, S. Rottem,, and N. Citri. 1980. Preferential suppression of normal exoenzyme formation by membrane-modifying agents. J. Bacteriol. 141:14351438.
19. Fukusaki, E.,, W. Panbangred,, A. Shinmyo,, and H. Okada. 1984. The complete nucleotide sequence of the xylanase gene (xynA) of Bacillus pumilus. FEBS Lett. 171:197201.
20. Gennity, J.,, J. Goldstein,, and M. Inouye. 1990. Signal peptide mutants of Escherichia coli. J. Bioenerg. Biomembr. 22:233270.
21. Gierasch, L. M. 1989. Signal sequences. Biochemistry 28:923930.
22. Gould, A. R.,, B. K. May,, and W. H. Elliott. 1975. Release of extracellular enzymes from Bacillus amyloliquefaciens. J. Bacteriol. 122:3440.
23. Hansen, J. N., 1992. The molecular biology of nisin and its structural analogs. In D. Hoover, and L. Steenson (ed.), Bacteriocins of Lactic Acid Bacteria, in press. Academic Press, Inc., New York.
24. Hartley, R. W. 1988. Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. J. Mol. Biol. 202:913915.
25. Hastrup, S.,, and M. F. Jacobs,. 1990. Lethal phenotype conferred by xylose-induced overproduction of an Apr-lacZ fusion protein, p. 3341. In M. Zukowski,, A. T. Ganesan,, and J. Hoch (ed.), Genetics and Biotechnology of Bacilli, vol. 3. Academic Press, Inc., New York.
26. Hayashi, S.,, S. Y. Chang,, S. Chang, C. Z. Giam, and H. C. Wu. 1985. Modification and processing of internalized signal sequences of prolipoprotein in Escherichia coli and in Bacillus subtilis. J. Biol. Chem. 260: 57535759.
27. Hayashi, S.,, and H. C. Wu. 1990. Lipoproteins in bacteria. J. Bioenerg. Biomembr. 22:451472.
28. Hemila, H. 1991. Sequence of a PAL related lipoprotein from Bacillus subtilis. FEMS Microbiol. Lett. 82:3742.
29. Hofemeister, J.,, A. Kurtz,, R. Borriss,, and J. Knowles. 1986. The β-glucanase gene from Bacillus amylolique-faciens shows extensive homology with that of Bacillus subtilis. Gene 49:177187.
30. Horiuchi, S.,, D. Marty-Mazars,, P. C. Tal,, and B. D. Davis. 1983. Localization and quantitation of proteins characteristic of the complexed membrane of Bacillus subtilis. J. Bacteriol. 154:12151221.
31. Horiuchi, S.,, P. C. Tai,, and B. D. Davis. 1983. A 64-kilodalton membrane protein of Bacillus subtilis covered by secreting ribosomes. Proc. Natl. Acad. Sci. USA 80:32873291.
32. Hulett, F. M.,, E. E. Kim, C. Bookstein, N. V. Kapp, C. W. Edwards, and H. W. Wyckoff. 1991. Bacillus subtilis alkaline phosphatases III and IV. J. Biol. Chem. 172:10771084.
33. Hulett, F. M.,, K. Stuckmann,, D. Spencer,, and T. San-opoulou. 1986. Purification and characterization of the secreted alkaline phosphatase of Bacillus licheniformis MCI4: identification of a possible precursor. J. Gen. Microbiol. 132:23872395.
34. Hussaln, M.,, F. I. J. Pastor,, and J. O. Lampen. 1987. Cloning and sequencing of the blaZ gene encoding β-lactamase III. A lipoprotein of Bacillus cereus 569/H. J. Bacteriol. 169:579586.
35. Iino, T.,, M. Takahashi,, and T. Sako. 1987. Role of amino-terminal positive charge on signal peptide in staphylokinase export across the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 262:74127417.
36. Ikemura, H.,, and M. Inouye. 1988. In vitro processing of pro-subtilisin produced in Escherichia coli. J. Biol. Chem. 263:1295912963.
37. Ikemura, H.,, H. Takagis,, and M. Inouye. 1987. Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J. Biol. Chem. 262:78597864.
38.Ito, K. 1990. Structure, function and biogenesis of SecY, an integral membrane protein involved in protein export. J. Bioenerg. Biomembr. 22:353368.
39. Jacobs, M.,, M. Ellasson,, M. Uhlen,, and J.-I. Flock. 1985. Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res. 13:89138926.
40. Jones, J. D., C. J. Macknlght, and L. Gierasch. 1990. Biophysical studies of signal peptides: implications for signal sequence functions and involvement of lipid in protein export. J. Bioenerg. Biomembr. 22:213232.
41. Kennedy, M. B., and W. J. Lennarz. 1979. Characterization of the extracellular lipase of Bacillus subtilis and its relationship to a membrane-bound lipase found in a mutant strain. J. Biol. Chem. 254:10801089.
41a. Klose, M.,, K. Schimz,, J. van der Wolk,, A. Driessen,, and R. Freudl. Lysine106 of the putative catalytic ATP-binding site of the B. subtilis SecA protein is required for functional complementation of Escherichia coli secA mutants in vivo. Submitted for publication.
42. Kontinen, V.,, P. Saris,, and M. Sarvas. 1991. A gene (prsA) of Bacillus subtilis involved in a novel, late stage of protein export. Mol. Microbiol. 5:12731283.
43. Kontinen, V.,, and M. Sarvas. 1988. Mutants of B. subtilis defective in protein export. J. Gen. Microbiol. 134:23332344.
44. Kovacevic, S.,, L. E. Veal,, H. M. Hsiung,, and J. R. Miller. 1985. Secretion of staphylococcal nuclease by Bacillus subtilis. J. Bacteriol. 162:521528.
45. Kubo, M.,, and T. Imanaka. 1988. Cloning and nucleotide sequence of the highly thermostable neutral protease gene from Bacillus stearothermophilus. J. Gen. Microbiol. 134:18831892.
46. Lampen, J. O.,, W. Wang,, P. S. F. Mezes,, and Y. Yang,. 1984. β-Lactamases of bacilli: nature and processing, p. 129140. In A. T. Ganesan, and J. Hoch (ed.), Genetics and Biotechnology of Bacilli. Academic Press, Inc., New York.
47. Li, M.,, and S. L. Wong. 1992. Cloning and characterization of groESL operon from Bacillus subtilis. J. Bacteriol. 174:39813992.
48. Liu, G.,, T. B. Topping,, and L. L. Randall. 1989. Physiological role during export for the retardation of folding by the leader peptide of maltose-binding protein. Proc. Natl. Acad. Sci. USA 86:92139217.
49. Liu, W.,, and J. N. Hansen. 1991. Conversion of Bacillus subtilis 168 to a subtilin producer by competence transformation. I. Bacteriol. 173:73877390.
50. Lofdahl, S.,, B. Guss,, M. Uhlen,, L. Philpson,, and M. Lindenberg. 1983. Gene for staphylococcal protein A. Proc. Natl. Acad. Sci. USA 80:697701.
51. MacKay, R. M.,, A. Lo,, G. Willick,, M. Zuker,, S. Baird,, M. Dove,, F. Moranelli, and Verner Seligy. 1986. Structure of a Bacillus subtilis endo-β-l,4-glucanase gene. Nucleic Acids Res. 14:91599170.
52.Manoil, C, and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82:81298133.
53. Martin, I.,, M. Débarbouille,, E. Ferrari,, A. Klier,, and G. Rapoport. 1987. Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase. Mol. Gen. Genet. 208:177184.
54. Marty-Mazars, D.,, S. Horiuchi,, P. C. Tai,, and B. D. Davis. 1983. Proteins of ribosome-bearing and free-membrane domains in Bacillus subtilis. J. Bacteriol. 154:13811388.
55. Matsuyama, S.,, E. Kimura,, and S. Mizushlma. 1990. Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allow ATP binding to its amino-terminal region. J. Biol. Chem. 265:87608765.
56. McLaughlin, J. R., C. L. Murray, and J. C. Rabinowitz. 1981. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus β-lactamase gene. J. Biol. Chem. 256:1128311291.
57. Mezes, P. S. F.,, R. H. Blacher,, and J. O. Lampen. 1985. Processing of Bacillus cereus 569/Hβ-lactamaseI in Escherichia coli and Bacillus subtilis. J. Biol. Chem. 260:12181223.
58. Mezes, P. S. F.,, W. Wang,, E. C. H. Yeh,, and J. O. Lampen. 1983. Construction of penP-1, Bacillus licheniformis 749/Cβ-lactamaselacking site for lipoprotein modification. J. Biol. Chem. 258:1121111218.
59. Michealls, S.,, H. Inouye,, D. Oliver,, and J. Beckwith. 1983. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli. J. Bacteriol. 154: 366374.
60. Muren, E. M.,, and L. Randall. 1985. Export of α-amylase by Bacillus amyloliquefaciens requires proton motive force. J. Bacteriol. 164:712716.
61. Murphy, N.,, D. J. McConnell,, and B. A. Cantwell. 1984. The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme b-glucanase. Nucleic Acids Res. 12:53555367.
61a. Nagarajan, V. Unpublished data.
62. Nagarajan, V.,, H. Albertson,, M. Chen,, and J. Ribbe. 1992. Modular vectors for protein expression in Bacillus subtilis. Gene 114:121126.
63. Nagarajan, V.,, and T. V. Borchert. 1991. Levansucrase—a tool to study protein secretion in Bacillus subtilis. Res. Microbiol. 142:787792.
64. Nagarajan, V.,, and M. Chen. Unpublished data.
65. Nakajima, R.,, T. Imanaka,, and S. Alba. 1985. Nucleotide sequence of the Bacillus stearothermophilus α-amylase Gene. J. Bacteriol. 163:401406.
66. Nakamura, A.,, T. Uozumi,, and T. Beppu. 1987. Nucle-otide sequence of a cellulase gene of Bacillus subtilis. Eur.J. Biochem. 164:317320.
67. Nakamura, K.,, Y. Fujita,, Y. Itoh,, and K. Yamane. 1989. Modification of length, hydrophobic properties and electric charge of Bacillus subtilis a amylase signal peptide and their effects on the production of secretory proteins in B. subtilis and Escherichia coli cells. Mol. Gen. Genet. 216:19.
68. Nakamura, K.,, A. Nakamura,, H. Takamatsu,, H. Yoshi-kawa,, and K. Yamane. 1990. Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY. J. Biochem. 107:603607.
69. Nakamura, K.,, H. Takamatsu,, Y. Akiyama,, K. Ito,, and K. Yamane. 1990. Complementation of the protein transport defect of an Escherichia coli secY mutant (secY24) by Bacillus subtilis secY homologue. FEBS Lett. 273:7578.
70. Neugebauer, K.,, R. Sprengel,, and H. Schaller. 1981. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium. Nucleic Acids Res. 9:25772588.
71. Nielsen, J. B. K.,, M. P. Caufield,, and J. O. Lampen. 1981. Lipoprotein nature of Bacillus licheniformis membrane penicillinase. Proc. Natl. Acad. Set. USA 78:35113515.
72. Nielsen, J. B. K.,, and J. O. Lampen. 1982. Membrane-bound penicillinase in gram-positive bacteria. J. Biol. Chem. 257:44904495.
73. Nielsen, J. B. K.,, and J. O. Lampen. 1982. Glyceride-cysteine lipoproteins and secretion by gram-positive bacteria. J. Bacteriol. 152:315322.
74. Nishiya, Y.,, and T. Imanaka. 1990. Cloning and nucleotide sequences of the Bacillus stearothermophilus neutral protease gene and its transcriptional activator gene. J. Bacteriol. 172:48614869.
75. Ohmura, K.,, K. Nakamura,, H. Yamazaki,, T. Shiroza,, K. Yamane,, Y. Jigami,, H. Tanaka,, K. Yoda,, M. Ya-masaki,, and G. Tamura. 1984. Length and structural effect of signal peptides derived from Bacillus subtilis α-amylase on secretion of Escherichia coli β-lactamase in B. subtilis cells. Nucleic Acids Res. 12:53075319.
76. Oliver, D. B., R. J. Cabelll, and G. P. Jarosik. 1990. SecA protein: autoregulated initiator of secretory precursor protein translocation across the E. coli plasma membrane. J. Bioenerg. Biomembr. 22:311336.
77.Overhoff, B., M. Klein, M. Spies, and R. Freudl. 1991. Identification of a gene fragment which codes for the 364 amino terminal amino acid residues of a SecA homologue from Bacillus subtilis: further evidence for the conservation of the protein export apparatus in gram-positive and gram-negative bacteria. Mol. Gen. Genet. 228:417423.
78.Paddon, C. J., and R. W. Hartley. 1987. Expression of ' Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation. Gene 53:1119.
79.Paddon, C. J., N. Vasantha, and R. W. Hartley. 1989. Translation and processing of Bacillus amyloliquefaciens extracellular RNase. J. Bacteriol. 171:11851187.
80. Paice, M. G.,, R. Bourbonnais,, M. Deschrochers,, L. Jurasek,, and M. Yaguchi. 1986. A xylanase gene from Bacillus subtilis: nucleotide sequence and comparison with B. pumilus gene. Arch. Microbiol. 144:201206.
81. Park, S.,, G. Liu,, T. B. Topping,, W. H. Cover,, and L. Randall. 1988. Modulation of folding pathways of exported proteins by leader sequences. Science 239:10331035.
82. Paton, J. C, B. K. May, and W. H. Elliot. 1980. Cerulenin inhibits production of extracellular proteins but not membrane proteins in Bacillus amyloliquefaciens. J. Gen. Microbiol. 118:179187.
83. Payne, M. S.,, A. Griveson,, and E. N. Jackson. Unpublished data.
84. Payne, M. S.,, and E. N. Jackson. 1991. The use of alkaline phosphatase fusions to study protein secretion in Bacillus subtilis. J. Bacteriol. 173:22782282.
85. Perlman, D.,, and H. O. Halvorson. 1983. A putative signal peptidase recognition site and sequence in eu-karyotic and prokaryotic signal peptides. J. Mol. Biol. 167:391409.
86. Petit-Glatron, M. F.,, F. Benyahia,, and R. Chambert. 1987. Secretion of Bacillus subtilis levansucrase: a possible two-step mechanism. Eur. J. Biochem. 163:379387.
87. Petit-Glatron, M. F.,, and R. Chambert. 1981. Levansucrase of Bacillus subtilis. Conclusive evidence that its production and export are unrelated to fatty-acid synthesis but modulated by membrane-modifying agents. Eur. J. Biochem. 119:603611.
88. Petit-Glatron, M. F.,, I. Monteil,, F. Benyahia,, and R. Chambert. 1990. Bacillus subtilis levansucrase: amino acid substitutions at one site affect secretion efficiency and refolding kinetics mediated by metals. Mol. Microbiol. 4:20632070.
89. Philips, G. J.,, and T. J. Silhavy. 1990. Heat-shock proteins dnaK and groEL facilitate export of LacZ hybrid proteins in E. coli. Nature (London) 344:882884.
90. Powers, S. D.,, R. M. Adams,, and J. A. Wells. 1986. Secretion and autoproteolytic maturation of subtilisin. Proc. Natl. Acad. Sci. USA 83:30963100.
91. Pugsley, A. P., C. d'Enfert, I. Reyss, and M. G. Kor-nacker. 1990. Genetics of extracellular protein secretion by gram-negative bacteria. Annu. Rev. Genet. 24: 6790.
92. Puziss, J. W.,, J. D. Fikes,, and P. J. Bassford, Jr. 1989. Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide. J. Bacteriol. 171:23032311.
93. Randall, L.,, and S. J. Hardy. 1986. Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli. Cell 46:921928.
94. Ribbe, J.,, and V. Nagarajan. 1992. Characterization of secretion efficiency of a plant signal peptide in Bacillus subtilis. Mol. Gen. Genet. 235:333339.
95. Robson, L. M.,, and G. H. Chambliss. 1987. Endo-β-l,4-glucanase gene of Bacillus subtilis DLG. J. Bacteriol. 169:20172025.
96. Sadaie, Y.,, and T. Kada. 1985. Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion. J. Bacteriol. 163:648653.
97. Sadaie, Y.,, H. Takamatsu,, K. Nakamura,, and K. Yamane. 1991. Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene. Gene 98:101105.
98. Sanders, R. L.,, and B. K. May. 1975. Evidence for extrusion of unfolded extracellular enzyme polypeptide chains through membranes of Bacillus amylolique-faciens. J. Bacterial. 123:806814.
99. Sarvas, M. 1988. Protein secretion in bacilli. Curr. Top. Microbiol. Immunol. 125:103126.
100. Sasamoto, H.,, K. Nakazawa,, K. Tsutsumi,, K. Takase,, and K. Yamane. 1989. Signal peptide of Bacillus subtilis a-amylase. J. Biochem. 106:376382.
101. Saunders, C. W.,, J. A. Pedroni,, and P. Monahan. 1991. Secretion from B. subtilis of a 34 amino acid residue fragment of human parathyroid hormone: effect of sequences adjacent to the signal sequence cleavage. Gene 102:277282.
102. Schatz, P. J.,, and J. Beckwith. 1990. Genetic analysis of protein export in Escherichia coli. Annu. Rev. Genet. 24:215249.
102a. Schmidt, A.,, M. Schiesswohl,, U. Volker,, M. Hecker,, and W. Schuman. 1992. Cloning, sequencing, mapping and transcriptional analysis of the groESL operon from Bacillus subtilis. J. Bacteriol. 174:39933999.
103. Schulein, R.,, J. Kreft,, S. Gonski,, and W. Goebel. 1991. Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme. Mol. Gen. Genet. 227:137143.
104. Shimada, H.,, M. Honjo,, I. Mita,, A. Nakayama,, A. Akaoka,, K. Manabe,, and Y. Furutani. 1985. The nucle-otide sequence and some properties of the neutral protease gene of Bacillus amyloliquefaciens. J. Bacteriol. 2:7585.
105. Silhavy, T.,, and J. R. Beckwith. 1985. Use of lac fusion for study of biological problems. Microbiol. Rev. 49: 398418.
106. Sloma, A.,, A. Ally,, D. Ally,, and J. Pero. 1988. Gene encoding a minor extracellular protease in Bacillus subtilis. J. Bacteriol. 170:55575563.
107. Sloma, A.,, C. F. Rudolph,, G. A. Rufo, Jr.,, B. J. Sullivan,, K. A. Theriault,, D. Ally,, and J. Pero. 1990. Gene encoding a novel extracellular metalloprotease in Bacillus subtilis. J. Bacteriol. 172:10241029.
108. Sloma, A.,, G. A. Rufo, Jr.,, C. F. Rudolph,, B. J. Sullivan,, K. A. Theriault,, D. Ally,, and J. Pero. 1990. Bacillopep-tidase F of Bacillus subtilis: purification of the protein and cloning of the gene. J. Bacteriol. 172:14701477.
109. Sloma, A.,, G. A. Rufo, Jr.,, K. A. Theriault,, M. Dwyer,, S. W. Wilson,, and J. Pero. 1991. Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis. J. Bacteriol. 173:68896895.
110. Smith, H.,, S. Bron,, J. Vanee,, and G. Venema. 1987. Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis. J. Bacteriol. 169:33213328.
111. Smith, H.,, A. D. Jong,, S. Bron,, and G. Venema. 1988. Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome. Gene 70:351361.
112. Smith, W. P.,, P.-C. Tai,, and B. D. Davis. 1978. Interaction of secreted nascent chains with surrounding membrane in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 75:59225925.
113. Stader, J.,, S. A. Benson,, and T. J. Silhavy. 1986. Kinetic analysis of lamB mutants suggests that signal sequence plays multiple roles in protein export. J. Biol. Chem. 262:1507515080.
114. Stahl, M. L.,, and E. Ferrari. 1984. Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation. J. Bacteriol. 158:411418.
115. Steinmetz, M.,, D. L. Coq.,, S. Aymerich,, G. Gonzy-Treboul,, and P. Gay. 1985. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansu-crase and its genetic control sites. Mol. Gen. Genet. 200:220228.
116. Stephens, M. A.,, S. A. Ortlepp,, J. F. Ollington,, and D. McConnell. 1984. Nucleotide sequence of the 5' region of the Bacillus licheniformis a-amylase gene: comparison with the B. amyloliquefaciens gene. J. Bacteriol. 158:369372.
117. Su, J.-W.,, A. Boylan,, S. M. Thomas,, K. M. Dolan,, D. B. Oliver, and C. W. Price. 1990. Isolation of secY homologue from Bacillus subtilis: evidence for a common protein export pathway in eubacteria. Mol. Microbiol. 4:305314.
118. Takagi, M.,, and T. Imanaka. 1989. Role of the pre-pro-region of neutral protease in secretion in Bacillus subtilis. J. Ferment. Bioeng. 67:7176.
119. Takagi, M.,, T. Imanaka,, and S. Aiba. 1985. Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus. J. Bacteriol. 163: 824831.
120. Takase, K.,, H. Mizuno,, and K. Yamane. 1988. NH2-terminal processing of Bacillus subtilis α-amylase. J. Biol. Chem. 263:1154811553.
121. Takekawa, S.,, N. Uozumi,, N. Tsukagoshi,, and S. Udaka. 1991. Protease involved in generation of β and α amylases from large amylase precursor in Bacillus polymyxa. J. Bacteriol. 173:68206825.
122. Takkinen, K.,, R. F. Pettersson,, N. Kalkkinen,, I. Palva,, H. Soderlund,, and L. Kaariainen. 1983. Amino acid sequence of a-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene. J. Biol. Chem. 298:10071013.
123. Tang, L.,, R. Lenstra,, T. V. Borchert,, and V. Nagarajan. 1990. Isolation and characterization of levansucrase encoding gene from Bacillus amyloliquefaciens. Gene 96:8993.
124. Thirunavukkarasu, M.,, and F. G. Priest. 1983. Synthesis of a-amylase and a-glucosidase by membrane bound ribosomes from Bacillus licheniformis. Biochem. Biophys. Res. Commun. 114:677683.
125. Tran, L.,, X. Wu,, and S. Wong. 1991. Cloning and expression of a novel protease gene encoding an extracellular neutral protease from Bacillus subtilis. J. Bacteriol. 173:63646372.
126. Tsuboi, A.,, R. Uchihi,, T. Adachi,, T. Sasaki,, S. Haya-kawa,, H. Yamagata,, N. Tsukagoshi,, and S. Udaka. 1988. Characterization of the genes for the hexagonally arranged surface layer proteins in protein-producing Bacillus brevis 47: complete nucleotide sequence of the middle wall protein gene. J. Bacteriol. 170:935945.
127. Tsukagoshi, N.,, R. Tabata,, T. Takemura,, H. Yamagata,, and S. Udaka. 1984. Molecular cloning of a major cell wall protein gene from protein-producing Bacillus brevis 47 and its expression in Escherichia coli and Bacillus subtilis. J. Bacteriol. 158:10541060.
128. Uozumi, N.,, K. Sakurai,, T. Sasaki,, S. Takekawa,, H. Yamagata,, N. Tsukagoshi,, and S. Udaka. 1989. A single gene directs synthesis of a precursor protein with β-and a-amylase activities in Bacillus polymyxa. J. Bacteriol. 171:375382.
129. Van Den Burg, B.,, H. G. Enequist,, M. E. Van Der Haar,, V. G. H. Eljsink,, B. K. Stulp,, and G. Venema. 1991. A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product. J. Bacteriol. 173:41074115.
129a. van der Wolk, J.,, M. Klose,, E. Breukink,, R. A. Demel,, B. Kruijff,, R. Freudl,, and A. J. M. Driessen. Characterization of a Bacillus subtilis secA mutant protein deficient in translocation ATPase and release from the membrane. Submitted for publication.
130. van Dijl, J. M.,, A. de Jong,, G. Venema,, and S. Bron. 1992. Signal peptidase I of Bacillus subtilis: patterns of conserved amino acids in prokaryotic and eukaryotic type I signal peptidases. EMBO J. 11:28192828.
131. Vasantha, N.,, and L. D. Thompson. 1986. Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis. Gene 49:2328.
132. Vasantha, N.,, L. D. Thompson,, C. Rhodes,, C. Banner,, J. Nagle,, and D. Filpula. 1984. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequences and mature protein. J. Bacteriol. 159:811819.
133. vonHeljne, G. 1983. Patterns of amino acids near signal sequence cleavage sites. Eur. J. Biochem. 133:1721.
134. von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14: 46834690.
135. Wagner, B.,, M. Wagner,, L. Wollweber,, and D. Behnke. 1989. Immunoelectron microscopy of Bacillus subtilis cell secreting human interferon al or staphylokinase. FEMS Microbiol. Lett. 65:327332.
136. Wetzsteln, M.,, J. Dedio,, and W. Schumann. 1990. Complete nucleotide sequence of the Bacillus subtilis dnaK gene. Nucleic Acids Res. 18:2172.
137. Wlckner, W.,, A. J. M. Driessen,, and F. Hard. 1991. The enzymology of protein translocation across the Escherichia coli plasma membrane. Artnu. Rev. Biochem. 60:101124.
138. Wu, J. J.,, and P. J. Piggot,. 1989. Regulation of late expression of the Bacillus subtilis spoil A locus: evidence that it is cotranscribed with the gene for a putative penicillin binding protein, p. 321328. In M. Zukowski,, A. T. Ganesan,, and J. Hoch (ed.), Genetics and Biotechnology of Bacilli. Academic Press, Inc., New York.
139. Yang, M. Y.,, E. Ferrari,, and D. J. Henner. 1984. Cloning of the neutral protease gene of Bacillus subtilis and use of the cloned gene to create an in vitro-derived deletion mutation. J. Bacteriol. 160:1521.
140. Zagorec, M.,, and M. Steinmetz. 1990. Expression of levansucrase-β-galactosidase hybrids inhibits secretion and is lethal in Bacillus subtilis. J. Gen. Microbiol. 136:11371143.
141. Zhu, X.,, Y. Ohta,, F. Jordan,, and M. Inouye. 1989. Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intramolecular process. Nature (London) 339:483484.


Generic image for table
Table 1

Signal peptide sequences

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49
Generic image for table
Table 2

Lipoprotein signal sequences

Citation: Nagarajan V. 1993. Protein Secretion, p 713-726. In Sonenshein A, Hoch J, Losick R (ed), and Other Gram-Positive Bacteria. ASM Press, Washington, DC. doi: 10.1128/9781555818388.ch49

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