Chapter 12 : Enterohemorrhagic

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Gastrointestinal pathogenic includes enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroinvasive (EIEC), enteroaggregative (EAEC), and enterohemorrhagic (EHEC). This chapter focuses largely on the EHEC group, which among the strains that cause foodborne illness, is the most significant group based on the frequency of foodborne illness in the United States and the severity of illness. Some types of EPEC are referred to as enteroadherent (EAEC), based on specific patterns of adherence. The chapter next discusses the characteristics of O157:H7, first identified as a foodborne pathogen in 1982. The serious nature of the symptoms of hemorrhagic colitis and hemolytic-uremic syndrome (HUS) caused by O157:H7 places this pathogen in a category apart from other foodborne pathogens, which typically cause only mild symptoms. The severity of the illness it causes combined with its apparent low infectious dose qualifies O157:H7 to be among the most serious of known foodborne pathogens. Cattle are a major reservoir of O157:H7. O157:H7 is still by far the most important serotype of Shiga toxins (Stx)-producing (STEC) in North America. The increased availability in clinical laboratories of techniques such as testing for Stxs or their genes and identification of other virulence markers unique for EHEC will continue to enhance the detection of disease attributable to non-O157 EHEC.

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Figure 12.1

Number of Stx-producing O157:H7 cases in the United States by year, 2000 to 2010. doi:10.1128/9781555818463.ch12f1

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Figure 12.2

Schematic illustration of A/E lesion formation in EHEC, modified from reference . (A) A/E translocation of effector proteins through T3SS that forms a pore through the membranes of EHEC. EHEC translocate a number of proteins: EspB and EspD, which form a translocon in the plasma membrane; the cytoplasmic proteins EspF, G, and Map; the translocated intimin receptor Tir, which inserts into the plasma membrane; and other unidentified effectors. (B) Formation of EHEC pedestal. EHEC intimately attaches to the host cell through intimin-Tir binding. The binding triggers the formation of actin-rich pedestals beneath adherent bacteria after Wiskott-Aldrich syndrome protein (WASP) and the heptameric actin-related protein Arp2/3 are recruited to the pedestal tip. doi:10.1128/9781555818463.ch12f2

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Figure 12.3

Genetic organization of the EHEC LEE and EHEC prophages CP-933U, CP-933K, and CP-933P, reproduced from reference . doi:10.1128/9781555818463.ch12f3

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Figure 12.4

T3SS apparatus of EHEC. The basal body of the T3SS is composed of the secretin EscC, the inner membrane proteins EscR, EscS, EscT, EscU, and EscV, and the EscJ lipoprotein, which connects the inner and outer membrane ring structures. EscF constitutes the needle structure, whereas EspA subunits polymerize to form the EspA filament. EspB and EspD form the translocation pore in the host cell plasma membrane, connecting the bacteria with the eukaryotic cell via EspA filaments. The cytoplasmic ATPase EscN provides the energy to the system by hydrolyzing ATP molecules into ADP. SepD and SepL have been represented as cytoplasmic components of the T3SS. (Reproduced from reference .) doi:10.1128/9781555818463.ch12f4

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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1. Ballmer, K.,, B. M. Korczak,, P. Kuhnert,, P. Slickers,, R. Ehricht,, and H. Hachler. 2007. Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray. J. Clin. Microbiol. 45:370379.
2. Barkocy-Gallagher, G. A.,, T. M. Arthur,, M. Rivera-Betancourt,, X. Nou,, S. D. Shackelford,, T. L. Wheeler,, and M. Koohmaraie. 2003. Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. J. Food Prot. 66:19781986.
3. Baylis, C. L.,, S. MacPhee,, A. J. Robinson,, R. Griffiths,, K. Lilley,, and R. P. Betts. 2004. Survival of Escherichia coli O157:H7, O111:H- and O26:H11 in artificially contaminated chocolate and confectionery products. Int. J. Food Microbiol. 96:3548.
4. Bell, B. P.,, M. Goldoft,, P. M. Griffin,, M. A. Davis,, D. C. Gordon,, P. I. Tarr,, C. A. Bartleson,, J. H. Lewis,, T. J. Barrett,, J. G. Wells, et al. 1994. A multistate outbreak of Escherichia coli O157:H7-associated bloody diarrhea and hemolytic uremic syndrome from hamburgers. The Washington experience. JAMA 272:13491353.
5. Besser, R. E.,, P. M. Griffin,, and L. Slutsker. 1999. Escherichia coli O157:H7 gastroenteritis and the hemolytic uremic syndrome: an emerging infectious disease. Annu. Rev. Med. 50:355367.
6. Besser, T. E.,, D. D. Hancock,, L. C. Pritchett,, E. M. McRae,, D. H. Rice,, and P. I. Tarr. 1997. Duration of detection of fecal excretion of Escherichia coli O157:H7 in cattle. J. Infect. Dis. 175:726729.
7. Bettelheim, K. A. 2007. The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens. Crit. Rev. Microbiol. 33:6787.
8. Beutin, L.,, D. Geier,, H. Steinruck,, S. Zimmermann,, and F. Scheutz. 1993. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J. Clin. Microbiol. 31:24832488.
9. Brackett, R.,, Y. Hao,, and M. Doyle. 1994. Ineffectiveness of hot acid sprays to decontaminate Escherichia coli O157:H7 on beef. J. Food Prot. 57:198203.
10. Brashears, M. M.,, A. Amezquita,, and D. Jaroni. 2005. Lactic acid bacteria and their uses in animal feeding to improve food safety. Adv. Food Nutr. Res. 50:131.
11. Brett, K. N.,, M. A. Hornitzky,, K. A. Bettelheim,, M. J. Walker,, and S. P. Djordjevic. 2003. Bovine non-O157 Shiga toxin 2-containing Escherichia coli isolates commonly possess stx2-EDL933 and/or stx2vhb subtypes. J. Clin. Microbiol. 41:27162722.
12. Brooks, J. T.,, E. G. Sowers,, J. G. Wells,, K. D. Greene,, P. M. Griffin,, R. M. Hoekstra,, and N. A. Strockbine. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192:14221429.
13.Reference deleted.
14. Burk, C.,, R. Dietrich,, G. Acar,, M. Moravek,, M. Bulte,, and E. Martlbauer. 2003. Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin. J. Clin. Microbiol. 41:21062112.
15. Burland, V.,, Y. Shao,, N. T. Perna,, G. Plunkett,, H. J. Sofia,, and F. R. Blattner. 1998. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26:41964204.
16. Calderon, V. E.,, Q. Chang,, M. McDermott,, M. B. Lytle,, G. McKee,, K. Rodriguez,, D. A. Rasko,, V. Sperandio,, and A. G. Torres. 2010. Outbreak caused by cad-negative Shiga toxin-producing Escherichia coli O111, Oklahoma. Foodborne Pathog. Dis. 7:107109.
17. Callaway, T. R.,, R. C. Anderson,, K. J. Genovese,, T. L. Poole,, T. J. Anderson,, J. A. Byrd,, L. F. Kubena,, and D. J. Nisbet. 2002. Sodium chlorate supplementation reduces E. coli O157:H7 populations in cattle. J. Anim. Sci. 80:16831689.
18. Campellone, K. G.,, and J. M. Leong. 2003. Tails of two Tirs: actin pedestal formation by enteropathogenic E. coli and enterohemorrhagic E. coli O157:H7. Curr. Opin. Microbiol. 6:8290.
19.Centers for Disease Control and Prevention. 2006. Importance of culture confirmation of Shiga toxin-producing Escherichia coli infection as illustrated by outbreaks of gastroenteritis—New York and North Carolina, 2005. MMWR Morb. Mortal. Wkly. Rep. 55:10421045.
20.Centers for Disease Control and Prevention. 1996. Lake-associated outbreak of Escherichia coli O157:H7—Illinois, 1995. JAMA 275:18721873.
21.Centers for Disease Control and Prevention. 1999. Outbreak of Escherichia coli O157:H7 and Campylobacter among attendees of the Washington County Fair—New York, 1999. MMWR Morb. Mortal. Wkly. Rep. 48:803805.
22. Cernicchiaro, N.,, D. L. Pearl,, S. A. McEwen,, H. N. Zerby,, F. L. Fluharty,, S. C. Loerch,, M. D. Kauffman,, J. L. Bard,, and J. T. LeJeune. 2010. A randomized controlled trial to assess the impact of dietary energy sources, feed supplements, and the presence of super-shedders on the detection of Escherichia coli O157:H7 in feedlot cattle using different diagnostic procedures. Foodborne Pathog. Dis. 7:10711081.
23. Chaudhuri, R. R.,, N. J. Loman,, L. A. Snyder,, C. M. Bailey,, D. J. Stekel,, and M. J. Pallen. 2008. xBASE2: a comprehensive resource for comparative bacterial genomics. Nucleic Acids Res. 36:D543D546.
24. Clavero, M.,, J. Monk,, L. Beuchat,, M. Doyle,, and R. Brackett. 1994. Inactivation of Escherichia coli O157:H7, salmonellae, and Campylobacter jejuni in raw ground beef by gamma irradiation. Appl. Environ. Microbiol. 60:20692075.
25. Cobbold, R.,, and P. Desmarchelier. 2000. A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian diary herds. Vet. Microbiol. 71:125137.
26. Cohen, A.,, G. E. Hannigan,, B. R. Williams,, and C. A. Lingwood. 1987. Roles of globotriosyl- and galabiosylceramide in verotoxin binding and high affinity interferon receptor. J. Biol. Chem. 262:1708817091.
27. D‘Aoust, J.,, C. Park,, R. Szabo,, E. Todd,, B. Emmons,, and R. McKellar. 1988. Thermal inactivation of Campylobacter species, Yersinia enterocolitca, and hemorrhagic Escherichia coli O157:H7 in fluid milk. J. Dairy Sci. 71:32303236.
28. David, S. T.,, L. MacDougall,, K. Louie,, L. McIntyre,, A. M. Paccagnella,, S. Schleicher,, and A. Hamade. 2004. Petting zoo-associated Escherichia coli O157:H7—secondary transmission, asymptomatic infection, and prolonged shedding in the classroom. Can. Commun. Dis. Rep. 30:173180.
29. Davis, M. A.,, D. H. Rice,, H. Sheng,, D. D. Hancock,, T. E. Besser,, R. Cobbold,, and C. J. Hovde. 2006. Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers. Appl. Environ. Microbiol. 72:37663770.
30. Denno, D. M.,, W. E. Keene,, C. M. Hutter,, J. K. Koepsell,, M. Patnode,, D. Flodin-Hursh,, L. K. Stewart,, J. S. Duchin,, L. Rasmussen,, R. Jones,, and P. I. Tarr. 2009. Tri-county comprehensive assessment of risk factors for sporadic reportable bacterial enteric infection in children. J. Infect. Dis. 199:467476.
31. Dodd, C. C.,, M. W. Sanderson,, J. M. Sargeant,, T. G. Nagaraja,, R. D. Oberst,, R. A. Smith,, and D. D. Griffin. 2003. Prevalence of Escherichia coli O157 in cattle feeds in Midwestern feedlots. Appl. Environ. Microbiol. 69:52435247.
32. Donnenberg, M. S.,, and T. S. Whittam. 2001. Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. J. Clin. Investig. 107:539548.
33. Doyle, M. P.,, and J. L. Schoeni. 1984. Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis. Appl. Environ. Microbiol. 48:855856.
34. Elder, R. O.,, J. E. Keen,, G. R. Siragusa,, G. A. Barkocy-Gallagher,, M. Koohmaraie,, and W. W. Laegreid. 2000. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proc. Natl. Acad. Sci. USA 97:29993003.
35. Elliott, S. J.,, L. A. Wainwright,, T. K. McDaniel,, K. G. Jarvis,, Y. K. Deng,, L. C. Lai,, B. P. McNamara,, M. S. Donnenberg,, and J. B. Kaper. 1998. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol. Microbiol. 28:14.
36. Espie, E.,, F. Grimont,, V. Vaillant,, M. P. Montet,, I. Carle,, C. Bavai,, H. de Valk,, and C. Vernozy-Rozand. 2006. O148 Shiga toxin-producing Escherichia coli outbreak: microbiological investigation as a useful complement to epidemiological investigation. Clin. Microbiol. Infect. 12:992998.
37. Ethelberg, S.,, B. Smith,, M. Torpdahl,, M. Lisby,, J. Boel,, T. Jensen,, and K. Molbak. 2007. An outbreak of Verocytotoxin-producing Escherichia coli O26:H11 caused by beef sausage, Denmark 2007. Euro Surveill. 12:E070531.4.
38. Ethelberg, S.,, B. Smith,, M. Torpdahl,, M. Lisby,, J. Boel,, T. Jensen,, E. M. Nielsen,, and K. Molbak. 2009. Outbreak of non-O157 Shiga toxin-producing Escherichia coli infection from consumption of beef sausage. Clin. Infect. Dis. 48:e78e81.
39. Foster, J. W. 2004. Escherichia coli acid resistance: tales of an amateur acidophile. Nat. Rev. Microbiol. 2:898907.
40. Frank, C.,, D. Werber,, J. P. Cramer,, M. Askar,, M. Faber,, M. A. Heiden,, H. Bernard,, A. Fruth,, R. Prager,, A. Spode,, M. Wadl,, A. Zoufaly,, S. Jordan,, K. Stark,, and G. Krause. 2011. Epidemic profile of Shiga toxin-producing Escherichia coli O104:H4 outbreak in Germany—preliminary report. N. Engl. J. Med. http://www.nejm.org/doi/full/10.1056/NEJMoa1106483. Accessed 16 July 2011.
41. Fuller, C. A.,, C. A. Pellino,, M. J. Flagler,, J. E. Strasser,, and A. A. Weiss. 2011. Shiga toxin subtypes display dramatic differences in potency. Infect. Immun. 79:13291337.
42. Gannon, V. P.,, C. Teerling,, S. A. Masri,, and C. L. Gyles. 1990. Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin II family. J. Gen. Microbiol. 136:11251135.
43. Garcia-Aljaro, C.,, M. Muniesa,, J. E. Blanco,, M. Blanco,, J. Blanco,, J. Jofre,, and A. R. Blanch. 2005. Characterization of Shiga toxin-producing Escherichia coli isolated from aquatic environments. FEMS Microbiol. Lett. 246:5565.
44. Garmendia, J.,, G. Frankel,, and V. F. Crepin. 2005. Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation. Infect. Immun. 73:25732585.
45. Glass, K.,, J. Loeffelholz,, J. Ford,, and M. Doyle. 1992. Fate of Escherichia coli O157:H7 as affected by pH or sodium chloride in fermented, dry sausage. Appl. Environ. Microbiol. 58:25132516.
46. Griffin, P. M.,, and R. V. Tauxe. 1991. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol. Rev. 13:6098.
47. Hancock, D.,, T. Besser,, J. Lejeune,, M. Davis,, and D. Rice. 2001. The control of VTEC in the animal reservoir. Int. J. Food Microbiol. 66:7178.
48. Hedican, E. B.,, C. Medus,, J. M. Besser,, B. A. Juni,, B. Koziol,, C. Taylor,, and K. E. Smith. 2009. Characteristics of O157 versus non-O157 Shiga toxin-producing Escherichia coli infections in Minnesota, 2000-2006. Clin. Infect. Dis. 49:358364.
49. Hiramatsu, R.,, M. Matsumoto,, K. Sakae,, and Y. Miyazaki. 2005. Ability of Shiga toxin-producing Escherichia coli and Salmonella spp. to survive in a desiccation model system and in dry foods. Appl. Environ. Microbiol. 71:66576663.
50. Hrudey, S. E.,, P. Payment,, P. M. Huck,, R. W. Gillham,, and E. J. Hrudey. 2003. A fatal waterborne disease epidemic in Walkerton, Ontario: comparison with other waterborne outbreaks in the developed world. Water Sci. Technol. 47:714.
51. Jacob, M. E.,, J. T. Fox,, J. S. Drouillard,, D. G. Renter,, and T. G. Nagaraja. 2008. Effects of dried distillers grain on fecal prevalence and growth of Escherichia coli O157 in batch culture fermentations from cattle. Appl. Environ. Microbiol. 74:3843.
52. Jacob, M. E.,, Z. D. Paddock,, D. G. Renter,, K. F. Lechtenberg,, and T. G. Nagaraja. 2010. Inclusion of dried or wet distillers’ grains at different levels in diets of feedlot cattle affects fecal shedding of Escherichia coli O157:H7. Appl. Environ. Microbiol. 76:72387242.
53. Johnson, T. J.,, and L. K. Nolan. 2009. Pathogenomics of the virulence plasmids of Escherichia coli. Microbiol. Mol. Biol. Rev. 73:750774.
54. Johnson, W. M.,, H. Lior,, and G. S. Bezanson. 1983. Cytotoxic Escherichia coli O157:H7 associated with haemorrhagic colitis in Canada. Lancet i:76.
55. Kaper, J. B.,, J. P. Nataro,, and H. L. Mobley. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2:123140.
56. Karch, H.,, H. Russmann,, H. Schmidt,, A. Schwarzkopf,, and J. Heesemann. 1995. Long-term shedding and clonal turnover of enterohemorrhagic Escherichia coli O157 in diarrheal diseases. J. Clin. Microbiol. 33:16021605.
57. Karmali, M. A. 2009. Host and pathogen determinants of verocytotoxin-producing Escherichia coli-associated hemolytic uremic syndrome. Kidney Int. Suppl. 2009(112):S4S7.
58. Karmali, M. A.,, M. Petric,, C. Lim,, P. C. Fleming,, G. S. Arbus,, and H. Lior. 1985. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J. Infect. Dis. 151:775782.
59. Kim, H. H.,, M. Samadpour,, L. Grimm,, C. R. Clausen,, T. E. Besser,, M. Baylor,, J. M. Kobayashi,, M. A. Neill,, F. D. Schoenknecht,, and P. I. Tarr. 1994. Characteristics of antibiotic-resistant Escherichia coli O157:H7 in Washington State, 1984-1991. J. Infect. Dis. 170:16061609.
60. King, L. A.,, I. Filliol-Toutain,, P. Mariani-Kurkidjian,, V. Vaillant,, C. Vernozy-Rozand,, S. Ganet,, N. Pihier,, P. Niaudet,, and H. de Valk. 2010. Family outbreak of Shiga toxin-producing Escherichia coli O123:H-, France, 2009. Emerg. Infect. Dis. 16:14911493.
61. Koehler, K. M.,, T. Lasky,, S. B. Fein,, S. M. Delong,, M. A. Hawkins,, T. Rabatsky-Ehr,, S. M. Ray,, B. Shiferaw,, E. Swanson,, and D. J. Vugia. 2006. Population-based incidence of infection with selected bacterial enteric pathogens in children younger than five years of age, 1996-1998. Pediatr. Infect. Dis. J. 25:129134.
62. LeJeune, J. T.,, T. E. Besser,, and D. D. Hancock. 2001. Cattle water troughs as reservoirs of Escherichia coli O157. Appl. Environ. Microbiol. 67:30533057.
63. LeJeune, J. T.,, D. Hancock,, Y. Wasteson,, E. Skjerve,, and A. M. Urdahl. 2006. Comparison of E. coli O157 and Shiga toxin-encoding genes (stx) prevalence between Ohio, USA and Norwegian dairy cattle. Int. J. Food Microbiol. 109:1924.
64. LeJeune, J. T.,, and A. N. Wetzel. 2007. Preharvest control of Escherichia coli O157 in cattle. J. Anim. Sci. 85:E73E80.
65. Licence, K.,, K. R. Oates,, B. A. Synge,, and T. M. Reid. 2001. An outbreak of E. coli O157 infection with evidence of spread from animals to man through contamination of a private water supply. Epidemiol. Infect. 126:135138.
66. Lin, J.,, M. Smith,, K. Chapin,, H. Baik,, G. Bennett,, and J. Foster. 1996. Mechanisms of acid resistance in enterohemorrhagic Escherichia coli. Appl. Environ. Microbiol. 62:30943100.
67. Line, J.,, A. Fain,, A. Moran,, L. Martin,, R. Lechowich,, J. Carosella,, and W. Brown. 1991. Lethality of heat to Escherichia coli O157:H7: D-value and z-value determinations in ground beef. J. Food Prot. 54:762766.
68. Lingwood, C. A. 1996. Role of verotoxin receptors in pathogenesis. Trends Microbiol. 4:147153.
69. Lynn, T. V.,, D. D. Hancock,, T. E. Besser,, J. H. Harrison,, D. H. Rice,, N. T. Stewart,, and L. L. Rowan. 1998. The occurrence and replication of Escherichia coli in cattle feeds. J. Dairy Sci. 81:11021108.
70. Mathusa, E. C.,, Y. Chen,, E. Enache,, and L. Hontz. 2010. Non-O157 Shiga toxin-producing Escherichia coli in foods. J. Food Prot. 73:17211736.
71. McNeilly, T. N.,, M. C. Mitchell,, A. J. Nisbet,, S. McAteer,, C. Erridge,, N. F. Inglis,, D. G. Smith,, J. C. Low,, D. L. Gally,, J. F. Huntley,, and A. Mahajan. 2010. IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling. Vaccine 28:14121421.
72. Melton-Celsa, A.,, and A. O‘Brien,. 1998. Structure, biology, and relative toxicity of Shiga toxin family members for cells and animals, p. 121128. In J. Kaper, and A. O’Brien (ed.), Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. coli Strains. ASM Press, Washington, DC.
73. Meng, J.,, and M. P. Doyle,. 1998. Microbiology of Shiga toxin-producing Escherichia coli in foods, p. 92111. In J. Kaper, and A. O’Brien (ed.), Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. coli Strains. ASM Press, Washington, DC.
74. Meng, J.,, S. Zhao,, M. Doyle,, and S. Joseph. 1998. Antibiotic resistance of Escherichia coli O157:H7 and O157:NM isolated from animals, food and humans. J. Food Prot. 61:15111514.
75. Meyer-Broseta, S.,, S. N. Bastian,, P. D. Arne,, O. Cerf,, and M. Sanaa. 2001. Review of epidemiological surveys on the prevalence of contamination of healthy cattle with Escherichia coli serogroup O157:H7. Int. J. Hyg. Environ. Health 203:347361.
76. Michino, H.,, K. Araki,, S. Minami,, S. Takaya,, N. Sakai,, M. Miyazaki,, A. Ono,, and H. Yanagawa. 1999. Massive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts. Am. J. Epidemiol. 150:787796.
77. Muller, E. E.,, M. M. Ehlers,, and W. O. Grabow. 2001. The occurrence of E. coli O157:H7 in South African water sources intended for direct and indirect human consumption. Water Res. 35:30853088.
78. Nataro, J. P.,, and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142201.
79. Naylor, S. W.,, J. C. Low,, T. E. Besser,, A. Mahajan,, G. J. Gunn,, M. C. Pearce,, I. J. McKendrick,, D. G. Smith,, and D. L. Gally. 2003. Lymphoid follicle-dense mucosa at the terminal rectum is the principal site of colonization of enterohemorrhagic Escherichia coli O157:H7 in the bovine host. Infect. Immun. 71:15051512.
80. Nielsen, E. M.,, M. N. Skov,, J. J. Madsen,, J. Lodal,, J. B. Jespersen,, and D. L. Baggesen. 2004. Verocytotoxin-producing Escherichia coli in wild birds and rodents in close proximity to farms. Appl. Environ. Microbiol. 70:69446947.
81. O’Brien, A. D.,, V. L. Tesh,, A. Donohue-Rolfe,, M. P. Jackson,, S. Olsnes,, K. Sandvig,, A. A. Lindberg,, and G. T. Keusch. 1992. Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Curr. Top. Microbiol. Immunol. 180:6594.
82. Ogura, Y.,, T. Ooka,, Asadulghani,, J. Terajima,, J. P. Nougayrede,, K. Kurokawa,, K. Tashiro,, T. Tobe,, K. Nakayama,, S. Kuhara,, E. Oswald,, H. Watanabe,, and T. Hayashi. 2007. Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes. Genome Biol. 8:R138.
83. Ogura, Y.,, T. Ooka,, A. Iguchi,, H. Toh,, M. Asadulghani,, K. Oshima,, T. Kodama,, H. Abe,, K. Nakayama,, K. Kurokawa,, T. Tobe,, M. Hattori,, and T. Hayashi. 2009. Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc. Natl. Acad. Sci. USA 106:1793917944.
84. Orr, P.,, D. Milley,, D. Coiby,, and M. Fast. 1994. Prolonged fecal excretion of verotoxin-producing Escherichia coli following diarrheal illness. Clin. Infect. Dis. 19:796797.
85. Orskov, F.,, I. Orskov,, and J. A. Villar. 1987. Cattle as reservoir of verotoxin-producing Escherichia coli O157:H7. Lancet ii:276.
86. Orskov, I.,, F. Orskov,, B. Jann,, and K. Jann. 1977. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol. Rev. 41:667710.
87. Orth, D.,, K. Grif,, A. B. Khan,, A. Naim,, M. P. Dierich,, and R. Wurzner. 2007. The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome. Diagn. Microbiol. Infect. Dis. 59:235242.
88. Oswald, E.,, H. Schmidt,, S. Morabito,, H. Karch,, O. Marches,, and A. Caprioli. 2000. Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect. Immun. 68:6471.
89. Piercefield, E. W.,, K. K. Bradley,, R. L. Coffman,, and S. M. Mallonee. 2010. Hemolytic uremic syndrome after an Escherichia coli O111 outbreak. Arch. Intern. Med. 170:16561663.
90. Rangel, J. M.,, P. H. Sparling,, C. Crowe,, P. M. Griffin,, and D. L. Swerdlow. 2005. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002. Emerg. Infect. Dis. 11:603609.
91. Renter, D. G.,, V. Bohaychuk,, J. Van Donkersgoed,, and R. King. 2007. Presence of non-O157 Shiga toxin-producing Escherichia coli in feces from feedlot cattle in Alberta and absence on corresponding beef carcasses. Can. J. Vet. Res. 71:230235.
92. Rice, D. H.,, K. M. McMenamin,, L. C. Pritchett,, D. D. Hancock,, and T. E. Besser. 1999. Genetic subtyping of Escherichia coli O157 isolates from 41 Pacific Northwest USA cattle farms. Epidemiol. Infect. 122:479484.
93.Robert Koch Institut. 2011. Technical report: EHEC/HUS O104:H4 outbreak, Germany, May/June 2011. http://www.rki.de/cln_160/nn_217400/EN/Home/EHEC__Report,templateId=raw,property=publicationFile.pdf/EHEC_Report.pdf. Accessed 16 July 2011.
94. Sandvig, K.,, and B. van Deurs. 1996. Endocytosis, intracellular transport, and cytotoxic action of Shiga toxin and ricin. Physiol. Rev. 76:949966.
95. Sargeant, J. M.,, M. W. Sanderson,, D. D. Griffin,, and R. A. Smith. 2004. Factors associated with the presence of Escherichia coli O157 in feedlot-cattle water and feed in the Midwestern USA. Prev. Vet. Med. 66:207237.
96. Scallan, E.,, R. M. Hoekstra,, F. J. Angulo,, R. V. Tauxe,, M. A. Widdowson,, S. L. Roy,, J. L. Jones,, and P. M. Griffin. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17:715.
97. Schmidt, H.,, J. Scheef,, S. Morabito,, A. Caprioli,, L. H. Wieler,, and H. Karch. 2000. A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl. Environ. Microbiol. 66:12051208.
98. Schmidt, M. A. 2010. LEEways: tales of EPEC, ATEC and EHEC. Cell Microbiol. 12:15441552.
99. Schroeder, C. M.,, J. Meng,, S. Zhao,, C. DebRoy,, J. Torcolini,, C. Zhao,, P. F. McDermott,, D. D. Wagner,, R. D. Walker,, and D. G. White. 2002. Antimicrobial resistance of Escherichia coli O26, O103, O111, O128, and O145 from animals and humans. Emerg. Infect. Dis. 8:14091414.
100. Schroeder, C. M.,, C. Zhao,, C. DebRoy,, J. Torcolini,, S. Zhao,, D. G. White,, D. D. Wagner,, P. F. McDermott,, R. D. Walker,, and J. Meng. 2002. Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Appl. Environ. Microbiol. 68:576581.
101. Sekse, C.,, K. O‘Sullivan,, P. E. Granum,, L. M. Rorvik,, Y. Wasteson,, and H. J. Jorgensen. 2009. An outbreak of Escherichia coli O103:H25— bacteriological investigations and genotyping of isolates from food. Int. J. Food Microbiol. 133:259264.
102. Smith, D. R.,, R. A. Moxley,, T. J. Klopfenstein,, and G. E. Erickson. 2009. A randomized longitudinal trial to test the effect of regional vaccination within a cattle feedyard on Escherichia coli O157:H7 rectal colonization, fecal shedding, and hide contamination. Foodborne Pathog. Dis. 6:885892.
103. Tarr, P. I.,, C. A. Gordon,, and W. L. Chandler. 2005. Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet 365:10731086.
104. Tesh, V. L.,, and A. D. O‘Brien. 1991. The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins. Mol. Microbiol. 5:18171822.
105. Teunis, P.,, K. Takumi,, and K. Shinagawa. 2004. Dose response for infection by Escherichia coli O157:H7 from outbreak data. Risk Anal. 24:401407.
106. van Setten, P. A.,, L. A. Monnens,, R. G. Verstraten,, L. P. van den Heuvel,, and V. W. van Hinsbergh. 1996. Effects of verocytotoxin-1 on nonadherent human monocytes: binding characteristics, protein synthesis, and induction of cytokine release. Blood 88:174183.
107. Vasconcelos, J. T.,, N. A. Elam,, M. M. Brashears,, and M. L. Galyean. 2008. Effects of increasing dose of live cultures of Lactobacillus acidophilus (strain NP 51) combined with a single dose of Propionibacterium freudenreichii (strain NP 24) on performance and carcass characteristics of finishing beef steers. J. Anim. Sci. 86:756762.
108. Vu-Khac, H.,, and N. A. Cornick. 2008. Prevalence and genetic profiles of Shiga toxin-producing Escherichia coli strains isolated from buffaloes, cattle, and goats in central Vietnam. Vet. Microbiol. 126:356363.
109. Weinstein, D. L.,, M. P. Jackson,, J. E. Samuel,, R. K. Holmes,, and A. D. O’Brien. 1988. Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J. Bacteriol. 170:42234230.
110. Wells, J. G.,, B. R. Davis,, I. K. Wachsmuth,, L. W. Riley,, R. S. Remis,, R. Sokolow,, and G. K. Morris. 1983. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18:512520.
111. Williams, M. S.,, J. L. Withee,, E. D. Ebel,, N. E. Bauer, Jr.,, W. D. Schlosser,, W. T. Disney,, D. R. Smith,, and R. A. Moxley. 2010. Determining relationships between the seasonal occurrence of Escherichia coli O157:H7 in live cattle, ground beef, and humans. Foodborne Pathog. Dis. 7:12471254.
112. Wilson, J. B.,, R. C. Clarke,, S. A. Renwick,, K. Rahn,, R. P. Johnson,, M. A. Karmali,, H. Lior,, D. Alves,, C. L. Gyles,, K. S. Sandhu,, S. A. McEwen,, and J. S. Spika. 1996. Vero cytotoxigenic Escherichia coli infection in dairy farm families. J. Infect. Dis. 174:10211027.
113. Zhang, W.,, M. Bielaszewska,, T. Kuczius,, and H. Karch. 2002. Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx1c) in Escherichia coli strains isolated from humans. J. Clin. Microbiol. 40:14411446.
114. Zhao, T.,, and M. Doyle. 1994. Fate of enterohemorrhagic Escherichia coli O157:H7 in commercial mayonnaise. J. Food Prot. 57:780783.
115. Zhao, T.,, M. Doyle,, and R. Besser. 1993. Fate of enterohemorrhagic Escherichia coli O157:H7 in apple cider with and without preservatives. Appl. Environ. Microbiol. 59:25262530.
116. Zweifel, C.,, N. Giezendanner,, S. Corti,, G. Krause,, L. Beutin,, J. Danuser,, and R. Stephan. 2010. Characteristics of Shiga toxin-producing Escherichia coli isolated from Swiss raw milk cheese within a 3-year monitoring program. J. Food Prot. 73:8891.


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Table 12.1

Serotypes of non-O157 Stx-producing recovered from patients with hemorrhagic colitis and/or HUS

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Table 12.2

Comparison of values for O157:H7 and spp. in ground beef

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Table 12.3

Vehicles of foodborne outbreaks and associated cases of O157 infections in the United States between 2000 and 2010

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
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Table 12.4

Representative foodborne and waterborne outbreaks of O157:H7 and other EHEC infections

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12
Generic image for table
Table 12.5

Nomenclature and biological characteristics of Stxs

Citation: Meng J, LeJeune J, Zhao T, Doyle M. 2013. Enterohemorrhagic , p 287-309. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch12

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