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Chapter 28 : Protozoan Parasites

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Abstract:

Protozoan parasites have long been associated with foodborne and waterborne outbreaks of disease in humans. Difficulties arise with the inactivation of these organisms because of their resistance to environmental stresses. A major characteristic of apicomplexan parasites is that a vertebrate host is required to complete the complex life cycle and produce infectious cysts. Of this group, species, , and , and frequently inhabit the intestinal mucosa and produce diarrheal illnesses in humans. The life cycle stages of apicomplexan parasites are produced intracellularly in the host. For , , and , sporogony typically occurs outside the host, requiring the passage of time before oocysts are infective to a new host, while oocysts excreted already are sporulated and are infectious when shed. Cryptosporidiosis is acquired after ingesting food or water contaminated with infective oocysts. Cyclosporiasis is characterized by mild to severe nausea, anorexia, abdominal cramping, mild fever, and watery diarrhea. The only successful antimicrobial treatment for is trimethoprim-sulfamethoxazole (TMP-SMX). Toxoplasmosis can be acquired by ingestion of lamb, poultry, horse, and wild game animals. Patients with muscular sarcocystosis present with musculoskeletal pain, fever, rash, cardiomyopathy, bronchospasm, and subcutaneous swelling. Inactivation of the protozoan parasites has been a challenging task. Molecular tools such as PCR, restriction fragment length polymorphism, and variations of these techniques are being developed to improve the sensitivity and specificity of detection and identification processes.

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Figures

Image of Figure 28.1
Figure 28.1

Scanning electron micrographs of oocysts and excysting sporozoites of . (A) Intact oocyst prior to excystation (×11,200). (B) Three sporozoites (Sp) excysting from oocyst simultaneously via the cleaved suture (Su) (×11,200). (C) Empty oocyst (×11,200). (D) Excysted sporozoite (×9,800). Ae, apical end. From Reduker et al. ( ). doi:10.1128/9781555818463.ch28f1

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.2
Figure 28.2

Life cycle of . From Dubey et al. ( ). doi:10.1128/9781555818463.ch28f2

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.3
Figure 28.3

Acid-fast staining. (A) . (B) . (C) . (Bars = 20 μm.) doi:10.1128/9781555818463.ch28f3

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.4
Figure 28.4

oocysts. (A) Phase-contrast microscopy of unsporulated oocysts. (B) Oocysts (OO) in the process of excystation. Note the two sporozoites (SP) free of the sporocyst (SC). (C) Transmission electron microscopy of human small intestine showing intracellular stages. ME, merozoite. doi:10.1128/9781555818463.ch28f4

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.5
Figure 28.5

Bright-field photomicrograph of oocysts unsporulated (A) and sporulated (B). doi:10.1128/9781555818463.ch28f5

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.6
Figure 28.6

Life cycle of . From Dubey and Beattie ( ). doi:10.1128/9781555818463.ch28f6

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.7
Figure 28.7

Transmission electron micrographs of . (A) Sporozoite in parasitophorous vacuole (Pv) of host cell (Hc) at 24 h after inoculation. Am, amylopectin granule; Co, conoid; Mn, microneme; Nu, nucleus of sporozoite; Rh, rhoptry. (B) Final stage of endodyogeny to form two daughter tachyzoites that are still attached to the posterior ends (arrowheads). DG, dense granules; HC, host cell; IT, intravacuolar tubules; M, mitochondrion; MC, microneme; MI, micropore; N, nucleus; RO, rhoptry. From Dubey and Beattie ( ). doi:10.1128/9781555818463.ch28f7

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.8
Figure 28.8

Scanning electron micrograph of trophozoites. One trophozoite shows the dorsal surface and the other shows the ventral surface with sucking disk and flagella. doi:10.1128/9781555818463.ch28f8

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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Image of Figure 28.9
Figure 28.9

Hematoxylin and eosin staining. (A) cyst: (a) with two nuclei and trophozoite; (b) showing two nuclei and median body visible at one pole. (B) cyst: (a) showing large macronucleus and cilia beneath cyst wall and trophozoite; (b) showing oval macronucleus. (C) trophozoite: (a) showing a nucleus and few red blood cells in the cytoplasm and cyst; (b) showing two of four nuclei and rod-shaped inclusion bodies with rounded ends. (Courtesy of Lynne S. Garcia.) doi:10.1128/9781555818463.ch28f9

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28
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References

/content/book/10.1128/9781555818463.chap28
1. Adam, R. D. 2001. Biology of Giardia lamblia. Clin. Microbiol. Rev. 14:447475.
2. Adam, R. D.,, Y. R. Ortega,, R. H. Gilman,, and C. R. Sterling. 2000. Intervening transcribed spacer region 1 variability in Cyclospora cayetanensis. J. Clin. Microbiol. 38:23392343.
3. Ades, A. E. 1991. Evaluating the sensitivity and predictive value of tests of recent infection: toxoplasmosis in pregnancy. Epidemiol. Infect. 107:527535.
4. Adin, A. 2004. Particle count and size alteration for membrane fouling reduction in non-conventional water filtration. Water Sci. Technol. 50:273278.
5. Alarcónde Noya, B.,, Z. Díaz-Bello,, C. Colmenares,, R. Ruiz-Guevara,, L. Mauriello,, R. Zavala-Jaspe,, J. A. Suarez,, T. Abate,, L. Naranjo,, M. Paiva,, L. Rivas,, J. Castro,, J. Márques,, I. Mendoza,, H. Acquatella,, J. Torres,, and O. Noya. 2010. Large urban outbreak of orally acquired acute Chagas disease at a school in Caracas, Venezuela. J. Infect. Dis. 201:13081315.
6. Aldeen, W. E.,, D. Hale,, A. J. Robison,, and K. Carroll. 1995. Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens. Diagn. Microbiol. Infect. Dis. 21:7779.
6a. Alfano-Sobsey, E. M.,, M. L. Eberhard,, J. R. Seed,, D. J. Weber,, K. Y. Won,, E. K. Nace,, and C. L. Moe. 2004. Human challenge pilot study with Cyclospora cayetanensis. Emerg. Infect. Dis. 10:726728.
7. Amos, C. S. 1977. The ocular manifestations of congenital infections produced by Toxoplasma and cytomegalovirus. J. Am. Optom. Assoc. 48:532538.
8. Ang, L. H. 2000. Outbreak of giardiasis in a daycare nursery. Commun. Dis. Public Health 3:212213.
9.Anonymous. 1996. Foodborne outbreak of diarrheal illness associated with Cryptosporidium parvum—Minnesota, 1995. MMWR Morb. Mortal. Wkly. Rep. 45:783784.
10.Anonymous. 1997. Outbreak of cyclosporiasis—northern Virginia-Washington, D.C.-Baltimore, Maryland, Metropolitan area, 1997. Can. Commun. Dis. Rep. 23:166168.
11.Anonymous. 1997. Outbreaks of Escherichia coli O157:H7 infectionand cryptosporidiosis associated with drinking unpasteurized apple cider—Connecticut and New York, October 1996. MMWR Morb. Mortal. Wkly. Rep. 46:48.
12.Anonymous. 1997. Outbreaks of cyclosporiasis—United States, 1997. MMWR Morb. Mortal. Wkly. Rep. 46:451452.
13.Anonymous. 2000. Outbreaks of diarrhea associated with cyanobacteria (blue-green algae)-like-bodies—Chicago and Nepal, 1989 and 1990. MMWR Morb. Mortal. Wkly. Rep. 40:325327.
14.Anonymous. 2004. Outbreak of cyclosporiasis associated with snow peas—Pennsylvania, 2004. MMWR Morb. Mortal. Wkly. Rep. 53:876878.
15. Anusz, K. Z.,, P. H. Mason,, M. W. Riggs,, and L. E. Perryman. 1990. Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay. J. Clin. Microbiol. 28:27702774.
16. Arrowood, M. J.,, and C. R. Sterling. 1989. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J. Clin. Microbiol. 27:14901495.
17. Ashford, R. W. 1979. Occurrence of an undescribed coccidian in man in Papua New Guinea. Ann. Trop. Med. Parasitol. 73:497500.
18. Atwill, E. R.,, K. W. Tate,, M. D. Pereira,, J. Bartolome,, and G. Nader. 2006. Efficacy of natural grassland buffers for removal of Cryptosporidium parvum in rangeland runoff. J. Food Prot. 69:177184.
19. Awadalla, H. N.,, I. F. el Naga,, M. M. el-Temsahi,, and A. Y. Negm. 1998. Detection of Microsporidia by different staining techniques. J. Egypt. Soc. Parasitol. 28:729738.
20. Balatbat, A. B.,, G. W. Jordan,, Y. J. Tang,, and J. Silva, Jr. 1996. Detection of Cryptosporidium parvum DNA in human feces by nested PCR. J. Clin. Microbiol. 34:17691772.
21. Ballal, M.,, T. Prabhu,, A. Chandran,, and P. G. Shivananda. 1999. Cryptosporidium and Isospora belli diarrhoea in immunocompromised hosts. Indian J. Cancer 36:3842.
22. Barcan, L. A.,, M. L. Dallurzo,, L. O. Clara,, A. Valledor,, S. Macias,, E. Zorkin,, S. Gerona,, and B. Livellara. 2002. Toxoplasma gondii pneumonia in liver transplantation: survival after a severe case of reactivation. Transpl. Infect. Dis. 4:9396.
23. Beaugerie, L.,, M. F. Teilhac,, A. M. Deluol,, J. Fritsch,, P. M. Girard,, W. Rozenbaum,, Y. Le Quintrec,, and F. P. Chatelet. 1992. Cholangiopathy associated with Microsporidia infection of the common bile duct mucosa in a patient with HIV infection. Ann. Intern. Med. 117:401402.
24. Berlin, O. G. W.,, L. R. Ash,, C. N. Conteas,, and J. B. Peter. 1999. Rapid, hot chromotrope stain for detecting microsporidia. Clin. Infect. Dis. 29:209.
25. Bern, C.,, B. Hernandez,, M. B. Lopez,, M. J. Arrowood,, M. A. de Mejia,, A. M. de Merida,, A. W. Hightower,, L. Venczel,, B. L. Herwaldt,, and R. E. Klein. 1999. Epidemiologic studies of Cyclospora cayetanensis in Guatemala. Emerg. Infect. Dis. 5:766774.
26. Bicart-See, A.,, P. Massip,, M. D. Linas,, and A. Datry. 2000. Successful treatment with nitazoxanide of Enterocytozoon bieneusi microsporidiosis in a patient with AIDS. Antimicrob. Agents Chemother. 44:167168.
27. Bissuel, F.,, L. Cotte,, M. Rabodonirina,, P. Rougier,, M. A. Piens,, and C. Trepo. 1994. Paromomycin: an effective treatment for cryptosporidial diarrhea in patients with AIDS. Clin. Infect. Dis. 18:447449.
28. Blanshard, C.,, W. S. Hollister,, C. S. Peacock,, D. G. Tovey,, D. S. Ellis,, E. U. Canning,, and B. G. Gazzard. 1992. Simultaneous infection with two types of intestinal microsporidia in a patient with AIDS. Gut 33:418420.
29. Boldorini, R.,, A. Tosoni,, G. Mazzucco,, M. Cernuschi,, P. Caramello,, E. Maran,, G. Costanzi,, and G. Monga. 1996. Intracellular protozoan infection in small intestinal biopsies of patients with AIDS. Light and electron microscopic evaluation. Pathol. Res. Pract. 192:249259.
30. Brady-McCreery, K. M.,, M. A. Hussein,, and E. A. Paysse. 2003. Congenital toxoplasmosis with unusual retinal findings. Arch. Ophthalmol. 121:12001201.
31. Brinkman, K.,, S. Debast,, R. Sauerwein,, F. Ooyman,, J. Hiel,, and J. Raemaekers. 1998. Toxoplasma retinitis/encephalitis 9 months after allogeneic bone marrow transplantation. Bone Marrow Transplant. 21:635636.
32. Cabada, M. M.,, and A. C. White, Jr. 2010. Treatment of cryptosporidiosis: do we know what we think we know Curr. Opin. Infect. Dis. 23:494499.
33. Caccio, S.,, E. Pinter,, R. Fantini,, I. Mezzaroma,, and E. Pozio. 2002. Human infection with Cryptosporidium felis: case report and literature review. Emerg. Infect. Dis. 8:8586.
34. Cali, A.,, L. Weiss,, P. Takvorian,, H. Tanowitz,, and M. Wittner. 1994. Ultrastructural identification of AIDS associated microsporidiosis. J. Eukaryot. Microbiol. 41:24S.
35. Cama, V. A.,, and C. R. Sterling. 1991. Hyperimmune hens as a novel source of anti-Cryptosporidium antibodies suitable for passive immune transfer. J. Protozool. 38:42S43S.
36. Carreno, R. A.,, D. S. Martin,, and J. R. Barta. 1999. Cryptosporidium is more closely related to the gregarines than to coccidia as shown by phylogenetic analysis of apicomplexan parasites inferred using small-subunit ribosomal RNA gene sequences. Parasitol. Res. 85:899904.
37. Casemore, D. P.,, M. Armstrong,, and R. L. Sands. 1985. Laboratory diagnosis of cryptosporidiosis. J. Clin. Pathol. 38:13371341.
38. Chauret, C. P.,, C. Z. Radziminski,, M. Lepuil,, R. Creason,, and R. C. Andrews. 2001. Chlorine dioxide inactivation of Cryptosporidium parvum oocysts and bacterial spore indicators. Appl. Environ. Microbiol. 67:29933001.
39. Chu, D. M.,, J. B. Sherchand,, J. H. Cross,, and P. A. Orlandi. 2004. Detection of Cyclospora cayetanensis in animal fecal isolates from Nepal using an FTA filter-base polymerase chain reaction method. Am. J. Trop. Med. Hyg. 71:373379.
40. Chui, D. W.,, and R. L. Owen. 1994. AIDS and the gut. J. Gastroenterol. Hepatol. 9:291303.
41. Comin, C. E.,, and M. Santucci. 1994. Submicroscopic profile of Isospora belli enteritis in a patient with acquired immune deficiency syndrome. Ultrastruct. Pathol. 18:473482.
42. Connor, B. A.,, J. Reidy,, and R. Soave. 1999. Cyclosporiasis: clinical and histopathologic correlates. Clin. Infect. Dis. 28:12161222.
43. Conrath, J.,, A. Mouly-Bandini,, F. Collart,, and B. Ridings. 2003. Toxoplasma gondii retinochoroiditis after cardiac transplantation. Graefes Arch. Clin. Exp. Ophthalmol. 241:334338.
44. Costa, S. F.,, and L. M. Weiss. 2000. Drug treatment of microsporidiosis. Drug Resist. Updat. 3:384399.
45. Coupe, S.,, C. Sarfati,, S. Hamane,, and F. Derouin. 2005. Detection of Cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. J. Clin. Microbiol. 43:10171023.
46. Current, W. L.,, and P. H. Bick. 1989. Immunobiology of Cryptosporidium spp. Pathol. Immunopathol. Res. 8:141160.
47. Curry, A. 2003. Electron microscopy and the investigation of new infectious diseases. Int. J. Infect. Dis. 7:251257.
48. Danziger, L. H.,, T. P. Kanyok,, and R. M. Novak. 1993. Treatment of cryptosporidial diarrhea in an AIDS patient with paromomycin. Ann. Pharmacother. 27:14601462.
49. DeHovitz, J. A.,, J. W. Pape,, M. Boncy,, and W. D. Johnson, Jr. 1986. Clinical manifestations and therapy of Isospora belli infection in patients with the acquired immunodeficiency syndrome. N. Engl. J. Med. 315:8790.
50. De Marco, R.,, R. Ceccarelli,, R. Frulio,, C. Palmero,, and P. Vittone. 2003. Retinochoroiditis associated with congenital toxoplasmosis in children: IgG antibody profiles demonstrating the synthesis of local antibodies. Eur. J. Ophthalmol. 13:7479.
51. Deng, M. Q.,, and D. O. Cliver. 2000. Comparative detection of Cryptosporidium parvum oocysts from apple juice. Int. J. Food Microbiol. 54:155162.
52. Derouin, F. 1992. Pathogeny and immunological control of toxoplasmosis. Braz. J. Med. Biol. Res. 25:11631169.
53. Derouin, F.,, A. Debure,, E. Godeaut,, M. Lariviere,, and H. Kreis. 1987. Toxoplasma antibody titers in renal transplant recipients. Pretransplant evaluation and posttransplant follow-up of 73 patients. Transplantation 44:515518.
54. Diaz, J. H. 2007. Chagas disease in the United States: a cause for concern in Louisiana J. La. State Med. Soc. 159:2129.
55. Ditrich, O.,, L. Palkovic,, J. Sterba,, J. Prokopic,, J. Loudová,, and M. Giboda. 1991. The first finding of Cryptosporidium baileyi in man. Parasitol. Res. 77:4447.
56. Doller, P. C.,, K. Dietrich,, N. Filipp,, S. Brockmann,, C. Dreweck,, R. Vonthein,, C. Wagner-Wiening,, and A. Wiedenmann. 2002. Cyclosporiasis outbreak in Germany associated with the consumption of salad. Emerg. Infect. Dis. 8:992994.
57. Dubey, J. P.,, and C. P. Beattie. 1988. Toxoplasmosis of Animals and Man. CRC Press, Inc., Boca Raton, FL.
58. Dubey, J. P.,, N. L. Miller,, and J. K. Frenkel. 1970. Toxoplasma gondii life cycle in cats. J. Am. Vet. Med. Assoc. 157:17671770.
59. Dubey, J. P.,, D. W. Thayer,, C. A. Speer,, and S. K. Shen. 1998. Effect of gamma irradiation on unsporulated and sporulated Toxoplasma gondii oocysts. Int. J. Parasitol. 28:369375.
60. Eberhard, M. L.,, A. J. da Silva,, B. G. Lilley,, and N. J. Pieniazek. 1999. Morphologic and molecular characterization of new Cyclospora species from Ethiopian monkeys: C. cercopitheci sp.n., C. colobi sp.n., and C. papionis sp.n. Emerg. Infect. Dis. 5:651658.
61. Eberhard, M. L.,, Y. R. Ortega,, D. E. Hanes,, E. K. Nace,, R. Q. Do,, M. G. Robl,, K. Y. Won,, C. Gavidia,, N. L. Sass,, K. Mansfield,, A. Gozalo,, J. Griffiths,, R. Gilman,, C. R. Sterling,, and M. J. Arrowood. 2000. Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals. J. Parasitol. 86:577582.
62. Erlandsen, S. L.,, and W. J. Bemrick. 1987. SEM evidence for a new species, Giardia psittaci. J. Parasitol. 73:623629.
63. Fayer, R. 2004. Sarcocystis spp. in human infections. Clin. Microbiol. Rev. 17:894902.
64. Fayer, R.,, C. A. Spear,, and J. P. Dubey,. 1990. The general biology of Cryptosporidium, p. 141. In R. Fayer (ed.), Cryptosporidium and Cryptosporidiosis. CRC Press, Boca Raton, FL.
65. Fayer, R.,, M. Tilley,, S. J. Upton,, A. J. Guidry,, D. W. Thayer,, M. Hildreth,, and J. Thomson. 1991. Production and preparation of hyperimmune bovine colostrum for passive immunotherapy of cryptosporidiosis. J. Protozool. 38:38S39S.
66. Fayer, R.,, J. M. Trout,, E. J. Lewis,, M. Santin,, L. Zhou,, A. A. Lal,, and L. Xiao. 2003. Contamination of Atlantic coast commercial shellfish with Cryptosporidium. Parasitol. Res. 89:141145.
67. Fayer, R.,, J. M. Trout,, L. Xiao,, U. M. Morgan,, A. A. Lai,, and J. P. Dubey. 2001. Cryptosporidium canis n. sp. from domestic dogs. J. Parasitol. 87:14151422.
68. Fedorko, D. P.,, N. A. Nelson,, and C. P. Cartwright. 1995. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. J. Clin. Microbiol. 33:17391741.
69. Finch, G. R.,, E. K. Black,, C. W. Labatiuk,, L. Gyürék,, and M. Belosevic. 1993. Comparison of Giardia lamblia and Giardia muris cyst inactivation by ozone. Appl. Environ. Microbiol. 59:36743680.
70. Flanagan, P. A. 1992. Giardia—diagnosis, clinical course and epidemiology. A review. Epidemiol. Infect. 109:122.
71. Fleming, C. A.,, D. Caron,, J. E. Gunn,, and M. A. Barry. 1998. A foodborne outbreak of Cyclospora cayetanensis at a wedding: clinical features and risk factors for illness. Arch. Intern. Med. 158:11211125.
72. Furio, M. M.,, and C. J. Wordell. 1985. Treatment of infectious complications of acquired immunodeficiency syndrome. Clin. Pharm. 4:539554.
73. Gallant, J. E.,, R. D. Moore,, and R. E. Chaisson. 1994. Prophylaxis for opportunistic infections in patients with HIV infection. Ann. Intern. Med. 120:932944.
74. Galvagno, G.,, G. Cattaneo,, and E. Reverso-Giovantin. 1993. Chronic diarrhea due to Cryptosporidium: the efficacy of spiramycin treatment. Pediatr. Med. Chir. 15:297298. (In Italian.)
75. Garcia, L. S. 2007. Intestinal protozoa: flagellates and ciliates, p. 3148. In Diagnostic Medical Parasitology, 5th ed. American Society for Microbiology, Washington, DC.
76. Garcia, L. S. 2007. Intestinal protozoa: amebae, p. 630. In Diagnostic Medical Parasitology, 5th ed. American Society for Microbiology, Washington, DC.
77. Garcia-López, H. L.,, L. E. Rodríguez-Tovar,, and C. E. Medina-De la Garza. 1996. Identification of Cyclospora in poultry. Emerg. Infect. Dis. 2:356357.
78. Gatei, W.,, R. W. Ashford,, N. J. Beeching,, S. K. Kamwati,, J. Greensill,, and C. A. Hart. 2002. Cryptosporidium muris infection in an HIV-infected adult, Kenya. Emerg. Infect. Dis. 8:204206.
79. Giacometti, A.,, F. Burzacchini,, O. Cirioni,, F. Barchiesi,, M. Dini,, and G. Scalise. 1999. Efficacy of treatment with paromomycin, azithromycin, and nitazoxanide in a patient with disseminated cryptosporidiosis. Eur. J. Clin. Microbiol. Infect. Dis. 18:885889.
80. Gomez-Bautista, M.,, L. M. Ortega-Mora,, E. Tabares,, V. Lopez-Rodas,, and E. Costas. 2000. Detection of infectious Cryptosporidium parvum oocysts in mussels (Mytilus galloprovincialis) and cockles (Cerastoderma edule). Appl. Environ. Microbiol. 66:18661870.
81. Gómez-Couso, H.,, F. Freire-Santos,, C. F. Amar,, K. A. Grant,, K. Williamson,, M. E. Ares-Mazás,, and J. McLauchlin. 2004. Detection of Cryptosporidium and Giardia in molluscan shellfish by multiplexed nested-PCR. Int. J. Food Microbiol. 91:279288.
82. Gómez-Couso, H.,, F. Freire-Santos,, G. A. Hernández-Córdova,, and M. E. Ares-Mazás. 2005. A histological study of the transit of Cryptosporidium parvum oocysts through clams (Tapes decussatus). Int. J. Food Microbiol. 102:5762.
83. Gómez-Couso, H.,, F. Freire-Santos,, J. Martínez-Urtaza,, O. García-Martín,, and M. E. Ares-Mazás. 2003. Contamination of bivalve molluscs by Cryptosporidium oocysts: the need for new quality control standards. Int. J. Food Microbiol. 87:97105.
84. Gómez-Couso, H.,, F. Freire-Santos,, M. R. Ortega-Iñarrea,, J. A. Castro-Hermida,, and M. E. Ares-Mazás. 2003. Environmental dispersal of Cryptosporidium parvum oocysts and cross transmission in cultured bivalve molluscs. Parasitol. Res. 90:140142.
85. Gómez-Couso, H.,, F. Méndez-Hermida,, J. A. Castro-Hermida,, and E. Ares-Mazás. 2006. Cryptosporidium contamination in harvesting areas of bivalve molluscs. J. Food Prot. 69:185190.
86. Gómez-Couso, H.,, F. Méndez-Hermida,, J. A. Castro-Hermida,, and E. Ares-Mazás. 2005. Giardia in shellfish-farming areas: detection in mussels, river water and waste waters. Vet. Parasitol. 133:1318.
87. Graczyk, T. K.,, and K. J. Schwab. 2000. Foodborne infections vectored by molluscan shellfish. Curr. Gastroenterol. Rep. 2:305309.
88. Guerreiro, N. M.,, P. M. Herrera,, L. de Escalona,, C. E. de Kolster,, V. G. de Yanes,, O. de Febres,, O. Naveda,, and M. de Naveda. 1991. Giardia lamblia: comparison of two diagnostic methods and evaluation of response to treatment with metronidazole. G. E. N. 45:105110. (In Spanish.)
89. Hayes, E. B.,, T. D. Matte,, T. R. O’Brien,, T. W. McKinley,, G. S. Logsdon,, J. B. Rose,, B. L. Ungar,, D. M. Word,, M. A. Wilson,, E. G. Long,, E. S. Hurwitz,, and D. D. Juranek. 1989. Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply. N. Engl. J. Med. 320:13721376.
90. Herwaldt, B. L. 2000. Cyclospora cayetanensis: a review, focusing on the outbreaks of cyclosporiasis in the 1990s. Clin. Infect. Dis. 31:10401057.
91. Herwaldt, B. L.,, M. L. Ackers, and the Cyclospora Working Group. 1997. An outbreak in 1996 of cyclosporiasis associated with imported raspberries. N. Engl. J. Med. 336:15481556.
92. Herwaldt, B. L.,, M. J. Beach, and the Cyclospora Working Group. 1999. The return of Cyclospora in 1997: another outbreak of cyclosporiasis in North America associated with imported raspberries. Ann. Intern. Med. 130:210220.
93. Hijjawi, N. S.,, B. P. Meloni,, U. M. Ryan,, M. E. Olson,, and R. C. Thompson. 2002. Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium. Int. J. Parasitol. 32:17191726.
94. Hill, D. R. 1993. Giardiasis. Issues in diagnosis and management. Infect. Dis. Clin. North Am. 7:503525.
95. Ho, A. Y.,, A. S. Lopez,, M. G. Eberhart,, R. Levenson,, B. S. Finkel,, A. J. da Silva,, J. M. Roberts,, P. A. Orlandi,, C. C. Johnson,, and B. L. Herwaldt. 2002. Outbreak of cyclosporiasis associated with imported raspberries, Philadelphia, Pennsylvania, 2000. Emerg. Infect. Dis. 8:783788.
96. Hoang, L. M.,, M. Fyfe,, C. Ong,, J. Harb,, S. Champagne,, B. Dixon,, and J. Isaac-Renton. 2005. Outbreak of cyclosporiasis in British Columbia associated with imported Thai basil. Epidemiol. Infect. 133:2327.
97. Huang, P.,, J. T. Weber,, D. M. Sosin,, P. M. Griffin,, E. G. Long,, J. J. Murphy,, F. Kocka,, C. Peters,, and C. Kallick. 1995. The first reported outbreak of diarrheal illness associated with Cyclospora in the United States. Ann. Intern. Med. 123:409414.
98. Hume, O. S. 1972. Toxoplasmosis and pregnancy. Am. J. Obstet. Gynecol. 114:703715.
99. Insulander, M.,, B. Svenungsson,, M. Lebbad,, L. Karlsson,, and B. de Jong. 2010. A foodborne outbreak of Cyclospora infection in Stockholm, Sweden. Foodborne Pathog. Dis. 7:15851587.
100. Jinneman, K. C.,, J. H. Wetherington,, W. E. Hill,, A. M. Adams,, J. M. Johnson,, B. J. Tenge,, N. L. Dang,, R. L. Manger,, and M. M. Wekell. 1998. Template preparation for PCR and RFLP of amplification products for the detection and identification of Cyclospora sp. and Eimeria spp. oocysts directly from raspberries. J. Food Prot. 61:14971503.
101. Jinneman, K. C.,, J. H. Wetherington,, W. E. Hill,, C. J. Omiescinski,, A. M. Adams,, J. M. Johnson,, B. J. Tenge,, N. L. Dang,, and M. M. Wekell. 1999. An oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products. J. Food Prot. 62:682685.
102. Karasudani, T.,, S. Aoki,, J. Takeuchi,, M. Okuyama,, M. Oseto,, S. Matsuura,, T. Asai,, and H. Inouye. 2001. Sensitive detection of Cryptosporidium oocysts in environmental water samples by reverse transcription-PCR. Jpn. J. Infect. Dis. 54:122124.
103. Kniel, K. E.,, and M. C. Jenkins. 2005. Detection of Cryptosporidium parvum oocysts on fresh vegetables and herbs using antibodies specific for a Cryptosporidium parvum viral antigen. J. Food Prot. 68:10931096.
104. Kong, L. I.,, L. J. Swango,, B. L. Blagburn,, C. M. Hendrix,, D. E. Williams,, and S. D. Worley. 1988. Inactivation of Giardia lamblia and Giardia canis cysts by combined and free chlorine. Appl. Environ. Microbiol. 54:25802582.
105. Korich, D. G.,, J. R. Mead,, M. S. Madore,, N. A. Sinclair,, and C. R. Sterling. 1990. Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability. Appl. Environ. Microbiol. 56:14231428.
106. Laberge, I.,, M. W. Griffiths,, and M. W. Griffiths. 1996. Prevalence, detection and control of Cryptosporidium parvum in food. Int. J. Food Microbiol. 32:126.
107. Lanfredi-Rangel, A.,, J. A. Diniz, Jr.,, and W. de Souza. 1999. Presence of a protrusion on the ventral disk of adhered trophozoites of Giardia lamblia. Parasitol. Res. 85:951955.
108. Long, E. G.,, A. Ebrahimzadeh,, E. H. White,, B. Swisher,, and C. S. Callaway. 1990. Alga associated with diarrhea in patients with acquired immunodeficiency syndrome and in travelers. J. Clin. Microbiol. 28:11011104.
109. Long, E. G.,, E. H. White,, W. W. Carmichael,, P. M. Quinlisk,, R. Raja,, B. L. Swisher,, H. Daugharty,, and M. T. Cohen. 1991. Morphologic and staining characteristics of a cyanobacterium-like organism associated with diarrhea. J. Infect. Dis. 164:199202.
110. Lopez, A.S.,, D. R. Dodson,, M. J. Arrowood,, P. A. Orlandi, Jr.,, A.J. da Silva,, J.W. Bier,, S.D. Hanauer,, R.L. Kuster,, S. Oltman,, M. S. Baldwin,, K. Y. Won,, E. M. Nace,, M. L. Eberhard,, and B. L. Herwaldt. 2001. Outbreak of cyclosporiasis associated with basil in Missouri in 1999. Clin. Infect. Dis. 32:10101017.
111. Lopez, C. E.,, A. C. Dykes,, D. D. Juranek,, S. P. Sinclair,, J. M. Conn,, R. W. Christie,, E. C. Lippy,, M. G. Schultz,, and M. H. Mires. 1980. Waterborne giardiasis: a communitywide outbreak of disease and a high rate of asymptomatic infection. Am. J. Epidemiol. 112:495507.
112. Lopez, F. A.,, J. Manglicmot,, T. M. Schmidt,, C. Yeh,, H. V. Smith,, and D. A. Relman. 1999. Molecular characterization of Cyclospora-like organisms from baboons. J. Infect. Dis. 179:670676.
113. López-Vélez, R.,, R. Tarazona,, C. A. Garcia,, E. Gomez-Mampaso,, A. Guerrero,, V. Moreira,, and R. Villanueva. 1995. Intestinal and extraintestinal cryptosporidiosis in AIDS patients. Eur. J. Clin. Microbiol. Infect. Dis. 14:677681.
114. Luna, V. A.,, B. K. Stewart,, D. L. Bergeron,, C. R. Clausen,, J. J. Plorde,, and T. R. Fritsche. 1995. Use of the fluorochrome calcofluor white in the screening of stool specimens for spores of microsporidia. Am. J. Clin. Pathol. 103:656659.
115. Lunden, A.,, and A. Uggla. 1992. Infectivity of Toxoplasma gondii in mutton following curing, smoking, freezing or microwave cooking. Int. J. Food Microbiol. 15:357363.
116. Mac Kenzie, W. R.,, N. J. Hoxie,, M. E. Proctor,, M. S. Gradus,, K. A. Blair,, D. E. Peterson,, J. J. Kazmierczak,, D. G. Addiss,, K. R. Fox,, J. B. Rose,, and J. P. Davis. 1994. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. N. Engl. J. Med. 331:161167.
117. MacRae, M.,, C. Hamilton,, N. J. Strachan,, S. Wright,, and I. D. Ogden. 2005. The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish. J. Microbiol. Methods 60:395401.
118. Madico, G.,, J. McDonald,, R. H. Gilman,, L. Cabrera,, and C. R. Sterling. 1997. Epidemiology and treatment of Cyclospora cayetanensis infection in Peruvian children. Clin. Infect. Dis. 24:977981.
119. Marshall, M. M.,, D. Naumovitz,, Y. Ortega,, and C. R. Sterling. 1997. Waterborne protozoan pathogens. Clin. Microbiol. Rev. 10:6785.
120. Matos, O.,, M. Alves,, L. Xiao,, V. Cama,, and F. Antunes. 2004. Cryptosporidium felis and C. meleagridis in persons with HIV, Portugal. Emerg. Infect. Dis. 10:22562257.
121. Miles, M. A. 2010. Orally acquired Chagas disease: lessons from an urban school outbreak. J. Infect. Dis. 201:12821284.
122. Millard, P. S.,, K. F. Gensheimer,, D. G. Addiss,, D. M. Sosin,, G. A. Beckett,, A. Houck-Jankoski,, and A. Hudson. 1994. An outbreak of cryptosporidiosis from fresh-pressed apple cider. JAMA 272:15921596.
123. Monis, P. T.,, R. H. Andrews,, G. Mayrhofer,, and P. L. Ey. 2003. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect. Genet. Evol. 3:2938.
124. Morgan-Ryan, U. M.,, A. Fall,, L. A. Ward,, N. Hijjawi,, I. Sulaiman,, R. Fayer,, R. C. Thompson,, M. Olson,, A. Lal,, and L. Xiao. 2002. Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. J. Eukaryot. Microbiol. 49:433440.
125. Moskovitz, B.L.,, T. L. Stanton,, and J. J. Kusmierek. 1988. Spiramycin therapy forcryptosporidial diarrhoea in immunocompromised patients. J. Antimicrob.Chemother. 22(Suppl. B):189191.
126. Müller, A.,, K. Stellermann,, P. Hartmann,, M. Schrappe,, G. Fätkenheuer,, B. Salzberger,, V. Diehl,, and C. Franzen. 1999. A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens. Clin. Diagn. Lab. Immunol. 6:243246.
127. Nóbrega, A. A.,, M. H. Garcia,, E. Tatto,, M. T. Obara,, E. Costa,, J. Sobel,, and W. N. Araujo. 2009. Oral transmission of Chagas disease by consumption of açaí palm fruit, Brazil. Emerg. Infect. Dis. 15:653655.
128. Olivier, C.,, S. van de Pas,, P. W. Lepp,, K. Yoder,, and D. A. Relman. 2001. Sequence variability in the first internal transcribed spacer region within and among Cyclospora species is consistent with polyparasitism. Int. J. Parasitol. 31:14751487.
129. Orlandi, P. A.,, D. M. Chu,, J. W. Bier,, and G. J. Jackson. 2002. Parasites in the food supply. Food Technol. 56:72.
130. Orlandi, P. A.,, and K. A. Lampel. 2000. Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J. Clin. Microbiol. 38:22712277.
131. Ortega, Y. R.,, R. H. Gilman,, and C. R. Sterling. 1994. A new coccidian parasite (Apicomplexa: Eimeriidae) from humans. J. Parasitol. 80:625629.
132. Ortega, Y. R.,, R. Nagle,, R. H. Gilman,, J. Watanabe,, J. Miyagui,, H. Quispe,, P. Kanagusuku,, C. Roxas,, and C. R. Sterling. 1997. Pathologic and clinical findings in patients with cyclosporiasis and a description of intracellular parasite life-cycle stages. J. Infect. Dis. 176:15841589.
133. Ortega, Y. R.,, C. R. Roxas,, R. H. Gilman,, N. J. Miller,, L. Cabrera,, C. Taquiri,, and C. R. Sterling. 1997. Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region in Peru. Am. J. Trop. Med. Hyg. 57:683686.
134. Ortega, Y. R.,, C. R. Sterling,, R. H. Gilman,, V. A. Cama,, and F. Diaz. 1993. Cyclospora species—a new protozoan pathogen of humans. N. Engl. J. Med. 328:13081312.
135. Pape, J. W.,, R. I. Verdier,, M. Boncy,, J. Boncy,, and W. D. Johnson, Jr. 1994. Cyclospora infection in adults infected with HIV. Clinical manifestations, treatment, and prophylaxis. Ann. Intern. Med. 121:654657.
136. Payment, P. 1999. Poor efficacy of residual chlorine disinfectant in drinking water to inactivate waterborne pathogens in distribution systems. Can. J. Microbiol. 45:709715.
137. Pedraza-Díaz, S.,, C. F. Amar,, J. McLauchlin,, G. L. Nichols,, K. M. Cotton,, P. Godwin,, A. M. Iversen,, L. Milne,, J. R. Mulla,, K. Nye,, H. Panigrahl,, S. R. Venn,, R. Wiggins,, M. Williams,, and E. R. Youngs. 2001. Cryptosporidium meleagridis from humans: molecular analysis and description of affected patients. J. Infect. 42:243250.
138. Peng, M. M.,, L. Xiao,, A. R. Freeman,, M. J. Arrowood,, A. A. Escalante,, A. C. Weltman,, C. S. Ong,, W. R. Mac Kenzie,, A. A. Lal,, and C. B. Beard. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerg. Infect. Dis. 3:567573.
139. Perryman, L.E.,, and J. M. Bjorneby. 1991. Immunotherapy ofcryptosporidiosis in immunodeficient animal models. J. Protozool. 38:98S100S.
140. Pönka, A.,, H. Kotilainen,, R. Rimhanen-Finne,, P. Hokkanen,, M. L. Hänninen,, A. Kaarna,, T. Meri,, and M. Kuusi. 2009. A foodborne outbreak due to Cryptosporidium parvum in Helsinki, November 2008. Euro Surveill. 14:pii=19269.
141. Portnoy, D.,, M. E. Whiteside,, E. Buckley III,, and C. L. MacLeod. 1984. Treatment of intestinal cryptosporidiosis with spiramycin. Ann. Intern. Med. 101:202204.
142. Posada, G.,, D. Pizarro,, and E. Mohs. 1987. Oral rehydration in children with Cryptosporidium muris diarrhea. Bol. Med. Hosp. Infant. Mex. 44:740744. (In Spanish.)
143. Quiroz, E. S.,, C. Bern,, J. R. MacArthur,, L. Xiao,, M. Fletcher,, M. J. Arrowood,, D. K. Shay,, M. E. Levy,, R. I. Glass,, and A. Lal. 2000. An outbreak of cryptosporidiosis linked to a foodhandler. J. Infect. Dis. 181:695700.
144. Rabold, J. G.,, C. W. Hoge,, D. R. Shlim,, C. Kefford,, R. Rajah,, and P. Echeverria. 1994. Cyclospora outbreak associated with chlorinated drinking water. Lancet 344:13601361.
145. Radziminski, C.,, L. Ballantyne,, J. Hodson,, R. Creason,, R. C. Andrews,, and C. Chauret. 2002. Disinfection of Bacillus subtilis spores with chlorine dioxide: a bench-scale and pilot-scale study. Water Res. 36:16291639.
146. Raisanen, S. 1978. Toxoplasmosis transmitted by blood transfusions. Transfusion 18:329332.
147. Reduker, D. W.,, C. A. Speer,, and J. A. Blixt. 1985. Ultrastructure of Cryptosporidium parvum oocysts and excysting sporozoites as revealed by high resolution scanning electron microscopy. J. Protozool. 32:708711.
148. Reiter-Owona, I.,, H. Seitz,, U. Gross,, M. Sahm,, J. K. Rockstroh,, and H. M. Seitz. 2000. Is stage conversion the initiating event for reactivation of Toxoplasma gondii in brain tissue of AIDS patients J. Parasitol. 86:531536.
149. Rice, E. W.,, and J. C. Hoff. 1981. Inactivation of Giardia lamblia cysts by ultraviolet irradiation. Appl. Environ. Microbiol. 42:546547.
150. Ripabelli, G.,, A. Leone,, M. L. Sammarco,, I. Fanelli,, G. M. Grasso,, and J. McLauchlin. 2004. Detection of Cryptosporidium parvum oocysts in experimentally contaminated lettuce using filtration, immunomagnetic separation, light microscopy, and PCR. Foodborne Pathog. Dis. 1:216222.
151. Robertson, L. J.,, and B. Gjerde. 2001. Occurrence of parasites on fruits and vegetables in Norway. J. Food Prot. 64:17931798.
152. Robertson, L. J.,, J. D. Greig,, B. Gjerde,, and A. Fazil. 2005. The potential for acquiring cryptosporidiosis or giardiosis from consumption of mung bean sprouts in Norway: a preliminary step-wise risk assessment. Int. J. Food Microbiol. 98:291300.
153. Rosales, M. J.,, G. P. Cordón,, M. S. Moreno,, and C. M. Sánchez. 2005. Extracellular like-gregarine stages of Cryptosporidium parvum. Acta Trop. 95:7478.
154. Rose, J. B.,, C. N. Haas,, and S. Regli. 1991. Risk assessment and control of waterborne giardiasis. Am. J. Public Health 81:709713.
155. Sáez-Llorens, X.,, C. M. Odio,, M. A. Umaña,, and M. V. Morales. 1989. Spiramycin vs. placebo for treatment of acute diarrhea caused by Cryptosporidium. Pediatr. Infect. Dis. J. 8:136140.
156. Scallan, E.,, R. M. Hoekstra,, F. J. Angulo,, R. V. Tauxe,, M. A. Widdowson,, S. L. Roy,, J. L. Jones,, and P. M. Griffin. 2011. Foodborne illness acquired in the United States—major pathogens. Emerg. Infect. Dis. 17:715.
157. Schottelius, J.,, E. M. Kuhn,, and R. Enriquez. 2000. Microsporidia and Candida spores: their discrimination by Calcofluor, trichrome-blue and methylene-blue combination staining. Trop. Med. Int. Health 5:453458.
158. Sepulveda, B. 1982. Amebiasis: host-pathogen biology. Rev Infect. Dis. 4:12471253.
159. Sherchand, J. B.,, J. H. Cross,, M. Jimba,, S. Sherchand,, and M. P. Shrestha. 1999. Study of Cyclospora cayetanensis in health care facilities, sewage water and green leafy vegetables in Nepal. Southeast Asian J. Trop. Med. Public Health 30:5863.
160. Shields, J. M.,, and B. H. Olson. 2003. PCR-restriction fragment length polymorphism method for detection of Cyclospora cayetanensis in environmental waters without microscopic confirmation. Appl. Environ. Microbiol. 69:46624669.
161. Shlim, D. R.,, M. T. Cohen,, M. Eaton,, R. Rajah,, E. G. Long,, and B. L. Ungar. 1991. An alga-like organism associated with an outbreak of prolonged diarrhea among foreigners in Nepal. Am. J. Trop. Med. Hyg. 45:383389.
162. Shlim, D. R.,, C. W. Hoge,, R. Rajah,, R. M. Scott,, P. Pandy,, and P. Echeverria. 1999. Persistent high risk of diarrhea among foreigners in Nepal during the first 2 years of residence. Clin. Infect. Dis. 29:613616.
163. Singh, K. D.,, D. K. Bhasin,, S. V. Rana,, K. Vaiphei,, R. Katyal,, V. K. Vinayak,, and K. Singh. 2000. Effect of Giardia lamblia on duodenal disaccharidase levels in humans. Trop. Gastroenterol. 21:174176.
164. Sironi, M.,, C. Bandi,, S. Novati,, and M. Scaglia. 1997. A PCR-RFLP method for the detection and species identification of human microsporidia. Parassitologia 39:437439.
165. Smith, H. V.,, C. A. Paton,, R. W. Girdwood,, and M. M. Mtambo. 1996. Cyclospora in non-human primates in Gombe, Tanzania. Vet. Rec. 138:528.
166. Soave, R. 1988. Cryptosporidiosis and isosporiasis in patients with AIDS. Infect. Dis. Clin. North Am. 2:485493.
167. Speelman, P. 1985. Single-dose tinidazole for the treatment of giardiasis. Antimicrob. Agents Chemother. 27:227229.
168. Sun, T.,, C. F. Ilardi,, D. Asnis,, A. R. Bresciani,, S. Goldenberg,, B. Roberts,, and S. Teichberg. 1996. Light and electron microscopic identification of Cyclospora species in the small intestine. Evidence of the presence of asexual life cycle in human host. Am. J. Clin. Pathol. 105:216220.
169. Tee, G. H.,, A. H. Moody,, A. H. Cooke,, and P. L. Chiodini. 1993. Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces. J. Clin. Pathol. 46:555558.
170. Ungar, B. L. 1990. Enzyme-linked immunoassay for detection of Cryptosporidium antigens in fecal specimens. J. Clin. Microbiol. 28:24912495.
171. van Keulen, H.,, S. R. Campbell,, S. L. Erlandsen,, and E. L. Jarroll. 1991. Cloning and restriction enzyme mapping of ribosomal DNA of Giardia duodenalis, Giardia ardeae and Giardia muris. Mol. Biochem. Parasitol. 46:275284.
172. Verdier, R. I.,, D. W. Fitzgerald,, W. D. Johnson, Jr.,, and J. W. Pape. 2000. Trimethoprim-sulfamethoxazole compared with ciprofloxacin for treatment and prophylaxis of Isospora belli and Cyclospora cayetanensis infection in HIV-infected patients. A randomized, controlled trial. Ann. Intern. Med. 132:885888.
173. von Gunten, U. 2003. Ozonation of drinking water: part II. Disinfection and by-product formation in presence of bromide, iodide or chlorine. Water Res. 37:14691487.
174. Xiao, L. 2010. Molecular epidemiology of cryptosporidiosis: an update. Exp. Parasitol. 124:8089.
175. Yai, L. E.,, A. R. Bauab,, M. P. Hirschfeld,, M. L. de Oliveira,, and J. T. Damaceno. 1997. The first two cases of Cyclospora in dogs, Sao Paulo, Brazil. Rev. Inst. Med. Trop. Sao Paulo 39:177179.
176. Zerpa, R.,, N. Uchima,, and L. Huicho. 1995. Cyclospora cayetanensis associated with watery diarrhoea in Peruvian patients. J. Trop. Med. Hyg. 98:325329.
177. Zimmerman, S.K.,, and C. A. Needham. 1995. Comparison ofconventional stool concentration and preserved-smear methods with Merifluor Cryptosporidium/Giardia Direct Immunofluorescence Assay andProSpecT Giardia EZ Microplate Assay for detection of Giardialamblia. J. Clin. Microbiol. 33:19421943.
178. Zu, S. X.,, and R. L. Guerrant. 1993. Cryptosporidiosis. J. Trop. Pediatr. 39:132136.

Tables

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Table 28.1

Protozoa of medical importance acquired from food and water

Citation: Ortega Y. 2013. Protozoan Parasites, p 713-733. In Doyle M, Buchanan R (ed), Food Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555818463.ch28

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