1887

Chapter 21 : Darwin in My Lab

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $7.00

Preview this chapter:
Zoom in
Zoomout

Darwin in My Lab, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818470/9781555815400_Chap21-1.gif /docserver/preview/fulltext/10.1128/9781555818470/9781555815400_Chap21-2.gif

Abstract:

The author’s methods for direct visualization of individual mutation and recombination events in living bacteria are based only on the shrewd use of a natural DNA modification (methylation of A in the GATC sequences) and of two fluorescent proteins. A special protein involved in the process of DNA error correction (called mismatch repair) binds exclusively, extensively, and stably to uncorrected DNA copy errors, forming a fluorescent focus in the living cell. In recombination, a fluorescent version of the SeqA protein, which binds exclusively the hemimethylated DNA (a DNA duplex with only one strand methylated), allows us to monitor the integrity of permanently hemimethylated DNA over an unlimited number of generations. The molecular phylogenies of preexisting mutations from genomes of many sequenced natural isolates of suggest that the acquisition of a mutation at any locus in the genome is about 100 times more likely to occur by recombination than by de novo mutation. The results of a study conducted by the author, appeared as expected from the serovar Typhimurium crosses: when both partners had mutator histories, genetic recombination dropped 10-fold in cells but not in cells with defects; when only one partner had mutator history, recombination dropped only 3- to 4-fold; but when neither had a mutator history, there was no effect on recombination.

Citation: Radman M. 2012. Darwin in My Lab, p 151-157. In Kolter R, Maloy S (ed), Microbes and Evolution. ASM Press, Washington, DC. doi: 10.1128/9781555818470.ch21

Key Concept Ranking

Genetic Recombination
0.5714492
Horizontal Gene Transfer
0.43351683
0.5714492
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555818470.chap21

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error