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Chapter 46 : Laboratory Diagnosis of Syphilis

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Laboratory Diagnosis of Syphilis, Page 1 of 2

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Abstract:

The laboratory diagnosis of syphilis has relied, in large part, upon serologic methods for over 100 years (1). The causative organism, , is not amenable to culture, and antigen detection and nucleic acid amplification techniques have not become routinely used. While many of the serologic methods in use now have been in use for decades, their application, specifically the standard testing algorithm, is changing. In order to understand the interpretation of alternative testing algorithms, a thorough understanding of test characteristics is necessary. This chapter will review serologic tests for syphilis and their interpretation when they are applied to adults with and without HIV infection and to children.

Citation: Schmitz J. 2016. Laboratory Diagnosis of Syphilis, p 412-418. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch46
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FIGURE 1

Traditional algorithm.

Citation: Schmitz J. 2016. Laboratory Diagnosis of Syphilis, p 412-418. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch46
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Image of FIGURE 2
FIGURE 2

Reverse algorithm. *, may indicate a false-positive screening test result; however, a very sensitive screening test, such as CLIA, might be positive when the standard treponemal test is nonreactive.

Citation: Schmitz J. 2016. Laboratory Diagnosis of Syphilis, p 412-418. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch46
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References

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Tables

Generic image for table
TABLE 1

Nontreponemal serologic tests for syphilis

Citation: Schmitz J. 2016. Laboratory Diagnosis of Syphilis, p 412-418. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch46
Generic image for table
TABLE 2

Common treponemal antibody tests

Citation: Schmitz J. 2016. Laboratory Diagnosis of Syphilis, p 412-418. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch46

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