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Chapter 57 : Immunologic and Molecular Methods for Viral Diagnosis

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Immunologic and Molecular Methods for Viral Diagnosis, Page 1 of 2

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Abstract:

Tremendous strides have been made in viral diagnosis in the past decade. Many infections are now treatable and, for optimum effect, antivirals must be started early. For hospitalized patients, rapid and accurate viral diagnosis is essential not only for patient management, but for infection control and prevention of nosocomial transmission. The impact of seasonal and pandemic influenza on morbidity and mortality of all ages has received increasing attention in both the medical literature and the popular press. Emerging, highly virulent viruses such as severe acute respiratory syndrome (SARS) coronavirus (CoV), Middle East respiratory syndrome CoV, and avian influenza H5N1 and H7N9 have also heightened concerns. Lastly, immunosuppressed and other vulnerable populations at risk for serious or life-threatening viral infections continue to increase. In response to these needs, there has been an explosion in viral diagnostic test development.

Citation: Landry M, Tang Y. 2016. Immunologic and Molecular Methods for Viral Diagnosis, p 538-549. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch57
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References

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Tables

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TABLE 1

Application of various methods for rapid diagnosis of acute or primary viral infections

Citation: Landry M, Tang Y. 2016. Immunologic and Molecular Methods for Viral Diagnosis, p 538-549. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch57
Generic image for table
TABLE 2

Uses and limitations of immunologic detection methods for viral antigens

Citation: Landry M, Tang Y. 2016. Immunologic and Molecular Methods for Viral Diagnosis, p 538-549. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch57
Generic image for table
TABLE 3

Mixed-cell cultures available commercially

Citation: Landry M, Tang Y. 2016. Immunologic and Molecular Methods for Viral Diagnosis, p 538-549. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch57
Generic image for table
TABLE 4

FDA-cleared molecular methods for detection, quantification, and genotyping of viral pathogens

Citation: Landry M, Tang Y. 2016. Immunologic and Molecular Methods for Viral Diagnosis, p 538-549. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch57

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