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Chapter 82 : Immunological Methods in the Diagnostic Allergy Clinical and Research Laboratory
Nearly 50 years have passed since IgE was identified as the reagin or serum antibody that sensitizes skin mast cells and circulating basophils and mediates immediate-type hypersensitivity reactions in humans (1, 2). Since IgE's identification, the clinical immunology laboratory has provided the clinician with analytical measurements that aid in the diagnosis, management, and research of the natural history and epidemiology of IgE-mediated diseases. Total and allergen-specific IgE antibody are the primary analytes measured clinically to support the diagnosis of human allergic disease. Antigen-specific IgG antibodies are measured as a research analyte to identify chronic allergen exposure and to monitor potential blocking antibody activity following immunotherapy.