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Chapter 89 : Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus

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Abstract:

This chapter deals with immunological tests that are useful in the diagnosis and monitoring of systemic lupus erythematosus (SLE). We review the detection and quantification of antibodies against self-antigens (autoantibodies), measurement of complement levels, and the interferon gene expression signature.

Citation: Reeves W, Han S, Massini J, Li Y. 2016. Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus, p 868-877. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch89
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Figures

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FIGURE 1

Protein immunoprecipitation for analyzing lupus autoantibodies. Sera were tested according to the protocol in the text, and immunoprecipitated proteins were resolved on a 12.5% gel. Sera containing the following autoantibodies were tested: anti-nRNP (RNP), anti-Sm, anti-ribosomal P (r-P), anti-proliferating cell nuclear antigen (PCNA), anti-Ki, anti-Ro60, anti-La (SS-B), anti-Ku, anti-Ku plus anti-DNA-PKcs, anti-Su, anti-RNA polymerase II (RNAPII), anti-RNA helicase A (RHA), and normal human serum (NHS). Positions of molecular weight standards are shown on the left (in kilodaltons). Protein components of the antigens are indicated (dots).

Citation: Reeves W, Han S, Massini J, Li Y. 2016. Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus, p 868-877. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch89
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References

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1. Reeves WH, Li Y, Zhuang H,. 2011. Autoantibodies in systemic lupus erythematosus, p 12791288. In Hochberg MC, Silman AJ, Smolen JS, Weinblatt ME, Weisman MH (ed), Rheumatology, 5th ed. Mosby Elsevier, Philadelphia, PA.[CrossRef]
2. Craft J. 1992. Antibodies to snRNPs in systemic lupus erythematosus. Rheum Dis Clin North Am 18:311335.[PubMed]
3. Elkon KB, Bonfa E, Brot N. 1992. Antiribosomal antibodies in systemic lupus erythematosus. Rheum Dis Clin North Am 18:377390.[PubMed]
4. Fritzler MJ, McCarty GA, Ryan JP, Kinsella TD. 1983. Clinical features of patients with antibodies directed against proliferating cell nuclear antigen. Arthritis Rheum 26:140145.[PubMed].[CrossRef]
5. Yamasaki Y, Narain S, Yoshida H, Hernandez L, Barker T, Hahn PC, Sobel ES, Segal MS, Richards HB, Chan EK, Reeves WH, Satoh M. 2007. Autoantibodies to RNA helicase A: a new serologic marker of early lupus. Arthritis Rheum 56:596604.[CrossRef].[PubMed]
6. Mattioli M, Reichlin M. 1974. Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen. Arthritis Rheum 17:421429.[PubMed].[CrossRef]
7. Ho KT, Reveille JD. 2003. The clinical relevance of autoantibodies in scleroderma. Arthritis Res Ther 5:8093.[PubMed]
8. Lerner MR, Steitz JA. 1979. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 76:54955499.[PubMed].[CrossRef]
9. Arbuckle MR, McClain MT, Rubertone MV, Scofield RH, Dennis GJ, James JA, Harley JB. 2003. Development of autoantibodies before the clinical onset of systemic lupus erythematosus. N Engl J Med 349:15261533.[CrossRef].[PubMed]
10. Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ. 1982. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 25:12711277.[PubMed].[CrossRef]
11. Buyon JP, Clancy RM, Friedman DM. 2009. Cardiac manifestations of neonatal lupus erythematosus: guidelines to management, integrating clues from the bench and bedside. Nat Clin Pract Rheumatol 5:139148.[CrossRef].[PubMed]
12. Hanly JG, Urowitz MB, Siannis F, Farewell V, Gordon C, Bae SC, Isenberg D, Dooley MA, Clarke A, Bernatsky S, Gladman D, Fortin PR, Manzi S, Steinsson K, Bruce IN, Ginzler E, Aranow C, Wallace DJ, Ramsey-Goldman R, van Vollenhoven R, Sturfelt G, Nived O, Sanchez-Guerrero J, Alarcón GS, Petri M, Khamashta M, Zoma A, Font J, Kalunian K, Douglas J, Qi Q, Thompson K, Merrill JT Systemic Lupus International Collaborating Clinics. 2008. Autoantibodies and neuropsychiatric events at the time of systemic lupus erythematosus diagnosis: results from an international inception cohort study. Arthritis Rheum 58:843853.[CrossRef].[PubMed]
13. Burbelo PD, Ching KH, Issa AT, Loftus CM, Li Y, Satoh M, Reeves WH, Iadarola MJ. 2009. Rapid serological detection of autoantibodies associated with Sjögren's syndrome. J Transl Med 7:83.[CrossRef].[PubMed]
14. Weinstein A, Bordwell B, Stone B, Tibbetts C, Rothfield NF. 1983. Antibodies to native DNA and serum complement (C3) levels. Application to diagnosis and classification of systemic lupus erythematosus. Am J Med 74:206216.[PubMed].[CrossRef]
15. Ter Borg EJ, Horst G, Hummel EJ, Limburg PC, Kallenberg CGM. 1990. Measurement of increases in anti-double-stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus. A long-term, prospective study. Arthritis Rheum 33:634643.[PubMed].[CrossRef]
16. Bootsma H, Spronk P, Derksen R, de Boer G, Wolters-Dicke H, Hermans J, Limburg P, Gmelig-Meyling F, Kater L, Kallenberg C. 1995. Prevention of relapses in systemic lupus erythematosus. Lancet 345:15951599.[PubMed].[CrossRef]
17. Pickering MC, Botto M, Taylor PR, Lachmann PJ, Walport MJ. 2000. Systemic lupus erythematosus, complement deficiency, and apoptosis. Adv Immunol 76:227324.[PubMed]
18. Ornstein BW, Atkinson JP, Densen P. 2012. The complement system in pediatric systemic lupus erythematosus, atypical hemolytic uremic syndrome, and complocentric membranoglomerulopathies. Curr Opin Rheumatol 24:522529.[CrossRef].[PubMed]
19. Wen L, Atkinson JP, Giclas PC. 2004. Clinical and laboratory evaluation of complement deficiency. J Allergy Clin Immunol 113:585593.[CrossRef].[PubMed]
20. Grotz W, Baba HA, Becker JU, Baumgärtel MW. 2009. Hypocomplementemic urticarial vasculitis syndrome: an interdisciplinary challenge. Dtsch Arztebl Int 106:756763.[CrossRef].[PubMed]
21. Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Ortmann WA, Espe KJ, Shark KB, Grande WJ, Hughes KM, Kapur V, Gregersen PK, Behrens TW. 2003. Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus. Proc Natl Acad Sci USA 100:26102615.[CrossRef].[PubMed]
22. Bennett L, Palucka AK, Arce E, Cantrell V, Borvak J, Banchereau J, Pascual V. 2003. Interferon and granulopoiesis signatures in systemic lupus erythematosus blood. J Exp Med 197:711723.[CrossRef].[PubMed]
23. Feng X, Wu H, Grossman JM, Hanvivadhanakul P, FitzGerald JD, Park GS, Dong X, Chen W, Kim MH, Weng HH, Furst DE, Gorn A, McMahon M, Taylor M, Brahn E, Hahn BH, Tsao BP. 2006. Association of increased interferon-inducible gene expression with disease activity and lupus nephritis in patients with systemic lupus erythematosus. Arthritis Rheum 54:29512962.[CrossRef].[PubMed]
24. Hua J, Kirou K, Lee C, Crow MK. 2006. Functional assay of type I interferon in systemic lupus erythematosus plasma and association with anti-RNA binding protein autoantibodies. Arthritis Rheum 54:19061916.[CrossRef].[PubMed]
25. Li Y, Lee PY, Kellner ES, Paulus M, Switanek J, Xu Y, Zhuang H, Sobel ES, Segal MS, Satoh M, Reeves WH. 2010. Monocyte surface expression of Fcgamma receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus. Arthritis Res Ther 12:R90.[CrossRef].[PubMed]
26. Kellner ES, Lee PY, Li Y, Switanek J, Zhuang H, Segal MS, Sobel ES, Satoh M, Reeves WH. 2010. Endogenous type-I interferon activity is not associated with depression or fatigue in systemic lupus erythematosus. J Neuroimmunol 223:1319.[CrossRef].[PubMed]

Tables

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TABLE 1

Some non-DNA/chromatin autoantibodies useful in the diagnosis of systemic autoimmune disease

Citation: Reeves W, Han S, Massini J, Li Y. 2016. Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus, p 868-877. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch89
Generic image for table
TABLE 2

Prevalence of some non-DNA/chromatin autoantibodies by disease

Citation: Reeves W, Han S, Massini J, Li Y. 2016. Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus, p 868-877. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch89

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