1887

Chapter 90 : Immunodiagnosis of Autoimmune Myopathies

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Immunodiagnosis of Autoimmune Myopathies, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818722/9781555818715_CH90-1.gif /docserver/preview/fulltext/10.1128/9781555818722/9781555818715_CH90-2.gif

Abstract:

Certain autoantibodies can be clinically useful biomarkers associated with a particular disease and/or clinical features. Some of them, called disease marker antibodies, are highly specific for a particular diagnosis and have predictive value for the development of the disease, and they are included in classification criteria for systemic autoimmune rheumatic diseases (1, 2). The majority of disease-associated autoantibodies in systemic lupus erythematosus and scleroderma (systemic sclerosis) have been known for decades. However, autoantibody research in polymyositis/dermatomyositis (PM/DM) has been highly active in recent years as several new, clinically important autoantibody specificities with strong clinical impact have been identified, such as antibodies to transcription intermediary factor 1γ/α (TIF1γ/α; p155/140), which are frequently found in cancer-associated DM (3–5); and to melanoma differentiation-associated gene 5 (MDA5), associated with clinically amyopathic DM (CADM) with rapidly progressive interstitial lung disease (ILD) (6–9). Autoantibodies that are found in PM/DM are often classified into myositis-specific autoantibodies (MSAs) and myositis-associated autoantibodies (10, 11). MSAs are found almost exclusively in PM/DM among systemic rheumatic diseases, although some are also found in patients classified into idiopathic ILD, such as anti-PL-12 and anti-KS antibodies (12, 13).

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of FIGURE 1
FIGURE 1

IP analysis of protein components of autoantigens recognized by autoantibodies in PM/DM. [S]Methionine-labeled K562 cell extract was immunoprecipitated by sera from patients with PM/DM. The main components of each autoantigen are indicated by arrowheads or a line drawn on the right (Mi-2). Jo-1, histidyl-tRNA synthetase; PL-7, threonyl-tRNA synthetase; EJ, glycyl-tRNA synthetase; PL-12, alanyl-tRNA synthetase; OJ, isoleucyl-tRNA synthetase multienzyme complex; SMN, survival of motor neuron; NHS, normal human serum; MW, molecular weight.

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of FIGURE 2
FIGURE 2

IP analysis of RNA components of autoantigens recognized by autoantibodies. K562 cell extract was immunoprecipitated by autoimmune sera. RNA components were extracted, fractionated on urea-PAGE, and silver stained. RNA components of autoantigen are indicated by arrowheads. Sm (U1, 2, 4-6, 5), U1, 2, 4-6, 5 small nuclear RNAs; U1RNP (U1), U1 small nuclear RNA; U2RNP (U2), U2 small nuclear RNA; U3RNP (U3), U3 small nucleolar RNA; Th (7-2, 8-2), 7-2 and 8-2 RNAs; SRP (7SL), 7SL RNAs; NHS, normal human serum. Y1-5 RNAs are also seen in the anti-OJ lane due to coexisting anti-Ro60 antibodies.

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555818722.ch90
1. Satoh M, Chan EK, Sobel ES, Kimpel DL, Yamasaki Y, Narain S, Mansoor R, Reeves WH. 2007. Clinical implication of autoantibodies in patients with systemic rheumatic diseases. Expert Rev Clin Immunol 3:721738.[CrossRef].[PubMed]
2. Satoh M, Vázquez-Del Mercado M, Chan EK. 2009. Clinical interpretation of antinuclear antibody tests in systemic rheumatic diseases. Mod Rheumatol 19:219228.[CrossRef].[PubMed]
3. Targoff IN, Mamyrova G, Trieu EP, Perurena O, Koneru B, O'Hanlon TP, Miller FW, Rider LG Childhood Myositis Heterogeneity Study Group, International Myositis Collaborative Study Group. 2006. A novel autoantibody to a 155-kd protein is associated with dermatomyositis. Arthritis Rheum 54:36823689.[CrossRef].[PubMed]
4. Fujimoto M, Hamaguchi Y, Kaji K, Matsushita T, Ichimura Y, Kodera M, Ishiguro N, Ueda-Hayakawa I, Asano Y, Ogawa F, Fujikawa K, Miyagi T, Mabuchi E, Hirose K, Akimoto N, Hatta N, Tsutsui K, Higashi A, Igarashi A, Seishima M, Hasegawa M, Takehara K. 2012. Myositis-specific anti-155/140 autoantibodies target transcription intermediary factor 1 family proteins. Arthritis Rheum 64:513522.[CrossRef].[PubMed]
5. Satoh M, Chan JY, Ross SJ, Li Y, Yamasaki Y, Yamada H, Vazquez-del Mercado M, Petri MH, Jara LJ, Saavedra MA, Cruz-Reyes C, Sobel ES, Reeves WH, Ceribelli A, Chan EK. 2012. Autoantibodies to transcription intermediary factor TIF1β associated with dermatomyositis. Arthritis Res Ther 14:R79. doi:10.1186/ar3802.[CrossRef].[PubMed] http://dx.doi.org/doi:10.1186/ar3802
6. Sato S, Hoshino K, Satoh T, Fujita T, Kawakami Y, Fujita T, Kuwana M. 2009. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: association with rapidly progressive interstitial lung disease. Arthritis Rheum 60:21932200.[CrossRef].[PubMed]
7. Sato S, Hirakata M, Kuwana M, Suwa A, Inada S, Mimori T, Nishikawa T, Oddis CV, Ikeda Y. 2005. Autoantibodies to a 140-kd polypeptide, CADM-140, in Japanese patients with clinically amyopathic dermatomyositis. Arthritis Rheum 52:15711576.[CrossRef].[PubMed]
8. Hoshino K, Muro Y, Sugiura K, Tomita Y, Nakashima R, Mimori T. 2010. Anti-MDA5 and anti-TIF1-γ antibodies have clinical significance for patients with dermatomyositis. Rheumatology (Oxford) 49:17261733.[CrossRef].[PubMed]
9. Nakashima R, Imura Y, Kobayashi S, Yukawa N, Yoshifuji H, Nojima T, Kawabata D, Ohmura K, Usui T, Fujii T, Okawa K, Mimori T. 2010. The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology (Oxford) 49:433440.[CrossRef].[PubMed]
10. Targoff IN. 2000. Update on myositis-specific and myositis-associated autoantibodies. Curr Opin Rheumatol 12:475481.[PubMed].[CrossRef]
11. Nakashima R, Mimori T. 2010. Clinical and pathophysiological significance of myositis-specific and myositis-associated autoantibodies. Int J Clin Rheumatol 5:523536.[CrossRef]
12. Targoff IN, Arnett FC. 1990. Clinical manifestations in patients with antibody to PL-12 antigen (alanyl-tRNA synthetase). Am J Med 88:241251.[PubMed].[CrossRef]
13. Hirakata M, Suwa A, Nagai S, Kron MA, Trieu EP, Mimori T, Akizuki M, Targoff IN. 1999. Anti-KS: identification of autoantibodies to asparaginyl-transfer RNA synthetase associated with interstitial lung disease. J Immunol 162:23152320.[PubMed]
14. Targoff IN. 2002. Laboratory testing in the diagnosis and management of idiopathic inflammatory myopathies. Rheum Dis Clin North Am 28:859890, viii.[PubMed].[CrossRef]
15. Reeves WH, Satoh M, Lyons R, Nichols C, Narain S,. 2006. Detection of autoantibodies against proteins and ribonucleoproteins by double immunodiffusion and immunoprecipitation, p 10071018. In Rose NR, Hamilton RG, Detrick B, Reeves WH (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th ed. ASM Press, Washington, DC.
16. Betteridge ZE, Gunawardena H, McHugh NJ. 2011. Novel autoantibodies and clinical phenotypes in adult and juvenile myositis. Arthritis Res Ther 13:209. doi:10.1186/ar3275.[CrossRef].[PubMed] http://dx.doi.org/doi:10.1186/ar3275
17. Targoff IN. 2008. Autoantibodies and their significance in myositis. Curr Rheumatol Rep 10:333340.[PubMed].[CrossRef]
18. Mammen AL. 2011. Autoimmune myopathies: autoantibodies, phenotypes and pathogenesis. Nat Rev Neurol 7:343354.[CrossRef].[PubMed]
19. Ceribelli A, Fredi M, Taraborelli M, Cavazzana I, Franceschini F, Quinzanini M, Tincani A, Ross SJ, Chan JY, Pauley BA, Chan EK, Satoh M. 2012. Anti-MJ/NXP-2 autoantibody specificity in a cohort of adult Italian patients with polymyositis/dermatomyositis. Arthritis Res Ther 14:R97. doi:10.1186/ar3822.[CrossRef].[PubMed] http://dx.doi.org/doi:10.1186/ar3822

Tables

Generic image for table
TABLE 1

Identification of myositis autoantibodies by IP analysis

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Generic image for table
TABLE 2

Prevalence and clinical association of myositis autoantibodies

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Generic image for table
Untitled

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Generic image for table
Untitled

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Generic image for table
Untitled

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90
Generic image for table
Untitled

Citation: Satoh M, Ceribelli A, Hirakata M, Chan E. 2016. Immunodiagnosis of Autoimmune Myopathies, p 878-887. In Detrick B, Schmitz J, Hamilton R (ed), Manual of Molecular and Clinical Laboratory Immunology, Eighth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818722.ch90

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error