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Chapter 12.4 : Sequence-Based Identification and Typing
Category: Clinical Microbiology
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Left panels represent electropherograms before normalization, with marker peaks illustrated (open circles). Right panels represent normalized electropherograms, with marker peaks removed. A, low, broad markers; B, low-intensity electropherogram.
Normalized electropherogram for Staphylococcus aureus with several high-intensity peaks.
Overlay of two related strains of Staphylococcus aureus illustrating peak differences (arrows).
Dendogram of a cluster of Staphylococcus aureus infections. P = patterns representing identical isolates with >97% similarity and no band differences. G = group indicating related isolates with 95-97% similarity and one band difference. Bracket illustrates related or identical isolates. Arrows indicate band differences between isolates.
Similarity matrix of Staphylococcus aureus cluster.
Scatter plot of Staphylococcus aureus cluster. The circled numbers illustrate the related isolates.
PFGE analysis of S. aureus chromosomal DNA digested with SmaI. Isolates 1, 2, and 3 differ by up to three bands, indicating they are similar. Isolate 4 differs from isolates 1 to 3 by ≥7 bands, indicating they are different and likely unrelated.
PFGE analysis of Klebsiella pneumoniae chromosomal DNA digested with XbaI. Isolates 2 and 3 are indistinguishable and differ from isolate 1 by only two bands, indicating they are similar. Isolate 4 differs from isolates 1 to 3 by ≥7 bands, indicating they are different and likely unrelated.
Selected available reagents, kits, and software for sequence-based identification of bacteria or fungi
Example electrophoresis conditions for selected organisms a
The seven loci and primers for multilocus sequence typing of Staphylococcus aureus located at http://www.mlst.net/